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65 results for “Plant DNA barcoding”
FIGURE 10 in Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding
FIGURE 10. Mines, leaf shelters and pupation sites of Micrurapteryx and Caloptilia spp. from the Russian Far East. (A–B) Micrurapteryx caraganella, host plant Caragana arborescens; (C) M. gradatella, Vicia sp.; (D) Caloptilia acericola, Acer pseudosieboldianum; (E) C. alni, Alnus hirsuta; (F) C. betulicola, Betula platyphylla; (G) C. heringi, A. pictum; (H) C. stigmatella, Salix sp. Indications: (m) mine; (t) epidermal tunnel; (p) pupation site; (f) folded leaf margin or leaf tip; (r1–r2) rolled leaf margin or leaf tip (the indexes 1 and 2 indicate the order of construction appearance); *mine of Diptera. Close up: (C, G, H) mine. Sampling locations: (A–C) AO, Skovorodino, 26.VI.2016; (D) PK, Gornotaezhnoe, MTS and forest, 22–27.VII.2016; (E–H) SO, Sakhalin Isl., Yuzhno-Sakhalinsk, 11–20.VII.2017.
FIGURES 78–83 in Exploring the diversity of Gracillariidae (Lepidoptera) in South Africa: host plants, distribution, and DNA barcoding analysis, with the description of nine new species
FIGURES 78–83. Immature stages of South African Gracillariidae. 78, Phodoryctis tephrosiella, mines on Tephrosia rhodesica (Fabaceae). Gauteng, Tshwane, A. Sharp leg. 79, Conopobathra carbunculata, mines on Peltophorum africanum (Fabaceae), Limpopo, York, A. & I. Sharp leg. 80, Phyllonorycter grewiella, mine on Grewia hexamita (Malvaceae), A. & I. Sharp leg. 81, P. pseudogrewiella sp. nov., mines on Grewia flavescens (Malvaceae), Limpopo, Hoedspruit, A. & I. Sharp leg. 82, P. pseudogrewiella sp. nov., larva, ibidem. 83, P. pseudogrewiella sp. nov., mines and pupae, ibidem.
FIGURES 67–72 in Exploring the diversity of Gracillariidae (Lepidoptera) in South Africa: host plants, distribution, and DNA barcoding analysis, with the description of nine new species
FIGURES 67–72. Immature stages of South African Gracillariidae. 67, Conopomorphina aptata, mine on Schotia brachypetala (Fabaceae), Limpopo, Hoedspruit, A. & I. Sharp leg. 68, Acrocercops syzygiena, mine on Syzygium cordatum (Myrtaceae), Gauteng, Tshwane, A. & I. Sharp leg. 69, A. syzygiena, larva, ibidem. 70, A. combreticola, mine on Combretum zeyheri (Combretaceae) Gauteng, Tshwane, A. Sharp leg. 71, Cryptolectica capnodecta, larva on Syzygium cordatum (Myrtaceae), Limpopo, Hoedspruit, A. & I. Sharp leg. 72, C. terminalina, mine on Terminalia sericea (Combretaceae), A. & I. Sharp leg.
FIGURES 84−88 in Exploring the diversity of Gracillariidae (Lepidoptera) in South Africa: host plants, distribution, and DNA barcoding analysis, with the description of nine new species
FIGURES 84−88. Immature stages of South African Gracillariidae species. 84, Cameraria melhaniella sp. nov., larva on Melhania acuminata (Malvaceae), Limpopo, Hoedspruit, A. & I. Sharp leg. 85, Metriochroa pergulariae, mine on Pergularia daemia (Apocynaceae), Limpopo, Hoedspruit, A. & I. Sharp leg. 86, Phyllocnistis allisonae, mine on Protea rubropilosa (Proteaceae), Limpopo, Lopez Vaamonde leg. 87, Phyllocnistis magalismontani sp. nov., mine on Englerophytum magalismontanum (Sapotaceae), Limpopo, Hoedspruit, leg. A. & I. Sharp. 88, Phyllocnistis faureae, mine on Faurea saligna (Proteaceae), Gauteng, Tshwane, leg. A. Sharp.
FIGURES 61−66 in Exploring the diversity of Gracillariidae (Lepidoptera) in South Africa: host plants, distribution, and DNA barcoding analysis, with the description of nine new species
FIGURES 61−66. Immature stages of South African Gracillariidae. 61, Caloptilia sp., larva on Cryptocarya transvaalensis (Lauraceae), Limpopo, Lekgalameetse N. R., A. & I. Sharp leg. 62, C. cataractias, larva on Rhynchosia minima (Fabaceae), Limpopo, Hoedspruit, A. & I. Sharp leg. 63, Macarostola noellineae, larva on Syzygium cordatum (Myrtaceae), Limpopo, Hoedspruit, A. & I. Sharp leg. 64, Ectropina spirostachydis sp. nov., larva on Spirostachys africana (Euphorbiaceae), Limpopo, Hoedspruit, A. & I. Sharp leg. 65, Cuphodes melanostola, mine on Euclea divinorum (Ebenaceae), Limpopo, Hoedspruit, A. & I. Sharp leg. 66, Conopomorphina ochnivora, Gauteng, Magaliesburg, on Ochna pretoriensis, (Ochnaceae), H. Staude leg.
