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FIGURE 2. Spinopyrgus luismaedai n. gen. et n in A new freshwater snail genus and species (Gastropoda: Caenogastropoda, Cochliopidae) with extremely spinous shells from sub-recent spring deposits in northeastern Mexico

FIGURE 2. Spinopyrgus luismaedai n. gen. et n. sp. (A–K). A–B. Holotype (UJMC-250) from Juan Guerra spring. C. Paratype 1 (UJMC-251) from Juan Guerra spring. D. Paratype 2 (UJMC-252) from Juan Guerra spring. E, F. Paratype 3 (UJMC-252a) from Juan Guerra spring, specimen with three keels. G. Specimen (UJMC-253) from El Molino spring. H. Specimen (UJMC- 254) from Juan Guerra spring. I. Specimen (UJMC-253) from El Molino spring, protoconch view. J. Paratype 3 (UJMC-251), details of the carina with spines. K. Specimen (UJMC-253b) from El Molino spring, details of the carina with shovel-shaped spines.

FIGURE 1 in A new freshwater snail genus and species (Gastropoda: Caenogastropoda, Cochliopidae) with extremely spinous shells from sub-recent spring deposits in northeastern Mexico

FIGURE 1. Study area showing the locations of the Viesca springs. The scale bar refers to the map with the springs marked. The topography is illustrated using HillShade in ArcGIS.

Figure 11 in Evolutionary systematics of the viviparous gastropod Sermyla (Gastropoda: Cerithioidea: Thiaridae), with the description of a new species

Figure 11. Anatomy of the radula in Sermyla. A, rachis and lateralia of Sermyla kupaensis (ZMB 191388). B, marginalia of Sermyla kupaensis (ZMB 191388). C, rachis and lateralia of S. riquetii (BMNH 2403). D, marginalia of S. riquetii (BMNH 2403). E, rachis and lateralia of S. carbonata (ZMB 106700). F, marginalia of S. carbonata (ZMB 106700).

Figure 10 in Evolutionary systematics of the viviparous gastropod Sermyla (Gastropoda: Cerithioidea: Thiaridae), with the description of a new species

Figure 10. Non-metric dimensional scaling (NMDS) on the complete AFLP dataset. Coloured symbols indicate the same cluster; black symbols indicate that a direct cluster affiliation is not possible for that individual. Note for S. carbonata: all colored asterisks are the same cluster.

Figure 7 in Evolutionary systematics of the viviparous gastropod Sermyla (Gastropoda: Cerithioidea: Thiaridae), with the description of a new species

Figure 7. Neighbor-net analysis of AFLP data based on five primer combinations and a matrix corrected by AMARE; coloration of Sermyla kupaensis, S. riquetii and S. carbonata according to their sampling locations and river systems, additional colors represent additional thiarid species. Only one individual had a mixed genotype and was not clearly associated with one of the clusters.

Figure 8 in Evolutionary systematics of the viviparous gastropod Sermyla (Gastropoda: Cerithioidea: Thiaridae), with the description of a new species

Figure 8. Structure analysis of AFLP data based on five primer combinations and a matrix corrected by AMARE. A, structure analysis of the AFLP dataset under the assumption of admixture (limited gene flow). B, structure analysis of the AFLP dataset under the assumption without admixture (no gene flow).

Figure 5 in Evolutionary systematics of the viviparous gastropod Sermyla (Gastropoda: Cerithioidea: Thiaridae), with the description of a new species

Figure 5. Morphological differences of Sermyla kupaensis, S.riquetii and S. carbonata populations. A, boxplots for the height measurements of Sermyla kupaensis, S.riquetii and S. carbonata from the different sampling locations and river systems. Five-pointed star: syntypes of S. riquetii; six-pointed stars: syntypes of M. venustula; triangle tip down: holotype of S. prognata; nine-pointed star: syntypes of S. carbonata. B, boxplots for the total amount of juveniles inside the brood pouch of S.riquetii and S. carbonata from different sampling locations and drainage systems, as well as a veliger larva from Sermyla kupaensis for comparison.

Figure 6. Concatenated COI and 16S in Evolutionary systematics of the viviparous gastropod Sermyla (Gastropoda: Cerithioidea: Thiaridae), with the description of a new species

Figure 6. Concatenated COI and 16S mtDNA-phylogeny of Sermyla kupaensis, S. riquetii and S. carbonata and other Australian thiarid species, as well as two Paludomus siamensis as outgroup, with numbers at nodes refering to maximum likelihood bootstrap values (left) and Bayesian posterior probabilities (right). Symbols along branches indicate the mode of reproduction: squares = euviviparous; circles = ovoviviparous. Abbreviations refer to sampling locations: HS = Howard Springs; GC = Gulf of Carpentaria; RR = Roper River; WA = Western Australia (Bundara Sinkhole); BAL = Bali; VIE = Vietnam; THA = Thailand.

Figure 9 in Evolutionary systematics of the viviparous gastropod Sermyla (Gastropoda: Cerithioidea: Thiaridae), with the description of a new species

Figure 9. Structure analysis of AFLP data based on five primer combinations and a matrix corrected by AMARE for the reduced dataset of S. carbonata in Australia. Results have been assigned to their geographical localities.

