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opennotspecifiedAug 2023View details →

FIGURE 3. Peliosanthes linearifolia, floral morphology. A in Peliosanthes linearifolia (Asparagaceae), a new species with linear leaves from Vietnam

FIGURE 3. Peliosanthes linearifolia, floral morphology. A. Flower (lateral view). B, C. Flowers at the very beginning anthesis and at late anthesis (front views). D, E. Flowers (semi-side views). F. Corona, anthers and style (front view). G, H. Longitudinal sections of flowers. Nuraliev, Lyskov NUR 3457 (B–D, H) and Nuraliev, Lyskov NUR 3463 (A, E–G). Photos by M.S. Nuraliev.

Fig. 7. Linear correlation between experimental and calculated 13C in Discovery of Undescribed Monoterpenoid Polyprenylated Acylphloroglucinols with Immunosuppressive Activities from Hypericum longistylum

Fig. 7. Linear correlation between experimental and calculated 13C NMR chemical shifts of 5 at the B972/pcSseg-2 (A) and mPW1PW91/def2-TZVP (B) levels.

Fig. 5. Linear correlation between experimental and calculated 13C in Discovery of Undescribed Monoterpenoid Polyprenylated Acylphloroglucinols with Immunosuppressive Activities from Hypericum longistylum

Fig. 5. Linear correlation between experimental and calculated 13C NMR chemical shifts of 3 at the B972/pcSseg-2 (A) and mPW1PW91/def2-TZVP (B) levels.

Fig. 3. Linear correlation between experimental and calculated 13C in Discovery of Undescribed Monoterpenoid Polyprenylated Acylphloroglucinols with Immunosuppressive Activities from Hypericum longistylum

Fig. 3. Linear correlation between experimental and calculated 13C NMR chemical shifts of 1 at the B972/pcSseg-2 (A) and mPW1PW91/def2-TZVP (B) levels.

Fig. 1 in Rapid screening of glycosyltransferases in plants using a linear DNA expression template based cell-free transcription-translation system

Fig. 1. Rapid screening of plant glycosyltransferases using the LET-based-TX-TL system. We can either use long primers which contain a promoter, a ribosome binding site, and a terminator to generate expressible linear DNAs or use short primers to amplify the targeted gene fragments and then ligate them with a promoter, a ribosome binding site, a terminator, and a backbone; then another pair of primers is used to generate expressible linear DNAs. Afterward, combine TX-TL extracts, buffers, and expressible linear DNAs to start protein expression. Then this TX-TL mixture is directly added with substrates (such as quercetin) to start glycosylation reactions. Finally, UPLC-MS is used to analyze the reaction mixture to examine whether targeted products (such as isoquercitrin) are generated.

Fig. 2 in Rapid screening of glycosyltransferases in plants using a linear DNA expression template based cell-free transcription-translation system

Fig. 2. UPLC-MS analysis of isoquercitrin converted from quercetin by AtUGTs expressed in TX-TL. (A) A glycosylation reaction catalyzed by a UDP-glucose glycosyltransferase. (B) The chromatogram of the blank sample, which only has 50% methanol solvent. (C) The chromatogram of the negative group, which has TX-TL, quercetin, UDPglucose but no additional DNA. (D) The chromatogram of the quercetin standard. (E) The chromatogram of the isoquercitrin standard. (F–O) Chromatograms of products from the catalysis of quercetin by different AtUGTs (the final concentrations of the linear DNAs used for each AtUGTs are listed below in brackets): (F) AT1G07250 (32.25 nM), (G) AT1G07260 (29.24 nM), (H) AT2G36790 (20.64 nM), (I) AT2G15480 (29.98 nM), (J) AT2G15490 (29.50 nM), (K) AT3G16520 (25.03 nM), (L) AT3G21750 (23.7 nM), (M) AT3G46660 (24.14 nM), (N) AT4G15280 (24.47 nM), and (O) AT4G34138 (22.52 nM), see Supplementary Fig. S4 for the duplicate group. The y axis indicates ion signal abundance relative to the highest signal in each chromatogram (%). The numbers in the top right of each chromatogram (for instance 3.38e7 in [E]) indicate the ion counts represented by "100" relative abundance. The retention time of the isoquercitrin standard is 4.0 min, and the red arrow in each figure indicates the retention time of the product isoquercitrin. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 4 in Rapid screening of glycosyltransferases in plants using a linear DNA expression template based cell-free transcription-translation system

