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51 results for “PCR test”
Borderline COVID-19 PCR Test Result
ClinicalTrials.gov study NCT04636294. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Buccal Screening Evaluation Using an RT-PCR Assay and a Rapid ELISA Test Among Symptomatic and Asymptomatic Patients
ClinicalTrials.gov study NCT05074745. IPD Sharing: NO. Countries: 1. Publications: 1.
Self-Collected Saliva Samples Without Viral Transport Media for COVID-19 Testing Via RT-PCR
ClinicalTrials.gov study NCT04604145. IPD Sharing: Not stated. Countries: 1. Publications: 4.
Data from: An examination of the accuracy of a sequential PCR and sequencing test used to detect the incursion of an invasive species: the case of the red fox in Tasmania
1. Polymerase Chain Reaction (PCR) diagnostic tests are increasingly applied to the identification of wildlife. Yet rigorous verification is rare and the estimation of test accuracy (the probability that true positive and true negative samples are correctly identified – test sensitivity and specificity, respectively), particularly in combination with sequencing, is uncommon. This is important because PCR-based tests are prone to contamination in sampling and the laboratory. 2. Here, we use an experimental case–control approach to estimate the sensitivity and specificity of a sequential PCR-based wildlife detection test used to identify incursions of red foxes into Tasmania from predator faeces (scats). 3. Our results show that the sensitivity of the fox test is high (~94%) for the PCR-based test on its own, but this decreases to ~84% when combined with the DNA sequencing step. In contrast, the specificity increases from ~96% in the PCR only test to ~99.6% after inclusion of the DNA sequencing step. 4. The intense public scrutiny of the fox eradication program in Tasmania, has undoubtedly influenced the application of a sequential PCR test that maximises specificity at the expense of sensitivity and so increases the risk that scats containing fox DNA would not be detected. This could lead to the establishment of foxes in Tasmania as a consequence. 5. Synthesis and applications. Importantly, the estimation of the sensitivity and specificity of sequential tests enables decisions about the risk associated with mistaken identification (i.e. false negatives vs false positives) to be quantified for decision makers. The cost of false negative errors should be balanced against the costs of false positive errors, which could include the expenditure incurred in the application of unnecessary management actions were foxes not in fact present. Understanding the risks and costs associated with both false negative and false positive errors is therefore a key component to the decision making process for the management of the Tasmanian fox incursion.
Fig. 3 in Testing The Microsatellites-Pcr Markers For Genetic Diversity Research Of Alien Ponto-Caspian Amphipod Pontogammarus Robustoides G. O. Sars, 1894
Fig. 3. Microsatellites amplification of Pontogammarus robustoides from Pļaviņas Reservoir used primer Gapu-17.
FIGURE 1. A, B in A multiplex PCR method for identification of two common true cutworm species (Lepidoptera: Noctuidae) tested in the central plain of Guilan province, Iran
FIGURE 1. A, B, Maize and green pepper fields damaged by true cutworm.
Concordance between PCR-based extraction-free saliva and nasopharyngeal swabs for SARS-CoV-2 testing
<p><b>Introduction</b>: Saliva represents a less invasive alternative to nasopharyngeal swab (NPS) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection. SalivaDirect is a nucleic acid extraction-free method for detecting SARS-CoV2 in saliva specimens. <span>Studies evaluating the concordance of gold standard NPS and newly developed SalivaDirect protocols are limited</span>. The aim of our study was to assess SalivaDirect as an alternative method for COVID-19 testing.</p> <p><b>Methods</b>: Matching NPS and saliva samples were analysed from a cohort of symptomatic (n=127) and asymptomatic (n=181) participants recruited from hospital and university settings respectively. RNA was extracted from NPS while saliva samples were subjected to the SalivaDirect protocol before RT-qPCR analysis. The presence of SARS-Cov-2 was assessed using <i>RdRP</i> and <i>N1</i> gene targets in NPS and saliva respectively.