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MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 3 3323 V1.0

This is a Mars Express Radio Science data set, collected during the extended mission phase 2010-01-01 to 2012-12-31. It is a Global Gravity measurement and covers the time 2012-08-05T03:31:55.000 to 2012-08-05T05:52:22.500.

Transcriptional profiling of thymus from mice flown on the RR-9 mission

The objective of the Rodent Research-9 (RR-9) mission was to use mice to understand the molecular basis of phenomena that affect astronauts during long-duration spaceflight, particularly visual impairment, and joint tissue degradation. To this end, a flight group (FLT) of 10-week-old male C57BL/6J mice were launched from Kennedy Space Center (KSC) on 8/14/2017 and housed in Rodent Habitats on the ISS for 33 days before being returned alive to Earth. After splashdown in the Pacific Ocean, the animals were transported to Loma Linda University (LLU) for testing, euthanasia, and dissection on 9/18/2018. Ground Control (GC) studies were planned to commence at KSC approximately one-week after the conclusion of the flight experiments. However, all the GC mouse studies at KSC had to be cancelled due to Hurricane Irma and potential adverse effects on the animal housing facility. The GC studies were therefore rescheduled and begun in May 2018. The GC was euthanized and dissected 6/18/2018 - 6/20/2018. Because this resulted in a different cohort of mice being used for the GC controls as compared to the flight (FLT) groups, two cohort controls were included in the study. The first, Cohort Control 1 (CC_C1), was from the same cohort as the FLT animals and was sacrificed and dissected 4 days after the FLT group (9/22/2017). The second, Cohort Control 2 (CC_C2), was from the same cohort as the GC, and was sacrificed and dissected 2-8 days after the GC, (6/24/2018 - 6/26/2018). The CC_C1 and CC_C2 groups were housed in standard cages and fed standard chow in contrast to all other groups which received Rodent Foodbars. Upon dissection, thymus tissues were preserved in liquid nitrogen and stored at 80 C before RNA was extracted. Only the flight (FLT, n of 10) and Ground Control (GC, n of 10) samples were processed and analyzed in this study. Libraries were generated using a 3’ Tag-seq approach and sequenced at a targeted depth of 40 M clusters (SE 93 bp).

Bulk RNA sequencing and spatially resolved transcriptional profiling of cerebellums from mice flown on the RR-10 mission

The objective of the Rodent Research-10 mission (RR-10) was to investigate how spaceflight affects the cellular and molecular mechanisms of normal bone tissue regeneration in space. To this end, ten (10) 14-15 weeks-old female B6129SF2/J Wild Type (WT), and ten (10) 14-15 weeks-old female B6;129S2-Cdkn1atm1Tyj/J (p21-null) mice received a pre-flight subcutaneous injection of the bone marker (Alizarin Red), and were then delivered to the ISS aboard SpaceX-21. At 7 days before euthanasia, all 20 mice received an intraperitoneal (IP) injection with a bone formation marker (Calcein). At 48 +/- 2 hours before euthanasia, all 20 mice received an IP injection with a second dose of Calcein as well as a cell proliferation marker (BrdU). Then, following 28-29 days in microgravity, the Flight mice were euthanized. Following removal of hindlimbs, carcasses were wrapped in aluminum foil, preserved in the CryoChiller, and stored at -80 C or colder until return to Earth. In addition to the Flight group, three ground control groups were also part of the study: Basal (representing the pre-launch state), Vivarium (standard vivarium housing for the same duration of time as flight), and Ground (flight habitat in the International Space Station Environment Simulator, ISSES). Twenty mice (10 of each strain) were included in each of these control groups (except Vivarium which included 12 of each strain). These were treated, euthanized and processed on the same schedule and in the same manner as the flight samples. This study includes bulk RNA sequencing and spatially resolved transcriptional profiling data from cerebellums from 5 WT flight animals, and 5 WT ground control animals. Cerebellums from the right hemisphere were embedded and cryosectioned. Cryosections were either processed for bulk RNA sequencing or placed on gene expression arrays, stained and imaged. Imaging was followed by tissue permeabilization to release mRNA molecules from cells for capture onto the array surface. Subsequently, spatial transcriptomics libraries were prepared and sequenced.

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 5 3828 V1.0

This is a Mars Express Radio Science data set, collected during the extended mission phase 2015-01-01 to 2016-12-31. It is a Occultation measurement and covers the time 2015-09-23T12:52:46.000 to 2015-09-23T13:16:38.949.

