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FIGURE 4 in Revision and phylogeny of the subaptera-group of Phyllodromica (Blattoptera: Blattellidae: Ectobiinae), including a parthenogenetic species and the evaluation of COI sequences for species identification (DNA barcoding)
FIGURE 4. Phyllodromica iberica morph #1, male, SEM pictures of tergal glandular structures. A–C, G Tergite 7. (A) Latero-frontal view from slightly above, anterior wall of trough slightly hollowed out anteriorly (white arrows); (B) lateral view of the glandular region from slightly above; (C) glandular region in dorsal view; (G) bristles of bristle field. D– F, H, I Tergite 8. (D) Dorsal view of the whole tergite, median emargination in the distribution of the bristles (white arrow heads); (E) latero-frontal view from slightly above; (F) dorsal view of the conelike process; (H) porous surface of the central mound; (I) soft villi on the surface of the conelike process. Abbreviations: bf bristle field, cm central mound, cp conelike process, m mound, r ridge, se sinusoidal edge, sp shallow pit, tt transversal trough. Identification: (A–C, E– I) Sp 270a/M4, (D) Sp 85/11.
FIGURE 3 in Revision and phylogeny of the subaptera-group of Phyllodromica (Blattoptera: Blattellidae: Ectobiinae), including a parthenogenetic species and the evaluation of COI sequences for species identification (DNA barcoding)
FIGURE 3. Phyllodromica iberica morph #1, male (holotype). A Thoracal nota. B–F Abdominal tergites 5–9. (B) Tergite 5 and (C) tergite 6 with membranous glands in the lateral region of the anterior margin of tergites (black arrows); (D) tergite 7, white arrow heads point to the shallow pouches appearing as crescent–shaped black shadows; (E) tergite 8; (F) tergite 9 on glass rod. G Terminalia with tergite 10 (sa), cerci and paraprocts. H Hook of left phallomere with the posterior end on the top. I Subgenital plate with remaining genital sclerites (without hook). Abbreviations: ap anterior process, bf bristle field, c cercus, cm central mound, cp conelike process, ll lateral lobe, m mound, mes mesonotum, met metanotum, ml median lobe, mp medio-anterior process, pml posterior median lobe, pro pronotum, r ridge between bristle fields, rp right paraproct, sa supraanal plate, se sinusoidal edge, sp shallow pits, tm tegmen, tr transversal ridge, tt transversal trough. Same scale for (B, C, G) and (D, E, H). Identification: Sp 292b/M3 (holotype).
FIGURE 2 in Revision and phylogeny of the subaptera-group of Phyllodromica (Blattoptera: Blattellidae: Ectobiinae), including a parthenogenetic species and the evaluation of COI sequences for species identification (DNA barcoding)
FIGURE 2. Phyllodromica subaptera (female). A Thoracal nota. B Abdominal tergite 5. C Subgenital plate with two apodemal processes (a). D Dorsal complex of genitalia with additional sclerite (as): ventral view, posterior end on top. E Additional sclerite in higher enlargement (equivalent to "black frame" in D). F Ventral complex of genitalia: laterosternal shelf with intersternal folds between the arms. Abbreviations: as additional sclerite, a apodemal process, bd dorsal sclerite of basivalvula, bv ventral sclerite of basivalvula, c cercus, i intersternal fold, is intercalary sclerite, l laterosternal shelf, ls laterosternite IX, pl posterior lobe of valvifer II, pp paraproct, pt paratergites, T9 tergite 9, T10 tergite 10, v valves. Same scale for (B, C) and (D, F). Identification: Sp 203c/W4.
FIGURE 1. A–C P in Revision and phylogeny of the subaptera-group of Phyllodromica (Blattoptera: Blattellidae: Ectobiinae), including a parthenogenetic species and the evaluation of COI sequences for species identification (DNA barcoding)
FIGURE 1. A–C P. iberica morph #1, habitus. (A) Male in dorsal view; (B) female in dorsal and (C) ventral view. D Oothek of P. quadracantha (lateral view). Abbreviations: a antenna, c cercus, fl coxa of foreleg, h head, hl coxa of hindleg, mes mesonotum, met metanotum, ml coxa of midleg, pm palpus maxillaris, pro pronotum, sub subgenital plate, T2–T10 tergites 2–10, tm tegmen. Same scale in A and B. Localities: (A–C) Sp 449a, (D) Sp 500.
