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Fig. 3. Haplotype network for 419 in Across mountains and ocean: species delimitation and historical connectivity in Holarctic and Arctic-Alpine wolf spiders (Lycosidae, Pardosa)

Fig. 3. Haplotype network for 419 specimens of P. saltuaria species group based on mitochondrial COI data. Haplotypes are colored based on morphological identification. Each line is 1 mutation step.

Fig. 9 in New Echinoderes (Kinorhyncha: Cyclorhagida) from Mexico: Molecular barcoding demonstrate species delimitation between highly similar morphospecies

Fig. 9. Light micrographs of cuticles from paratypes and hologenophores mounted after DNA extraction and COI sequencing. (A–B) Echinoderes horni, NHMD- 1176472, GenBank Acc. No. OP617666. (C–D) Echinoderes wilberti sp. nov., NHMD- 1176460, GenBank Acc. No. OP617672. (A) Dorsal overview. (B) Segments 1 to 6, dorsal view. (C) Dorsal overview. (D) Segments 1 to 6, dorsal view. Arrows indicate subdorsal tubes on segment 2.

Fig. 3 in New Echinoderes (Kinorhyncha: Cyclorhagida) from Mexico: Molecular barcoding demonstrate species delimitation between highly similar morphospecies

Fig. 3. Diagram of mouth cone (grey area), introvert and placids in Echinoderes abeli sp. nov., outer oral styles (diamonds), primary scalids (triangles), spinoscalids (open circles), and trichoscalids (stars), with positions of trichoscalid plates and placids indicated. Table shows the scalid arrangement by sector; single-lined boxes mark quincunxes, double-lined boxes mark "double diamonds", question mark indicates unknown information.

Fig. 1 in New Echinoderes (Kinorhyncha: Cyclorhagida) from Mexico: Molecular barcoding demonstrate species delimitation between highly similar morphospecies

Fig. 1. Maps showing (A) the position of areas of study in Mexico (B) sampling stations at Islas Marias with asterisks marking St. 8 y St.12, and (C) sampling stations in Quintana Roo with inset showing zoom of stations in Xcalak.

Fig. 6 in New Echinoderes (Kinorhyncha: Cyclorhagida) from Mexico: Molecular barcoding demonstrate species delimitation between highly similar morphospecies

Fig. 6. Line art illustrations of Echinoderes wilberti sp. nov. (A) Female, dorsal view. (B) Female, ventral view. (C) Segments 10 to 11 in male, dorsal view. (D) Segments 10 to 11 in male, ventral view. Abbreviations: lat, lateral accessory tube; ldss, laterodorsal sensory spot; ldt, laterodorsal tube; ltas, lateral terminal accessory spine; lts, lateral terminal spine; lvgco1, lateroventral glandular cell outlet type 1; lvs, lateroventral spine; lvt, lateroventral tube; mdgco1, middorsal glandular cell outlet type 1; mlss, midlateral sensory spot; pdgco1, paradorsal glandular cell outlet type 1; pe, penile spines; pvb, paraventral bristles; sdss, subdorsal sensory spot; sdt, subdorsal tube; vlss, ventrolateral sensory spot; vmgco1, ventromedial glandular cell outlet type 1; vmss, ventromedial sensory spot.

Fig. 5 in New Echinoderes (Kinorhyncha: Cyclorhagida) from Mexico: Molecular barcoding demonstrate species delimitation between highly similar morphospecies

