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972 results for “morphological barcode”
FIGURE 2. Rhododendron baihuaense. A, Flowering shoot. B, Scales. C, Flower. D, Mature fruit. E in A new species of Rhododendron (Ericaceae) from the Gaoligong Mountains, Yunnan, China, supported by morphological and DNA barcoding data
FIGURE 2. Rhododendron baihuaense. A, Flowering shoot. B, Scales. C, Flower. D, Mature fruit. E, Pistil and calyx. F, Stamen. G, Corolla, cut open.
FIGURE 1 in A new species of Rhododendron (Ericaceae) from the Gaoligong Mountains, Yunnan, China, supported by morphological and DNA barcoding data
FIGURE 1. Geographical distribution of Rhododendron baihuaense, R. hanceanum and R. genestierianum (detailed distribution information of these 3 species were obtained from Chinese Virtual Herbarium, http://www.cvh.org.cn).
Morphology and mini-barcodes: The inclusion of larval sampling and NGS-based barcoding improves robustness of ecological analyses of mosquito communities
<p class="Normal0">A significant proportion of vector-borne diseases are transmitted by blood-sucking dipterans, including mosquitoes. Understanding transmission risks requires accurate identification of species across heterogenous habitats, but many cryptic and polymorphic species are overlooked when using morphological identification. Estimates of mosquito diversity are typically based on adult female trapping methods which tend to target host-seeking species and may represent a biased snapshot of community structure. Unfortunately, diversity estimates based on larval data are rarely included in mosquito ecological analyses. We carried out adult and larval sampling over six months in Singapore using an integrative approach of morphological identification and molecular delineation with mini-barcodes (313 bp) generated on a Next Generation Sequencing platform to obtain species estimates. We collected 3201 mosquitoes across 58 species (14 genera). Notably, 16 species were collected only through larval sampling and 22 species were only resolved using mini-barcodes. Of the latter we identified three morphologically similar species groups and documented several intraspecific polymorphisms. We compared adult-only data against a full dataset (adult + larval + mini-barcode). The species accumulation curves reached an asymptote for all but one site when using the latter; non-metric multidimensional scaling (NMDS) revealed that mosquito communities were only well separated when using the full dataset. Overall, the latter reflects a more defined and accurate community structure across all sites. We find that several mosquito species were generally influenced by tree cover, rainfall and presence of large water bodies, further supporting the idea that many species are niche-specific. <i>Synthesis and applications</i>. We report the first successful use of mini-barcodes on mosquitoes and demonstrate its utility in delineating multiple challenging species groups. We recommend the use of both morphological and molecular identification methods for ecological studies and vector surveillance. Misidentification in species estimation, especially for medically relevant insect groups can lead to conflicting reports and slows down vector control efforts. We provide evidence that varying sampling techniques, particularly of the larval stages for holometabolous insects, is important in generating a robust dataset for downstream analyses. Together with DNA barcoding, this integrative approach helps to minimize error cascades when designing management strategies.</p>
Figure 1 in Two new species of Hudsonimyia Roback, 1979 (Diptera: Chironomidae: Tanypodinae) from Neotropical Region unveiled by morphology and DNA barcoding
Figure 1. Hudsonimyia caissara sp. nov.: adult male. (A) Head, frontal view. (B) Apex of antenna. (C) Thorax. (D) Wing. (E–G) Tibial spurs of P1 (E), P2 (F), and P3 (G). (H) Tergite IX and anal point. (I) Hypopygium with tergite IX and anal point removed, left: dorsal aspect, right: ventral aspect.
Figure 6 in Two new species of Hudsonimyia Roback, 1979 (Diptera: Chironomidae: Tanypodinae) from Neotropical Region unveiled by morphology and DNA barcoding
Figure 6. Hudsonimyia araxa sp. nov.: larva. (A) Head with chaetotaxy, left: dorsal aspect, right: ventral aspect. (B) Antenna. (C) Maxillary palp detail. (D) Mandible. (E) Mentum and M appendage. (F). Ligula and paraligula. (G) Pecten hypopharyngis. (H) Abdomen.
Figure 2 in Two new species of Hudsonimyia Roback, 1979 (Diptera: Chironomidae: Tanypodinae) from Neotropical Region unveiled by morphology and DNA barcoding
Figure 2. Hudsonimyia caissara sp. nov.: pupa. (A) Thoracic horn with basal lobe. (B) Thoracic horn surface opposite to plastron plate. (C) Abdominal segments with chaetotaxy, anal lobe and male genital sac, right: ventral aspect, left: dorsal aspect. (D) Shagreen pattern on abdominal segments surface.
Figure 5 in Two new species of Hudsonimyia Roback, 1979 (Diptera: Chironomidae: Tanypodinae) from Neotropical Region unveiled by morphology and DNA barcoding
Figure 5. Hudsonimyia araxa sp. nov.: pupa. (A) Thoracic horn with basal lobe. (B) Thoracic horn surface opposite to plastron plate. (C) Abdominal segments with chaetotaxy and anal lobe and male genital sac, right: ventral aspect, left: dorsal aspect. (D) Shagreen pattern on abdominal segments surface.
