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Figure 1 in Phenotypic and molecular characterization of fluoroquinolone resistant Pseudomonas aeruginosa isolates in Palestine

Figure 1. Molecular phylogenetic analysis by Maximum Likelihood method based on the GyrA (A), ParC (B) and ParE (C) sequence from fluoroquinolone resistant P. aeruginosa isolated in Palestine. Reference sequences retrieved from Genbank for the GyrA (A), ParC (B) and ParE (C) genes were denoted by asterisks (*). Sequences from Palestine and reference sequences were used to construct the phylogenetic tree. Evolutionary analyses were conducted in MEGA6.

Figure 1 in Characterization of glutamine synthetase from the ammonium-excreting strain HM053 of Azospirillum brasilense

Figure 1. Transferase activity of glutamine synthetase. (A) Transferase activity of wild-type glutamine synthetase in the absence and presence of magnesium as well as with snake venom phosphodiesterase treatment; (B) Transferase activity of P347L glutamine synthetase in the absence and presence of magnesium, and with snake venom phosphodiesterase treatment. The activity of GS is expressed in µmol γ-glutamyl-hydroxamate.min-1.mg protein-1, given that the absorbance of 530 nm of 1 µmolγ-glutamylhydroxamate was 0.054. The total activity was determined in the absence of Mg2+ (-Mg2+) and the non-adenylylated (active) fraction was determined in the presence of 60 mM Mg2+ (+Mg2+). Samples were incubated at 30 ºC for 0, 10, 30 and 60 min before measuring activity. SVP-treated GS samples (+ SVP) were incubated with snake venom phosphodiesterase. GS activity reactions contained 3 µg of protein.

Figure 3 in Characterization of glutamine synthetase from the ammonium-excreting strain HM053 of Azospirillum brasilense

Figure 3. Prediction of the structure of glutamine synthetase from the mutant P347L. (A) Prediction of the P347L-GS structure. The amino acid marked in pink corresponds to leucine in strain HM053; (B) b1) Prediction structure of wild-type GS from amino acid 346 to 361. b2) Prediction structure of P347L-GS from amino acid 346 to 361. b3) Alignment of prediction structures of wildtype GS and P347L GS from amino acid 346 to 361. The amino acid marked in blue corresponds to the proline that is mutated in strain HM053. The amino acid marked in pink is leucine that replaced proline in the mutated amino acid in strain HM053.

Figure 2 in Characterization of glutamine synthetase from the ammonium-excreting strain HM053 of Azospirillum brasilense

Figure 2. Western blot assays of glutamine synthetase after treatment with snake venom phosphodiesterase. Samples (~ 0.3 µg GS protein) were separated by SDS‐PAGE followed by Western blotting with an anti‐GS antibody. A) Wild-type glutamine synthetase; B) P347L glutamine synthetase. Lane 1: GS after 0 min of incubation at 30 ºC without any treatment; lanes 2 to 5: GS after 0, 10, 30 and 60 min incubation at 30 ºC with snake venom phosphodiesterase. Lane 6: GS after 60 min incubation at 30 ºC without treatment.

opencc-by-4.0Jul 2024View details →

Data used in "The Backscatter Gating method for time, energy, and position resolution characterization of long form factor organic scintillators" by Hunter N. Ratliff et al.

This repository contains the raw experimental and PHITS-simulated data used in the JINST article “The Backscatter Gating method for time, energy, and position resolution characterization of long form factor organic scintillators” by Hunter N. Ratliff et al., available at <a href="https://doi.org/10.1088/1748-0221/19/07/P07002">https://doi.org/10.1088/1748-0221/19/07/P07002</a> (the accepted manuscript can also be found at <a href="https://hdl.handle.net/11250/3145287">https://hdl.handle.net/11250/3145287</a> and <a href="https://hratliff.com/publications/">https://hratliff.com/publications/</a>).   The data consists of three top-level directories, each with various subdirectories.  Within the “PHITS-simulated-data” directory are the PHITS simulations (including input and output files) used in producing Figures 4 and 5 in the manuscript, showing energy spectra in the bar for BSG events from a Cs-137 emission for placement of the source on the bar and on the BSG detector and with spacings between the bar and BSG detector of 20, 80, and 150 mm, along with the energy-dependent spatial distribution of these recoil electrons in the bar.   The other two top-level directories contain raw data produced by the CAEN CoMPASS software when acquiring data experimentally.  In the analysis for each measurement, the contents of the “RAW” folders were used.  The “Energy-calibrations-of-CeBr3” directory contains the energy spectra of the CeBr3 detector (used as the BSG detector) when exposed to a variety of radioactive sources, used together to energy-calibrate the CeBr3 detector.  The “Backscatter-gating-measurements” directory contains all of the list-mode data acquired with CoMPASS used for all of the other experimental measurements presented in the manuscript.  It contains subdirectories for varied sources, source positions along the bar, distances between the bar and BSG detector, measurements with the source affixed to the BSG detector instead, and a few miscellaneous measurements.  The “images” directories within each measurement’s directory (at the same level as the “RAW” folders) contain images produced by the analysis script written for this work used for diagnostics and presenting analyzed data.

Fig. 10. Males possess a in Characterization of stridulatory structures and sounds of the larger Mexican pine beetle, Dendroctonus approximatus (Coleoptera: Curculionidae: Scolytinae)

Fig. 10. Males possess a well-developed file on the lef elytron a), with a relatively underdeveloped file on the right elytron b). The development of a file in female D. approximatus was highly variable and ofen almost non-existent. Lef elytron of female with a well-developed file, relative to most other observed female files c), and of female with poorly-developed file d).