Figure 2 in Evolutionary systematics of the viviparous gastropod Sermyla (Gastropoda: Cerithioidea: Thiaridae), with the description of a new species

Figure 2. Geographic range of S. riquetii (circles), S. kupaensis (hexagon) and S. carbonata (squares) with a colour-code for the different drainage systems in Australia. Black squares: sequenced, ethanol preserved material of S. carbonata; white squares: dry shells of S. carbonata; black circles: sequenced, ethanol preserved material of S. riquetii; white circles: dry shells of S. riquetii; black hexagon: sequenced, ethanol-preserved material of S. kupaensis; white hexagon: dry shells of S. kupaensis; five-pointed stars: type localities of 1 = S. riquetii, 2 = S. kupaensis, 3 = S. carbonata, 4 = M. venustula.

Figure 3 in Evolutionary systematics of the viviparous gastropod Sermyla (Gastropoda: Cerithioidea: Thiaridae), with the description of a new species

Figure 3. Shell measurements and positions of landmarks for morphological analyses of the shell of Sermyla species. A, shell measurements for biometrical analysis: ShH, shell height; L3WH, height of the last three whorls; LWH, last whorl height; AW, width of the aperture; AH, height of the aperture; ShW, width of the shell. B, landmarks set for the geometric morphometric analysis.

Figure 4 in Evolutionary systematics of the viviparous gastropod Sermyla (Gastropoda: Cerithioidea: Thiaridae), with the description of a new species

Figure 4. Shape differences of populations of S. riquetii, S. carbonata and S. kupaensis. Principle component analysis (PCA) for the configuration of 15 landmarks of S. kupaensis from Sulawesi, S. riquetii from Bali, Vietnam and Thailand, S. cf. riquetii from Australia, as well as S. carbonata in the different drainage systems of Australia. Five-pointed star: syntype of S. riquetii; nine-pointed star: syntypes of S. carbonata; six-pointed stars: syntypes of M. venustula; triangle tip down: holotype of S. prognata.

Figure 1. A in Evolutionary systematics of the viviparous gastropod Sermyla (Gastropoda: Cerithioidea: Thiaridae), with the description of a new species

Figure 1. A–AB, shell morphology of Sermyla: A–O, Sermyla riquetii; P–T, Sermyla kupaensis; U–AB, Sermyla carbonata. A, syntype of Sermyla riquetii (Grateloupe, 1840), 'Batavia' (BMNH 1907.11.22.40); B, syntype of Sermyla riquetii (Grateloupe, 1840), 'Batavia' (BMNH 1907.11.22.41); C, syntype of Melania harpula Dunker, 1844 (ZMB 109669); D, syntype of Melania mitra Dunker, 1844 (ZMB 109670); E, original drawing of Melania semicostata Philippi 1847; F, syntype of Melania tornatella Lea & Lea, 1851 (BMNH 1978155); G, syntype of Melania sculpta Souleyet, 1852 (BMNH 1854.7.24.381); H, holotype of Sermyla kowloonensis Chen, 1943 (USNM 48041); I, S. riquetii from Bali, Gumbrih River (ZMB 106474-3); J, S. riquetii from Thailand, Puek Tian Beach (ZMB 107883-11); K, S. riquetii from Thailand, Perchaburi (ZMB 127817-2); L, S. riquetii from Thailand, Samut Songkhram (ZMB 127818-2); M, S. riquetii from Vietnam, Hue (ZMB 114421–5); N, S. cf. riquetii from Australia: QLD, Caloundra (AMS C.3215-1); O, S. cf. riquetii from Australia: QLD, Caloundra (AMS C.3215-2); P, holotype of Sermyla kupaensis from Indonesia, Kupa River (MZB Gst. 12.191); Q, paratype of Sermyla kupaensis ZMB 191388-4); R, paratype of Sermyla kupaensis (ZMB 191388-10); S, paratype of Sermyla kupaensis (ZMB 191388-5); T, paratype of Sermyla kupaensis (ZMB 191388-7); U, syntype of Sermyla carbonata (Reeve, 1859), 'Port Essington' (BMNH 2001.0762); V, syntype of Melania venustula Brot, 1877, 'Port Denison' (MNHG, no number); W, holotype of Sermylasma prognata Iredale, 1943, Australia, Victoria River (BMNH 1857.9.30.8-1); X, S. carbonata, Australia: Bundara Sinkhole (BES 10048); Y, S. carbonata, Australia, Howard Springs (ZMB 107630–2); Z, S. carbonata, Australia, Roper River (ZMB 107616-1); AA, S. carbonata, Australia, Roper Bar (ZMB 192017-1); AB, S. carbonata, Australia, Norman River (107209-5).