FIGURE 3 in Species delimitation in the Drosophila aldrichi subcluster (Diptera: Drosophilidae) using DNA sequences
FIGURE 3. Distribution map of the samples used in the molecular analysis. Specimens collected in Australia are not shown.
FIGURE 1 in Species delimitation in the Drosophila aldrichi subcluster (Diptera: Drosophilidae) using DNA sequences
FIGURE 1. Strict consensus tree of 54 most parsimonious trees in the combined analysis of all four genes – length = 693; CI = 0.693; RI = 0.718; RC = 0.497. Decay index is shown above the nodes. Numbers below the nodes are Bootstrap / Jackknife support values higher than 50 %. Each node is identified by a letter to relate with Fig. 2. The smaller cladogram highlights branching and support values for the ingroup.
FIGURE 2 in Species delimitation in the Drosophila aldrichi subcluster (Diptera: Drosophilidae) using DNA sequences
FIGURE 2. (A) Strict consensus tree of 700 most parsimonious trees in the combined mtDNA analysis – length = 479; CI = 0.683; RI = 0.753; RC = 0.514. Decay index is shown above the nodes. Bootstrap / Jackknife support values are presented below the node. Each node is identified by a letter to relate with Fig. 1. The smaller cladogram highlights branching and support values in for the ingroup. (B) Strict consensus tree of 13288 most parsimonious trees in the combined nuclear analysis – length = 194; CI = 0.789; RI = 0.606; RC = 0.563. Decay index is shown above the nodes. Bootstrap / Jackknife support values are presented below the node. Each node is identified by a letter to relate with Fig. 1.
Data for morphometric analysis and DNA barcode sequence for the new fish species Polymixia hollisterae
<p>Two datasets are provided to support the journal article (https://doi.org/10.1643/i2020112) by T. C. Grande and M. V. H. Wilson naming the new Bermuda fish species <em>Polymixia hollisterae</em>. The first dataset is for 2-D multivariate morphometric comparisons of selected specimens and species of the fish genus <em>Polymixia</em>. The file is in TPS format, as a plain text file, for use in the application MorphoJ. The data are for 27 specimens with pixel coordinates for 34 landmarks digitized in ImageJ and used to generate Fig. 13 in the referenced publication. The second dataset, published here courtesy of Dr. R. Eytan, is a mitochondrial DNA barcode sequence in fasta format for the second paratype specimen, a small juvenile of the new species. The specimen is only the third one known of the new species and the only one not from Bermuda. It was collected in the north-central Gulf of Mexico and is now deposited in the Harvard University MCZ fish collection as catalog number MCZ 174218. The fasta file can be used as input to the barcode identification function at boldsystems.org, although its original identification in BOLD was incorrect.</p>
Comparative Analysis of Complete Chloroplast Genomes and Multiple DNA Sequences Reveals Interspecific Relationships of C. bretschneideri and Related Species in China
<p><strong> ITS, and <em>LEAFY</em> intron 1 sequencing of 36 Crataegus accessions.</strong></p>
FIGURE 4 in Phylogenetic relationships of Discyphus scopulariae (Orchidaceae, Cranichideae) inferred from plastid and nuclear DNA sequences: evidence supporting recognition of a new subtribe, Discyphinae
FIGURE 4. Phylogenetic relationships in Spiranthinae inferred from nuclear (ITS) and plastid (rbcL, matK-trnK, trnL-trnF) DNA sequences by maximum likelihood (ML). The main tree is the ML tree; numbers under branches are bootstrap proportions from the ML bootstrap analysis. The inset on the upper left hand is the ML tree with branches drawn proportional to branch lengths. The major clades referred to in the text are marked as follows: a, Stenoptera clade; b, Prescottia clade; c, "core" Cranichidinae; d, Spiranthinae (excluding Discyphus). The position of Discyphus is indicated by an asterisk (*).
