Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
985
datasets available to search
ShareScore release 0.7.1
Dataset results
985 results for “cytotoxicity”
Figure 2 in Cytotoxicity of iodine-131 radiopharmaceutical in tumor and non-tumor human cells and radioprotection by integral juices of Vitis labrusca L.
Figure 2. Mean absorbance and standard deviation of HepG2/C3A cells treated with conventional and organic grape juices, exposed or not to UV-C irradiation (10 and 20 µL/mL), treated alone or in cytoprotective tests with I-131 (1.85 MBq/mL). CO: Control; 1x104 cells per well, incubated for 24 and 48 hours, n = 3, Tukey test. *Statistically significant result compared to Control; #Statistically significant result compared to treatment with I-131; &Statistically significant result compared to treatment with the same juice concentration, without I-131.
Figure 1 in Molecular docking studies and evaluation of the antiretroviral activity and cytotoxicity of the species Lafoensia pacari Saint-Hilaire
Figure 1. Profile of 1H-NMR spectra of subfractions obtained from the ethanolic extract of L. pacari classified by (RT%) activity of each subfraction.
Figure 1 in Cytotoxicity of iodine-131 radiopharmaceutical in tumor and non-tumor human cells and radioprotection by integral juices of Vitis labrusca L.
Figure 1. Mean absorbance and standard deviation of HepG2/ C3A (A) and MRC-5 (B) cells treated with I-131. CO: Control; 1x104 cells per well, incubated for 24 and 48 hours, n = 3. *Statistically significant result compared to Control (p <0.05, Dunnett's test).
Figure 1 in The employment of a conformal polydopamine thin layer reduces the cytotoxicity of silver nanoparticles
Figure 1. Characterization of NPs: (a) UV-Vis absorption spectra of NPs, TEM images of PDOP (b and b ), AgNP (c and c ), and AgNP@PDOP NP (d and d ) systems at different 1 2 1 2 1 2 magnifications. Arrows indicate the thickness of the PDOP layer on the AgNPs.
Figure 3 in The employment of a conformal polydopamine thin layer reduces the cytotoxicity of silver nanoparticles
Figure 3. Representative inverted microscopy images of Caco2 cell lines after 24 h of NP exposures at different concentrations.
Assessing the Cytotoxicity of Phenolic and Terpene Fractions Extracted from Iraqi Prunus arabica on AMJ-13 and SK-GT-4 Human Cancer Cell Lines
<p>Breast and esophagus cancer are the most aggressive and prominent causes of death worldwide. In addition, these cancers showed resistance to current chemotherapy regimens with limited success rates and fatal outcomes. Recently many studies reported the significant cytotoxic effects of phenolic and terpene fractions extracted from various <em>Prunus</em> species against different cancer cell lines. This suggests the probability to be a candidate as an alternative or adjuvant to the current chemotherapeutic regimens. The study aimed to evaluate the cytotoxicity of phenolic and terpene fractions extracted from Iraqi <em>Prunus arabica</em> on breast (AMJ-13) and esophagus (SK-GT-4) cancer cell lines by using the MTT assay. Analysis using Chou-Talalay method performed to assess the synergistic effect between the extracted fractions and chemotherapeutic agent (docetaxel). Moreover, HPLC analysis has been conducted for the quantitative determination of different bioactive molecule of both phenolic and terpene fractions in the extract. According to the findings, the treatment modalities significantly decreased cancer cell viability of AMJ-13 and SK-GT-4 and had insignificant cytotoxicity on the normal cells (normal human fibroblast cell line) (all less than 50% cytotoxicity). Analyzing with Chou-Talalay showed a strong synergism with docetaxel on both cancer cell lines (higher cytotoxicity even in low concentrations) and failed to induce a cytotoxicity on the normal cells. Important flavonoid glycosides and terpenoids were detected by HPLC in the particularly ferulic acid, catechin, chlorogenic acid, B sitosterol, and campesterol. In conclusion, the extracted fractions selectively inhibited the proliferation of both cancer cell and showed minimal cytotoxicity on normal cells. Thus, the study suggested the possible natural source of selected fractions as breast and esophagus cancer drugs</p>
CyTOF and Flow Cytometry dataset assocaited with "Early-to-mid stage idiopathic Parkinson's disease shows enhanced cytotoxicity and differentiation in CD8 T-cells in females"
<p>This dataset contains all the raw mass cytometry (CyTOF) and flow cytometry fcs files associated with Capelle <i>et al</i>. '<i>Early-to-mid stage idiopathic Parkinson's disease shows enhanced cytotoxicity and differentiation in CD8 T-cells in females',</i> <i><strong>Nature Communications</strong>, <strong>2023</strong>,</i> In Press.</p><p>The dataset contains the following information:</p><p>1, The folder " CoPImmunoPD Flow Zenodo V2.zip " contains all the raw fcs files of flow cytometry analysis and the excel table with marker information of five staining panels in the initial discovery analysis using fresh blood samples. The folder also includes the fcs files of analyzing cytotoxicity potential within CD8 T cells and of validation analyses using cryopreserved samples. Single-color/fluorochrome staining files have also been provided for the relevant experiments in the given subfolders for compensation.</p><p>2, The folder "<a href="https://zenodo.org/api/files/75c910aa-3615-4eff-a201-9d2d46e33ea0/CoPImmunoPD_CyTOF_Zenodo.zip">CoPImmunoPD_CyTOF_Zenodo.zip</a>" contains all the raw fcs files generated from the CyTOF measurements in the initial discovery analysis.</p><p><strong>To reproduce our published Figures, please be assure to first read all the accompanied readme/excel information annotation files deposited in the corresponding folders within the zip files, all the Source Data files of different main and supplementary Figure subpanels, Methods and/or any other relevant sections in our manuscript.</strong></p>
CD8+ T cell-derived CD40L mediates non-canonical cytotoxicity in CD40-expressing cancer cells
Open the record for dataset details and reuse information.
