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738 results for “Microsatellites”
Fig. 1 in Inheritance of fifeen microsatellite loci in Ceratitis capitata (Diptera: Tephritidae)
Fig. 1. Sequence alignment of Ccmic3 with original primer design. Top two sequences are genotypes of 74 bp allele and bottom two sequences are genotypes of the 76 bp allele. One extra thymine residue on the 5' end appeared in 8 of the 8 clones for this allele leading to difficulties in scoring this locus. Redesign of the forward primer by adding the extra guanines provided better resolution in scoring. Lower case sequence represents the Topo 2.1 vector just past the EcoRI in the multiple cloning site.
Fig. 3 in Inheritance of fifeen microsatellite loci in Ceratitis capitata (Diptera: Tephritidae)
Fig. 3. Comparing artifactual bands to actual bands to determine allele as observed at the Ccmic25 locus. a) Chromatograph of sample exhibiting an artifactual band can be observed at the 136 bp peak. The geometric shape and configuration is not consistent with those of microsatellites. b) This chromatograph shows the characteristic high peak followed by a low peak expected when making a call around the 137 bp peak. To the lef of both peaks are very low broad bands, commonly observed during analysis. In homozygote individuals, these peaks may extend higher and resemble an artifactual band similar to what was observed in Fig. 3a.
Fig. 2 in Testing microsatellite loci and preliminary genetic study for Eurasian otter in South Korea
Fig. 2. Locations of sampling for tissue (1. Hoengseong-gun, Gangwon-do, 2. Uljin-gun, Gyeongsangbuk-do, 3. Jeongeup-si, Jeollabukdo, 4. Muju-gun, Jeollabuk-do, 5. Hampyeong-gun, Jeollanam-do).
Fig. 2 in Genetic diversity and population structure of endangered Neofinetia falcata (Orchidaceae) in South Korea based on microsatellite analysis
Fig. 2. Structure analyses for putative genetic clusters of N. falcata. A: Graphs of ΔK values to determine the ideal number of groups present in the accessions of N. falcata. B: Estimated genetic structure of the 3 populations of brinjal based on STRUCTURE analysis K = 2 and K = 3.
Figure 4 in Microsatellite based genetic diversity of Mediterranean fruit fly (Ceratitis capitata, Diptera: Tephritidae) populations from Southwest Turkey
Figure 4. ΔK distribution along with different values of clusters (K) for 7 populations depending on Evanno's method (Evanno et al. 2005) using Structure Harvester application.
Figure 2 in Microsatellite based genetic diversity of Mediterranean fruit fly (Ceratitis capitata, Diptera: Tephritidae) populations from Southwest Turkey
Figure 2. Unrooted Neighboor-Joinning (NJ) tree of 7 C. capitata populations using 8 polymorphic microsatellite markers.
Fig. 2 in Heterochromatin distribution and chromosomal mapping of microsatellite repeats in the genome of Frieseomelitta stingless bees (Hymenoptera: Apidae: Meliponini)
Fig. 2. Metaphase spreads of females of Frieseomelitta varia (a, g), Frieseomelitta sp. n. (b, h), Frieseomelitta meadewaldoi (c, i), Frieseomelitta dispar (d, j), Frieseomelitta francoi (e, k), and Frieseomelitta doederleini (f, l) afer basespecific fluorochrome staining. The arrows indicate the GC-rich regions.
Fig. 3 in Heterochromatin distribution and chromosomal mapping of microsatellite repeats in the genome of Frieseomelitta stingless bees (Hymenoptera: Apidae: Meliponini)
Fig. 3. Metaphase spreads of females of Frieseomelitta species afer fluorescence in situ hybridizaton with microsatellite probes.
Fig. 1. C in Heterochromatin distribution and chromosomal mapping of microsatellite repeats in the genome of Frieseomelitta stingless bees (Hymenoptera: Apidae: Meliponini)
Fig. 1. C-banded karyotypes of females of Frieseomelitta varia (a), Frieseomelitta doederleini (b), Frieseomelitta sp. nov. (c), Frieseomelitta meadewaldoi (d), Frieseomelitta dispar (e), and Frieseomelitta francoi (f). (M = metacentric, A = acrocentric, MT = metacentric with centromeric, and telomeric C-bands, AM = pseudoacrocentric).
Fig. 1. Maximum likelihood tree generating from a 399 in Isolation and Characterization of Polymorphic Microsatellite Loci for Caridina cantonensis and Transferability Across Eight Confamilial Species (Atyidae, Decapoda)
Fig. 1. Maximum likelihood tree generating from a 399-bp long COI dataset (GenBank accession no. MH176649-MH176993). SH-alrt/ bootstrap support values are indicated at major nodes. Each coloured notation represents one species.
FIGURE 2 in Development of microsatellite loci and population genetics of the catfish Pimelodus yuma (Siluriformes: Pimelodidae)
FIGURE 2 | Discriminant analysis of principal components for nine microsatellite loci and 138 individuals of Pimelodus yuma in three sections (S4/5, S6 and S7/8) of the Cauca River.
FIGURE 1 in Development of microsatellite loci and population genetics of the catfish Pimelodus yuma (Siluriformes: Pimelodidae)
FIGURE 1 | Studied sampling sites of Pimelodus yuma along the lower sections (S4–S8) of the Cauca River. The pentagons indicate sampling sites in floodplain lakes and the stars indicate sites along the main channel of the river.
FIGURE 2 in Population genetics of the endangered catfish Pseudoplatystoma magdaleniatum (Siluriformes: Pimelodidae) based on species-specific microsatellite loci
FIGURE 2 | Results of Structure (A, B) and Discriminant analysis of principal components (C) for Pseudoplatystoma magdaleniatum. A: K = 1; B: K = 2; M: Margento, PC: Punta Cartagena, PB: Puerto Berrío, SN: Samaná Norte.
