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Measurements of coarse woody debris at the Coweeta LTER Terrestrial Gradient Sites, Coweeta Hydrological Laboratory, Otto, NC from 2003 to 2014

The five Terrestrial Gradient sites were established in the early 1990s as part of the 1990 Coweeta LTER Renewal. The original terrestrial gradient sites were 20 x 40-m. In the late 1990s the plots were expanded to 80 x 80-m and later (around 1998) they were slope-corrected by Clark's lab using survey equipment. In this study, coarse woody debris (CWD) was measured in each of the five terrestrial gradient plots at the Coweeta Hydrologic Lab from 2003 through 2014. The length, diameters, and decay class of coarse wood were measured within each of the plots.

Measurements of Soil %C and %N at the Coweeta LTER Terrestrial Gradient Sites, Coweeta Hydrological Laboratory, Otto, NC.

This project is part of a larger examination of site productivity along an elevational gradient. Soil %C and %N were measured at each of the five terrestrial gradient plots located along an elevational gradient at Coweeta Hydrologic Lab, Otto, NC.

Spring and Fall Leaf Phenology from Coweeta LTER Soil Moisture Sites SM2 & SM4, Coweeta Hydrologic Laboratory, Otto, NC, 2003-2015

Spring vegetative bud break, leaf elongation, fall leaf color, and leaf senescence are monitored at the two scaffold towers located at Project 1040 soil moisture microclimate sites 2 and 4. We have identified a variety of species at the elevation extremes within the Coweeta basin for this yearly monitoring project.

Measurements of leaf litter Carbon and Nitrogen from the Coweeta LTER Terrestrial Gradient sites, Coweeta Hydrological Laboratory, Otto, NC

This project was started by Haines and Crossley in 1992 to compare leaf litter weights among the five gradient plots (though data from the first collections were not included in this dataset because the original dataset did not represent a complete quarter of collection). Litter is collected from ten 91.4 x 91.4 cm leaf collectors within each of the gradient plots. Litter is collected on a quarterly basis (monthly in the autumn). The litter is then separated by category, dried, weighed, and processed in a Wiley mill for later carbon and nitrogen analyses. Since the project first started, the collection process has expanded to include non-leaf litter (e.g., lichens, bark, and seeds), fine twigs (0-2.5 cm diameter), and medium twigs (2.5-10 cm diameter).

Soil respiration and flux measurements from Watersheds 17 and 18,Coweeta Hydrologic Laboratory, Otto, NC.

In order to better understand soil respiration and Carbon Dioxide (CO2) fluxes, 90 closed container chambers were installed in 18 plots located at upper, mid, and lower-slope lcoations within Coweetas watersheds 17 and 18. For eighteen months, soil moisture, soil temperature, and CO2 measurements were taken through the collars of pipes located within each plots. These measurements were used to determine total flux and other statisitcal information in order to better understand these forest processes.

Microclimate data from stations within the Great Smoky Mountains National Park, NC

This dataset contains measurements of air temperature, relative humidity, soil temperature, soil bulk electrical conductivity, and soil volumetric water content from a sites located in the Great Smoky Mountains National Park, NC. Soil temperature, soil bulk electrical conductivity, and soil volumetric water are measured at 0-30cm and 30-60cm below ground surface at locations 25 meters above gradient and 25 meters below gradient of a central transect location where air temperature and relative humidity are measured at 1.5 meters above ground surface. Measurements are taken every 60 seconds with average, minimum, and maximum values saved hourly to the output table.

Long term responses of first order streams to allochthonous and detrital manipulations at the Coweeta Hydrologic Laboratory, Otto, NC from 1984 to 2006

This dataset examines the long term effects of litter exclusion, small and large wood removal, and the addition of leaf species of varying detrital quality on organic matter standing crop, export of organic and inorganic particles, and invertebrate abundance and biomass in a high-gradient headwater stream. Pre-treatment and stream reference data are also included. This study was conducted at the Coweeta Hydrologic Laboratory watersheds 53, 54, and 55 from years 1988 through 2006.

Hourly water quality measurements from 21 sites in the Little Tennessee River watershed, Macon County, NC

Water quality was monitored at nine intensive sites, nine hillslope sites, and three river sites in the Little Tennnesse River watershed, Macon County, NC in 2010-2013. The intensive sites were monitored in 2010-2011, the hillslope sites were monitored in 2012-2013, and the river sites were monitored 2010-2013. A Hach Hydrolab MS5 data sonde measured stream temperature, specific conductivity, dissolved oxygen, and turbidity every hour.

Water Level, Water Temperature, and Flow Measurements from the Ball Creek weir house #9, Coweeta Hydrologic Laboratory, Otto, NC.

This data set contains water level, water temperature, and calculated discharge values from the Ball Creek weir house #9, Coweeta Hydrologic Laboratory, Otto, NC. Using a pressure transducer, measurements are taken every 60 seconds with the average, minimum, and maximum values for water pressure and water temperature saved to the output data table hourly. Water level and discharge are calculated using the hourly average water pressure, and saved to the online data files.

