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2,318 results for “Synthesis”
ZooBase: A global synthesis of marine zooplankton species occurrences.
<p><em><strong>Description of the methods used to implement the present ZooBase dataset (extarct from Section A.2 from the Methods of Benedetti et al., 2021).</strong></em></p> <p>A new dataset of global zooplankton species occurrences was compiled in a comparable fashion to that put together for phytoplankton (Righetti et al., 2020). Prior to retrieving the occurrence data online, we first identified the phyla (Order/Class/Family) that comprise the bulk of extant oceanic zooplankton communities: Copelata (i.e. appendicularians), Ctenophora, Cubozoa (i.e. box jellyfish), Euphausiidae (i.e. krill), Foraminifera, Gymnosomata (i.e. sea angels, pteropods), Hydrozoa (i.e. jellyfish), Hyperiidea (i.e. amphipods), Myodocopina (i.e. ostracods), Mysidae (i.e. small pelagic shrimps resembling krill), Neocopepoda, Podonidae and <em>Penilia</em> <em>avirostris</em> (i.e. cladocerans), Sagittoidea (i.e. chaetognaths), Scyphozoa (i.e. jellyfish), Thaliacea (i.e. salps, doliolids and pyrosomes), Thecosomata (i.e. pteropods), and four families of pelagic Polychaeta (i.e. worms) that are often found in the zooplankton and whose species are known to display holoplanktonic lifecycles (Tomopteridae, Alciopidae, Lopadorrhynchidae, Typhloscolecidae). The presence data associated with species belonging to these groups were retrieved from OBIS and GBIF between the 12/04/2018 and the 18/04/2018 using online queries via the R packages RPostgreSQL, robis and rgbif. Since the Neocopepoda infra-class comprise several thousands of benthic and parasitic taxa (https://copepodes.obs-banyuls.fr/en/), a preliminary selection of the non-parasitic planktonic species had to be carried out prior to the online downloading using the species list of Razouls et al. (https://copepodes.obs-banyuls.fr/en/) as a reference. The spatial distributions of the groups cited above were first inspected using GBIF’s and OBIS’s online mapping tools to evaluate the potential number of overlapping observations between the two databases. As a result of their relatively low contributions to total observations/diversity, and very high overlap between databases, the occurrences of Cladocera and Polychaeta were retrieved from OBIS only (which usually harbours more occurrences). On top of the data collected from OBIS and GBIF, the copepod occurrences from Cornils et al. (2018) and the pteropod occurrences from the MAREDAT initiative (Buitenhuis et al., 2013) were added to the dataset. We discarded records that: (i) presented at least one missing spatial coordinate, (ii) were associated with an incomplete sampling date (d/m/y), (iii) were associated with a year of collection older than 1800, (iv) were not associated with any sampling depth, (v) were not identified down to the species level. Occurrences associated with grid cells shallower than 10m were removed (bathymetry data from ETOPOv1 at a 15min resolution, downloaded using the 'marmap' R package). Finally, every species name was then carefully examined and compared to the taxonomic reference list of the World Register of Marine Species (WoRMS; <a href="http://www.marinespecies.org">http://www.marinespecies.org</a>) for all taxa. The AphiaID and the Status were retreived from WoRMS based on the ScientificName. To remove the duplicate occurrences due to the highly overlapping source archives (GBIF and OBIS), a unique occurrenceID was given to each record based on rounded spatial coordinates (closest 0.1°x0.1°), rounded depth layer (10m depth layers), month and year of the occurrence and the acccpted species name (e.g., AphiaID).</p> <p><strong>This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 862923. This output reflects only the author’s view, and the European Union cannot be held responsible for any use that may be made of the information contained therein.</strong></p>
Environmental and economic potential of decentralised electrocatalytic ammonia synthesis powered by solar energy