FIGURE 3 in Development of microsatellite loci and population genetics of the catfish Pimelodus yuma (Siluriformes: Pimelodidae)
FIGURE 3 | STRUCTURE results for Pimelodus yuma showing K= 2 genetic stocks in three sections (S4/5, S6 and S7/8) of the Cauca River.
Microsatellite data for Aedes aegypti populations in Florida and southern California
<p>In the affiliated paper we compare likely the oldest populations of <i>Aedes aegypti</i> in continental North America with some of the newest to illuminate the range of genetic diversity and structure that can be found within the invasive range of this important disease vector. <i>Aedes aegypti</i> populations in Florida have likely persisted since the 1600-1700s, while populations in southern California derive from new invasions that occurred in the last ten years. For this comparison, we genotyped 1,193 individuals from 29 sites at 12 highly variable microsatellites and a subset of these individuals at 23,961 single nucleotide polymorphisms (SNPs).</p>
Fig. 2 in Microsatellite variation and population genetic structure of a neotropical endangered Bryconinae species Brycon insignis Steindachner, 1877: implications for its conservation and sustainable management
Fig. 2. UPGMA clustering of the Nei's genetic distance (1972) of the Brycon insignis sampling locations based on six microsatellite loci. Bootstrap values above 50% are shown above branches indicating percentage support in 5000 permutations. Power Company Hatchery (PCH), São João River (SJR), Paraíba do Sul River (PSR), Imbé River (IMR), Muriaé River (MUR) and Itabapoana River (ITR).
Microsatellite dataset from: The fast invasion of Europe by the box tree moth: An additional example coupling multiple introduction events, bridgehead effects and admixture events.
<p>Genotypes of native and invasive populations of Cydalima perspectalis for 15 microsatellites generated for our paper "The fast invasion of Europe by the box tree moth: An additional example coupling multiple introduction events, bridgehead effects and admixture events."</p> <p>This folder contains:<br> - ReadMe file giving the following information</p> <p>- "Data_Cydalima_genotype.xlsx” containing populations information and individuals genotype for the 15 microsatellites developed by Bras et al. 2018 (Eur. J. Entomol.)</p> <p> </p> <p>This work was done in the frame of a regional project called INCA (grant from Région Centre Val de Loire).</p>
Microsatellite data for Gekko hokouensis and G. yakuensis from southern Kyushu and Tanegashima Island, Japan
<p><strong>README_file.txt</strong></p> <p>This file explains the contents of the dataset.</p> <p> </p> <p><strong>Microsatellite_data_for_Gekko_hokouensis_and_G._yakuensis.csv</strong></p> <p>This file is in STRUCTURE format and contains the raw data of allele lengths collected from 330 individuals using microsatellite genotyping. The first line lists the names of 16 microsatellite loci. The first column lists sample IDs, and the second column lists the names of sampling sites. Two alleles for each sample are listed in two consecutive columns in the subsequent columns. Missing data is represented with -9. These data include genotypic data for 20 pure <em>G. hokouensis</em> and 20 pure <em>G. yakuensis</em> used in Okamoto et al. (2020).</p>
Waste not, want not: microsatellites remain an economical and informative technology for conservation genetics
<p>Comparisons of microsatellite and single-nucleotide polymorphisms (SNPs) have found that SNPs outperform microsatellites in population genetic analyses, calling into question the continued utility of microsatellites in population and landscape genetics. Yet highly polymorphic markers may be of value in species that have reduced genetic variation. This study repeated analyses previously done using microsatellites with SNPs developed from ddRAD sequencing in the black-capped vireo source-sink system. SNPs provided greater resolution of genetic diversity, population differentiation, and migrant detection but could not reconstruct parentage relationships due to insufficient heterozygosities. The biological inferences made by both sets of markers were similar: asymmetrical gene flow from source sites to the remaining sink sites. With the landscape genetic analyses, we found different results between the two molecular markers, but associations of the top environmental features (riparian, open habitat, agriculture, and human development) with dispersal estimates were shared between marker types. Despite the higher precision of SNPs, we find that microsatellites effectively uncover population processes and patterns and are superior for parentage analyses in this species with reduced genetic diversity. This study illustrates the continued applicability and relevance of microsatellites in population genetic research.</p>
Microsatellite data of the paper "A putatively new ant species from the Cataglyphis cursor group displays low levels of polyandry with standard sexual reproduction"
<p>Fifty colonies of the ant Cataglyphis cursor were sampled at their nest entrance in two localities separated by 79km in the plain of Avila, west of Madrid, in July 2015 for Salobralejo (27 colonies) and in April 2014 for Castrillo de Guareña (23 colonies). Only workers near the nest entrance were collected. In Salobralejo, we also collected nine gynes at the nest entrance in three colonies. After collection, the individuals were preserved in 95% Ethanol (with 5% Tris-EDTA). From the 50 colonies sampled, we genotyped a single worker per colony for 37 colonies (Table S1). For the other 13 colonies, 114 workers were collected and genotyped to assess the colony genetic structure (86 workers from nine colonies in Salobralejo and 28 workers from four colonies in Castrillo). We also genotyped the nine gynes found in three colonies in Salobralejo. A total of 160 individuals were screened for thirteen microsatellite loci used by Eyer et al. (2023) and two supplementary loci (L76 and L3653) were amplified. The L26 locus was excluded because of amplification failures in nine individuals (18% of samples) and a highly significant Hardy-Weinberg disequilibrium (<em>P</em> < 00001 for both sites). </p> <p>The file contain the locality (Salobralejo or Castrillo), the colony number and individual identification number (code), the caste (worker or gyne). For each locus, the two alleles are provided and characterised by their size. </p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
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