Salamander survival and growth cage experiment at the Coweeta Hydrologic Laboratory, Otto, NC

Climate change is predicted to alter biotic communities and, as a result, cause changes in ecosystem processes. Such predictions assume that future communities will lack species capable of compensating for the loss of other species. In southern Appalachian headwater streams, abundant larval Black-bellied Salamanders (Desmognathus quadramaculatus) represent a significant standing crop of nitrogen (N) and phosphorus (P). Desmognathus quadramaculatus are projected to be extirpated from the southern Appalachian highlands under most climate change scenarios, which would result in the loss of most salamander standing crop of limiting nutrients unless other species compensate for the loss of D. quadramaculatus biomass. Eurycea cirrigera, which has an abundant congener Eurycea wilderae already in the headwaters, and Gyrinophilus porphyriticus, which currently occurs in low densities in the headwaters, are projected to remain within southern Appalachian highlands. We used field cages to measure (1) the amount of compensatory survival and growth Eurycea would show in the absence of the larger, predatory D. quadramaculatus, and (2) whether replacement of D. quadramaculatus by G. porphyriticus, which is known to be a more efficient predator, would reduce Eurycea and total salamander biomass.

Desmognathus quadramaculatus excretion rate study at the Coweeta Hydrologic Laboratory, Otto, NC.

We examined the excretion rate of 18 Desmognathus quadramaculatus collected from W34 at Coweeta. Salamanders were collected and immediately placed in filtered stream water. They remained in the water for 24 hrs. Following excretion trials, animals were sacrificed, remaining water was filtered, and each was analyzed for levels of nitrogen and phosphorus.

Concentration of calcium in stream-dwelling Plethodontid salamanders across six streams located with the Coweeta Hydrologic Laboratory, Otto, NC

We examined the concentration of calcium in several species of stream-dwelling plethodontid salamanders captured across six streams located within the Coweeta LTER site. %Ca was measured for 15 Eurycea wilderae, 15 Desmognathus ocoee, and 20 Desmognathus quadramaculatus. Because D. quadramaculatus larvae represent animals across a 3-4 year larval lifespan, for %Ca analysis we randomly sampled 6-8 D. quadramaculatus from each of three size classes: 18-25 mm SVL, 26-33 mm SVL, and 33-40 mm SVL.

Plethodon hybrid zone capture-mark-recapture survey plots at the Coweeta Hyrdologic Laboratory, Otto, NC.

A major goal of the Coweeta LTER is to understand the interactions between climate and land use on the ecology of southern Appalachia biota. Southern Appalachia is the global hotspot for salamander diversity, with most of that diversity situated at mid and upper elevations of mountains where species are functionally trapped by their dependence of a narrow, cool climatic zone. The ranges of the two terrestrial salamander species, Plethodon teyahalee (southern Appalachian salamander) and Plethodon shermani (red-legged salamander) meet at a unique hybrid zone at the Coweeta LTER in Macon County, NC. The hybrid zone is unique because it appears to be shifting up in elevation, possibly due to climate change. This research will be situated in the hybrid zone of these two species to assess the differential effects of climate change and land use on both species. To evaluate the effects of climate on the local ecology of salamanders, we will conduct an intensive mark-recapture study to measure surface activity on 6 plots distributed in pairs at 3 elevations that provide a range of warmer to cooler climates. Every two weeks, each plot is searched for 30 min by two investigators using head lamps. Animals are hand captured, identified to species, scored for color and patterning, measured, and marked using a unique combination of visible implant elastomers (VIE). This will enable us to estimate individual capture probabilities and rates of temporary surface emigration (an indication of avoidance of climatically unsuitable conditions).

Stream sampling for total suspended solids (TSS), volatile suspended solids (VSS), and chemistry during storm events at the Coweeta LTER intensive and hillslope sites in Macon County, NC.

Stream storm samples were collected at 21 streams and rivers in Macon County, NC. Nine intensive sites were monitored in 2010-2011, nine hillslope sites were monitored in 2012-2013, and three river sites were monitored from 2010-2013. An ISCO water sampler was used to collect stream water samples during storm events. Water samples were analyzed at the Coweeta Analytical Lab.