Fig. 5. Scanning electron micrographs showing overviews and details of Echinoderes abeli sp. nov. (A) Dorsal overview. (B) Ventral overview. (C) Mouth cone, dorsal view. (D) Introvert sector 2 (paraventral). (E) Segments 1 to 2, dorsal view. (F) Segment 1 to 2, ventral view. (G) Segments 7 to 8, lateral view. (H) Segments 2 to 4, lateral view; inset shows close-up of glandular cell outlet type 2. (I) Segments 10 to 11, dorsal view, showing female sexual dimorphism. (J) Segments 10 to 11, ventral view, showing female sexual dimorphism. (K) Segments 10 to 11, laterodorsal view, showing female sexual dimorphism; inset shows close-up of laterodorsal tube. Abbreviations: ldss, laterodorsal sensory spot; ldt, laterodorsal tube; ltas, lateral terminal accessory spine; lts, lateral terminal spine; lvt, lateroventral tube; mdss, middorsal sensory spot; mlt, midlateral tube; oos, outer oral styles; pdss, paradorsal sensory spot; pr, protuberance; psp, primary spinoscalid; sdgco2, subdorsal glandular cell outlet type 2; sdss, subdorsal ss; slt, sublateral tube; sp, spinoscalid followed by introvert ring number; tr, trichoscalid; vlss, ventrolateral sensory spot; vmss, ventromedial sensory spot. Digit after abbreviation in lvt refer to segment number.

Fig. 10 in New Echinoderes (Kinorhyncha: Cyclorhagida) from Mexico: Molecular barcoding demonstrate species delimitation between highly similar morphospecies

Fig. 10. Line art illustrations of Echinoderes horni Higgins, 1983. (A) Female, dorsal view. (B) Female, ventral view. (C) Segments 10 to 11 in male, dorsal view. (D) Segments 10 to 11 in male, ventral view. Abbreviations: lat, lateral accessory tube; ldss, laterodorsal sensory spot; ldt, laterodorsal tube; ltas, lateral terminal accessory spine; lts, lateral terminal spine; lvgco1, lateroventral glandular cell outlet type 1; lvs, lateroventral spine; lvt, lateroventral tube; mdgco1, middorsal glandular cell outlet type 1; mlss, midlateral sensory spot; pdgco1, paradorsal glandular cell outlet type 1; pe, penile spines; pvb, paraventral bristles; sdss, subdorsal sensory spot; vlss, ventrolateral sensory spot; vmgco1, ventromedial glandular cell outlet type 1; vmss, ventromedial sensory spot.

Fig. 12 in New Echinoderes (Kinorhyncha: Cyclorhagida) from Mexico: Molecular barcoding demonstrate species delimitation between highly similar morphospecies

Fig. 12. Confocal laser micrographs of Echinoderes horni (NHMD-1176472) showing focal overview sections, from most dorsal in (A) and a deeper focus through (B) to (C). Sensory spots are indicated by full circles, and glandular cell outlets type 1 by dashed circles.

FIGURE 14. Hypsolebias antenori, UFPB 14900 in Molecular delimitation of the seasonal killifishes of the Hypsolebias antenori species group (Cyprinodontiformes, Rivulidae), with description of two new species from the Caatinga coastal basins, northeastern Brazil

FIGURE 14. Hypsolebias antenori, UFPB 14900, topotype, male, 34.7 mm SL: Brazil, Ceará, Limoeiro do Norte, rio Jaguaribe basin.

FIGURE 10. Bayesian inference phylogenetic reconstruction using the mitochondrial gene cox1 in Molecular delimitation of the seasonal killifishes of the Hypsolebias antenori species group (Cyprinodontiformes, Rivulidae), with description of two new species from the Caatinga coastal basins, northeastern Brazil

FIGURE 10. Bayesian inference phylogenetic reconstruction using the mitochondrial gene cox1 of the Hypsolebias antenori species-group. Vertical bars represent species complexes. Numbers next to nodes represent posterior probability values for the relevant nodes; values <0.5 are not shown.

FIGURE 6. Hypsolebias bonita new species, MZUSP 129608 in Molecular delimitation of the seasonal killifishes of the Hypsolebias antenori species group (Cyprinodontiformes, Rivulidae), with description of two new species from the Caatinga coastal basins, northeastern Brazil

FIGURE 6. Hypsolebias bonita new species, MZUSP 129608, female, paratype, 30.8 mm SL: Brazil, Rio Grande do Norte, Baraúna, Furna Feia National Park.

FIGURE 13 in Molecular delimitation of the seasonal killifishes of the Hypsolebias antenori species group (Cyprinodontiformes, Rivulidae), with description of two new species from the Caatinga coastal basins, northeastern Brazil

FIGURE 13. Land filling for civil construction projects in the type-locality of Hypsolebias gongobira and H. longignatus.