Fig. 7. Waveform a in Characterization of stridulatory structures and sounds of the larger Mexican pine beetle, Dendroctonus approximatus (Coleoptera: Curculionidae: Scolytinae)

Fig. 7. Waveform a) and spectrogram b) of simple chirps produced by a male (first and third chirps) and female (second and fourth chirps) when paired together in gallery. The spectral profile c) was taken at the center time of the first chirp, highlighted in a and b. Center time is the point during a sample about which energy is divided equally.

Fig. 8. Waveform a in Characterization of stridulatory structures and sounds of the larger Mexican pine beetle, Dendroctonus approximatus (Coleoptera: Curculionidae: Scolytinae)

Fig. 8. Waveform a) and spectrogram b) of overlapping chirps produced by female and male D. approximatus. The first chirp in the sequence was produced by the female and is repeated approximately every 0.5 s. The female chirp overlaps with male chirps between 2 and 5 s in the recording. Sound occurring just past 4 s was not produced by either beetle, but rather was accidental noise produced by the recorder.

Fig. 9 in Characterization of stridulatory structures and sounds of the larger Mexican pine beetle, Dendroctonus approximatus (Coleoptera: Curculionidae: Scolytinae)

Fig. 9. Abdominal segments of male a) and female b) D. approximatus. The male plectrum is shown circled in a).

Fig. 6. Waveform a in Characterization of stridulatory structures and sounds of the larger Mexican pine beetle, Dendroctonus approximatus (Coleoptera: Curculionidae: Scolytinae)

Fig. 6. Waveform a), and spectrogram b) of a male interrupted chirp showing individual syllables within each chirp.

Fig. 5. Waveform a in Characterization of stridulatory structures and sounds of the larger Mexican pine beetle, Dendroctonus approximatus (Coleoptera: Curculionidae: Scolytinae)

Fig. 5. Waveform a), and spectrogram b) of female simple chirp in a disturbance context. The waveform shows the relative amplitude in generic units and the spectrogram shows frequency over time with darker shades indicating higher relative energy.

Fig. 4. Waveform a in Characterization of stridulatory structures and sounds of the larger Mexican pine beetle, Dendroctonus approximatus (Coleoptera: Curculionidae: Scolytinae)

Fig. 4. Waveform a), spectrogram b), and spectral profile c) of male simple chirp in a disturbance context. The waveform shows the relative amplitude in generic units and the spectrogram shows frequency over time with darker shades indicating higher relative energy. The spectral profile was taken at the midpoint of the first chirp, highlighted in a and b.

Fig. 2 in Characterization of stridulatory structures and sounds of the larger Mexican pine beetle, Dendroctonus approximatus (Coleoptera: Curculionidae: Scolytinae)

Fig. 2. File length was measured posteriorly from the most posterior ridge (see lower arrow) up anteriorly to the last well-developed ridge (top arrow). Ridges at the anterior edge become poorly developed (circle) and these were not measured. See Methods for further description.

Fig. 3 in Characterization of stridulatory structures and sounds of the larger Mexican pine beetle, Dendroctonus approximatus (Coleoptera: Curculionidae: Scolytinae)

Fig. 3. Temporal and spectral characteristics assessed for simple chirps made in a disturbance context. Train of individual pulses a), waveform b), and spectrogram c) from sample chirp produced by male in a disturbance context. The peak frequency (3.468 kHz) is noted for the first recorded chirp (occurring at 0.3 s).

Fig. 1 in Characterization of stridulatory structures and sounds of the larger Mexican pine beetle, Dendroctonus approximatus (Coleoptera: Curculionidae: Scolytinae)

Fig. 1. Phloem slide positioned under dissecting scope. The ultrasonic electronic insertion microphone is shown positioned in the center of the slide (see circle), inserted into the tunnel entrance. A small piezo transducer, shown at the far end of the phloem slide, was not used in recordings for this study.

Fig. 1 in New species diversity revealed from molecular and morphological characterization of gall-inducing Calophya spp. (Hemiptera: Calophyidae) from Brazilian peppertree

Fig. 1. Adult male Calophya from Ubu, Carapina, and Salvador populations (A), adult male Calophya terebinthifolii (B), 5th instar from Ubu (C), Calophya latiforceps from Salvador and Carapina (D) and C. terebinthifolii (E).

opencc-by-4.0Mar 2015View details →

Fig. 4 in Mexican strains of Hirsutella isolated from Diaphorina citri (Hemiptera: Liviidae): Morphologic and molecular characterization

Fig. 4. Production of mucilaginous colonies by Hirsutella citriformis Mexican strains under dark conditions. Mucilaginous colony produced by strain INIFAP- Hir-1 (lef) and colonies without mucilage in strain IB-Hir-1 (right), both afer 5 days of culture under dark conditions.

opencc-by-4.0Mar 2015View details →

Fig. 1 in Mexican strains of Hirsutella isolated from Diaphorina citri (Hemiptera: Liviidae): Morphologic and molecular characterization

Fig. 1. Phylogenetic tree constructed with sequences of ITS1-5.8S-ITS2 of 19 Hirsutella species enlisted in the NCBI and 7 Mexican strains isolated from D. citri in Mexico. Percentages from bootstrap analysis that support branches in the tree are shown in the respective nodes. Scale represents the number of substitutions/100 nucleotides.

opencc-by-4.0Mar 2015View details →

Fig. 5 in Mexican strains of Hirsutella isolated from Diaphorina citri (Hemiptera: Liviidae): Morphologic and molecular characterization

Fig. 5. Mortality of Diaphorina citri caused by Hirsutella citriformis blastospores. Values followed by the same letter did not differ statistically (Tukey; P = 0.05). Lines in the bars indicate SD.