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42 results for “Oscillatoriales”
FIGURE 2 in Description of a tropical new species of Wilmottia (Oscillatoriales, Cyanobacteria) and considerations about the monophyly of W. murrayi
FIGURE 2. Bayesian inference phylogenetic tree based on 16S rRNA gene sequences of oscillatorialean cyanobacteria. The Wilmottia clade, consisting of Brazilian (in bold), Antarctic and New Zealand strains, is highlighted. (a) Overview of topology. (b) Detail of Wilmottia clade with sub-clades A and B. Bootstrap (1000 resamplings) values (> 70%) and probabilities (> 0.7) obtained from ML/NJ/BI methods, respectively, are displayed at the relevant nodes. GenBank accession numbers are shown in parentheses. Bar: 0.05 substitutions per nucleotide position.
FIGURE 1 in Description of a tropical new species of Wilmottia (Oscillatoriales, Cyanobacteria) and considerations about the monophyly of W. murrayi
FIGURE 1. Brazilian Wilmottia strains. (a–l) W. murrayi. (a) 22PC; (b–c) 23PC; (d) 24PC; (e–f) 25PC; (g) 26PC; (h) 27PC; (i–j) 28PC; (k) 29PC; (l) 30PC. (m–o) W. stricta. (m) 16PC; (n–o) 31PC. Bars: 10 μm.
FIGURE 4 in Potamosiphon australiensis gen. nov., sp nov. (Oscillatoriales), a new filamentous cyanobacterium from subtropical north-eastern Australia
FIGURE 4. Phylogenetic tree based on the nifH gene sequence (312 bp) showing the phylogenetic position of Potamosiphon australiensis (shown in bold; scale bar = 0.05 nucleotide substitutions per site). Numbers indicate bootstrap values (>50%) from 1,000 replicates of neighbour-joining (NJ) and maximum likelihood (ML) analyses respectively. GenBank accession numbers are shown in parentheses.
FIGURE 1 in Potamosiphon australiensis gen. nov., sp nov. (Oscillatoriales), a new filamentous cyanobacterium from subtropical north-eastern Australia
FIGURE 1. Morphology of Potamosiphon australiensis (A–F) mature filaments with lamellated sheaths, (C–D) diagonal trichome fragmentation, (B, E, G) trichome fragmentation following formation of necridia; scale bar = 30 μm.
FIGURE 3 in Potamosiphon australiensis gen. nov., sp nov. (Oscillatoriales), a new filamentous cyanobacterium from subtropical north-eastern Australia
FIGURE 3. Phylogenetic tree based on the 16S rRNA gene sequence (1406–1412 bp) showing the phylogenetic position of Potamosiphon australiensis along with sequences from other taxa within the Oscillatoriales (scale bar = 0.02 nucleotide substitutions per site). Numbers indicate bootstrap values (>50%) from 1,000 replicates of neighbour-joining (NJ) and maximum likelihood (ML) analyses respectively. GenBank accession numbers are shown in parentheses.
FIGURE 5 in Potamosiphon australiensis gen. nov., sp nov. (Oscillatoriales), a new filamentous cyanobacterium from subtropical north-eastern Australia
FIGURE 5. Phylogenetic tree based on the 16S–23S rRNA operon (1971–2089 bp) showing the phylogenetic position of Potamosiphon australienses (shown in bold; scale bar = 0.02 nucleotide substitutions per site). Numbers indicate bootstrap values (>50%) from 1,000 replicates of neighbour-joining (NJ) and maximum likelihood (ML) analyses respectively. GenBank accession numbers are shown in parentheses.
FIGURE 2 in Potamosiphon australiensis gen. nov., sp nov. (Oscillatoriales), a new filamentous cyanobacterium from subtropical north-eastern Australia
FIGURE 2. Transmission electron micrographs of transverse (A) and longitudinal sections (B–C) of Potamosiphon australiensis showing location of thylakoids (th), cyanophycin granules (cy), cell wall (cw), and sheath (sh); scale bar = 5 μm.
