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1,233
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ShareScore release 0.9.0
Dataset results
1,233 results for “hepatocytes”
Exploiting microRNA and mRNA profiles generated in vitro from carcinogen-exposed primary mouse hepatocytes for predicting in vivo genotoxicity and carcinogenicity (miRNA)
GEO Series GSE72014. Homo sapiens; Rattus norvegicus; Mus musculus. 156 samples. Type: Non-coding RNA profiling by array.
Crnic Institute Human Trisome Project - Trisomy 21 Model Atlas: PolyA RNA-seq from human iPSC-derived Hepatocyte-like cells
GEO Series GSE296449. Homo sapiens. 20 samples. Type: Expression profiling by high throughput sequencing.
Comparative analysis of gene expression upon IFNα stimulation in hepatocyte-like cells [IFN]
GEO Series GSE288203. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
Expression data from HepG2 hepatocytes treated with sugarcane top ethanolic extract (STEE) and control HepG2 cells.
GEO Series GSE243412. Homo sapiens. 6 samples. Type: Expression profiling by array.
Liver transcriptomic profile of wild type and hepatocyte-specific ATF6 knockout mice feeding CD-HFD
GEO Series GSE285265. Mus musculus. 11 samples. Type: Expression profiling by high throughput sequencing.
Brap regulates liver morphology and hepatocyte turnover via modulation of the Hippo pathway
GEO Series GSE196012. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.
Hepatocyte identity and zonal position determine tumourigenic potential of mutant β-catenin
GEO Series GSE275864. Mus musculus. 48 samples. Type: Expression profiling by high throughput sequencing; Other.
Data from: Genome-scale characterization of toxicity-induced metabolic alterations in primary hepatocytes
Context-specific Genome-scale Metabolic Network Reconstructions (GENREs) provide a means to understand cellular metabolism at a deeper level of physiological detail. Here, we use transcriptomics data from chemically exposed rat hepatocytes to constrain a GENRE of rat hepatocyte metabolism and predict biomarkers of liver toxicity using the Transcriptionally Inferred Metabolic Biomarker Response (TIMBR) algorithm. We profiled alterations in cellular hepatocyte metabolism following in vitro exposure to four toxicants (acetaminophen, carbon tetrachloride, 2,3,7,8-tetrachlorodibenzodioxin, and trichloroethylene) for six hours. TIMBR predictions were compared with paired fresh and spent media metabolomics data from the same exposure conditions. Agreement between computational model predictions and experimental data led to the identification of specific metabolites and thus metabolic pathways associated with toxicant exposure. Here, we identified changes in the TCA metabolites citrate and alpha-ketoglutarate along with changes in carbohydrate metabolism and interruptions in ATP production and the TCA Cycle. Where predictions and experimental data disagreed, we identified testable hypotheses to reconcile differences between the model predictions and experimental data. The presented pipeline for using paired transcriptomics and metabolomics data provides a framework for interrogating multiple omics datasets to generate mechanistic insight of metabolic changes associated with toxicological responses.
Data from: Hepatocyte-specific deletion of TIPARP, a negative regulator of the aryl hydrocarbon receptor, is sufficient to increase sensitivity to dioxin-induced wasting syndrome
The aryl hydrocarbon receptor (AHR) mediates the toxic effects of dioxin (2,3,7,8-tetrachlorodibenzo-p-dioxin; TCDD), which include thymic atrophy, steatohepatitis, and a lethal wasting syndrome in laboratory rodents. Although the mechanisms of dioxin toxicity remain unknown, AHR signaling in hepatocytes is necessary for dioxin-induced liver toxicity. We previously reported that loss of TCDD-inducible poly(ADP-ribose) polymerase (TIPARP/PARP7/ARTD14), an AHR target gene and mono-ADP-ribosyltransferase, increases the sensitivity of mice to dioxin-induced toxicities. To test the hypothesis that TIPARP is a negative regulator of AHR signaling in hepatocytes, we generated Tiparpfl/fl mice in which exon 3 of Tiparp is flanked by loxP sites, followed by Cre-lox technology to create hepatocyte-specific (Tiparpfl/flCreAlb) and whole-body (Tiparpfl/flCreCMV; TiparpEx3-/-) Tiparp null mice. Tiparpfl/flCreAlb and TiparpEx3-/- mice given a single injection of 10 g/kg dioxin did not survive beyond day 7 and 9, respectively, while all Tiparp+/+ mice survived the 30-day treatment. Dioxin-exposed Tiparpfl/flCreAlb and TiparpEx3-/- mice had increased steatohepatitis and hepatotoxicity as indicated by greater staining of neutral lipids and serum alanine aminotransferase activity than similarly treated wild-type mice. Tiparpfl/flCreAlb and TiparpEx3-/- mice exhibited augmented AHR signalling, denoted by increased dioxin-induced gene expression. Metabolomic studies revealed alterations in lipid and amino acid metabolism in liver extracts from Tiparpfl/flCreAlb mice compared with wild-type mice. Taken together, these data illustrate that TIPARP is an important negative regulator of AHR activity, and that its specific loss in hepatocytes is sufficient to increase sensitivity to dioxin-induced steatohepatitis and lethality.