FIGURES 58–60 in Exploring the diversity of Gracillariidae (Lepidoptera) in South Africa: host plants, distribution, and DNA barcoding analysis, with the description of nine new species
FIGURES 58–60. Female genitalia of South African Gracillariidae. 58-59, Phyllocnistis magalismontani sp. nov., paratype: 58, lateral view; 59, signa. 60, P. allisonae sp. nov., holotype: ventral view. (All scale bar 190 μm).
FIGURES 56–57 in Exploring the diversity of Gracillariidae (Lepidoptera) in South Africa: host plants, distribution, and DNA barcoding analysis, with the description of nine new species
FIGURES 56–57. Female genitalia of South African Gracillariidae. 56, Telamoptilia cordati sp. nov., paratype: ventral view. 57, Phyllonorycter pseudogrewiella sp. nov., holotype: lateral view. (All scale bar 190 μm).
FIGURES 44–46 in Exploring the diversity of Gracillariidae (Lepidoptera) in South Africa: host plants, distribution, and DNA barcoding analysis, with the description of nine new species
FIGURES 44–46. Male genitalia of South African Gracillariidae. Phodoryctis tephrosiella sp. nov.: 44, holotype, ventral view; 45, phallus with bulbus ejaculatorius; 46, segment VIII in ventral view (ae: aedeagus; pb: phallobase; be: bulbus ejaculatorius). (All scale bars 190 μm).
FIGURES 25−30 in Exploring the diversity of Gracillariidae (Lepidoptera) in South Africa: host plants, distribution, and DNA barcoding analysis, with the description of nine new species
FIGURES 25−30. Forewing pattern of South African Gracillariidae. 25, Leucocercops curatellifoliae sp. nov., Limpopo. 26, Phodoryctis tephrosiella sp. nov., Gauteng. 27, Telamoptilia cordati sp. nov., South Africa, Limpopo. 28, Telamoptilia sp., Madagascar. 29, Cameraria melhaniella sp. nov., Limpopo. 30, Phyllonorycter pseudogrewiella sp. nov., Limpopo.
FIGURES 13–18 in Exploring the diversity of Gracillariidae (Lepidoptera) in South Africa: host plants, distribution, and DNA barcoding analysis, with the description of nine new species
FIGURES 13–18. Adults of South African Gracillariidae. 13, Cryptolectica capnodecta, Limpopo, Hoedspruit, A. & I. Sharp leg. 14, C. terminalina, Limpopo, Hoedspruit, A. & I. Sharp leg. 15, Dialectica pyramidota, Limpopo, Hoedspruit, A. & I. Sharp leg. 16, Amblyptila cynanchi, Western Cape, Knysna, S. Mecenero leg. 17, Leucocercops dasmophora, Gauteng, Tshwane, A. & I. Sharp leg. 18, L. curatellifoliae sp. nov. Limpopo, Hoedspruit, A. & I. Sharp leg.
FIGURES 32–34 in Exploring the diversity of Gracillariidae (Lepidoptera) in South Africa: host plants, distribution, and DNA barcoding analysis, with the description of nine new species
FIGURES 32–34. Forewing pattern of the South African Phyllocnistinae. 32, Phyllocnistis magalismontani sp. nov., drawn by holotype; 33, P. allisonae sp. nov., holotype; 34, P. faureae, holotype.
FIGURE 31 in Exploring the diversity of Gracillariidae (Lepidoptera) in South Africa: host plants, distribution, and DNA barcoding analysis, with the description of nine new species
FIGURE 31. Forewing pattern of the Afrotropical Phyllocnistinae. A, Phyllocnistis pharetrucha; B, P. saligna; C, P. magalismontani sp. nov.; D, P. faureae sp. nov.; E, P. loxosticha; F, P. citrella; G, P. allisonae sp. nov..
FIGURES 1−6 in Exploring the diversity of Gracillariidae (Lepidoptera) in South Africa: host plants, distribution, and DNA barcoding analysis, with the description of nine new species
FIGURES 1−6. Adults of South African Gracillariidae. 1, Caloptilia sp., Limpopo, Lekgalameetse N. R., A. & I. Sharp leg. 2, Caloptilia rhusina, Western Cape, Robberg Nature Reserve, S. Mecenero leg. 3, Macarostola noellineae, Limpopo, Hoedspruit, A. & I. Sharp leg. 4, Caloptilia cataractias, Limpopo, Hoedspruit, leg. A. & I. Sharp. 5, Ectropina spirostachydis sp. nov., Limpopo, Hoedspruit, A. & I. Sharp leg. 6, Graphiocephala barbitias, Gauteng, Tshwane, A. Sharp leg.
