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277 results for “novel proteins”
Effect of the growth medium composition on nitrate accumulation in the novel protein crop Lemna minor
<p>Duckweed is a potential alternative protein source for food and feed. However, little is known about the nitrate accumulation in this plant. A high nitrate level in vegetables can indirectly lead to an elevated intake of nitrites and N-nitroso compounds, increasing the risk of diseases for humans and animals. This research hypothesizes that the nitrate accumulation of <em>Lemna minor</em> differs between growing media. Additionally, it evaluates whether legal safety levels of nitrate for human and animal intake are exceeded. The duckweed was grown on (i) rainwater, and (ii) three synthetic media containing different nutrient levels. Furthermore, (iii) biological effluent of swine manure treatment and (iv) aquaculture effluent from pikeperch production were used, as these are potential media for closing nutrient loops in the agriculture sector. It was found that nitrate levels increased with the increasing availability of macronutrients in the water, and pH showed a particularly strong negative correlation with the nitrate levels in the plant. Nevertheless, nitrate content never exceeded 530 mg NO<sub>3</sub> kg<sup>−1</sup> fresh weight. To conclude, <em>Lemna minor</em>'s nitrate content was below safety limits for human consumption in all tested growing media; however, a potential risk for ruminants was observed as these are more sensitive to nitrate conversions in their gastro-intestinal track.</p>
A novel methodological approach to simultaneously extract high quality total RNA and proteins from cortical and trabecular bone
<p>Molecular differences between cortical and trabecular bone, of relevance to understand the pathophysiological bases of bone diseases, can be determined only throughout effective isolation methods for RNA and proteins. Here we present a TRIzol-based method, combining bone pulverization and homogenization, to extract simultaneously total RNA and proteins from human cortical and trabecular bone from the same carrot. RNA integrity and purity were determined as the 260-to-280 nm and 260-to-230 nm absorbance- ratios, and 28S-to-18S rRNA ratio. Protein integrity and quality were evaluated by Comassie Blue staining. RT-qPCR and immunoblotting for bone-specific genes and proteins were performed to verify the suitability of the isolated material in downstream applications. 260-to-280 nm and 260-to-230 nm absorbance ratios were, on average, ≥1.8. Bands on agarose gel were consistent to an intact RNA, with mean 28S-to-18S ratios of1.68±0.35 and 1.88±0.10 for cortical and trabecular bone, respectively. Band patterns after Comassie Blue staining confirmed protein integrity. Successful gene and protein expression analysis, with relevant differences between the two compartments, highlighted the suitability of the material in downstream applications. The method here presented is appropriate and effective for the study of human bone.</p>
Results for Genome scale modeling of the protein secretory pathway reveals novel targets for improved recombinant protein production in yeast
<p>This dataset contains the original results for the paper "Genome scale modeling of the protein secretory pathway reveals novel targets for improved recombinant protein production in yeast". To reproduce all figures in this paper, this dataset is required. The scripts can be found in Github (https://github.com/SysBioChalmers/pcSecYeast)</p>
Supplementary Materials: High-Quality Genome of a novel species belonging to the family Thermosynechococcaceae from Namibia and characterization of its protein expression patterns at elevated temperatures
<p><strong>Supplementary Materials</strong> to the scientific research article "<strong>High-Quality Genome of a novel species belonging to the family<em> Thermosynechococcaceae </em>from Namibia and characterization of its protein expression patterns at elevated temperatures</strong>" in MicrobiologyOpen.</p> <p>Including raw data of: the BCG analysis with antismash, dbCAN3 analysis of carbohydrate active enzymes, eggNOG mapper and KEGG (blastKOALA) functional annotations, whole genome alignments with progressiveMauve, ortholog-based phylogenetics with OrthoFinder, RAST annotations of the novel <em>Thermosynechococcus</em> sp. Okahandja<em> </em>and the type strains <em>Thermosynechococcus lividus</em> PCC 6715-6717, the full length 16S rRNA sequence extracted from the genome, proteomics analyses, the novel <em>T. </em>sp. Okahandja<em> </em>annotated genome, additional STEM microscopy figures and CRISPR analyses.</p>
Identifying in vitro toxicity testing approaches for (novel) proteins in the context of food and feed risk assessment
<p>Annexes to EFSA external scientific report Identifying <em>in vitro</em> toxicity testing approaches for (novel) proteins in the context of food and feed risk assessment</p>
Figure 6 in A novel epidermal gland type in lizards (α-gland): structural organization, histochemistry, protein profile and phylogenetic origins
Figure 6. Transmission electron microscopy of the epithelium from the femoral area of Tropidurus catalanensis. A, general view of the glandular epithelium showing major skin layers and abundance of vesicles inside glandular cells. B–G, magnified view of glandular tissue showing: B, clusters of melanin granules and numerous vesicles; C, aggregations of four different types of vesicles (V1, V2, V3 and V4); D, E, Golgi apparatuses amid secretory vesicles; F, autophagocytic events (arrowheads indicate membrane projections); G, iridophores present in the apical portion of the dermis. Legend: Go, Golgi complex; Ir, iridophore; Me, melanin granule; Nu, nucleus; V1, vesicles type 1; V2, vesicles type 2; V3, vesicles type 3; V4, vesicles type 4.
Figure 3 in A novel epidermal gland type in lizards (α-gland): structural organization, histochemistry, protein profile and phylogenetic origins
Figure 3. Maderson & Chiu's (1970) model of epidermal gland evolution. Steps of the model are briefly described in items A–G.
