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24 results for “Scanning Electron Microscopy (SEM)”
Scanning electron microscopy (SEM) images of single bacterial-Pb tolerant isolates
<p><strong>In-Vitro Trace Element Uptake by Bacteria in Liquid Cultures-Scanning Electron Microscopy </strong><strong>(SEM-EDX) Analysis</strong><br>Batch studies were conducted using 50 mL Falcon tubes that contained 25 mL GY rich medium supplemented with 100 mg L-1 of Pb (Pb(NO3)2). All tubes were inoculated with 1 mL of a bacterial cell suspension (optical density at 600 nm = 1), which was previously grown in GY medium at 28 °C for 24 h. Samples were incubated at 28 °C and 100 rpm on an orbital shaker.<br>In order to estimate Pb sequestration by bacterial cells, cultures were first centrifuged 15 min at 4800 rpm, bacterial pellets were washed 3 times with 0.01 M phosphate-buffer (PBS, pH 7.0) to remove unbound metals, sugars and proteins. The samples were dehydrated with ethanol/water mixtures, until absolute ethanol. Then 100% acetone was used. Pellets were resuspended in 2% glutaraldehyde for 1h at room temperature. Afterwards, bacterial samples were centrifuged for 3 min at 3000 rpm and pellets were washed 3 times with milli-Q water. One uL of sample was placed on a sample holder, in carbon conductive tape. Then, samples were coated 30 s with a 15 nm gold layer and analysed using a Scanning Electron Microscope (<span>Phenom™ ProX Desktop, Thermofisher</span>). Images were taken using an accelerating voltage of 15 kV.</p> <p><em>Bacillus paramycoides</em> ST4, <em>Bacillus wiedmannii</em> ST29 and <em>Bacillus proteolyticus </em>ST9 <span>images obtained by SEM-EDX are presented in this dataset. Pb was found present in/on the bacterial cell wall of these bacteria cultures and detected by EDX spectra. </span></p>
Scanning electron microscopy (SEM) images of particulate matter collected on air filters
<p>Airborne PM sampling was conducted within a larger study on the PM composition of different areas in Santa Rosa, La Pampa, Argentina by Prof. Dr. Mendez Mariano. Airborne PM10 samples were collected on commercial 47mm diameter PTFE membrane filters (Image 1-blank) and Nylon filters (Image 2-blank). The PM10 was collected using an electrostatic precipitator coupled with the Easy Dust Generator (EDG). Filters were analysed using a Scanning Electron Microscope (Phenom™ ProX Desktop, Thermofisher). Images were taken using an accelerating voltage of 15 kV. SEM-EDX results are presented in this dataset.</p>
SEM imaging data used in "Investigation of the porosity of L/LL4 ordinary chondrite Bjurböle using synchrotron radiation microtomography and scanning electron microscopy: Implications for parent body evolution"
<p>SEM imaging data used in ”Investigation of the porosity of L/LL4 ordinary chondrite Bjurböle using synchrotron radiation microtomography and scanning electron microscopy: Implications for parent body evolution” contains images of a polished section of a 0.35 cm<sup>3</sup> sample of Bjurböle obtained using scanning electron microscopy (SEM) in backscattered electron mode (pixel size 0.55 µm), as well as elemental maps of some details of the sample obtained by an energy dispersive spectrometer, as zip archives. Folder SEM contains the images covering the entire polished section. Bulk porosity of the sample was determined to be 21.9 vol% using a gas pycnometer. </p>
Text-fig. 35. Scanning electron microscope (SEM, a, b, d) and synchrotron radiation X-ray tomographic microscopy (SRXTM, c, e) images of "Paisia-like follicle"; Catefica locality, Portugal. a) Lateral view of slender follicle with an almost straight ventral margin and a slightly curved dorsal margin; b) Lateral view of small, broad follicle with slightly sinuous ventral margin and rounded dorsal margin; c) Lateral view of small, broad follicle with slightly sinuous ventral margin and rounded dorsal margin; d) Apical part of follicle in (a) showing the slight apical cleft in the probable stigmatic region; e) Transverse section (orthoslice xy0407) of follicle showing ovules and distinct follicle wall with small, thin-walled cells of the outer epidermis (arrow), larger, isodiametric cells of the mesocarp and an inner layer of smaller, thin-walled cells. Specimens, Catefica 50-S171523 (a, d), Catefica 343-S171515 (b), Catefica 49-S174929 (c, e). Scale bars = 300 Μm (a–c), 100 Μm (d, e). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 35. Scanning electron microscope (SEM, a, b, d) and synchrotron radiation X-ray tomographic microscopy (SRXTM, c, e) images of "Paisia-like follicle"; Catefica locality, Portugal. a) Lateral view of slender follicle with an almost straight ventral margin and a slightly curved dorsal margin; b) Lateral view of small, broad follicle with slightly sinuous ventral margin and rounded dorsal margin; c) Lateral view of small, broad follicle with slightly sinuous ventral margin and rounded dorsal margin; d) Apical part of follicle in (a) showing the slight apical cleft in the probable stigmatic region; e) Transverse section (orthoslice xy0407) of follicle showing ovules and distinct follicle wall with small, thin-walled cells of the outer epidermis (arrow), larger, isodiametric cells of the mesocarp and an inner layer of smaller, thin-walled cells. Specimens, Catefica 50-S171523 (a, d), Catefica 343-S171515 (b), Catefica 49-S174929 (c, e). Scale bars = 300 Μm (a–c), 100 Μm (d, e).
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