FIGURE 5. Secondary structures for the D1–D1 in Morphology and molecular description of Wilmottia koreana sp. nov. (Oscillatoriales, Cyanobacteria) isolated from the Republic of Korea
FIGURE 5. Secondary structures for the D1–D1', Box-B, and V3 helix in the conserved regions of the 16S–23S internal transcribed spacer region: A, F, K,: Wilmottia murrayi KGI28; B, G, L: W. murrayi FBCC-A402; C, H, M: W. murrayi FBCC-A401; D, I, N, S: W. stricta 16PC; and E, J, O: W. koreana FBCC-A812. These structures were drawn considering Machado-de-Lima et al. (2017).
FIGURE 1 in Morphology and molecular description of Wilmottia koreana sp. nov. (Oscillatoriales, Cyanobacteria) isolated from the Republic of Korea
FIGURE 1. Microphotographs of Wilmottia koreana, from the reference strain FBCC-A812. (A–C) Fasciculated growth of filaments and (D–L) details of the trichomes and firm sheath (sh); Scale bars = A (100 µm), B and C (20 µm), D–L (10 µm).
FIGURE 4 in Morphology and molecular description of Wilmottia koreana sp. nov. (Oscillatoriales, Cyanobacteria) isolated from the Republic of Korea
FIGURE 4. Alignment of the 16S–23S rRNA internal transcribed spacer (ITS) region from Nostoc sp. and Wilmottia members. The different conserved domains (D1–D5), the tRNA genes, the major variable stems V2 and V3, and the antiterminator (Box B and Box A) are indicated by boxes and names. The Nostoc sequence contains both tRNAIle and tRNAAla, whereas all Wilmottia have only single tRNAIle. All the compared sequences contain all important structural elements of the ITS, as described by Boyer et al. (2001).
FIGURE 3 in Morphology and molecular description of Wilmottia koreana sp. nov. (Oscillatoriales, Cyanobacteria) isolated from the Republic of Korea
FIGURE 3. Maximum-likelihood (ML) tree of the phylogenetic relationships of species within the family Coleofasciculaceae (Geitlerinema, Potamolinea, Pycnacronema and Wilmottia) inferred from nearly complete 16S rRNA gene sequences, using Arthrospira platensis (CP013008) (Microcoleaceae) as the outgroup. Bayesian analysis was similar in topology of the ML tree and thus, its posterior probabilities (PP) are incorporated into the tree. Nodes display ML bootstrap support (> 50%) and Bayesian posterior probabilities (> 0.50), respectively. The branch lengths are proportional to the scale given.
FIGURE 2 in Morphology and molecular description of Wilmottia koreana sp. nov. (Oscillatoriales, Cyanobacteria) isolated from the Republic of Korea
FIGURE 2. Ultrastructure of two strains; (A–B) Wilmottia koreana strain FBCC-A812 (reference strain) and (C–D) W. murrayi strain FBCC-A401. (A–D) Parietal position of thylakoids (th), showing sheaths (sh) and cell wall (cw).
FIGURE 6 in Morphology and molecular description of Wilmottia koreana sp. nov. (Oscillatoriales, Cyanobacteria) isolated from the Republic of Korea
FIGURE 6. Nucleotide divergence of the Wilmottia 16S rRNA gene sequences (datasets used in Table 3) based on corrected p-distances. Genetic distances between each paired sequence were calculated by the Kimura 2-parameter model, where a total of 3 Wilmottia species (23 sequences) were compared. Bar heights indicate % p-distance measured for W. murrayi (Wm), W. stricta (Ws), and inter-species (e.g., W. koreana (Wk)). The values are shown as mean ±SD (Standard Deviation). Statistical analysis showed that the 16S rRNA gene divergences were significantly different between Wilmottia inter-species, but not intra-species (Student's t-test, ***P <0.01; N = 190 in Wm, 40 in Wm vs. Ws, 20 in Wm vs. Wk, and 2 in Wk vs. Ws respectively).
FIGURE 4. V3 helix. A. K. adunca ATA3-4Q in Polyphasic characterization of Kastovskya adunca gen. nov. et comb. nov. (Cyanobacteria: Oscillatoriales), from desert soils of the Atacama Desert, Chile
FIGURE 4. V3 helix. A. K. adunca ATA3-4Q-CV17, ATA3-5Q-CV5/LB5, B. Kastovskya adunca ATA6-11-RM4C/RM9/RM11, C. Kastovskya adunca ATA6-11-RM4A, D. Kastovskya adunca ATA6-11-RM10, E. Phormidium sp. B-Tom, F. Microcoleus sp. WJT32- NPBGF, G. Wilmottia murrayi.
