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65 results for “Trebouxiophyceae”
Fig. 1 in Species delimitation polyphasic approach reveals Meyerella similis sp. nov.: a new species of "small green balls" within the Chlorella-clade (Trebouxiophyceae, Chlorophyta)
Fig. 1 Morphology of strains ACSSI 346 a, ACSSI 362 b, and ACSSI 363 c cells. The inset shows a more detailed image of an adult cell. The autospores are shown with arrows. THUNDER image of strain ACSSI 346 d. The ciliates with Meyerella cells: Pseudoblepharisma sp. e, Holophrya sp. f. Drawings of light microscopical characters of studied strains (g). 1 – young cell; 2– adult cell; 3,4 – autosporangium. Scale bar a–f: 10 μm, g: 2 μm
FIGURE 4 in Vulcanochloris (Trebouxiales, Trebouxiophyceae), a new genus of lichen photobiont from La Palma, Canary Islands, Spain
FIGURE 4. Vulcanochloris, gen. nov. Morphology in a light or a confocal microscope. A–I. Vulcanochloris canariensis, sp. nov. A– C. Spherical or oval vegetative cells. B–E. Crenulate chloroplast. F−G. Shallowly lobed chloroplast. A−C, H. One distinct, centrally positioned pyrenoid, frequently containing one to several spherical incisions. I. Asexual reproduction by 16–64 aplanospores formed in spherical or ellipsoidal sporangia. J−U. Vulcanochloris symbiotica sp. nov. J, K, N. Spherical, oval and oviform vegetative cells. K−M. Deeply lobed chloroplast. N, O. Shallowly lobed chloroplast. P, Q. Crenulate chloroplast. R. Echinate chloroplast. K, N, P. One distinct pyrenoid located in its centre. N. One to several spherical incisions in pyrenoid. S, T. Asexual reproduction by 32 aplanospores or 128 zoospores produced in spherical or ellipsoidal sporangia. U. Zoospores drop-shaped, naked, with two apical flagella and a simple basal chloroplast. V−DD. Vulcanochloris guanchorum, sp. nov. V, W. Spherical, occasionally oval vegetative cells. V. Chloroplast in young cells in the central position with several lobes spreading towards the cells periphery. W−Y. Deeply lobed chloroplast. Z−AA. Shallowly lobed chloroplast. BB. One distinct, centrally positioned pyrenoid, often containing one to several spherical incisions. CC, DD. Asexual reproduction by 16−32 aplanospores or 64−128 zoospores produced in spherical or ellipsoidal sporangia. Scale bars = 5 μm.
FIGURE 3 in Vulcanochloris (Trebouxiales, Trebouxiophyceae), a new genus of lichen photobiont from La Palma, Canary Islands, Spain
FIGURE 3. Bayesian analysis based on the ITS rDNA dataset. Values at the nodes indicate statistical support estimated by three methods— MrBayes posterior-node probability (left), maximum-likelihood bootstrap (middle), and maximum parsimony bootstrap (right). Asterisk represents full support. Scale bar shows the estimated number of substitutions per site. Newly sequenced strains are marked in bold.
FIGURE 2 in Vulcanochloris (Trebouxiales, Trebouxiophyceae), a new genus of lichen photobiont from La Palma, Canary Islands, Spain
FIGURE 2. Bayesian analysis based on the rbcL dataset. Values at the nodes indicate statistical support estimated by three methods— MrBayes posterior-node probability (left), maximum-likelihood bootstrap (middle), and maximum parsimony bootstrap (right). Asterisk represents full support. Scale bar shows the estimated number of substitutions per site. Newly sequenced strains are marked in bold.
FIGURE 1 in Vulcanochloris (Trebouxiales, Trebouxiophyceae), a new genus of lichen photobiont from La Palma, Canary Islands, Spain
FIGURE 1. Vulcanochloris canariensis, gen. et sp. nov.. Chloroplast morphology and ultrastructure. A, B. A deeply lobed type of chloroplast. C, D. Shallowly lobed type of chloroplast. E, F. Crenulate type of chloroplast. G, H. Echinate type of chloroplast. I. Parietal type of chloroplast. J. High number of small starch grains surrounding the pyrenoid. K–M. Pyrenoid irregularly transversed by inclusions bearing a close structural resemblance to the chloroplast thylakoids. N, O. One to several electron-lucent, spherical to elongated regions frequently formed within the pyrenoid matrix. P. Higher number (more than 8) of the electron-lucent regions formed within the pyrenoid matrix. Scale bars = 5 μm (A–J); 1 μm (K–P).