Figure 2 in A novel epidermal gland type in lizards (α-gland): structural organization, histochemistry, protein profile and phylogenetic origins
Figure 2. Histological structure of unspecialized skin and epidermal glands. Diagrammatic representation of the unspecialized squamate skin and major epidermal gland types, illustrating their respective secretion mechanisms as hypothesized by Maderson (1972).
Figure 5. A in A novel epidermal gland type in lizards (α-gland): structural organization, histochemistry, protein profile and phylogenetic origins
Figure 5. A, unspecialized scales from the pre-cloacal flap of a male Stenocercus caducus (MZUSP-R 82815). B, unspecialized scales from the femoral area of a female Tropidurus chromatops (MZUSP-R 106266). C, scales with α-glands from the femoral area of male T. chromatops (MZUSP-R 106263). Note that β-keratin layers are not present in A and C because they were lost during sample preparation. D, unspecialized scale (stage I) from the humeral region of a female T. chromatops (MZUSP-R 106266). E, detail of a scale with α-gland (stage IV) from the femoral area of a male T. xanthochilus (MZUSP-R 106342). F, detail of a scale with α-gland (stage V) from the pre-cloacal flap of a male T. xanthochilus (MZUSP-R 106342). G, detail of a scale with α-gland (stage VI) from the femoral areal of a male T. xanthochilus (MZUSP-R 106336). H, part of the inner generation of an α-gland from the femoral area of a male Plica plica (MTR 18918) showing the glandular stratum with a large number of secretory vesicles (indicated with an asterisk). I, part of the inner generation of the α-gland from the femoral area of a male T. catalanensis (MZUSP-R 106470) with melanophores transferring melanin granules into glandular cells and numerous melanin granules accumulated in their cytoplasm. J, glandular tissue of the outer generation
Figure 9 in A novel epidermal gland type in lizards (α-gland): structural organization, histochemistry, protein profile and phylogenetic origins
Figure 9. Ancestral state reconstructions of α-gland/flash mark related characters of tropidurid lizards.
Figure 4 in A novel epidermal gland type in lizards (α-gland): structural organization, histochemistry, protein profile and phylogenetic origins
Figure 4. Ventral view of (A) a male Tropidurus chromatops Harvey & Gutberlet, 1998 (MHNC-R 3018) from ~30 km W Florida, Santa Cruz, Bolivia, (B) a male T. melanopleurus Boulenger, 1902 (IBIGEO-R 5331) from Aguas Blancas, Salta, Argentina, (C) a female T. xanthochilus Harvey & Gutberlet, 1998 (MZUSP-R 106321) from Santo Antônio do Leverger, Mato Grosso, Brazil, and (D) a male T. etheridgei Cei, 1982 (AMNH-R 176273) from Filadelfia, Boquerón, Paraguay, illustrating the location and coloration of flash marks observed (or not) on the ventral body of tropidurines. Black squares in (A) indicate body areas from which we collected skin samples for histological examination. In (D) the yellow coloration covering the background of the black flash-marks of T. etheridgei might either represent a transient ontogenetic state or an instance in which a yellow background persists throughout life.
Clinical Safety of a Novel Milk Protein Peptide
ClinicalTrials.gov study NCT01412658. IPD Sharing: Not stated. Countries: 1. Publications: 1.
The Implementation of Novel 'Heavy' Water Techniques for Determining Modulation of Muscle Protein, DNA and Lipid Synthesis Due to Ageing
ClinicalTrials.gov study NCT02152839. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Effect of Novel Antidiabetic Drug Combined With Angiotensin Receptor/Neprilysin Inhibitor on Urinary Protein
ClinicalTrials.gov study NCT05922852. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Omicron BA.4/5-Delta Strain Recombinant Novel Coronavirus Protein Vaccine (CHO Cells)
ClinicalTrials.gov study NCT05850507. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Arginine Feeding: a Novel Strategy to Improve Protein Metabolism in Cancer and the Response to Surgery
ClinicalTrials.gov study NCT00497380. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Data from: Molecular docking and dynamics studies to identify novel active compounds targeting potential breast cancer receptor proteins from an indigenous herb Euphorbia thymifolia Linn
Open the record for dataset details and reuse information.
Evolutionary rate covariation reveals novel protein networks in Mycobacterium tuberculosis
Open the record for dataset details and reuse information.
Data and analysis accompanying "A pan-plant protein complex map reveals deep conservation and novel assemblies"
<p>This repository contains files, instructions to replicate, and internal scripts for the manuscript "A pan-plant protein complex map reveals deep conservation and novel assemblies"</p> <p>See also <a href="http://plants.proteincomplexes.org/">http://plants.proteincomplexes.org</a></p>
Data from: The basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold
Streptococcus pneumoniae is a major human pathogen, and a leading cause of disease and death worldwide. Pneumococcal invasive disease is triggered by initial asymptomatic colonization of the human upper respiratory tract. The pneumococcal serine-rich repeat protein (PsrP) is a lung-specific virulence factor whose functional binding region (BR) binds to keratin-10 (KRT10) and promotes pneumococcal biofilm formation through self-oligomerization. We present the crystal structure of the KRT10-binding domain of PsrP (BR187–385) determined to 2.0 Å resolution. BR187–385 adopts a novel variant of the DEv-IgG fold, typical for microbial surface components recognizing adhesive matrix molecules adhesins, despite very low sequence identity. An extended β-sheet on one side of the compressed, two-sided barrel presents a basic groove that possibly binds to the acidic helical rod domain of KRT10. Our study also demonstrates the importance of the other side of the barrel, formed by extensive well-ordered loops and stabilized by short β-strands, for interaction with KRT10.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.