Fig. 4. UPLC-MS analysis of isoquercitrin converted from quercetin by ArUGTs using heterologous expression. (A) The SDS-PAGE gel of heterologous expression of ArUGTs AR14572, AR11662, and AR43718 protein. The theoretical molecular weights of AR14572 protein, AR11662 protein, and AR43718 protein are 80.1 kDa, 79.8 kDa, and 80.4 kDa, respectively. (B) The chromatogram of isoquercitrin standard. (C–E) Chromatograms of products from the catalysis of quercetin by different ArUGTs using heterologous expression: (C) AR14572, (D) AR11662, and (E) AR43718, see Supplementary Fig. S6 for the duplicate group. The y axis indicates ion signal abundance relative to the highest signal in each chromatogram (%). The numbers in the top right of each chromatogram (for instance 4.56e6 in [B]) indicate the ion counts represented by "100" relative abundance. The retention time of the isoquercitrin standard is 3.93 min, and the red arrow in each figure indicates the retention time of the product isoquercitrin. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 5. A in Rapid screening of glycosyltransferases in plants using a linear DNA expression template based cell-free transcription-translation system

Fig. 5. A phylogenetic tree based on protein sequences of six ArUGTs: AR06047, AR06981, AR07558, AR11662, AR14572, and AR43718. The phylogenetic tree is constructed using MEGA. Numbers at the forks are bootstrap values from 100 replicates.

Fig. 3 in Rapid screening of glycosyltransferases in plants using a linear DNA expression template based cell-free transcription-translation system

Fig. 3. UPLC-MS analysis of isoquercitrin converted from quercetin by ArUGTs expressed in TX-TL. (A) A glycosylation reaction catalyzed by a UDP-glucose glycosyltransferase. (B) The chromatogram of the blank sample is 50% methanol solvent. (C) The chromatogram of the negative group, which has TXTL, quercetin, UDP-glucose but no additional DNA. (D) The chromatogram of quercetin standard. (E) The chromatogram of isoquercitrin standard. (F–K) Chromatograms of products from the catalysis of quercetin by different ArUGTs (the final concentrations of the linear DNAs used for each ArUGTs are 30 nM): (F) AR14572, (G) AR11662, (H) AR43718, (I) AR06047, (J) AR06981, and (K) AR07558, see Supplementary Fig. S5 for the duplicate group. The y axis indicates ion signal abundance relative to the highest signal in each chromatogram (%). The numbers in the top right of each chromatogram (for instance 2.21e7 in [E]) indicate the ion counts represented by "100" relative abundance. The retention time of the isoquercitrin standard is 4.08 min, and the red arrow in each figure indicates the retention time of the product isoquercitrin. The numbers under the protein names are the peak intensities of the product. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 3. Linear correlation between the experimental and calculated 13C in Two pairs of undescribed enantiomers isolated from the fungus Penicillium griseofulvum

Fig. 3. Linear correlation between the experimental and calculated 13C NMR chemical shift of 1A and 1B and their compared 13C NMR data (Δδ δ δ). = adj_calcd – expt MG857577) is 99% identical to P. griseofulvum isolate M12 (KX302025.1) and P. griseofulvum strain Yup08 (HQ262520.1).

opennotspecifiedJun 2021View details →

Fig. 4. Linear correlation between the experimental and calculated 13C in Piperazine-2,5-dione derivatives and an α-pyrone polyketide from Penicillium griseofulvum and their immunosuppression activity

Fig. 4. Linear correlation between the experimental and calculated 13C NMR chemical shift of 1-A and 1-B and their compared 13C NMR data (Δδ δ – δ).

opennotspecifiedJul 2021View details →

Fig. 5. Linear correlation between the experimental and calculated 13C NMR chemical shifts for 1A and 1B in (±)-hyperzewalsins A D, four pairs of nor-monocyclic polyprenylated acylphloroglucinols with immunosuppressive activity from hypericum przewalskii maxim

Fig. 5. Linear correlation between the experimental and calculated 13C NMR chemical shifts for 1A and 1B.