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 3 3348 V1.0

Transcriptional analysis of spleen from mice flown on the RR-6 mission

The objective of the Rodent Research-6 (RR-6) study was to evaluate muscle atrophy in mice during spaceflight and to test the efficacy of a novel therapeutic to mitigate muscle wasting. The experiment involved an implantable subcutaneous nanochannel delivery system (nDS; between scapula), which delivered the drug formoterol (FMT; a selective Beta-2 adrenoceptor agonist), over the course of time. To this end, a cohort of forty 32-weeks-old female C57BL/6NTac mice were either sham operated or implanted with vehicle or treatment-filled nDS, launched in two Transporters (20 mice per Transporter) on SpaceX-13 on December 15, 2017. They were transferred to Rodent Habitats onboard the International Space Station (ISS), and maintained in microgravity for 29 days (N of 20, Live Animal Return [LAR]), or >50 days (N of 20, ISS Terminal). After 29 days, the 20 LAR animals were returned live to back to Earth on January 13, 2018,. After splashdown, the animals were ambulatory on-ground for ~4 days, until all subjects were processed during one day of dissections. There were two Baseline groups of animals sacrificed (LAR Baseline & FLT Baseline; N of 20; 40 animals; ~36 weeks old) at Kennedy Space Center (KSC; 12/9/17). A Ground Control group mimicked the Flight LAR group, which was housed at KSC, then shipped alive, to Novartis' Facilities, where both the LAR and LAR Ground Control groups were processed (~41 weeks old; 1/16/18). All were anesthetized with isoflurane, blood samples were obtained by closed-chest cardiac puncture, and the animals were euthanized by exsanguination and thoracotomy. The 20 ISS Terminal mice were anesthetized via intraperitoneal injection of ketamine/xylazine/acepromazine over the course of a four days of dissections (2/6/18 until 2/9/18; 53-56 days after launch; 44 weeks old at time of on-orbit dissections). Blood samples and euthanasia were conducted the same as LAR and Baseline. Following blood draw and hind limb dissection, the ISS-terminal animal carcasses were wrapped in aluminum foil, placed in a ziploc bag and placed in storage at -80C or colder until return. The ISS-terminal Ground Controls (at KSC) followed the same euthanasia timeline, methods, and preservation. The final processing of frozen ISS-terminal, frozen ISS-terminal Ground Controls and frozen 0-day FLT baseline animals were completed at Houston Methodist Research Institute, in Houston, TX (5/21/18 until 5/24/18). This study features data from only sham treated animals (no drug treated animals) from the following groups Flight: LAR (N of 8), ISS Terminal (N of 7); Ground Controls: LAR (N of 9), ISS Terminal (N of 7); Baseline: LAR (N of 10), ISS Terminal (N of 3). Total RNA was extracted and sequenced at a target depth of 60 M clusters per sample (ribodepleted, paired end 150).

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 5 3801 V1.0

This is a Mars Express Radio Science data set, collected during the extended mission phase 2015-01-01 to 2016-12-31. It is a Occultation measurement and covers the time 2015-08-28T14:42:39.050 to 2015-08-28T15:06:32.949.

MARS EXPRESS SUN MRS 1/2/3 EXTENDED MISSION 3 2765 V1.0

This is a Mars Express Radio Science data set, collected during the extended mission phase 2010-01-01 to 2012-12-31. It is a Solar Conjunction measurement and covers the time 2011-02-09T14:00:15 to 2011-02-09T16:29:57.500.

MARS EXPRESS SUN MRS 1/2/3 EXTENDED MISSION 3 2767 V1.0

37-Day microgravity exposure in 16-Week female C57BL/6J mice during the NASA Rodent Research 1 mission is associated with bone loss specific to weight-bearing skeletal sites (femur and vertebrae, micro computed tomography)

Exposure to weightlessness in microgravity and elevated space radiation are associated with rapid bone loss in mammals, but questions remain about their mechanisms of action and relative importance. In this study, we tested the hypothesis that bone loss during spaceflight in Low Earth Orbit is primarily associated with site-specific microgravity unloading of weight-bearing sites in the skeleton. Microcomputed tomography and histological analyses of bones from mice space flown on ISS for 37 days in the NASA Rodent Research-1 experiment show significant site-specific cancellous and cortical bone loss occurring in the femur, but not in L2 vertebrae. The lack of bone degenerative effects in the spine in combination with same-animal paired losses in the femur suggests that space radiation levels in Low Earth Orbit or other systemic stresses are not likely to significantly contribute to the observed bone loss. Remarkably, spaceflight is also associated with accelerated progression of femoral head endochondral ossification. This suggests the microgravity environment promotes premature progression of secondary ossification during late stages of skeletal maturation at 21 weeks. Furthermore, mice housed in the NASA ISS Rodent Habitat during 1g ground controls maintained or gained bone relative to mice housed in standard vivarium cages that showed significant bone mass declines. These findings suggest that housing in the Rodent Habitat with greater topological enrichment from 3D wire-mesh surfaces may promote increased mechanical loading of weight-bearing bones and maintenance of bone mass. In summary, our results indicate that in female mice approaching skeletal maturity, mechanical unloading of weight-bearing sites is the major cause of bone loss in microgravity, while sites loaded predominantly by muscle activity, such as the spine, appear unaffected. Additionally, we identified early-onset of femoral head epiphyseal plate secondary ossification as a novel spaceflight skeletal unloading effect that may lead to premature long bone growth arrest in microgravity. This study derives results from femur and vertebrae using the micro computed tomography assay.

