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930 results for “induced pluripotent stem cells”
Induced Pluripotent Stem Cell Derived Exosomes Nasal Drops for the Treatment of Refractory Focal Epilepsy
ClinicalTrials.gov study NCT05886205. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Modeling and Pharmacological Targeting of Genetic Cardiomyopathy in Children Via Cardiomyocytes Derived From Induced Pluripotent Stem Cells (DMDstem)
ClinicalTrials.gov study NCT03696628. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Derivation of Induced Pluripotent Stem Cells From an Existing Collection of Human Somatic Cells
ClinicalTrials.gov study NCT00801333. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Generation of Marfan Syndrome and Fontan Cardiovascular Models Using Patient-specific Induced Pluripotent Stem Cells
ClinicalTrials.gov study NCT02815072. IPD Sharing: NO. Countries: 1. Publications: 2.
A targeted metabolomics-based assay using human induced pluripotent stem cell-derived cardiomyocytes identifies structural and functional cardiotoxicity potential
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Data from: Collagen vitrigel promotes hepatocytic differentiation of induced pluripotent stem cells into functional hepatocyte-like cells
Differentiation of stem cells to hepatocytes provides an unlimited supply of human hepatocytes and therefore has been vigorously studied. However, to date, the stem cell-derived hepatocytes were suggested to be of immature features. To obtain matured hepatocytes from stem cells, we tested the effect of culturing iPS cell-derived endoderm cells on collagen vitrigel membrane and compared with our previous reported nanofiber matrix. We cultured hiPS cell-derived endoderm cells on a collagen vitrigel membrane and examined the expression profiles, and tested the activity of metabolic enzymes. Gene expression profile analysis of hepatocytic differentiation markers revealed that upon culture on collagen vitrigel membrane, immature markers of AFP decreased, with a concomitant increase in the expression of mature hepatocyte transcription factors and mature hepatocyte markers such as ALB, ASGR1. Mature markers involved in liver functions, such as transporters, cytochrome P450 enzymes, phase II metabolic enzymes were also upregulated. We observed the upregulation of the liver markers for at least 2 weeks. Gene array profiling analysis revealed that hiPS cell-derived hepatocyte-like cells (hiPS-hep) resemble that of the primary hepatocytes. Functions of the CYP enzyme activities were tested in multi-institution and all revealed high CYP1A, CYP2C19, CYP2D6, CYP3A activity, which could be maintained for at least 2 weeks in culture. Taken together, the present approach identified that collagen vitrigel membrane provides a suitable environment for the generation of hepatocytes from hiPS cells that resemble many characteristics of primary human hepatocytes.
Dataset related to article "Neonicotinoid pesticides affect developing neurons in experi-mental mouse models and in human induced pluripotent stem cell (iPSC)-derived neural cultures and organoids"
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Neonicotinoid pesticides affect developing neurons in experi-mental mouse models and in human induced pluripotent stem cell (iPSC)-derived neural cultures and organoids
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Data from: Comparison of osteoclast differentiation protocols from human induced pluripotent stem cells of different tissue origins
<p class="MsoNormal"><span class="apple-converted-space"><span><strong>Background</strong>: </span></span><span class="apple-converted-space"><span>Ever since their discovery, induced pluripotent stem cells (iPSCs) have been extensively differentiated into a large variety of cell types. However, a limited amount of work has been dedicated to differentiating iPSCs into osteoclasts. </span></span><span>While several differentiation protocols have been published, it remains unclear which protocols or differentiation methods are preferable regarding the differentiation of osteoclasts.</span></p> <p class="MsoNormal"><span><strong>Methods</strong>: </span><span>In this study, we compare the osteoclastogenesis capacity of a peripheral blood mononuclear cell (PBMC)-derived iPSC line to a fibroblast-derived iPSC line in conjunction with either embryoid body-based or monolayer-based differentiation strategies. Both cell lines and differentiation protocols were investigated regarding their ability to generate osteoclasts and their inherent robustness and ease of use. The ability of both cell lines to remain undifferentiated while propagating using a feeder-free system was assessed using alkaline phosphatase staining. This was followed by evaluating mesodermal differentiation and the characterization of hematopoietic progenitor cells using flow cytometry. Finally, osteoclast yield and functionality based on resorptive activity, Cathepsin K, and tartrate-resistant acid phosphatase (TRAP) expression were assessed. Results were validated using qRT-PCR throughout the differentiation stages.