<p>Dataset associated with the publication "Environmental and economic potential of decentralised electrocatalytic ammonia synthesis powered by solar energy" by Sebastiano C. D'Angelo, Antonio J. Martín, Selene Cobo, Diego Freire-Ordóñez, Gonzalo Guillén-Gosálbez, and Javier Pérez-Ramírez, available at <a href="https://doi.org/10.1039/D2EE02683J">https://doi.org/10.1039/D2EE02683J</a>. The dataset includes the numeric data required to plot all the figures embedded in the main manuscript and in the Electronic Supplementary Information (ESI).</p> <p>The structure of the dataset is here elucidated sheet by sheet:</p> <ul> <li><strong>GeneralParameters</strong>: numerical values for the scaled functional unit used in the study, the world population value adopted, and the three voltage efficiencies assumed in different parts of the study.</li> <li><strong>AL_BaseCase_SensECE</strong>: numerical values associated with the results for the ammonia leaf scenarios adopting a voltage efficiency of 63% (base case) and a Faradaic efficiency varying from 1% to 100%; highest, average, and lowest capacity factors for the solar power production were here used. The ammonia leaf configuration here assessed is the one including solar panels, electrolyzer, and fuel cell as key components. The results report all the ReCiPe 2016 (hierarchical approach) midpoints and endpoints and the values for the assessed planetary boundaries; the levelised cost of ammonia (LCOA) is reported, as well.</li> <li><strong>AL_EtaV75_SensECE</strong>: this sheet has the structure as the previous one, but includes the results for the ammonia leaf scenario using 75% voltage efficiency, instead of 63%. The remaining assumptions do not deviate from the base case.</li> <li><strong>AL_Eta100_SensECE</strong>: this sheet has the structure as the previous one, but includes the results for the ammonia leaf scenario using 100% voltage efficiency, instead of 63%. The remaining assumptions do not deviate from the base case.</li> <li><strong>AL_NoFC_H2Vented_SensECE</strong>: this sheet has the same structure as the sheet "AL_BaseCase_SensECE", but includes the ammonia leaf scenario using a configuration with no fuel cell. The hydrogen by-product was here considered vented to the air. The remaining assumptions do not deviate from the base case.</li> <li><strong>AL_NoFC_H2Subst_SensECE</strong>: this sheet has the same structure as the sheet "AL_BaseCase_SensECE", but includes the ammonia leaf scenario using a configuration with no fuel cell. The hydrogen by-product was here considered substituting the production of an equivalent quantity from a water electrolyzer deployed in the same location as the ammonia leaf. The remaining assumptions do not deviate from the base case.</li> <li><strong>AL_BaseCase_SpatAnal_BreakFEff</strong>: numerical results for the ammonia leaf base case scenario stemming from the spatial analysis performed on a global grid of 1140 points. The yearly average capacity factors for the solar panels at each location are included, and the results portraying the breakeven Faradaic efficiency for the indicators climate change - CO<sub>2</sub> concentration, global warming, human health, and levelised cost of ammonia were included. The assumptions for the voltage efficiency and the other parameters correspond to the base case.</li> <li><strong>AL_BaseCase_SpatAnal_AbsValues</strong>: numerical results for the ammonia leaf scenarios using the base case state-of-the-art (34%) and 100% Faradaic efficiency, as well as the base case voltage efficiency of 63%. The same metrics as the previous sheet are reported. The structure of the sheet is the same as the previous one.</li> <li><strong>AL_BaseCase_Breakdowns</strong>: breakdown of the same four indicators as the previous sheet for the best and worst combination of Faradaic efficiency and solar panels capacity factors, i.e., 34% Faradaic efficiency and 6% capacity factor on one side and 100% Faradaic efficiency and 26% capacity factor on the other side. The breakdown is divided into solar panels, electrolyser, fuel cell, and other elements. A further breakdown of the levelised cost of ammonia (LCOA) into capital expenditure (CAPEX) and operating expenditure (OPEX) is provided, as well. The voltage efficiency is the same as the base case, as well as the other parameters.