Figure 4 in Two new species of Hudsonimyia Roback, 1979 (Diptera: Chironomidae: Tanypodinae) from Neotropical Region unveiled by morphology and DNA barcoding
Figure 4. Hudsonimyia araxa sp. nov.: adult male and female. (A) Male head, frontal view. (B) Male thorax. (C) Male wing. (D) Hypopygium, left: ventral aspect, right: dorsal aspect. (E–G) Female tibial spurs of P1 (E), P2 (F), and P3 (G). (H) Female genitalia, ventral view.
Figure 3 in Two new species of Hudsonimyia Roback, 1979 (Diptera: Chironomidae: Tanypodinae) from Neotropical Region unveiled by morphology and DNA barcoding
Figure 3. Hudsonimyia caissara sp. nov.: larva. (A) Head with chaetotaxy, left: dorsal aspect, right: ventral aspect. (B) Antenna. (C) Maxillary palp detail. (D) Mandible. (E) Mentum and M appendage. (F). Ligula and paraligula. (G) Pecten hypopharyngis.
FIGURES 3–6 in Descriptions of two new species of Hemileucinae (Lepidoptera: Saturniidae) from the region of Muzo in Colombia-evidence from morphology and DNA barcodes
FIGURES 3–6. Wing patterns of Leucanella bonillensis n. sp. and closely related species. 3. Male Holotype, dorsal view; 4. Idem, ventral view; 5. Male of Leucanella acutissima, dorsal (left) and ventral (right) views; 6. Male of Leucanella newmani dorsal (left) and ventral (right) views.
FIGURES 7–15 in Descriptions of two new species of Hemileucinae (Lepidoptera: Saturniidae) from the region of Muzo in Colombia-evidence from morphology and DNA barcodes
FIGURES 7–15. Genitalia patterns of Leucanella bonillensis n. sp. and closely related species. 7. Holotype genitalia, dorsal view (genitalia prep. TD#171; 8. Idem, ventral view; 9. Idem, edeagus; 10. Genitalia of Leucanella acutissima, dorsal view (genitalia prep. TD#176); 11. Idem, ventral view; 12. Idem, edeagus; 13. Genitalia of Leucanella newmani, dorsal view (genitalia prep. TD#169); 14. Idem, ventral view; 15. Idem, edeagus.
FIGURE 1 in Descriptions of two new species of Hemileucinae (Lepidoptera: Saturniidae) from the region of Muzo in Colombia-evidence from morphology and DNA barcodes
FIGURE 1. Single most parsimonious tree (L=98, CI=0.84, RI=0.96) obtained from the phylogenetic analysis of the DNA barcode sequences for the specimens of the genus Leucanella. Each specimen is identified by its ProcessID code (see Table 1). Branch length is proportional to the number of substitutions, and values above branches are the number of inferred changes (FAST optimization) and bootstrap supports, respectively.
FIGURES 24–31 in Descriptions of two new species of Hemileucinae (Lepidoptera: Saturniidae) from the region of Muzo in Colombia-evidence from morphology and DNA barcodes
FIGURES 24–31. Genitalia patterns of Cerodirphia zulemae n. sp. and closely related species. 24. 7th tergite of the Holotype of C. zulemae, dorsal view (genitalia prep. TD#141); 25. Same structure, Cerodirphia apunctata (genitalia prep. S–RR#57); 26. Same structure, Cerodirphia brunnea (genitalia prep. TD#174); 27. Same structure, Cerodirphia speciosa (genitalia prep. RR#295); 28. 7th sternite of the Holotype of C. zulemae, ventral view; 29. Same structure, C. apunctata; 30. Same structure, C. brunnea; 31. Same structure, C. speciosa.
FIGURES 16–23 in Descriptions of two new species of Hemileucinae (Lepidoptera: Saturniidae) from the region of Muzo in Colombia-evidence from morphology and DNA barcodes
FIGURES 16–23. Wing patterns of Cerodirphia zulemae n. sp. and closely related species. 16. Male Holotype, dorsal vew; 17. Female Allotype, dorsal view; 18. Male paratype, dorsal view; 19. Idem, ventral view; 20. Male of Cerodirphia apunctata, dorsal view (French Guyana, crossroad RN2-Cacao, 25 i 1999, leg. R. Rougerie, ex larva–emerged 30 vii 1999, genitalia prep. S–RR#57); 21. Male of C. apunctata, dorsal view (Venezuela, Bolivar, road El Dorado – Sta Elena, km18, la Escalera, 1400m asl, 7–20 xi 1990, leg. P. Bleuzen, genitalia prep. C. Lemaire # 5718). 22. Male of Cerodirphia speciosa, dorsal view (French Guyana, Cayenne, i 1972, leg. J.J. de Granville, genitalia prep RR#295); 23. Male of Cerodirphia brunnea, dorsal view (Bolivia, La Paz, Nor Yungas, Carrasco, 1450m asl, xi 1990, leg. T. Decaëns & G. Lecourt).
