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395 results for “rat model”
Effects of ACTH4-10Pro8-Gly9-Pro10 on anti-inflammatory cytokine (IL-4, IL-10, IL-13) expression in acute spinal cord injury model (Sprague Dawley rats)
<p class="MsoNormal"><span><strong>Background:</strong> Spinal cord injury (SCI) is a destructive neurological and pathological state that causes major motor, sensory and autonomic dysfunctions. It's final neurological outcome determined from both primary and secondary injury process. Neuroinflammation is a key component of the secondary injury mechanisms with local and systemic consequences. </span>A neuroprotective compound, ACTH<sub>4-10</sub>Pro<sup>8</sup>-Gly<sup>9</sup>-Pro<sup>10 </sup>also known as Semax has <span>shown neuroprotective and anti-inflammatory properties. </span>ACTH<sub>4-10</sub>Pro<sup>8</sup>-Gly<sup>9</sup>-Pro<sup>10</sup> <span>also has actively used in the treatment of brain ischemia without serious complication reported. Here we analyzed the effects of </span>ACTH<sub>4-10</sub>Pro<sup>8</sup>-Gly<sup>9</sup>-Pro<sup>10</sup> in regulating inflammatory cascade in SCI by looking at the expression of anti-inflammatory cytokine IL-4, IL-10, IL-13 in acute compression SCI.</p> <p><span><strong>Method: </strong>We do laminectomy in Sprague Dawley rats at the second thoracic vertebrae. After laminectomy we expose the myelum and create mild SCI model with 20gr and severe SCI with 35gr aneurysm clips. </span>ACTH<sub>4-10</sub>Pro<sup>8</sup>-Gly<sup>9</sup>-Pro<sup>10 </sup><span>was administered intranasally to the treatment group and 0,9% NaCl to the control group (placebo). Both group was remain alive and terminated at 3 and 6 hour. </span>The preparations tissue sample were fixed in formalin and examined for immunohistochemistry<span>. Quantitative measurement of anti-inflammatory cytokine (</span>IL-4, IL-10, IL-13) was done in posterior horn with associated anti-monoclonal antibodies.</p> <p> </p> <p><strong>Result:</strong> Rats with mild SCI that were given ACTH<sub>4-10</sub>Pro<sup>8</sup>-Gly<sup>9</sup>-Pro<sup>10</sup> shown greater expression of IL-4, IL-10 and IL-13 at three hour post compression but only IL-10 and IL-13 elevated significantly at six hour. Rats with severe compression in ACTH<sub>4-10</sub>Pro<sup>8</sup>-Gly<sup>9</sup>-Pro<sup>10</sup> group shown greater expression of IL-10, IL-13 at three hour and IL-4, IL-10 at six hour compared with the placebo group.</p> <p> </p> <p><strong>Conclusion: </strong>Administration of ACTH<sub>4-10</sub>Pro<sup>8</sup>-Gly<sup>9</sup>-Pro<sup>10</sup> intranasal can increase anti inflammatory cytokine expression at Sprague Dawley rat model with mild and severe SCI. Expression of anti inflammatory cytokine was greater in mild compression and early hour (3 hour). Further research needs to be done to determine optimal dose and the actual clinical outcome in vivo.</p>
The Role of Proteomics Analysis in Carcinogenesis in a Rat Mammary Cancer Model Induced by DMBA (7,12-dimethylbenz(α)anthracene)
<p>The dataset consists of raw data on protein concentration from a Nanodrop Spectrophotometer and raw data on protein molecular weight from SDS-PAGE</p>
A Model of Rat Non-barrel Somatosensory Cortex Anatomy
<p><strong>A full description of the model is available in the two companion manuscripts: </strong></p> <p><a href="https://www.biorxiv.org/content/10.1101/2022.08.11.503144v3.abstract">Modeling and Simulation of Neocortical Micro- and Mesocircuitry. Part I: Anatomy</a></p> <p><a href="https://www.biorxiv.org/content/10.1101/2023.05.17.541168v5">Modeling and Simulation of Neocortical Micro- and Mesocircuitry. Part II: Physiology and Experimentation</a></p> <p><em>We kindly ask that you cite these papers, as well as the Zenodo repository, in any articles or presentations using the model or any of its constituent components.</em></p> <p>---</p> <p>We present a data-driven computational model of the anatomy of non-barrel primary somatosensory cortex of juvenile rat. The modeling process is based on a previously established workflow for a single cortical column, but is extended here to build a much larger circuit in an atlas-based geometry. Neurons in the model belong to 60 different morphological types and are connected by synapses placed by two established algorithms, one modeling local connectivity determined by axo-dendritic overlap, and one for long-range connectivity between sub-regions. Long-range connectivity is defined with topographic mapping and laminar connectivity profiles, providing intrinsic feed-forward and feedback pathways. Additionally, we incorporate core- and matrix-type thalamocortical projection systems, associated with VPM and POm thalamic nuclei respectively, that enable extrinsic input.