</li> <li><strong>AL_BaseCase_CAPEXSens</strong>: numerical results for the levelised cost of ammonia (LCOA) in dependence of the sensitivity on the capital expenditure (CAPEX) for the ammonia leaf configuration assessed in the base case. Two cases assuming state-of-the-art (34%) and 100% Faradaic efficiency were assumed, and lowest, average, and highest capacity factor are included. The remaining parameters do not deviate from the base case configuration.</li> <li><strong>AL_gHB_BestMap</strong>: numerical results to produce the map showing the best technology between ammonia leaf (AL) and green Haber-Bosch (gHB) in the category climate change - CO<sub>2</sub> concentration for all the assessed locations. column D shows the share of safe operating space (%SOS) for each location, while column E shows which technology was selected, where 1 is ammonia leaf and 2 is green HB.</li> <li><strong>AL_BaseCase_Sensitivity</strong>: percentual variation of the results obtained assuming the base configuration ammonia leaf for a state-of-the-art Faradaic efficiency and an average capacity factor for the solar panels. The varied parameters include the voltage efficiency (columns C-D-E), the levelised cost of electricity (columns G-H-I), the electrolyser cost (columns K-L-M), the fuel cell cost (columns O-P-Q), the electrolyser environmental impact (columns S-T-U), and the fuel cell environmental impact (columns W-X-Y).</li> <li><strong>CompTech_BaseCase</strong>: environmental and economic metrics characterizing the assessed Haber-Bosch scenarios (business as usual, BAU; blue Haber-Bosch; green Haber-Bosch for lowest, average, and highest solar panels capacity factor; BAU assuming natural gas spot prices in Europe in August 2022). The reported metrics are the ReCiPe 2016 (hierarchical approach) midpoints and endpoints, the planetary boundaries, and the levelised cost of ammonia (LCOA).</li> <li><strong>CompTech_EtaV75</strong>: this sheet has the same structure as the previous one, but the hydrogen electrolyser used for the green Haber-Bosch scenarios was assumed to have a 10% stack efficiency improvement. The remaining parameters are the same.</li> <li><strong>CompTech_EtaV100</strong>: this sheet has the same structure as the previous one, but the hydrogen electrolyser used for the green Haber-Bosch scenarios was assumed to have a 100% stack efficiency. The remaining parameters are the same.</li> <li><strong>CompValues_Fig1</strong>: numerical values for yearly global warming impacts of a selection of countries, as well as for the yearly human health impacts of selected diseases and catastrophic events.</li> </ul> <p> </p>
Data set for the journal article: Colloidal-ALD Grown Metal Oxide Shells Enable the Synthesis of Photoactive Ligand/ Nanocrystal Composite Materials
<p>The data for each figure of the main manuscript is included in this folder.</p> <p>Figure 1 is not included as it contains no data.</p> <p>The folder for Figure 2 contains a sub-folder for the EDX and NMR data of 9-ACA/PbS@AlOx. The NMR data was processed by Mestrenova.</p> <p>The folder for Figure 3 contains optical absorption spectrum data of 9-ACA/PbS@AlOx.</p> <p>The folder for Figure 4 contains NMR data which was processed by Mestrenova. It contains the data for 9-ACA/CuInS2@AlOx, 1-PCA/CsPbBr3@AlOx and 9-PTA/CsPbBr3@AlOx.</p> <p>The folder for Figure 5 is made of three sub-folders for figure 5A, 5B and 5C. 5A and 5B contain optical absorption for the CuInS2 and CsPbBr3 datasets while 5C contain time resolved data for CsPbBr3.</p> <p>The folder for Figure 6 contains time resolved PL for the as synthesized CsPbBr3, 1-PCA/CsPbBr3@AlOx and 9-PTA/CsPbBr3@AlOx. The 9-PTA/CsPbBr3@AlOx data contain two decays that span 200 ns (short) or 13.5 us (long).</p> <p>The folder for Figure 7 contains time resolved PL for the as synthesized 9-PTA/CsPbBr3@AlOx and 1-PCA/9-PTA/CsPbBr3@AlOx. For both samples the data contain two decays that span 200 ns (short) or 13.5 us (long). Also an NMR folder is present with the 1H spectrum for 9-PTA/CsPbBr3@AlOx and 1-PCA/9-PTA/CsPbBr3@AlOx.</p> <p> </p> <p> </p>
Hydrodynamic modeling data for Synthesis of Juvenile Steelhead Responses to Hydrodynamic Conditions in the Sacramento-San Joaquin Delta