FIGURES 32–51 in Descriptions of two new species of Hemileucinae (Lepidoptera: Saturniidae) from the region of Muzo in Colombia-evidence from morphology and DNA barcodes
FIGURES 32–51. Male genitalia patterns of Cerodirphia zulemae n. sp. and closely related species. 32. Genitalia of the Holotype of C. zulemae, dorsal view (genitalia prep. TD#141); 33. Idem, ventral view; 34. Idem, lateral view; 35. Idem, lateral view of the edeagus; 36. idem, dorsal view of the edeagus; 37. Genitalia of Cerodirphia apunctata, dorsal view (genitalia prep. S–RR#57); 38. Idem, ventral view; 39. Idem, lateral view; 40. Idem, lateral view of the edeagus; 41. idem, dorsal view of the edeagus; 42. Genitalia of Cerodirphia brunnea, dorsal view (genitalia prep. TD#174); 43. Idem, ventral view; 44. Idem, lateral view; 45. Idem, lateral view of the edeagus; 46. idem, dorsal view of the edeagus; 47. Genitalia of Cerodirphia speciosa, dorsal view (genitalia prep. RR#295); 48. Idem, ventral view; 49. Idem, lateral view; 50. Idem, lateral view of the edeagus; 51. idem, dorsal view of the edeagus.
FIGURES 52–54 in Descriptions of two new species of Hemileucinae (Lepidoptera: Saturniidae) from the region of Muzo in Colombia-evidence from morphology and DNA barcodes
FIGURES 52–54. Female genitalia patterns of Cerodirphia zulemae n. sp. 52. Genitalia of the Allotype, dorsal view (genitalia prep. TD#184); 53. Idem, ventral view; 54. Idem, lateral view.
FIGURE 2 in Descriptions of two new species of Hemileucinae (Lepidoptera: Saturniidae) from the region of Muzo in Colombia-evidence from morphology and DNA barcodes
FIGURE 2. Strict consensus of the 9 most parsimonious trees (L=244, CI=75, RI=89) obtained from the phylogenetic analysis of the DNA barcode sequences for the specimens of the genus Cerodirphia. Each specimen is identified by its ProcessID code (see Table 1). Branch length is proportional to the number of substitutions, and values above branches are the number of inferred changes (FAST optimization) and bootstrap supports, respectively.
FIGURE 6 in Are Bryodrilus parvus Nurminen, 1970 and Bryodrilus librus (Nielsen and Christensen, 1959) (Annelida: Enchytraeidae) really different species? A revision based on DNA barcodes and morphological data
FIGURE 6. Type materials of B. parvus. A–C: type 107432, 6D: type 107433. (In clove oil.). A. Segments IV–VI (phg = pharyngeal glands, oesophageal appendages in VI marked with white arrows, spermathecae marked with black arrows). B. Sperm funnels (marked with black arrows). C. Clitellar glands, dorsal view. D. Clitellar glands, ventral view (m = male copulatory organs). Lack of ventral glands marked with black arrow.
FIGURE 4. A in Are Bryodrilus parvus Nurminen, 1970 and Bryodrilus librus (Nielsen and Christensen, 1959) (Annelida: Enchytraeidae) really different species? A revision based on DNA barcodes and morphological data
FIGURE 4. A. Nephridium in 9/10, ectal duct originates medioventrally (marked with black arrow). Stained, on slide. Ellef Island, Canada. B. Nephridia in posterior segments, ectal duct originates posteroventrally (marked with black arrow, i = intestine). In vivo. Mezőföld, Hungary. C. Coelomocytes (marked with black arrow). In vivo. Kiskunság, Hungary. D. Coelomocytes (marked with black arrows). In vivo. Russebukta, Svalbard. E. Sperm funnel (marked with black arrow) Stained, on slide. Ellef Island, Canada. F. Sperm funnel (marked with black arrow) In vivo. Mezőföld, Hungary.
FIGURE 2. A in Are Bryodrilus parvus Nurminen, 1970 and Bryodrilus librus (Nielsen and Christensen, 1959) (Annelida: Enchytraeidae) really different species? A revision based on DNA barcodes and morphological data
FIGURE 2. A. Lateral and ventral chaetal bundles in IX–X. In vivo. Mezőföld, Hungary. B. Epidermal glands in transversal rows. In vivo. Kiskuság, Hungary. C. Clitellar glands, dorsal view. In vivo. Mezőföld, Hungary. D. Clitellar glands, dorsal view. Hyalin gland cells are dominant. Unstained, on slide. Fairbanks, Alaska. E. Ventral view of clitellar glands between the male copulatory organs. m= penial bulbs, black arrow shows the area without granular gland cells. Stained, on slide. Blåbukta, Svalbard. F. Clitellar glands, ventral view. Granular gland cells are present occasionally between the 2 male copulatory organs. Gland cells marked with black arrow, m= male copulatory organs. Stained, on slide. Zackenberg, Greenland.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.