</p> <p>The model comprises 211712 neurons in the front limb and jaw subregions and the dysgranular zone of the Paxinos & Watson rat brain atlas, scaled down to juvenile size. It is available in the open <a href="https://github.com/AllenInstitute/sonata">SONATA</a> standard and contains neuron locations and their properties (such as morphological types, cortical layer, etc.), their detailed morphologies, and synaptic connectivity associated with all systems described above. Modeled synapses are associated with their exact location in the dendritic tree, and additional anatomical parameters, such as spine length (where biologically plausible). Extrinsic synaptic connections from neurons in the remainder of non-barrel somatosensory cortex and thalamic inputs are also contained.</p> <p>Note that this is an <em>anatomical</em> model: Parameters and files related to neuronal and synaptic <em>physiology</em> can be found in our <a href="../record/7930276">release of the <em>physiological </em>model</a>.</p> <p><strong>[UPDATE 23/07/17]: </strong>Added a zip archive containing the voxel atlas data used. This comprises the region atlas (brain_regions, hierarchy) and generated voxelized densities for each neuron type ([cell_density]*). All atlas files are in the .nrrd format, best loaded using the python package <a href="https://github.com/BlueBrain/voxcell">voxcell</a>. The atlas files cover the entire S1 regions, with the location of the part of the model released here indicated by <em>published_volume.nrrd. </em><strong>All other files remained unchanged!</strong></p> <p>Please refer to the documentation of the SONATA format for information how to load and analyze the model. A jupyter notebook has been included with basic examples of how to load the data using our open-source packages <a href="https://neurom.readthedocs.io/en/stable/">NeuroM</a> and <a href="https://bluebrainsnap.readthedocs.io/en/stable/">Blue Brain SNAP</a>.</p> <p><em>This study was supported by funding to the Blue Brain Project, a research center of the Ecole polytechnique federale de Lausanne (EPFL), from the Swiss government’s ETH Board of the Swiss Federal Institutes of Technology. RL, JPS and JL were supported by EPSRC under grant number EP/P025072/1. RL was supported by a collaboration grant from EPFL.</em></p>
Dataset underpinning the development of a PBK model for inhalation of TiO2 NPs in rats
<table> <tbody> <tr> <td> <p>This dataset contains concentration-time profiles (expressed as % of the initial dose) of 20 nm TiO2 nanoparticles following a 2-hour inhalation experiment in rats, with tissue and excreta samples taken over a period of 28 days post exposure. The data were extracted from the publication of Kreyling et al. (2019) (<a href="https://doi.org/10.1186/s12989-019-0303-7">https://doi.org/10.1186/s12989-019-0303-7</a>) </p> <p> </p> <p> </p> </td> </tr> </tbody> </table>
Protective effects of <em>Melissa officinalis</em> ethanolic extract on doxorubicin-induced cardiotoxicity in a rat model
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Effects of ACTH4-10Pro8-Gly9-Pro10 on anti-inflammatory cytokine (IL-4, IL-10, IL-13) expression in acute spinal cord injury model (Sprague Dawley rats)
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Early ultrasonic vocalization deficits and related thyroarytenoid muscle pathology in the transgenic TgF344-AD rat model of Alzheimer’s disease
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Blood glucose modulation and safety of efferent vagus nerve stimulation in a type 2 diabetic rat model
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Gut alterations in a chronic kidney disease rat model with diet-induced vascular calcification
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Data from: Quantifying liver-toxic responses from dose-dependent chemical exposures using a rat genome-scale metabolic model
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Serum levels of tau protein increase according to the severity of the injury in DAI rat model