FIGURE 4 in Polulichloris henanensis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga
FIGURE 4. Phylogenetic position of Polulichloris henanensis within class Trebouxiophyceae (Chlorophyta), based on 18S rDNA + rbcL sequences. The analysis was based on reduced alignment with an outgroup formed by the chlorophycean Chlamydomonas bilatus. The tree was inferred using PAUP*4.0 with the GTR + I + G evolutionary model. Numbers at branches correspond to MrBayes posterior probabilities (BPP)/maximum likelihood (ML) bootstrap values. Values below 0.95 BPP and 50% ML bootstrap support are not shown. Scale bar shows estimated number of substitutions per site.
FIGURE 1 in Polulichloris henanensis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga
FIGURE 1. Morphology of Polulichloris henanensis strain FACHB-1765. A–G: young vegetative cells. H, I: mature vegetative cell. J‒O: autosporangium. P: vegetative cells and liberation of autospores. Scale bars: A‒D = 2 μm, E–P = 5 μm.
FIGURE 2 in Polulichloris henanensis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga
FIGURE 2. Ultrastructure of Polulichloris henanensis. C: chloroplast, P: pyrenoid, SE: starch envelope, S: starch grains. Cell of P. henanensis with a parietal and cup-shaped chloroplast, pyrenoid bisected by a few thylakoid bands, and starch envelope composed of 2–4 plates surrounding the pyrenoid. A. Young cell. B–D. Vegetative cell.
FIGURE 3 in Polulichloris henanensis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta), a novel subaerial coccoid green alga
FIGURE 3. Phylogenetic position of Polulichloris henanensis within class Trebouxiophyceae (Chlorophyta), based on 18S rDNA sequences. The analysis was based on reduced alignment with an outgroup formed by the chlorophycean species Chlamdomonas rosae. The tree was inferred using PAUP*4.0 with the TrNef + I + G evolutionary model. Numbers at branches correspond to MrBayes posterior probabilities (BPP)/maximum likelihood (ML) bootstrap values. Values below 0.95 BPP and 50% ML bootstrap support are not shown. Scale bar shows estimated number of substitutions per site.
FIGURE 2 in Phyllosiphon ari sp. nov. (Watanabea clade, Trebouxiophyceae), a new parasitic species isolated from leaves of Arum italicum (Araceae)
FIGURE 2. Ultrastructure of Phyllosiphon ari sp. nov. A. Cross section of parasitic filaments with thick cell wall containing endospores. B. Detail of an endospore with parietal chloroplast and large extraplastidial oil droplet. C. Autosporangium with autospores inside. Note the autospore released by rupture of autosporangial cell wall, strain CAUP H8803. D. Detail of chlorelloid cell with mitochondria, plastid containing plastoglobuli and starch grains, and extraplastidial oil droplet, strain CAUP H8803. Abbreviations: c, chloroplast; cw, cell wall; m, mitochondria; n, nucleus; o, oil droplet; s, starch grain. Scale bars: 0.5 μm (A–B, D), 2 μm (C).
FIGURE 4 in Phyllosiphon ari sp. nov. (Watanabea clade, Trebouxiophyceae), a new parasitic species isolated from leaves of Arum italicum (Araceae)
FIGURE 4. Bayesian analysis of Trebouxiophyceae based on the rbcL dataset. Numbers at nodes indicate statistical support (BPP> 0.95/ ML> 50 %/MP> 50 %). Thick branches represent nodes receiving the highest BPP support (1.00). The sequences newly acquired in this study are shown in bold. The scale bar shows the estimated number of substitutions per site.
FIGURE 1 in Phyllosiphon ari sp. nov. (Watanabea clade, Trebouxiophyceae), a new parasitic species isolated from leaves of Arum italicum (Araceae)
FIGURE 1. Morphology of Phyllosiphon ari sp. nov. A–B. A leaf of Arum at different stages of infection caused by Phyllosiphon ari. C. Branching filaments in the leaf parenchyma containing endospores. D. Detail of P. ari endospores released from filaments. E. Autosporangium of P. ari, strain N8. F. Vegetative cells with a remnant of the mother cell wall, strain N8. G. Vegetative cell and two-celled autosporangium, strain N8. H. Four-celled autosporangium, strain KRK-12. I. Vegetative cell with a detail of an ornamented cell wall, strain N5. J. Vegetative cells, strain N5. K. Vegetative cells, strain N6. L. Four-celled autosporangium, vegetative cells and autospore, strain N6. Scale bars: 1 cm (A–B), 20 μm (C), 5 μm (D–L).