Data from: From the cover: three-dimensional (3d) heparg spheroid model with physiologically relevant xenobiotic metabolism competence and hepatocyte functionality for liver toxicity screening.
Effective prediction of human responses to chemical and drug exposure is of critical importance in environmental toxicology research and drug development. While significant progress has been made to address this challenge using invitro liver models, these approaches often fail due to inadequate tissue model functionality. Herein, we describe the development, optimization, and characterization of a novel three-dimensional (3D) spheroid model using differentiated HepaRG cells that achieve and maintain physiologically relevant levels of xenobiotic metabolism (CYP1A2, CYP2B6, and CYP3A4/5). This invitro model maintains a stable phenotype over multiple weeks in both 96- and 384-well formats, supports highly reproducible tissue-like architectures and models pharmacologically- and environmentally important hepatic receptor pathways (ie AhR, CAR, and PXR) analogous to primary human hepatocyte cultures. HepaRG spheroid cultures use 50–100× fewer cells than conventional two dimensional cultures, and enable the identification of metabolically activated toxicants. Spheroid size, time in culture and culture media composition were important factors affecting basal levels of xenobiotic metabolism and liver enzyme inducibility with activators of hepatic receptors AhR, CAR and PXR. Repeated exposure studies showed higher sensitivity than traditional 2D cultures in identifying compounds that cause liver injury and metabolism-dependent toxicity. This platform combines the well-documented impact of 3D culture configuration for improved tissue functionality and longevity with the requisite throughput and repeatability needed for year-over-year toxicology screening.
Safety and Efficacy Study of Mix Vaccine in Hepatocyte Carcinoma Patient
ClinicalTrials.gov study NCT02338778. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Safety Study of Adenovirus Hepatocyte Growth Factor to Treat Ischemic Heart Disease
ClinicalTrials.gov study NCT01925352. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Bone Marrow Stem Cells as a Source of Allogenic Hepatocyte Transplantation in Homozygous Familial Hypercholesterolemia
ClinicalTrials.gov study NCT00515307. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Analysis of the Role of Hepatocyte SLAMF3 Receptor and Drug Resistance Proteins (MDR) in Resistance to Treatment With Sorafenib in CHC Patients
ClinicalTrials.gov study NCT03113604. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Hepatocyte Matrix Implant Study
ClinicalTrials.gov study NCT00935454. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Hepatocyte Growth Factor (HGF) Concentration in Myocardial Infarction
ClinicalTrials.gov study NCT01233336. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Hepatocyte Transplantation in Liver Failure
ClinicalTrials.gov study NCT00805610. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Safety and Tolerability of Hepatocyte-like Cell Therapy for Liver Failure and Small-for-Size Syndrome
ClinicalTrials.gov study NCT07276568. IPD Sharing: NO. Countries: 0. Publications: 0.
Effect of Meal Timing in T2D on Hepatocytes SRIT1 and Clock Genes
ClinicalTrials.gov study NCT04405895. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Allogenic Hepatocyte Transplantation Into Periduodenal Lymph Nodes
ClinicalTrials.gov study NCT04496479. IPD Sharing: NO. Countries: 1. Publications: 0.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.