<p><span><span><span><span><span><span><span><span><span><span><span>Traumatic brain injury (TBI) in the form of diffuse axonal injury (DAI) is difficult to diagnose in the early phase of the injury. Early diagnosis of DAI may provide opportunity for developing treatment and management strategies. Tau protein has been demonstrated to increase in the early phase of TBI with high diagnostic accuracy in patients with DAI. We tested the biological plausibility of tau protein using a rat DAI model by evaluating the association between serum tau levels and the severity of brain injury. DAI was induced in animals using the Marmarou model. After a survival of 60 minutes, rats were anesthetized and sacrificed after obtaining blood samples (5ml) from the heart. Eighteen rats were employed in the present study and were randomly subjected to sham-operated control (n=4), mild DAI (n=7), and severe DAI (n=7). Of seven severe DAI rats, two rats that had focal injury caused by skull fracture were excluded in the measurement of tau protein level. The serum levels of tau protein in the rat DAI model were found to increase significantly and consistently according to the severity of the injury. Rats with DAI showed significantly higher serum levels of tau protein compared to sham rats; the severe DAI rats had higher levels of tau than moderate DAI and sham rats (sham vs. mild, <i>P</i>=0.02; mild vs. severe, <i>P</i>=0.02). In conclusion, serum tau protein levels may be useful as a biomarker for diagnosing and estimating the severity of DAI in the early phase.</span></span></span></span></span></span></span></span></span></span></span></p> <div> </div>
Data from: A priori and a posteriori approaches for finding genes of evolutionary interest in non-model species: osmoregulatory genes in the kidney transcriptome of the desert rodent Dipodomys spectabilis (banner-tailed kangaroo rat)
One common goal in evolutionary biology is the identification of genes underlying adaptive traits of evolutionary interest. Recently next-generation sequencing techniques have greatly facilitated such evolutionary studies in species otherwise depauperate of genomic resources. Kangaroo rats (Dipodomys sp.) serve as exemplars of adaptation in that they inhabit extremely arid environments, yet require no drinking water because of ultra-efficient kidney function and osmoregulation. As a basis for identifying water conservation genes in kangaroo rats, we conducted a priori bioinformatics searches in model rodents (Mus musculus and Rattus norvegicus) to identify candidate genes with known or suspected osmoregulatory function. We then obtained 446,758 reads via 454 pyrosequencing to characterize genes expressed in the kidney of banner-tailed kangaroo rats (Dipodomys spectabilis). We also determined candidates a posteriori by identifying genes that were overexpressed in the kidney. The kangaroo rat sequences revealed nine different a priori candidate genes predicted from our Mus and Rattus searches, as well as 32 a posteriori candidate genes that were overexpressed in kidney. Mutations in two of these genes, Slc12a1 and Slc12a3, cause human renal diseases that result in the inability to concentrate urine. These genes are likely key determinants of physiological water conservation in desert rodents.
Therapeutic Potential of Nigella sativa Extract against Inflammatory Markers Interleukin-1ß (IL-1ß) and Tumor Necrosis Factor-α (TNF-α) in Rat Models with High Fat Diet
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Data associated to the study entitled:"Alterations of the nigrostriatal pathway in a 6-OHDA rat model of Parkinson's disease evaluated with multimodal MRI"
<p>Parkinson’s disease is characterized by neurodegeneration of the dopaminergic neurons in the substantia nigra pars compacta. The 6-hydroxydopamine (6-OHDA) rat model has been used to study neurodegeneration in the nigro-striatal dopaminergic system. The goal of this study was to evaluate the reliability of diffusion MRI and resting-state functional MRI biomarkers in monitoring neurodegeneration in the 6-OHDA rat model assessed by quantitative histology.</p> <p>We performed a unilateral injection of 6-OHDA in the striatum of Sprague Dawley rats to produce retrograde degeneration of the dopamine neurons in the substantia nigra pars compacta. We carried out a longitudinal study with a multi-modal approach combining structural and functional MRI together with quantitative histological validation to follow the effects of the lesion. Functional and structural connectivity were assessed in the brain of 6-OHDA rats and sham rats (NaCl injection) at 3 and 6 weeks post-lesioning using resting-state functional MRI and diffusion-weighted.</p> <p>The shared datafile corresponds to the MRI biomarkers extracted from diffusion and functional acquisitions as well as from histological mesaurements within striatum and substantia nigria.</p>