This data release includes the output variables extracted from the UnTRIM Bay-Delta hydrodynamic model (hydrodynamic model) for use in evaluating the effects of hydrodynamics on the behavior of acoustically-tagged juvenile steelhead in the Sacramento-San Joaquin Delta. Work was funded by Proposition 1 and completed by Anchor QEA, LLC, and U.S. Geological Survey for the State Water Contractors under a Proposition 1 Grant (Evaluating Juvenile Salmonid Behavioral Responses to Hydrodynamic Conditions in the Sacramento-San Joaquin Delta), contracted by Delta Stewardship Council. Not all the hydrodynamic model output variables in the output provided with this memorandum were used in the final fish models used to analyze steelhead responses. Model output for additional variables and locations were included for completeness and to make these output files more broadly useful to researchers interested in other locations or variables in the Sacramento-San Joaquin Delta. Hydrodynamic model simulations were conducted for 2011, 2012, 2013, 2014, 2015, and 2016, with hydrodynamic model output variables provided at the same locations for each period simulated. The model simulation for each year spanned the full period of steelhead detections in the telemetry data collected during that year.
Interagency Ecological Program: Data for Synthesis for Ecological Impacts of Drought in the Upper San Francisco Estuary, 1975-2021
These data were collected for the special issue on Drought in the Sacramento-San Joaquin Delta published in San Francisco Estuary and Watershed Sciences, Volume 22, Issue 1, March of 2024. Multi-year droughts are important and impactful features of California’s Mediterranean climate and can fundamentally affect the water quality and the ecosystem response of the San Francisco Estuary and the Sacramento-San Joaquin Delta. This data set was assembled from data collected by long-term monitoring programs over the past 46 years (1975-2021) to evaluate how the estuary changed during multi-year droughts. Data include fish, zooplankton, jellyfish, clams, temperature, Secchi depth, and salinity as measured by fish and water quality surveys, as well as nutrients and chlorophyll measured by water quality surveys. These data are accompanied by hydrologic metrics including Delta Inflow, Delta Outflow, the position of the 2-PSU isohaline, residence time, and daily values of various continuous water quality stations located in the Delta.
FIGURE 1 from conference paper AIP Proceedings 2145 "Solid state synthesis of CdS quantum dots through laser direct writing"
<p>FIGURE 1 of the conference paper "Solid state synthesis of CdS quantum dots through laser direct writing"</p>
FIGURE 4 from conference paper AIP Proceedings 2145 "Solid state synthesis of CdS quantum dots through laser direct writing"
<p>The dataset includes two files: the word file describe the type of sample and the procedures used to pick up the data; the excel file includes the raw data used to obtain the plots of figure 4.</p> <p>in the following is reported the description of figure 4 as wrote in the paper.</p> <p>PL emission of CdDBX QDs synthetized with single pulses at 355 nm, 10 ps and pulse energy varied between 4.67 to 7.6 μJ. (a), (b) and (c) are respectively the emission in blue, green and red range as defined in methodology. Graph (d) shows the change of the ratio between the red and green emission from the same points.</p>
FIGURE 5 from conference paper AIP Proceedings 2145 "Solid state synthesis of CdS quantum dots through laser direct writing"
<p>The dataset contains two files: the word file describe the type of sample examined and the procedure used to obtain the DOIs used to obtain the plots. The second file is the excel file that includes the raw data used to obtain the plots reported in the figure 5 of the paper.</p> <p>In the following is reported the description of figure 5 as written in the paper:</p> <p>PL emission of CdDBX QDs synthetized with scanlines at 355 nm, 10 ps overlapping pulse of about 99.5% and</p> <p>pulse energy varied between 4.67 to 7.6 μJ. (a), (b) and (c) are respectively the emission in blue, green and red range as defined</p> <p>in methodology. Graph (d) shows the change of the ratio between the red and green emission from the same point. Point “X” is</p> <p>censored and not used to fit the model.</p>