FIGURE 9 in Molecular delimitation of the seasonal killifishes of the Hypsolebias antenori species group (Cyprinodontiformes, Rivulidae), with description of two new species from the Caatinga coastal basins, northeastern Brazil

FIGURE 9. Bayesian inference based on the mitochondrial gene cox1 used for lineage delimitation of the Hypsolebias antenori species-group. Vertical bars represent the number of lineages delimited by ABGD (7), sGMYC (6), mGMYC (11), and b-PTP (10). Numbers adjacent to nodes represent posterior probabilities; values <0.50% are not shown.

FIGURE 12 in Molecular delimitation of the seasonal killifishes of the Hypsolebias antenori species group (Cyprinodontiformes, Rivulidae), with description of two new species from the Caatinga coastal basins, northeastern Brazil

FIGURE 12. Environmental impact of the duplication of the CE-40 highway in the type-locality of Hypsolebias gongobira and H. longignatus.

FIGURE 11. Hypsolebias longignatus, UFRN 5846, male 35.8 in Molecular delimitation of the seasonal killifishes of the Hypsolebias antenori species group (Cyprinodontiformes, Rivulidae), with description of two new species from the Caatinga coastal basins, northeastern Brazil

FIGURE 11. Hypsolebias longignatus, UFRN 5846, male 35.8 mm SL: Brazil, Ceará, Aquiraz, rio Pacoti basin.

FIGURE 5. Hypsolebias bonita new species, MZUSP 129608 in Molecular delimitation of the seasonal killifishes of the Hypsolebias antenori species group (Cyprinodontiformes, Rivulidae), with description of two new species from the Caatinga coastal basins, northeastern Brazil

FIGURE 5. Hypsolebias bonita new species, MZUSP 129608, male, paratype, 38.6 mm SL: Brazil, Rio Grande do Norte, Baraúna, Furna Feia National Park.

FIGURE 4 in Molecular delimitation of the seasonal killifishes of the Hypsolebias antenori species group (Cyprinodontiformes, Rivulidae), with description of two new species from the Caatinga coastal basins, northeastern Brazil

FIGURE 4. Type-locality of Hypsolebias gongobira new species, Brazil, Ceará, Aquiraz, rio Pacoti basin.

FIGURE 15 in Molecular delimitation of the seasonal killifishes of the Hypsolebias antenori species group (Cyprinodontiformes, Rivulidae), with description of two new species from the Caatinga coastal basins, northeastern Brazil

FIGURE 15. Locality of H. antenori, Brazil, Ceará, Russas, seasonal pool in the floodplain of the rio Jaguaribe basin.

FIGURE 3. Hypsolebias gongobira new species, MZUSP 129607 in Molecular delimitation of the seasonal killifishes of the Hypsolebias antenori species group (Cyprinodontiformes, Rivulidae), with description of two new species from the Caatinga coastal basins, northeastern Brazil

FIGURE 3. Hypsolebias gongobira new species, MZUSP 129607, female, paratype, 35 mm SL: Brazil, Ceará, Aquiraz, rio Pacoti basin.

FIGURE 1 in Molecular delimitation of the seasonal killifishes of the Hypsolebias antenori species group (Cyprinodontiformes, Rivulidae), with description of two new species from the Caatinga coastal basins, northeastern Brazil

FIGURE 1. Map of northeastern Brazil showing the distribution of the Hypsolebias antenori species-group. Stars represent type localities, and circles indicate sampled localities. Hypsolebias bonita new species in pink, H. antenori in red, H. gongobira new species in orange (syntopic with H. longignatus), H. martinsi in yellow, H. coamazonicus in green, H. faouri in light blue, H. igneus in dark blue, and H. nudiorbitatus in purple. Dark blue lines represent hydrographic basins of the Caatinga ecoregions, Maranhão-Piauí (MAPE), Mid-Northeastern Caatinga (MNCE), Northeastern Atlantic Forest (NAFE) São Francisco (SFRE).