Data for Characterization of cytokine response to intraperitoneally administered LPS & subdiaphragmatic branch vagus nerve stimulation in rat model
<p>These are the data files used for publication "Characterization of cytokine response to intraperitoneally administered LPS & subdiaphragmatic branch vagus nerve stimulation in rat model" to be published in PLOS ONE.</p>
Peptide Pool Instability of Precancerous Lesion in Rats with Model of Chronic Pancreatitis and/or Without Type 1 Diabetes Mellitus
<p><span><strong>Supplementary table 1. </strong>Data of Shapiro-Wilk (W) normality test and Homogeneity of Variance Test (Levene's F Test).</span></p> <p> </p> <p><span><strong>Supplementary table 2. </strong>Kruskal-Wallis as the overall test (H-values) and posthoc Dunn's test with Bonferroni correction. The corrected α using the Bonferroni correction method is 0.017</span></p>
COMSOL models for Electronic "photoreceptors" enable prosthetic vision with acuity matching the natural resolution in rats
<p>The in-vitro and in-vivo COMSOL models described in paper <em>Electronic “photoreceptors” enable prosthetic vision with acuity matching the natural resolution in rats.</em></p> <p><a href="https://zenodo.org/api/files/60e7380c-a31e-429c-a337-0a8b267d6bcc/Flat_pdish_final.mph">Flat_pdish_final.mph</a>: In-vitro model of the photovoltaic array tested in a Petri dish filled with 10x diluted saline water.</p> <p><a href="https://zenodo.org/api/files/60e7380c-a31e-429c-a337-0a8b267d6bcc/Flat_RatEye_final.mph">Flat_RatEye_final.mph</a>: In-vivo model of the photovoltaic array implanted in the subretinal space of an RCS rat. The rat eye is anatomically realistic, while the head is replaced by a cylinder for computational tractability.</p> <p> </p>
Estradiol Enhances the Development of Addition-Like Features in a Female Rat Model of Opioid Use Disorder
<p>This dataset indicates that, as with findings with psychostimulants and alcohol, estradiol enhances vulnerability in females to developing opioid addiction-like features and serious opioid-related health complications in a rat model of opioid use disorder.</p>
ARRIVE checklist for 'The role of proteomics analysis in carcinogenesis in a rat mammary cancer model induced by DMBA (7,12-Dimethylbenz[a]anthracene)
<p>The ARRIVE guidelines for animal reporting: ‘The role of proteomics analysis in carcinogenesis in a rat mammary cancer model induced by DMBA (7,12-Dimethylbenz[a]anthracene)’</p>
Evaluation of in vitro rat and human airway epithelial models for acute inhalation toxicity testing
<p><em>In vivo</em> models (mostly rodents) are currently accepted by regulatory authorities for assessing acute inhalation toxicity. Considerable efforts have been made in recent years to evaluate in vitro human airway epithelial models (HAEM) as replacements for <em>in vivo</em> testing. In the current work, an organotypic <em>in vitro</em> rat airway epithelial model (RAEM), rat EpiAirway™, was developed and characterized to allow a direct comparison with the available HAEM, human EpiAirway™, in order to address potential interspecies variability in responses to harmful agents. The rat and human models were evaluated in two independent laboratories with 14 reference chemicals, selected to cover a broad range of chemical structures and reactive groups, as well as known acute animal and human toxicity responses, in three replicate rounds of experiments. Toxicity endpoints included changes in tissue viability (MTT assay), epithelial barrier integrity (TEER, transepithelial electrical resistance), and tissue morphology (histopathology). The newly developed rat EpiAirway™ model produced reproducible results across all replicate experiments in both testing laboratories. Furthermore, a high level of concordance was observed between the RAEM and HAEM toxicity responses (determined by IC<sub>25</sub>) in both laboratories, with R<sup>2</sup> = 0.78 and 0.88 when analyzed by TEER; and R<sup>2</sup> = 0.92 for both when analyzed by MTT. These results indicate that rat and human airway epithelial tissues respond similarly to acute exposures to chemicals. The new in vitro RAEM will help extrapolate to<em> in vivo</em> rat toxicity responses and support screening as part of a 3Rs program.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.