Supplementary data for: Comparison of phenotypic and transcriptomic profiles between HFPO-DA and prototypical PPARα, PPARγ, and cytotoxic agents in wild-type and Ppara-null mouse livers
Open the record for dataset details and reuse information.
Supplementary data for: Comparison of transcriptomic profiles between HFPO-DA and prototypical PPARa, PPARg, and cytotoxic agents in wild-type and PPARa knockout mouse hepatocytes
Open the record for dataset details and reuse information.
Supplementary data for: Comparison of transcriptomic profiles between HFPO-DA and prototypical PPARa, PPARg, and cytotoxic agents in mouse, rat, and pooled human hepatocytes
Open the record for dataset details and reuse information.
Data from: Acute cytotoxicity and increased vascular endothelial growth factor after in vitro nitrogen mustard vapor exposure
<p>RATIONALE: Nitrogen mustard (NM) is a highly toxic alkylating agent. It is classified as chemical threat due to its potential use as a warfare agent. When inhaled, mustard exposure can cause both acute and chronic lung injury. The aims of this study are to develop an in vitro culture system for modeling mustard-induced airway injury and to identify growth factors contributing to airway pathology.</p> <p><br> METHODS: Primary human bronchial epithelial cells co-cultured with primary human pulmonary endothelial cells were exposed to NM (25, 50, 100, 250 or 500 µM) or PBS (control) for 1 hour. Lactate dehydrogenase (LDH) and trans-epithelial electrical resistance (TEER) were measured prior to and 24 hours after NM exposure. Endothelial cultures were then stained for live/dead staining. Exposed airway media was also analyzed for growth factors using Luminex multiplex technology. </p> <p><br> RESULTS: A one-hour NM vapor exposure increased supernatant LDH and decreased TEER at 24 hours for all cultures exposed to NM concentrations greater than or equal to 50 µM. Evidence of endothelial cell death occurred at NM concentrations of 250 and 500 µM. Vascular endothelial growth factor (VEGF) signaling, specifically VEGF-A and placental growth factor (PlGF), increased in NM-exposed co-culture media compared to PBS controls. </p> <p><br> CONCLUSIONS: NM vapor exposure causes significant airway epithelial and endothelial injury. Increased VEGF signaling occurred in airway co-cultures exposed to higher NM concentrations with concurrent endothelial cell death. Future studies are required to validate the role of VEGF signaling in both acute and chronic mustard-induced airway pathology. </p>
Raw Data for the article: A Radioactive-Free Method for the Thorough Analysis of the Kinetics of Cell Cytotoxicity
<p>The cytotoxic activity of T cells and Natural Killer cells is usually measured with the chromium release assay (CRA), which involves the use of 51Chromium (<sup>51</sup>Cr), a radioactive substance dangerous to the operator and expensive to handle and dismiss. The accuracy of the measurements depends on how well the target cells incorporate <sup>51</sup>Cr during labelling which, in turn, depends on cellular division. Due to bystander metabolism, the target cells spontaneously release <sup>51</sup>Cr, producing a high background noise. Alternative radioactive-free methods have been developed. Here, we compare a bioluminescence (BLI)-based and a carboxyfluorescein succinimidyl ester (CFSE)-based cytotoxicity assay to the standard radioactive CRA. In the first assay, the target cells stably express the enzyme luciferase, and vitality is measured by photon emission upon the addition of the substrate d-luciferin. In the second one, the target cells are labelled with CFSE, and the signal is detected by Flow Cytometry. We used these two protocols to measure cytotoxicity induced by treatment with NK cells. The cytotoxicity of NK cells was determined by adding increasing doses of human NK cells. The results obtained with the BLI method were consistent with those obtained with the CRA- or CFSE-based assays 4 hours after adding the NK cells. Most importantly, with the BLI assay, the kinetic of NK cells' killing was thoroughly traced with multiple time point measurements, in contrast with the single time point measurement the other two methods allow, which unveiled additional information on NK cell killing pathways.</p>
Raw Data for the article: Complete intra-laboratory validation of a LAL assay for bacterial endotoxin determination in EBV-specific cytotoxic T lymphocytes