</p> <p><b>Results</b>: Overall we observed 94.3% sensitivity (95% CI 87.2-97.5%), and 95.9% specificity (95% CI 92.4-97.8%) in saliva when compared to matching NPS samples. Analysis of concordance demonstrated 95.5% accuracy overall for the saliva test relative to NPS, and a very high level of agreement (κ coefficient = 0.889, 95% CI 0.833–0.946) between the two sets of specimens. Fourteen of 308 samples were discordant, all from symptomatic patients. Ct values were >30 in 13/14 and >35 in 6/14 samples. No significant difference was found in the Ct values of matching NPS and saliva sample (<i>p</i>=0.860). A highly significant correlation (r = 0.475, <i>p</i><0.0001) was also found between the Ct values of the concordant positive saliva and NPS specimens.</p> <p><b>Conclusions</b>: Use of saliva processed according to the SalivaDirect protocol represents a valid method to detect SARS-CoV-2. Less invasive and accurate saliva screening is an attractive alternative to current testing methods based on NPS and would afford greater capacity to test asymptomatic populations especially in the context of frequent testing.</p>
Figure 1 from: Hughes KW, Morris SD, Reboredo-Segovia A (2015) Cloning of ribosomal ITS PCR products creates frequent, non-random chimeric sequences – a test involving heterozygotes between Gymnopus dichrous taxa I and II. MycoKeys 10: 45-56. https://doi.org/10.3897/mycokeys.10.5126
Figure 1 - The ITS2 region of two haplotypes of Gymnopus dichrous: Haplotypes DI (represented by TENN68084) and DII (represented by TENN68078). The TC pair at position 15 and the indel at position 25 were used to determine which the haplotype was represented by the 5' end of a cloned sequence. Bases in red are points where DI and DII haplotypes differ in sequence and were used to determine if template switching had occurred in a cloned PCR product. Eight base pairs at which template switching can be detected are indicated by numbers 1-8. The possible area in which template switching (ts) could have occurred is indicated by vertical arrows and the number of observed template switching events is given above the vertical arrow. Bases that may be involved in intra-strand base pairing as determined by MFOLD are outlined with black boxes. Ambiguity codes indicate intraspecific variation.
Routine Application of Point-of-care PCR Test to Identify and Direct Therapy for Acute Respiratory Infection in the Emergency Department Trial
ClinicalTrials.gov study NCT06780566. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Clinical Utility of a PCR Compared to Culture and Sensitivity Testing for the Management of cUTI in Adults.
ClinicalTrials.gov study NCT06996301. IPD Sharing: NO. Countries: 1. Publications: 0.
The Clinical and Pharmacoeconomic Impact of Rapid Diagnostic Test (Multiplex PCR FilmArray) on Antimicrobial Decision Making Compared to Conventional Decision Making Among Critically Ill Patients
ClinicalTrials.gov study NCT05759494. IPD Sharing: UNDECIDED. Countries: 0. Publications: 4.
Clinical Evaluation of the Sentosa SA HSV1/2 Qualitative PCR Test
ClinicalTrials.gov study NCT02685956. IPD Sharing: NO. Countries: 1. Publications: 0.
Sex identification PCR-RFLP assay tested in eight species of Sebastes rockfish
Open the record for dataset details and reuse information.
Data from: An examination of the accuracy of a sequential PCR and sequencing test used to detect the incursion of an invasive species: the case of the red fox in Tasmania
Open the record for dataset details and reuse information.
Concordance between PCR-based extraction-free saliva and nasopharyngeal swabs for SARS-CoV-2 testing
Open the record for dataset details and reuse information.
Evaluation of Clinical Performance and IVD Test of LifeOS Digital PCR Liquid Biopsy Kits for EGFR T790M Mutation
ClinicalTrials.gov study NCT06573073. IPD Sharing: NO. Countries: 1. Publications: 0.
Performances of a H. Pylori Stool PCR Test
ClinicalTrials.gov study NCT03566004. IPD Sharing: NO. Countries: 1. Publications: 0.
This Trial is a Clinical Performance Validation Study That Will Evaluate the Clinical Agreement of the Sky Medical™ Rapid Antigen Test Comparing the Antigen Rapid Test to RT-PCR
ClinicalTrials.gov study NCT05491993. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Evaluation of Concordance Between Exhaled Air Test (eBAM-CoV) and RT-PCR to Detect SARS-CoV-2
ClinicalTrials.gov study NCT06099795. IPD Sharing: NO. Countries: 1. Publications: 0.
The Effect of Video Information on Anxiety in Adolescents Having rRT-PCR Test During Corona Virus Pandemic
ClinicalTrials.gov study NCT05265390. IPD Sharing: NO. Countries: 1. Publications: 0.
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Allen Brain Atlas
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.