Three-Enzyme Phosphorylase Cascade Immobilized on Solid Support for Biocatalytic Synthesis of Cello-oligosaccharides
<p>We provide here the underlying data of the scientific publication "Three-Enzyme Phosphorylase Cascade Immobilized on Solid Support for Biocatalytic Synthesis of Cello-oligosaccharides". Please find the abstract below:</p> <p>Enzyme cascades are promising for multistep biocatalytic synthesis, but their effective use beyond the proof-of-concept stage is challenging. Strategies to recycle the individual enzymes are critical for the applicability of such cascades. Immobilization on solid support is well developed for single enzymes but remains difficult for enzyme ensembles. Here, we show a controlled co-immobilization of three glycoside phosphorylases to establish a highly active and recyclable biocatalyst for the conversion of sucrose and glucose into soluble (short-chain) cello-oligosaccharides. We use protein fusion with the binding module Z<sub>basic2 </sub>to enable non-covalent surface tethering of all enzymes according to a uniform principle and in a programmable fashion. We thus achieve loading of the phosphorylases in an activity ratio optimal for the overall conversion rate and for controlling the cello-oligosaccharide chain length (≤ 6), hence the solubility, in the reaction. We demonstrate efficient production of ~12 g/L cello-oligosaccharides in 5 reaction cycles with integrated enzyme re-use. This study presents a major advance toward the practical use of systems bio-catalysis on solid support.</p>
Signal feeds for creating the music mixes for comparison of wave field synthesis, surround, and stereo
<p>Wav files for the comparison of wave field synthesis, surround, and stereo listening test, see</p> <p>C. Hold, H. Wierstorf, A. Raake, The Difference Between Stereophony and Wave Field Synthesis in the Context of Popular Music, in 140th AES Convention, 2016.</p>
Recordings for loudness analysis of the music mixes for comparison of wave field synthesis, surround, and stereo
<p>Mat files of live recordings of the music mixes for the comparison of wave field synthesis, surround, and stereo listening test. The recordings were performed at different levels and were analyzed with a loudness model afterwards for adjusting the levels. For details, see</p> <p>C. Hold, H. Wierstorf, A. Raake, The Difference Between Stereophony and Wave Field Synthesis in the Context of Popular Music, in 140th AES Convention, 2016.</p>
Object-based audio scene files for variations of the spatial arrangement in pop mixes for Wave Field Synthesis
<p>This entry contains object-based audio meta-data to generate the mixes published at http://dx.doi.org/10.5281/zenodo.61000.</p> <p>Have a look at README.md for further details.</p>
Listening test results for sound field synthesis localization experiment -- head movement data
<p>This data set contains recorded head movements listeners did during several localisation tasks in the context of sound field synthesis. This is an add-on to the actual localisation results provided by [1].</p> <p>[1] Wierstorf, H. (2016). Listening test results for sound field synthesis localization experiment [Data set]. Zenodo. http://doi.org/10.5281/zenodo.55439</p>
Development of an Amine Transaminase-Lipase Cascade for Chiral Amide Synthesis under Flow Conditions
<p>The use of multienzymatic systems has gained increasing attention as a method of choice for complex (asymmetric) syntheses. Incompatibilities between substrates, reagents and/or enzymes in one-pot batch conditions can hamper the applicability of a pursued cascade, so the use of flow systems provide useful synthetic solutions. The implementation of immobilised enzymes in continuous flow reactors allows the compartmentalisation and segregation of the enzymes in separate reactors, leading to otherwise disfavoured reaction cascades. Here, an amine transaminase and a lipase have been immobilised on polymer-coated controlled porosity glass carrier materials and studied for the first time together in the transamination of a prochiral ketone followed by acylation of the corresponding chiral amine in flow mode, two incompatible transformations under batch. Thus, the preparation of (<em>R</em>)-<em>N</em>-(1-phenoxypropan-2-yl)acetamide was accomplished after optimisation of the reaction conditions.</p>
