Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
301
datasets available to search
ShareScore release 0.9.0
Dataset results
301 results for “primary culture”
Primary hepatocyte culture from Oreochromis niloticus fish as a tool for environmental toxicology
<p>Accumulated chemicals have been impairing water quality over decades of human activities. Organisms such as fish are often used for risk assessment, as chemical analysis is insufficient to assess the impact on health. Consequently, researchers have faced challenges in ecotoxicology, concerning the use of animals. Primary and cell line cultures are good alternatives for reducing animal use. This study aims to evaluate the use of primary hepatocytes culture from Oreochromis niloticus as an in vitro tool for screening environmental contaminants and pollutants. Metal cadmium (Cd) is the pollutant model.</p>
Phenotypic screening using waveform analysis of synchronized calcium oscillations in primary cortical cultures
<p><span>At present, <em>in</em> <em>vitro</em> phenotypic screening methods are widely used for drug discovery. In the field of epilepsy research, measurements of neuronal activities have been utilized for predicting efficacy of anti-epileptic drugs (AEDs). Fluorescence measurements of calcium oscillations in neurons are commonly used for measurement of neuronal activities, and some anti-epileptic drugs have been evaluated using this assay technique. However, changes in waveforms were not quantified in previous reports. Here, we have developed a high-throughput screening system containing a new analysis method for quantifying waveforms, and our method has successfully enabled simultaneous measurement of calcium oscillations in a 96-well plate. Features of waveforms were extracted automatically and allowed the characterization of some anti-epileptic drugs using principal component analysis. Moreover, we have shown that trajectories in accordance with the concentrations of compounds in principal component analysis plots were unique to the mechanism of anti-epileptic drugs. We believe that an approach that focuses on the features of calcium oscillations will lead to better understanding of the characteristics of existing anti-epileptic drugs and allow prediction of the mechanism of action (MoA) of novel drug candidates.</span></p>
Evaluation of Autologous Natural Killer Cell Activity in Primary Cancer Cell Cultures
ClinicalTrials.gov study NCT05869994. IPD Sharing: NO. Countries: 1. Publications: 1.
Effectiveness, Implementation and Spillover Effects of a Culturally Adapted DPP in Spanish Primary Care Centers
ClinicalTrials.gov study NCT06871059. IPD Sharing: NO. Countries: 1. Publications: 1.
Culturally Sensitive Depression Care Management for Latino Primary Care Patients
ClinicalTrials.gov study NCT00571454. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Pilot Study of Brief Culturally Adapted Behavioral Activation for Adults With Depression in Primary Care Setting
ClinicalTrials.gov study NCT07120893. IPD Sharing: YES. Countries: 1. Publications: 2.
An Investigation of Tumor Necrosis Factor (TNF)-Alpha in Asthma Using Biopsy Explants and Primary Bronchial Epithelial Cell Cultures
ClinicalTrials.gov study NCT01161303. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Primary Culture of Residual Specimens Obtained From Aspiration of Hepatic Tumor to Predict the Prognosis of the Patients
ClinicalTrials.gov study NCT02587793. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Phenotypic screening using waveform analysis of synchronized calcium oscillations in primary cortical cultures
Open the record for dataset details and reuse information.
Original pictures of isolation, culture and immunofluorescence staining of primary fibroblasts from cervical tissue in vitro
Open the record for dataset details and reuse information.
Data from: Exogenous α-synuclein hinders synaptic communication in cultured cortical primary rat neurons
Amyloid aggregates of the protein α-synuclein (αS) called Lewy Bodies (LB) and Lewy Neurites (LN) are the pathological hallmark of Parkinson's disease (PD) and other synucleinopathies. We have previously shown that high extracellular αS concentrations can be toxic to cells and that neurons take up αS. Here we aimed to get more insight into the toxicity mechanism associated with high extracellular αS concentrations (50-100 μM). High extracellular αS concentrations resulted in a reduction of the firing rate of the neuronal network by disrupting synaptic transmission, while the neuronal ability to fire action potentials was still intact. Furthermore, many cells developed αS deposits larger than 500 nm within five days, but otherwise appeared healthy. Synaptic dysfunction clearly occurred before the establishment of large intracellular deposits and neuronal death, suggesting that an excessive extracellular αS concentration caused synaptic failure and which later possibly contributed to neuronal death.
Hydrogen Peroxide to the Wound Following Surgical Incision Affecting Cultures in Primary Shoulder Arthroplast
ClinicalTrials.gov study NCT04609306. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Brief Culturally Adapted Behavioral Activation Therapy for Adults With Depression in Rural Indonesian Primary Care: A Type-2 Hybrid Effectiveness-Implementation Trial
ClinicalTrials.gov study NCT07373080. IPD Sharing: YES. Countries: 1. Publications: 0.
Data from: Exogenous α-synuclein hinders synaptic communication in cultured cortical primary rat neurons
Open the record for dataset details and reuse information.
Mature primary human osteocytes in mini organotypic cultures secrete FGF23 and PTH1-34-regulated sclerostin
GEO Series GSE227994. Homo sapiens. 20 samples. Type: Expression profiling by high throughput sequencing.
Effect of estrogen and testesterone on muscle primary cultures
GEO Series GSE231324. Homo sapiens. 30 samples. Type: Expression profiling by high throughput sequencing.
Fatostatin drug treatment of mouse primary microglia cultures
GEO Series GSE146864. Mus musculus. 11 samples. Type: Expression profiling by high throughput sequencing.
Bulk transcriptome analysis of Myt1l mutant mouse neuron primary cultures.
GEO Series GSE217554. Mus musculus. 12 samples. Type: Expression profiling by high throughput sequencing.
Primary cultures of glomerular parietal epithelial cells or podocytes with proven origin
GEO Series GSE33714. Mus musculus. 8 samples. Type: Expression profiling by array.
Expression data from Influenza A infected mouse primary tracheal epithelial cell cultures (MTEC), from wild-type, IFNAR1-/-, IL28Ra-/- and IFNAR1-/- IL28Ra-/- double ko
GEO Series GSE43708. Mus musculus. 40 samples. Type: Expression profiling by array.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.