FIGURE 3 in Phyllosiphon ari sp. nov. (Watanabea clade, Trebouxiophyceae), a new parasitic species isolated from leaves of Arum italicum (Araceae)
FIGURE 3. Bayesian analysis of Trebouxiophyceae based on the 18S rDNA dataset. Numbers at nodes indicate statistical support (BPP> 0.95/ML> 50 %/MP> 50 %). Thick branches represent nodes receiving the highest BPP support (1.00). The sequences newly acquired in this study are shown in bold. The scale bar shows the estimated number of substitutions per site.
FIGURE 1 in Asterochloris sejongensis sp. nov. (Trebouxiophyceae, Chlorophyta) from King George Island, Antarctica
FIGURE 1. Light micrographs and confocal reconstructions of the chloroplast structures in Asterochloris sejongensis sp. nov. (A, B) Parietal lobed chloroplast in young cell. (C, D) Deeply lobed chloroplast; (E, F) crenulated chloroplast; and (G, H) deeply lobed chloroplast with flat lobe ends in the mature cell. (I, J) Aplanospores. Scale bar=5 μm.
FIGURE 4 in Asterochloris sejongensis sp. nov. (Trebouxiophyceae, Chlorophyta) from King George Island, Antarctica
FIGURE 4. Predicted secondary structures of the ITS1 (A) and ITS2 (B) transcripts of Asterochloris sejongensis sp. nov. (2015KGS- 007A) derived by a comparison of the closely related species A. woessiae. Base changes between two Asterochloris genotypes are indicated: the boxed base pair indicates hemi-compensatory base changes (hemi-CBCs); the grey circles indicate single base changes; and the large boxes indicate changes of the ends in the helices. ITS2 transcripts, highly conserved U-U mismatches and UGGU motifs are marked with []. The two star markers in the ITS2 transcript structure indicate specific base changes in the Asterochloris sejongensis sp. nov. (2015KGS-007A) clades.
FIGURE 3 in Asterochloris sejongensis sp. nov. (Trebouxiophyceae, Chlorophyta) from King George Island, Antarctica
FIGURE 3. Bayesian majority role tree based on concatenated ITS rDNA and actin gene sequences. The numbers on each node represent posterior probabilities (left) and bootstrapping values (right). The bold branches indicate strongly supported values (pp=1.00 and ML=100%).
FIGURE 2 in Asterochloris sejongensis sp. nov. (Trebouxiophyceae, Chlorophyta) from King George Island, Antarctica
FIGURE 2. Transmission electron micrographs of 2015KGS-007A. (A) Vegetative cell of 2015KGS-007A. (B) Spore cell of 2015KGS- 007A. (C) Magnified image of thylakoid and chloroplast membrane. (D) Pyrenoglobuli in the chloroplast. Cp, chloroplast; Mt, mitochondria; N, nucleus; Pg, pyrenoglobuli; Th, thylakoid.
FIGURE 4. TCS haplotype network inferred from ITS-2 in The polyphasic approach revealed new species of Chloroidium (Trebouxiophyceae, Chlorophyta)
FIGURE 4. TCS haplotype network inferred from ITS-2 rDNA sequences of Chloroidium saccharophilum. This network was inferred using the algorithm described by Clement et al. (2002). Sequence nodes corresponding to samples collected from different geographical region and from different habitats.
FIGURE 6 in The polyphasic approach revealed new species of Chloroidium (Trebouxiophyceae, Chlorophyta)
FIGURE 6. Morphology and phenotypic plasticity of the new Chloroidium species. A.–G. C. antarcticum; H.–M. C. viscosum; N.–V. C. arboriculum; scale bar = 10 μm.
FIGURE 5. TCS haplotype network inferred from ITS-2 in The polyphasic approach revealed new species of Chloroidium (Trebouxiophyceae, Chlorophyta)
FIGURE 5. TCS haplotype network inferred from ITS-2 rDNA sequences of Chloroidium ellipsoideum and C. lichenum. This network was inferred using the algorithm described by Clement et al. (2002). Sequence nodes corresponding to samples collected from different geographical region and from different habitats.
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