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Supplementary material 4 from: Krčmar S, Kučinić M, Pezzi M, Bruvo Mađarić B (2022) DNA barcoding of the horsefly fauna (Diptera, Tabanidae) of Croatia with notes on the morphology and taxonomy of selected species from Chrysopsinae and Tabaninae. ZooKeys 1087: 141-161. https://doi.org/10.3897/zookeys.1087.78707

Figure S2. NJ tree for the tribes Haematopotini and Heptatomini

Figure 3 from: Krčmar S, Kučinić M, Pezzi M, Bruvo Mađarić B (2022) DNA barcoding of the horsefly fauna (Diptera, Tabanidae) of Croatia with notes on the morphology and taxonomy of selected species from Chrysopsinae and Tabaninae. ZooKeys 1087: 141-161. https://doi.org/10.3897/zookeys.1087.78707

Figure 3 ML phylogenetic tree for the tribes Haematopotini and Heptatomini based on COI sequences of specimens sampled in this work and congeneric sequences from BOLD database of public records. The clades corresponding to MOTUs (as determined by species delimitation methods) are collapsed for simplicity; numbers on the nodes denote ML aLRT support (values lower than 0.70 are not shown). MOTUs containing sequences obtained in this study are marked in blue; the results of the species delineation methods for the newly sequenced samples are presented as vertical bars beside the respective MOTU clades (bPTP in red; ABGD in green; ASAP in yellow; classification into BOLDBINs as assigned by BIN-RESL, with newly established BINs marked in bold font).

Supplementary material 3 from: Krčmar S, Kučinić M, Pezzi M, Bruvo Mađarić B (2022) DNA barcoding of the horsefly fauna (Diptera, Tabanidae) of Croatia with notes on the morphology and taxonomy of selected species from Chrysopsinae and Tabaninae. ZooKeys 1087: 141-161. https://doi.org/10.3897/zookeys.1087.78707

Figure S1. NJ tree for the tribe Chrysopsini

Figure 2 from: Krčmar S, Kučinić M, Pezzi M, Bruvo Mađarić B (2022) DNA barcoding of the horsefly fauna (Diptera, Tabanidae) of Croatia with notes on the morphology and taxonomy of selected species from Chrysopsinae and Tabaninae. ZooKeys 1087: 141-161. https://doi.org/10.3897/zookeys.1087.78707

Figure 2 Maximum likelihood (ML) phylogenetic tree for the tribe Chrysopsini based on COI sequences of specimens sampled in this work and congeneric sequences from BOLD database of public records. The clades corresponding to MOTUs (as determined by species delimitation methods) are collapsed for simplicity; numbers on the nodes denote ML aLRT support (values lower than 0.70 are not shown). MOTUs containing sequences obtained in this study are marked in red; the results of the species delineation methods for the newly sequenced samples are presented as vertical bars beside the respective MOTU clades (bPTP in red; ABGD in green; ASAP in yellow; classification into BOLDBINs as assigned by BIN-RESL).

Supplementary material 5 from: Krčmar S, Kučinić M, Pezzi M, Bruvo Mađarić B (2022) DNA barcoding of the horsefly fauna (Diptera, Tabanidae) of Croatia with notes on the morphology and taxonomy of selected species from Chrysopsinae and Tabaninae. ZooKeys 1087: 141-161. https://doi.org/10.3897/zookeys.1087.78707

Figure S3. NJ tree for the tribes Tabanini and Diachlorini

Supplementary material 2 from: Krčmar S, Kučinić M, Pezzi M, Bruvo Mađarić B (2022) DNA barcoding of the horsefly fauna (Diptera, Tabanidae) of Croatia with notes on the morphology and taxonomy of selected species from Chrysopsinae and Tabaninae. ZooKeys 1087: 141-161. https://doi.org/10.3897/zookeys.1087.78707

COI multiple sequence alignments

Figure 4 from: Krčmar S, Kučinić M, Pezzi M, Bruvo Mađarić B (2022) DNA barcoding of the horsefly fauna (Diptera, Tabanidae) of Croatia with notes on the morphology and taxonomy of selected species from Chrysopsinae and Tabaninae. ZooKeys 1087: 141-161. https://doi.org/10.3897/zookeys.1087.78707

Figure 4 ML phylogenetic tree for the tribes Tabanini and Diachlorini based on COI sequences of specimens sampled in this work and congeneric sequences from BOLD database of public records. The clades corresponding to MOTUs (as determined by species delimitation methods) are collapsed for simplicity; numbers on the nodes denote ML aLRT support (values < 0.70 are not shown). MOTUs containing sequences obtained in this study are marked in green; the results of the species delineation methods for the newly sequenced samples are presented as vertical bars beside the respective MOTU clades (bPTP in red; ABGD in green; ASAP in yellow; classification into BOLDBINs as assigned by BIN-RESL, with newly established BINs marked in bold font).

Figure 1 from: Krčmar S, Kučinić M, Pezzi M, Bruvo Mađarić B (2022) DNA barcoding of the horsefly fauna (Diptera, Tabanidae) of Croatia with notes on the morphology and taxonomy of selected species from Chrysopsinae and Tabaninae. ZooKeys 1087: 141-161. https://doi.org/10.3897/zookeys.1087.78707

Figure 1 Sampling sites of horseflies (Diptera: Tabanidae) in Croatia: 1 – Branjina, 2 – Desne, 3 – Djedovica (Papuk Mountain), 4 – Donje Maovice, 5 – Kutjevo, 6 – Normanci, 7 – Njivice (Krk Island), 8 – Peruča, 9 – Petrov vrh (Papuk Mountain), 10 – Seona (Našice), 11 – Tugare, 12 – Velika, 13 – Voćin, 14 – Zmajevac. The details about localities can be found in BOLD project CROTA. Acronyms for the countries: HR: Croatia; SLO: Slovenia; HU: Hungary; RS: Republic of Serbia; BH: Bosnia and Herzegovina: IT: Italy.