</span></p> <p class="MsoNormal"><span><strong>Results</strong>: </span><span class="apple-converted-space"><span>Embryoid-body-based differentiation yielded CD45<sup>+</sup>, CD14<sup>+</sup>, and CD11b<sup>+</sup> subpopulations, which in turn differentiated into osteoclasts which demonstrated TRAP positivity, Cathepsin K expression, and mineral resorptive capabilities. This was regardless of which iPSC line was used. Monolayer-based differentiation yielded lower quantities of hematopoietic cells that were mostly CD34<sup>+</sup> and did not subsequently differentiate into osteoclasts.</span></span></p> <p class="MsoNormal"><span class="apple-converted-space"><span><strong>Conclusions</strong>: </span></span><span>The outcome of this study demonstrates the successful differentiation of osteoclasts from iPSCs in conjunction with the embryoid-based differentiation method, while the monolayer-based method did not yield osteoclasts. No differences were observed regarding osteoclast differentiation between the PBMC and fibroblast-derived iPSC lines.</span></p>
Modeling Bronchial Epithelium in Severe Asthma With Human Induced Pluripotent Stem Cells (iPSC)
ClinicalTrials.gov study NCT05616338. IPD Sharing: YES. Countries: 1. Publications: 0.
Blood Collection From Healthy Volunteers and Patients for the Production of Clinical Grade Induced Pluripotent Stem Cell (iPSC) Products
ClinicalTrials.gov study NCT02056613. IPD Sharing: Not stated. Countries: 0. Publications: 3.
Data from: Collagen vitrigel promotes hepatocytic differentiation of induced pluripotent stem cells into functional hepatocyte-like cells
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Data from: Comparison of osteoclast differentiation protocols from human induced pluripotent stem cells of different tissue origins
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Effects of Spaceflight on Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Structure and Function
With extended stays aboard the International Space Station (ISS) becoming commonplace there is a need to better understand the effects of microgravity on cardiac function. We utilized human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to study the effects of microgravity on cell-level cardiac function and gene expression. The hiPSC-CMs were cultured aboard the ISS for 5.5 weeks and their gene expression structure and functions were compared to ground control hiPSC-CMs. Exposure to microgravity on the ISS caused alterations in hiPSC-CM calcium handling. RNA-sequencing analysis demonstrated 2,635 genes were differentially expressed among flight post-flight and ground control samples including genes involved in mitochondrial metabolism. This study represents the first use of hiPSCs to model the effects of spaceflight on human cardiomyocyte structure and function.
Reprogramming-associated aberrant DNA methylation determines hematopoietic differentiation capacity of human induced pluripotent stem cells [PSCderived_HPCs_methylation]
GEO Series GSE60811. Homo sapiens. 10 samples. Type: Methylation profiling by genome tiling array.
Bulk RNA-seq of neuromuscular system models generated from human induced pluripotent stem cells (hiPSCs)
GEO Series GSE226477. Homo sapiens. 15 samples. Type: Expression profiling by high throughput sequencing.
Reprogramming-associated aberrant DNA methylation determines hematopoietic differentiation capacity of human induced pluripotent stem cells
GEO Series GSE60924. Homo sapiens. 181 samples. Type: Expression profiling by array; Methylation profiling by genome tiling array.
Modelling schizophrenia using human induced pluripotent stem cells
GEO Series GSE30737. Homo sapiens. 4 samples. Type: Genome variation profiling by genome tiling array.
CRISPR/Cas9-mediated suppression of A4GALT rescue endothelial cell dysfunction in a human induced pluripotent stem cell derived fabry disease vasculopathy model
GEO Series GSE241251. Homo sapiens. 9 samples. Type: Expression profiling by high throughput sequencing.
Reduced representation bisulfite sequencing (RRBS) profiles of human induced pluripotent stem cells exposed to varied chemicals
GEO Series GSE228658. Homo sapiens. 5 samples. Type: Methylation profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.