<p>Endotoxin content is a critical factor that affects the safety of biological pharmaceutical products. International pharmacopoeias describe several reference methods to determine endotoxin levels in advanced therapy medicinal product (ATMP) preparations. Administration of ATMPs must be done as rapidly as possible to ensure complete viability and potency of the cellular product. To evaluate the endotoxin content in the shortest time possible, we chose to validate an alternative method based on the use of the Charles River Portable Testing System (PTS) and FDA-approved cartridges, compliant with the requirements of the European Pharmacopoeia and providing results in <20 min. Here, we describe a unique and complete validation approach for instrument, personnel, and analytical method for assessment of endotoxins in ATMP matrices. The PTS system provides high sensitivity and fast quantitative results and uses less raw material and accessories compared with compendial methods. It is also less time consuming and less prone to operator variability. Our validation approach is suitable for a validated laboratory with trained personnel capable of conducting the ATMP release tests, and with very low intra-laboratory variability, and meets the criteria required for an alternative approach to endotoxin detection for in-process and product-release testing of ATMPs.</p>
Investigation the cytotoxicity of newly synthesized quinazo-line–sulfonamide derivatives in human leukemia cell lines and hematopoietic activity in zebrafish embryos.
<p>These videos contain the time lapse imaging of the wild type zebrafish embryos showing the circulation, control (mock 0.5% V/V DMSO) and compound 4a treated embryos at 72 hours post fertilization. The compound 4a specifically blocked the formation of blood and no circulation was seen in these embryos. </p>
Payload-delivering engineered γδ T cells display enhanced cytotoxicity, persistence, and efficacy in preclinical models of osteosarcoma
<p>T cell-based cancer immunotherapy has typically relied on membrane-bound cytotoxicity enhancers such as chimeric antigen receptors expressed in autologous αβ T cells. These approaches are limited by tonic signaling of synthetic constructs and costs associated with manufacturing. γδ T cells are an emerging alternative for cellular therapy, possessing innate anti-tumor activity, potent antibody-dependent cellular cytotoxicity, and minimal alloreactivity. We present an immunotherapeutic platform technology built around the innate properties of the Vγ9Vδ2 T cell, harnessing specific characteristics of this cell type and offering an allo-compatible cellular therapy that recruits bystander immunity. We engineered γδ T cells to secrete synthetic tumor-targeting opsonins in the form of an scFv-Fc fusion protein and a mitogenic IL-15Ra–IL-15 fusion protein (stIL15). Using GD2 as a model antigen, we show that GD2-specific opsonin-secreting Vγ9Vδ2 T cells (stIL15-OPS-γδ T cells) have enhanced cytotoxicity and promote bystander activity of other lymphoid and myeloid cells. Secretion of stIL-15 abrogated the need for exogenous cytokine supplementation and further mediated activation of bystander natural killer cells. Compared to unmodified γδ T cells, stIL15-OPS-γδ T cells exhibited superior in vivo control of subcutaneous tumors and persistence in the blood. Moreover, stIL15-OPS-γδ T cells were efficacious against patient-derived osteosarcomas in animal models and in vitro, where efficacy could be boosted with the addition of zoledronic acid. Together the data identify stIL15-OPS-γδ T cells as a candidate allogeneic cell therapy platform combining direct cytolysis with bystander activation to promote tumor control.</p>
Fig. 8 in The cytotoxic activity of Sponges and Tunicates from Turkish Aegean Sea
Fig. 8 — Microscopic images of PC-3 cells treated with extracts for 24 h (Scale bar: 100 µm)
Fig. 7 in The cytotoxic activity of Sponges and Tunicates from Turkish Aegean Sea
Fig. 7 — Cell viability of PC-3 cell line with various extract concentrations treatment for 24 h
Fig. 6 in The cytotoxic activity of Sponges and Tunicates from Turkish Aegean Sea
Fig. 6 — Microscopic images of SH-SY5Y cells treated with extracts for 24 h (Scale bar: 200 µm)
Fig. 4 in The cytotoxic activity of Sponges and Tunicates from Turkish Aegean Sea
Fig. 4 — Microscopic images of AGS cells treated with extracts for 24 h (Scale bar: 200 µm)
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.