opencc-by-nc-4.0Mar 2023View details →

US National Native Bee Monitoring RCN Data Management Workshop: CC BY NC Videos

<p>The US National Native Bee Monitoring Research Coordination Network (RCN) held a two-day workshop on data management best practices for native bee inventory, survey, and monitoring data on March 28 and 30, 2023. Videos in this data set were played at the workshop. These videos are released with a CC BY-NC license. These videos are for non-commercial use only. Please cite the presenter(s) of the video(s) you use. This data set includes the following videos: </p><ul><li>APHIS National Honey Bee Pests and Diseases Survey by Anne LeBrun</li><li>Importance of FAIR in Biodiversity Science and Research by Elizabeth R. Ellwood</li><li>Bugflow: A Community Driven Repository for Entomology Digitization Resources by Crystal Maier</li><li>A Primer on iNaturalist Bee Data by Keng-Lou James Hung, Paige Chesshire, Michael Orr, Alice Hughes, Jess Mullins, Katherine Parys, Patricia Simpson, Lindsie McCabe, Neil Cobb, and John Ascher</li></ul>

opencc-by-4.0Jan 2024View details →

◂Fig. 5 Gametogenesis in male and female Veneriserva pygoclava. A–D Semi-thin histological sections of female Veneriserva pygoclava, stained with toluidine blue. A Cross-section of a female Veneriserva. B Close-up of large mature oocytes without discernible nurse cells. C Developing oocytes attached to mesenteries (mes), and oogonia proliferating from the ventral side of the dorsal blood vessel (bv). D Details of vitellogenic oocytes and nurse cells. Arrowheads indicate brownstained yolk platelets and yolk bodies. E Live sperm cells captured in a light micrograph. F–G Cross-sections of male Veneriserva. Note the absence of a gut in the cross-sections. Abbreviations—ac acicula, acr acrosome, bv blood vessel, coe coelomic cavity, mes mesentery, nc nurse cell, nn nurse cell nucleus, nu sperm cell nucleus, Oo oocyte, on oocyte nucleus, sp spermatogonia, vnc ventral nerve cord in Hardly Venus's servant-morphological adaptations of Veneriserva to an endoparasitic lifestyle and its phylogenetic position within Dorvilleidae (Annelida)

◂Fig. 5 Gametogenesis in male and female Veneriserva pygoclava. A–D Semi-thin histological sections of female Veneriserva pygoclava, stained with toluidine blue. A Cross-section of a female Veneriserva. B Close-up of large mature oocytes without discernible nurse cells. C Developing oocytes attached to mesenteries (mes), and oogonia proliferating from the ventral side of the dorsal blood vessel (bv). D Details of vitellogenic oocytes and nurse cells. Arrowheads indicate brownstained yolk platelets and yolk bodies. E Live sperm cells captured in a light micrograph. F–G Cross-sections of male Veneriserva. Note the absence of a gut in the cross-sections. Abbreviations—ac acicula, acr acrosome, bv blood vessel, coe coelomic cavity, mes mesentery, nc nurse cell, nn nurse cell nucleus, nu sperm cell nucleus, Oo oocyte, on oocyte nucleus, sp spermatogonia, vnc ventral nerve cord

opencc-by-4.0Oct 2021View details →

◂Fig. 1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM788; b dorsal view of strain GeoM793; c apical view of strain GeoK044; d ventral view of strain GeoK037; e dorsal view of strain GeoM788; f apical view of strain GeoK024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK044; h leftlateral view of strain GeoM866; i motile cell of strain GeoK037; k–m coccoid cells showing variability in shape and size of strains k GeoM866, l GeoM793 and m GeoK024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV in Morphological and molecular variability of Peridinium volzii Lemmerm. (Peridiniaceae, Dinophyceae) and its relevance for infraspecific taxonomy

◂Fig. 1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV

opencc-by-4.0Oct 2015View details →

Text-fig. 4. Electrophoresis after amplification: Electrophoretical analysis of mitochondrial DNA. mtDNA sequences were amplified by primers F15.412 and R16.169 (450 bp), R16.269 (550 bp), R16.519 (800 bp). Lane 1 are primers F15.412 + R16.169, lane 2 primers F15.412 + R16.269, lane 3 primers F15.412 + R16.519, NC – negative control – water, L – 100 bp DNA ladder (band size from 100 bp to 1500 bp). in Genetic Analysis Of Possibly The Oldest Greyhound Remains Within The Territory Of The Czech Republic As Proof Of A Local Elite Presence At Chotěbuz-Podobora Hillfort In The 8 -9 Century Ad

Text-fig. 4. Electrophoresis after amplification: Electrophoretical analysis of mitochondrial DNA. mtDNA sequences were amplified by primers F15.412 and R16.169 (450 bp), R16.269 (550 bp), R16.519 (800 bp). Lane 1 are primers F15.412 + R16.169, lane 2 primers F15.412 + R16.269, lane 3 primers F15.412 + R16.519, NC – negative control – water, L – 100 bp DNA ladder (band size from 100 bp to 1500 bp).

opencc-zeroJan 2024View details →

Linked collectors and determiners for: Données d'occurrences RLA Flore NC.

Natural history specimen data linked to collectors and determiners held within, "Données d'occurrences RLA Flore NC". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="https://bionomia.net/dataset/19671673-994b-4100-89ed-dc4cc18e0adc">https://bionomia.net/dataset/19671673-994b-4100-89ed-dc4cc18e0adc</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/19671673-994b-4100-89ed-dc4cc18e0adc">https://gbif.org/dataset/19671673-994b-4100-89ed-dc4cc18e0adc</a>. Formatted as a Frictionless Data package.