Integrated Preservation of Water Activity as Key to Intensified Chemoenzymatic Synthesis of Bio-Based Styrene Derivatives
<p>The valorization of lignin-derived feedstocks by catalytic means enables their defunctionalization and upgrading to valuable products. However, the development of productive, safe, and low-waste processes remains challenging. This paper explores the industrial potential of a chemoenzymatic reaction performing the decarboxylation of bio-based phenolic acids in wet cyclopentyl methyl ether (CPME) by immobilized phenolic acid decarboxylase from <em>Bacillus subtilis</em>, followed by a base-catalyzed acylation. Key-to-success is the continuous control of water activity, which fluctuates along the reaction progress, particularly at high substrate loadings (triggered by different hydrophilicities of substrate and product). A combination of experimentation, thermodynamic equilibrium calculations, and MD simulations revealed the change in water activity which guided the integration of water reservoirs and allowed process intensification of the previously limiting enzymatic step. With this, the highly concentrated sequential two-step cascade (400 g·L–1) achieves full conversions and affords products in less than 3 h. The chemical step is versatile, accepting different acyl donors, leading to a range of industrially sound products. Importantly, the finding that water activity changes in intensified processes is an academic insight that might explain other deactivations of enzymes when used in non-conventional media.</p>
Supplementary Information for "Biocatalytic Ether Lipid Synthesis by an Archaeal Glycerolprenylase" - Experimental Data
<p>This is the external Supplementary Information for our publication "Biocatalytic Ether Lipid Synthesis by an Archaeal Glycerolprenylase", freely available as a preprint from <em>ChemRxiv </em>(<a href="https://doi.org/10.26434/chemrxiv-2024-2lmv8-v2">https://doi.org/10.26434/chemrxiv-2024-2lmv8-v2</a>) and as a peer-reviewed publication from <em>Angewandte Chemie</em> (<a href="https://doi.org/10.1002/anie.202412597">https://doi.org/10.1002/anie.202412597</a>).</p> <p>The .zip files contain the raw data and metadata for all items (supplementary and main text) as well as the calculation results underlying each figure panel. This includes UV, fluorescence and NMR data. The zip files below also contain the ChimeraX session used to prepare all figure panels, the results of crystallization screens and reports on all MPLC runs used for the purification of pyrophosphate substrates. The <strong>NMR data for the synthesized compounds</strong> are described in the un-zipped metadata sheet which is separately stored below.</p> <p>The <strong>computational results</strong> contributed by Sangwar Wadtey Oung are stored in a separate zenodo entry (<a href="../doi/10.5281/zenodo.10635216">https://zenodo.org/doi/10.5281/zenodo.10635216</a>).</p> <p>This work was enabled by our previous studies on continuous reaction monitoring of phosphate-releasing transformations (<a href="https://doi.org/10.1021/acs.analchem.1c05356">https://doi.org/10.1021/acs.analchem.1c05356</a>), which itself builds on principles of spectral unmixing (<a href="https://doi.org/10.1002/cbic.202000204">https://doi.org/10.1002/cbic.202000204</a>), isosbestic normalization (<a href="https://doi.org/10.1002/cbic.202200744">https://doi.org/10.1002/cbic.202200744</a>) and thermodynamic reaction control in enzymatic equilibrium systems (<a href="https://doi.org/10.1021/acscatal.1c02589">https://doi.org/10.1021/acscatal.1c02589</a> & <a href="https://doi.org/10.1002/anie.202218492">https://doi.org/10.1002/anie.202218492</a> & <a href="https://doi.org/10.1002/adsc.201901230">https://doi.org/10.1002/adsc.201901230</a>).</p>
Jigsaw Teapot - Synthesis of 3D jigsaw puzzles over freeform 2-manifolds.