FIGURE 3. D1-D1 in Polyphasic characterization of Kastovskya adunca gen. nov. et comb. nov. (Cyanobacteria: Oscillatoriales), from desert soils of the Atacama Desert, Chile
FIGURE 3. D1-D1' and Box-B helices. A–E. D1-D1' helix, F–I. Box B helix. A. Kastovskya adunca ATA3-4Q-CV17, ATA3-5Q- CV5/LB5, ATA6-11-RM4/RM9/RM11, B. Kastovskya adunca ATA6-11-RM10, C. Phormidium sp. B-Tom, D. "Microcoleus steenstrupii" WJT32-NPBGF, E. Wilmottia murrayi, F. Kastovskya adunca ATA3-4Q-CV17, ATA3-5Q-CV5/LB5, ATA6-11-RM4/ RM9/RM10/RM11, G. Phormidium sp. B-Tom, H. "Microcoleus steenstrupii" WJT32-NPBGF, I. Wilmottia murrayi.
FIGURE 2 in Polyphasic characterization of Kastovskya adunca gen. nov. et comb. nov. (Cyanobacteria: Oscillatoriales), from desert soils of the Atacama Desert, Chile
FIGURE 2. Phylogenetic position of the genus Kastovskya in the order Oscillatoriales based on Bayesian analysis with 16S rRNA gene sequence data. Posterior probabilities/bootstrap support from parsimony analysis reported above nodes.
FIGURE 1 in Polyphasic characterization of Kastovskya adunca gen. nov. et comb. nov. (Cyanobacteria: Oscillatoriales), from desert soils of the Atacama Desert, Chile
FIGURE 1. Morphological variability of Kastovskya adunca. A. Single filament of multiple intertwined trichomes. B–E. Various shapes of apical cells. F–G. Fasciculated thylakoids in cells. H. Necridia. I. Pointed apical cell. J. Hormogonium. K. Undulated edges of the sheath. L. Fasciculated thylakoids. M. Conical apical cell, sheath properties, and fascicles of thylakoids. Scale bar = 10 µm (A at lower magnification, B–M share the scale bar from B).
FIGURE 3 in New record of the rare genus Crinalium Crow (Oscillatoriales, Cyanobacteria) from sand dunes of the Baltic Sea, Germany: epitypification and emendation of Crinalium magnum Fritsch et John based on an integrative approach
FIGURE 3. Light micrographs showing an overview of living filaments of Crinalium magnum strain Hg-6-6. A, B. Irregular clusters with trichomes varying in length. C–E. Trichomes lying in two planes. F, G. Details of trichomes and terminal cells with a thickened outer margin. H–K. Fragmentation of trichomes in old cultures (6 and more months). Arrows mark the sheath. Scale bars: 10 µm
FIGURE 5 in New record of the rare genus Crinalium Crow (Oscillatoriales, Cyanobacteria) from sand dunes of the Baltic Sea, Germany: epitypification and emendation of Crinalium magnum Fritsch et John based on an integrative approach
FIGURE 5. Ultrastructure of C. magnum strain Hg-6-6. A. Longitudinal section of the trichome showing its general organization. B, D–F. Portions of filaments showing typical arrangement of helically twisted, swirl-like thylakoids and cell inclusions. C. Junctional pores (arrows) closely associated with the cross walls. Cx, carboxysomes; Cy, cyanophycin granules. Scale bars: 1 µm
FIGURE 4 in New record of the rare genus Crinalium Crow (Oscillatoriales, Cyanobacteria) from sand dunes of the Baltic Sea, Germany: epitypification and emendation of Crinalium magnum Fritsch et John based on an integrative approach
FIGURE 4. Staining of mucilage envelope of Crinalium magnum Hg-6-6. A–C. Staining with drawing ink showed difluent mucilage envelope. D–I. Staining with methylene blue showed striated structure of mucilage. E. Separate cells in lateral position with mucous microfibriles radiated from the cell wall. H, I Trichome in optical section (H) and in surface view (I) with increased portion showed mucous microfibrils arranged by rows along cross cell walls. Scale bars: 10 µm
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