opennotspecifiedMar 2023View details →

Fig. 6. Linear correlation plots between the experimental and calculated 13C in Structurally diverse alkaloids with nine frameworks from Zephyranthes candida and their acetylcholinesterase inhibitory and anti-inflammatory activities

Fig. 6. Linear correlation plots between the experimental and calculated 13C NMR data for two isomers of 2.

opennotspecifiedMar 2023View details →

Fig. 3. Linear correlation plots between the experimental and calculated 13C in Structurally diverse alkaloids with nine frameworks from Zephyranthes candida and their acetylcholinesterase inhibitory and anti-inflammatory activities

Fig. 3. Linear correlation plots between the experimental and calculated 13C NMR data for four isomers of 1.

opencc-by-4.0Aug 2023View details →

Errors from linear performance estimation for CAIRT

Errors from linear performance estimation for CAIRT

opennotspecifiedAug 2020View details →

Fig. 3. Linear correlations between the scaled calculated and experimental 13C in Phenolics from Archidendron clypearia (Jack) I.C.Nielsen protect SH-SY5Y cells against H O -induced oxidative stress

Fig. 3. Linear correlations between the scaled calculated and experimental 13C NMR chemical shifts and statistical DP4+ parameters for compounds 1–3 (A–C).

opencc-by-4.0Sep 2023View details →

Developing a Method to Automatically Extract Road Boundary and Linear Road Markings from MMS Point Cloud using OBB Collision Detection Techniques

This video demonstrates the application of our method in a software tool for constructing road boundaries and lane marking data.

opencc-by-4.0Sep 2023View details →

Seismic data and receiver functions from two linear dense nodal array in the southern Chinese Altai region

Seismic data and receiver functions from two linear dense nodal array in the southern Chinese Altai region. All data are arranged by seismic events, and the number before the first dot of each file name represents the station number.

dryad32/100

NanoString autoimmune profiling panel normalized linear counts and summary of statistical analyses

Occupational exposure to respirable crystalline silica (cSiO2) is linked to the development of lupus. Preclinical studies have revealed weekly repeated intranasal exposure to 1 mg cSiO2 in young (8-11 wk-old) female NZBWF1 lupus-prone mice, a life-stage equivalent to 12–20-yr-old humans, triggers autoimmunity in the lungs and kidneys that is prevented by dietary supplementation with the omega-3 fatty acid docosahexaenoic acid (DHA). Methods: Here, we characterized cSiO2's and DHA's effects in mature adult (16–19-wk-old) female NZBWF1 mice, an age period that coincides with the onset of immunological tolerance breach and that is more representative of the age (>20-yr-old) of cSiO2-exposed workers. We fed mice either a control diet (CON) or diet amended with DHA calorically equivalent to a human daily dose of 5 g. After 2 wk, we intranasally instilled them with either saline vehicle (VEH) or 1 mg of cSiO2 weekly for 4 wk. Cohorts were terminated 1 and 5 wk after the final installation. Lungs were then analyzed for inflammatory cell counts, chemokines, histopathology, B-and T-cell infiltration, autoantibody profile, and inflammatory/autoimmune gene signatures and results further related to autoimmune glomerulonephritis onset. Results: VEH/CON mice displayed no lung or kidney pathology at either time point. In contrast, cSiO2/CON mice exhibited mild ectopic lymphoid tissue (ELT) formation in the lungs at 1 wk, which increased significantly by 5 wk. Lungs from cSiO2/CON mice also showed elevations in BALF cellularity, chemokine production, CD3 + T-cells, CD45R + B-cells, IgG + plasma cells, inflammatory/autoimmune gene expression, IgG autoantibodies. cSiO2/CON mice had visible glomerular hypertrophy and IgG deposition. Dietary DHA supplementation suppressed all these endpoints. Discussion: Consistent with young mice, intranasal cSiO2 exposure in mature adult NZBWF1 lupusprone mice elicited early pulmonary inflammation that served as a nexus for autoimmunity, suggesting these life-stage differences are not critical for cSiO2-triggered autoimmune response in this preclinical model. DHA supplementation at a translationally relevant human dosage effectively mitigated cSiO2-induced inflammation/autoimmunity in mature adult mice, resembling the protective effects observed in young mice. Together these findings further highlight the therapeutic potential of omega-3 fatty acids in mitigating toxicant-triggered autoimmune responses.

opencc-zeroOct 2023View details →