<p>This 3D puzzle has been created using tools and algorithms developed at the Technion, and are part of the IRIT geometric modeling kernel (<a href="https://www.cs.technion.ac.il/~irit/">https://www.cs.technion.ac.il/~irit/</a>).</p> <p>This specific Utah teapot puzzle model has been created using function composition of puzzle tiles over the shell geometry of a teapot.</p> <p>Puzzle model is provided, in parts, in OBJ file format.</p>
Jigsaw Duck - Synthesis of 3D jigsaw puzzles over freeform 2-manifolds.
<p>This 3D puzzle has been created using tools and algorithms developed at the Technion, and are part of the IRIT geometric modeling kernel (<a href="https://www.cs.technion.ac.il/~irit/">https://www.cs.technion.ac.il/~irit/</a>).</p> <p>This specific duck puzzle model has been created using function composition of puzzle tiles over the shell geometry of a duck.</p> <p>Puzzle model is provided, in parts, in OBJ file format.</p>
Kinetic modeling of phosphorylase-catalyzed iterative β-1,4-glycosylation for degree of polymerization-controlled synthesis of soluble cello-oligosaccharides
<p>We provide here the underlying data of the publication "Kinetic modeling of phosphorylase-catalyzed iterative β-1,4-glycosylation for degree of polymerization-controlled synthesis of soluble cello-oligosaccharides". Please find the abstract below.</p> <p><strong>Background: </strong>Cellodextrin phosphorylase (CdP; EC 2.4.1.49) catalyzes the iterative β-1,4-glycosylation of cellobiose using α-D-glucose 1-phosphate as the donor substrate. Cello-oligosaccharides (COS) with a degree of polymerization (DP) of up to 6 are soluble while those of larger DP self-assemble into solid cellulose material. The soluble COS have attracted considerable attention for their use as dietary fibers that offer a selective prebiotic function. An efficient synthesis of soluble COS requires good control over the DP of the products formed. A mathematical model of the iterative enzymatic glycosylation would be important to facilitate target-oriented process development.<br> <strong>Results: </strong>A detailed time-course analysis of the formation of COS products from cellobiose (25 mM, 50 mM) and α-D-glucose 1-phosphate (10–100 mM) was performed using the CdP from <em>Clostridium cellulosi</em>. A mechanism-based, Michaelis–Menten type mathematical model was developed to describe the kinetics of the iterative enzymatic glycosylation of cellobiose. The mechanistic model was combined with an empirical description of the DP-dependent self-assembly of the COS into insoluble cellulose. The hybrid model thus obtained was used for kinetic parameter determination from time-course fits performed with constraints derived from initial rate data. The fitted hybrid model provided excellent description of the experimental dynamics of the COS in the DP range 3–6 and also accounted for the insoluble product formation. The hybrid model was suitable to disentangle the complex relationship between the process conditions used (i.e., substrate concentration, donor/acceptor ratio, reaction time) and the reaction output obtained (i.e., yield and composition of soluble COS). Model application to a window-of-operation analysis for the synthesis of soluble COS was demonstrated on the example of a COS mixture enriched in DP 4.<br> <strong>Conclusions:</strong> The hybrid model of CdP-catalyzed iterative glycosylation is an important engineering tool to study and optimize the biocatalytic synthesis of soluble COS. The kinetic modeling approach used here can be of a general interest to be applied to other iteratively catalyzed enzymatic reactions of synthetic importance.</p>
ZnO-Promoted Inverse ZrO2–Cu Catalysts for CO2-Based Methanol Synthesis under Mild Conditions
<p>Datasets supporting the publication 'ZnO-Promoted Inverse ZrO<sub>2</sub>–Cu Catalysts for CO<sub>2</sub>-Based Methanol Synthesis under Mild Conditions': catalyst evaluation data (Excel), XRD (Origin), crystallite sizes, Cu surface area, CO<sub>2</sub> uptake, H<sub>2</sub>-TPR, CO<sub>2</sub>-TPD, CO-DRIFTS profiles (CSV)</p>
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.