Figure 10 in Biodiversity and phylogeny of Cocculinidae (Gastropoda: Cocculinida) in the Indo-West Pacific

Figure 10. Protoconchs of sequenced specimens: A, MNHN-IM-2013-42570 (MD3); B, MNHN-IM-2013-42648 (MD4); C, MNHN-IM-2013-62341 (MD5); D, MNHN-IM-2013-62343 (MD8); E, MNHN-IM-2013-42654 (ME1); F, MNHN-IM-2013-42627 (MF9); G, MNHN-IM-2013-42878 (MF6); H, MNHN-IM-2013-42748 (MF2); I, MNHN-IM-2013-42907 (MG13); J, MNHN-IM-2013-42798 (MG8); K, MNHN-IM-2013-42896 (MG5); L, MNHN-IM-2009-11985 (MH1). Scale bars = 20 µm.

Figure 3 in Biodiversity and phylogeny of Cocculinidae (Gastropoda: Cocculinida) in the Indo-West Pacific

Figure 3. Characters used in morphological examination. Teleoconch structure: A, radially ribbed (MNHN-IM-2013-42733, MC10), B, radially ribbed with pits and hairs (MNHN-IM-2013-42896, MG5), C, plainly raised radially ribbed (MNHN-IM-2013-62346, MB7), D, clathrate (MNHN-IM-2013-62341, MD5), E, concentric (MNHN-IM-2009-11985, MH1) or F, smooth (MNHN-IM-2013-42654, ME1).

Figure 2 in Biodiversity and phylogeny of Cocculinidae (Gastropoda: Cocculinida) in the Indo-West Pacific

Figure 2. Diagram of the integrative approach used for species delimitation. ABGD: Automatic Barcode Gap Discovery; PTP: Poisson Tree Processes.

Figure 7 in Biodiversity and phylogeny of Cocculinidae (Gastropoda: Cocculinida) in the Indo-West Pacific

Figure 7. Nonmetric multidimensional scaling (NMDS) plot based on the Jaccard index for cocculinid species composition among 157 stations in the Indo-West Pacific.

Figure 1 in Biodiversity and phylogeny of Cocculinidae (Gastropoda: Cocculinida) in the Indo-West Pacific

Figure 1. Localities of cocculinid specimens collected during our expeditions (red) and those in literature records (yellow; Watson, 1886; Schepman, 1908; Thiele, 1925; Marshall, 1985; Hasegawa, 1997; Lesicki, 1998; Zhang & Zhang, 2018) in the Indo-West Pacific. Squares indicate the 11 regions identified. AFR: Mozambique Channel; MAD: Madagascar; TW: Taiwan plus South China Sea; ECS: East China Sea; PHI: seas surrounding the Philippines; JP: seas surrounding Japan; BIS: Bismarck Sea; SOL: Solomon Sea; COR: Coral Sea; VAN: seas surrounding Vanuatu; NC: seas surrounding New Caledonia.

Figure 11 in Biodiversity and phylogeny of Cocculinidae (Gastropoda: Cocculinida) in the Indo-West Pacific

Figure 11. Radulae of sequenced specimens and an additional examined specimen of C. japonica: A, MNHN-IM-2013-42671 (MA1), 'unicuspid broad'; B, MNHN-IM-2013-42687 (MA4), 'unicuspid broad'; C, MNHN-IM-2013-62344 (MA5), 'multicuspid broad'; D, MNHN-IM-2013-42828 (MA12), 'unicuspid broad'; E, MNHN-IM-2013-42715 (MB1), 'unicuspid narrow'; F, MNHN-IM-2013-42845 (MB10), 'unicuspid narrow'; G, MNHN-IM-2013-42803 (MC14), 'unicuspid broad'; H, C. japonica, 'obsolete'; I, MNHN-IM-2013-42799 (MC18), 'acuspate-flat'; J, MNHN-IM-2013-42968 (MC16), 'unicuspid broad'; K, MNHN-IM-2013-42608 (MC9), 'acuspate-flat'; L, HS217 (MC6), 'acuspate-flat'; M, MNHN-IM-2013-42570 (MD3), 'multicuspid narrow'; N, MNHN-IM-2013-42643 (MD8), 'multicuspid narrow'; O, MNHN-IM-2013-42620 (ME2),

Figure 8 in Biodiversity and phylogeny of Cocculinidae (Gastropoda: Cocculinida) in the Indo-West Pacific

Figure 8. Combined three-gene ML tree for 138 cocculinid specimens (1421 bp from cox1, 28S and H3). Nodal supports are shown as circles on nodes. Nodes supported by both bootstrap value (BS) ≥ 80% and posterior probability (PP) of ≥ 0.95 are shown in black circles; nodes supported by only BS ≥ 80% or PP ≥ 0.95 are shown in grey circles. Nodes with BS of <80% and PP <0.95 are not marked with a circle. Morphogroup types are plotted on the tree (Supporting Information, Table S2). Geographic distribution of each species is shown with filled square(s). Different shapes of rachidian tooth of radula are denoted with following symbols: X, obsolete;—, acuspate; circle with three spikes, multicuspid; circle with a single spike, unicuspid. Dotted squares, broad or narrow, indicate relative width of rachidian tooth. Asterisks represent nonmonophyletic morphogroups.