FIGURES 7–12 in Exploring the diversity of Gracillariidae (Lepidoptera) in South Africa: host plants, distribution, and DNA barcoding analysis, with the description of nine new species
FIGURES 7–12. Adults of South African Gracillariidae. 7, Semnocera procellaris, Limpopo, Hoedspruit, A. & I. Sharp leg. 8, Conopomorphina ochnivora, Gauteng, Magaliesburg, A. & I. Sharp leg. 9, C. aptata, Limpopo, Hoedspruit, A. & I. Sharp leg. 10, Cuphodes melanostola, Limpopo, Hoedspruit, A. & I. Sharp leg. 11, Acrocercops syzygiena, Gauteng, Tshwane, A. Sharp leg. 12, A. combreticola, Gauteng, Tshwane, A. Sharp leg.
Data from: Using a comprehensive DNA barcode library to detect novel egg and larval host plant associations in a Cephaloleia Rolled-leaf Beetle (Coleoptera: Chrysomelidae)
To fully understand the ecology and evolution of plant-herbivore interactions, information regarding the life history of both immature and adult insect stages is essential. However, most knowledge of plant-herbivore associations is derived from observations of adults. One reason for this bias is that species identification of immature stages is usually challenging. DNA barcodes can be used to identify immature stages to the species-level. This technique compares short sequences of the appropriate DNA barcode loci (e.g., mitochondrial COI gene for insects) of an unidentified specimen to a known DNA barcode library. The accuracy of DNA-based identifications depends on the comprehensiveness of the DNA barcode library. We generated a comprehensive DNA barcode library for a community of Rolled-leaf Beetles (Coleoptera: Chrysomelidae) in a premontane tropical forest in Costa Rica. The DNA barcode COI accurately identified all beetle species included in this study. Using this DNA barcode library, we identified eggs and larvae of Cephaloleia histrionica Baly with 100% confidence. This new record of C. histrionica is unique in that this species completes its life cycle on a bromeliad, whereas most Cephaloleia species are associated with plants from the order Zingiberales. The life cycle, diet breadth, immature stages, and sexual dimorphism are described for C. histrionica.
Data from: Seeing is believing? comparing plant-herbivore networks constructed by field co-occurrence and DNA barcoding methods for gaining insights into network structures
Plant-herbivore interaction networks provide information about community organization. Two methods are currently used to document pairwise interactions among plants and insect herbivores. One is the traditional method that collects plant-herbivore interaction data by field observation of insect occurrence on host plants. The other is the increasing application of newly developed molecular techniques based on DNA barcodes to the analysis of gut contents. The second method is more appealing because it documents realized interactions. To construct complete networks, each technique of network construction is urgent to be assessed. We addressed this question by comparing the effectiveness and reliability of the two methods in constructing plant-Lepidoptera larval network in a 50 ha subtropical forest in China. Our results showed that the accuracy of diet identification by observation method increased with the number of observed insect occurrences on food plants. In contrast, the molecular method using three plant DNA markers were able to identify food residues for 35.6% larvae and correctly resolved 77.3% plant (diet) species. Network analysis showed molecular networks had three-fold more unique host plant species but fewer links than the traditional networks had. The molecular method detected plants that were not sampled by the traditional method, e.g., bamboos, bryophytes and lianas in the diets of insect herbivores. The two networks also possessed significantly different structural properties. Our study indicates the traditional observation of co-occurrence is inadequate, while molecular method can provide higher species resolution of ecological interactions.
FIGURES 24–30 in New record of Epermenia (Calotripis) sinjovi Gaedike (Lepidoptera: Epermeniidae) in China: DNA barcode, adult, immature stages, host plant and biology
FIGURES 24–30 Pupa of Epermenia (Calotripis) sinjovi 24, ventral view; 25, dorsal view; 26, lateral view; 27, subcircular hollow surrounded by two crescent sclerotizations; 28, genitalia orifice and anus, male; 29, genitalia orifices and anus, female; 30, curved and hooked setae on cremaster.
FIGURES 2–10 in New record of Epermenia (Calotripis) sinjovi Gaedike (Lepidoptera: Epermeniidae) in China: DNA barcode, adult, immature stages, host plant and biology
FIGURES 2–10. Adults and wing venations of Epermenia (Calotripis) sinjovi 2–3, normal phenotype, male; 4, brown phe-
FIGURES 11–19 in New record of Epermenia (Calotripis) sinjovi Gaedike (Lepidoptera: Epermeniidae) in China: DNA barcode, adult, immature stages, host plant and biology
FIGURES 11–19. Genitalia and pockets of Epermenia (Calotripis) sinjovi 11 and 13, male genitalia and aedeagus, genitalia slide no. WYH0013; 12, valva with shorter cucullus, genitalia slide no. WYH0017; 14–15, aedeagus of variable lengths, 15, vesica protruded, length of aedeagus referring to the distance between blue dots, that of cornutus between red dots, genitalia slide nos. 14, WYH0011, 15, WYH0017; 16, pockets with androconial scales, slide no. WYH0009; 17, female genitalia, WYH0014; 18, coniform granules in ductus bursae; 18, signum with teeth along edge.
FIGURES 20–23 in New record of Epermenia (Calotripis) sinjovi Gaedike (Lepidoptera: Epermeniidae) in China: DNA barcode, adult, immature stages, host plant and biology
FIGURES 20–23 Larva and biology of Epermenia (Calotripis) sinjovi 20, larva; 21–23, mines, arrows indicating early small mines.
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