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 3 3300 V1.0

Transcriptional profiling of right extensor digitorum longus muscle from mice flown on the RR-23 mission

The objective of the Rodent Research-23 mission (RR-23) was to better understand the effects of spaceflight on the eyes, specifically on the structure and function of the arteries, veins, and lymphatic vessels that are needed to maintain vision. To this end, twenty male, C57BL/6J, 16-17 weeks-old mice were delivered to the International Space Station (ISS) on SpaceX-21 in a single transporter, transferred to two rodent habitats, and maintained in microgravity for 38 days. Flight mice were then returned to Earth alive (January 13th, 2021). After splashdown in the Atlantic Ocean, mice were transported to Kennedy Space Center via helicopter. The 20 Flight, 20 Habitat Ground Control (HGC), and 20 Vivarium Ground Control (VGC) mice were removed from Rodent Transporters (Flight and HGC) or vivarium cages (VGC), placed into shipping containers, and flown to Texas A and M University. There, mice underwent post-flight procedures, before euthanasia and tissue collection. Flight, HGC and VGC animals were euthanized and dissected on Jan 14th, 17th or 20th of 2021, respectively. Right extensor digitorum longus muscle samples were preserved by immersion in liquid nitrogen and stored at -80C until RNA was extracted, and libraries generated and sequenced (target 60 M clusters per sample, PE 150 bp). This dataset features 9 samples from the Flight group, 9 samples from the Habitat Ground Control group, and 9 samples from the Vivarium Ground Control group.

GIOTTO EXTENDED MISSION DUST IMPACT DETECTOR V1.0

This dataset contains the results obtained from the Dust Impact Detection System (DIDSY) flown aboard the GIOTTO spacecraft during its extended mission to Comet P/Grigg-Skjellerup in 1992. The measurements and analysis of the three impacts detected during close approach are contained in text files. Calibration tables for the sensors are included in this dataset. These data have not been reviewed or formally archived with the PDS.

restrictednotspecifiedMar 2025View details →

A Modeling Framework for Prognostic Decision Making and its Application to UAV Mission Planning

The goal of prognostic decision making (PDM) is to utilize information on anticipated system health changes in selecting future actions. One of the key challenges in PDM is finding a sufficiently expressive yet compact mathematical representation of the system for use with decision optimization algorithms. In this paper we describe a general modeling approach for a class of PDM problems with non-linear system degradation processes and uncertainties in state estimation, action effects, and future operating conditions. The approach is based on continuous Partially Observable Markov Decision Processes (POMDPs) used in conjunction with 'black box' system simulations. The proposed modeling framework can be cast into simpler representations, depending on which sources of uncertainty are being included. The approach is illustrated with a mission planning case study for an unmanned aerial vehicle (UAV). In the case study a PDM system is tasked with optimizing the vehicle route after an in-flight component fault is detected. A stochastic algorithm (based on particle filtering) is used for decision optimization, with a second, deterministic algorithm providing a performance evaluation baseline. Both algorithms utilize a UAV physics simulator for generating predictions of future vehicle states. Performance benchmarking is done on a set of mission scenarios of increasing complexity.

Transcriptional profiling of the right gastrocnemius muscle from mice flown on the RR-1 mission

In the NASA Rodent Research-1 (RR-1) validation study, ten 16-week-old female C57BL/6J mice were flown to the ISS for 37 days before euthanasia and subsequent dissection (Flight group). Due to crew time constraint, only two (out of ten) mice were dissected immediately after euthanasia to recover spleen and liver tissues on the ISS. The remaining eight animals were euthanized, then intact carcasses were placed in a pre-chilled cold stowage container and stored in the MELFI. There were respective cohorts of age-matched basal animals which were euthanized one day after launch as a baseline control (Basal control group) as well as age-matched ground control animals kept in an ISS Environmental Simulator at Kennedy Space Center (KSC) on a 4-day delay to mimic spaceflight conditions (Ground control group). In addition, the NASA Validation study also had a cohort of age-matched vivarium control animals that were housed in the vivarium cages and followed the same experimental timeline and process as the spaceflight animals (Vivarium control group). This dataset was generated only from Flight (n of 4) and Ground control (n of 8) animals that were euthanized and preserved intact on-orbit for subsequent dissection on the ground. Libraries were generated using a 3’ Tag-seq approach and sequenced at a depth of 40 M clusters (SE 93 bp).