FIGURE 3 in Phylogenetic relationships of Discyphus scopulariae (Orchidaceae, Cranichideae) inferred from plastid and nuclear DNA sequences: evidence supporting recognition of a new subtribe, Discyphinae
FIGURE 3. Phylogenetic relationships in Spiranthinae inferred from nuclear (ITS) and plastid (rbcL, matK-trnK, trnL-trnF) DNA sequences by maximum parsimony (MP). The main tree is the strict consensus of 24 most parsimonious trees (MPTs) recovered by the analysis; numbers under branches are bootstrap proportions (from the MP bootstrap analysis). The inset on the upper left hand is one of the 24 MPTs with branches drawn proportional to branch length. The major clades referred to in the text are marked as follows: a, Stenoptera clade; b, Prescottia clade; c, "core" Cranichidinae; d, Spiranthinae (excluding Discyphus). The position of Discyphus is indicated by an asterisk (*).
FIGURE 2 in Phylogenetic relationships of Discyphus scopulariae (Orchidaceae, Cranichideae) inferred from plastid and nuclear DNA sequences: evidence supporting recognition of a new subtribe, Discyphinae
FIGURE 2. Discyphus scopulariae (from Coelho de Moraes 2171). A. Habit. B. Flower. C. Flower opened out between dorsal sepal and one lateral sepal. D. Dorsal sepal. E. Lateral sepal. F. Petal. G. Labellum. H. Column, ventral view. I. Column apex, side view. Single bar = 1 mm, double bar = 1 cm. Drawn by Judi Stone and originally published in Pridgeon et al. 2003: Fig. 181.1 (reproduced with permission).
FIGURE 1. Discyphus scopulariae. A in Phylogenetic relationships of Discyphus scopulariae (Orchidaceae, Cranichideae) inferred from plastid and nuclear DNA sequences: evidence supporting recognition of a new subtribe, Discyphinae
FIGURE 1. Discyphus scopulariae. A. Flowering plant in situ (Bahia, Brazil, Popovkin 338A). B−E. Another flowering plant removed from soil (Bahia, Brazil, Popovkin 900). C. Inflorescence. D. Roots and leaf from below. E. Close-up of the column apex from below with the pollinarium removed, showing the bifid rostellum remnant and the two stigmatic areas with pollinium fragments presumably deposited by an unrecorded pollinator. Photographers: Alex Popovkin (A−D), Isys Souza (E).
Analysis of RNA-seq, DNA target enrichment, and Sanger nucleotide sequence data resolves deep splits in the phylogeny of cuckoo wasps (Hymenoptera: Chrysididae)
<p>The wasp family Chrysididae (cuckoo wasps, gold wasps) comprises exclusively parasitoid and kleptoparasitic species, many of which feature a stunning iridescent coloration and phenotypic adaptations to their parasitic life style. Previous attempts to infer phylogenetic relationships among the family's major lineages (subfamilies, tribes, genera) based on Sanger sequence data were insufficient to statistically resolve the monophyly and the phylogenetic position of the subfamily Amiseginae and the phylogenetic relationships among the tribes Allocoeliini, Chrysidini, Elampini, and Parnopini (Chrysidinae). Here, we present a phylogeny inferred from nucleotide sequence data of 492 nuclear single-copy genes (230,915 aligned amino acid sites) from 94 species of Chrysidoidea (representing Bethylidae, Chrysididae, Dryinidae, Plumariidae) and 45 outgroup species by combining RNA-seq and DNA target enrichment data. We find support for Amiseginae being more closely related to Cleptinae than to Chrysidinae. Furthermore, we find strong support for Allocoeliini being the sister lineage of all remaining Chrysidinae, while Elampini represent the sister lineage of Chrysidini and Parnopini. Our study corroborates results from a recent phylogenomic investigation which revealed Chrysidoidea as likely paraphyletic</p>
PLATE 1 in Morphology and DNA sequences confirm the first Neotropical record for the Holarctic sepsid species Themira leachi (Meigen) (Diptera: Sepsidae)
PLATE 1. Morphology of Themira leachi from Cuba (photographed A–F; drawn M–R) and Europe (photographed G– L). Habitus: A, G; fore femoral modifications (anterior view): B, H; fore femoral modifications (posterior view): C, I; fore tibial modifications (anterior view): D, J; abdomen (lateral view, sternite bristles removed): E, K, N; abdomen (ventral view, sternite bristles removed): F, L, M; fore-femur (anterior view): O; fore-tibia (anterior view): P; hypopygium (dorsal view, setulation omitted): Q; 4th sternite (dorsal view): R. Scale bars for A, G: 1mm; B–D and H–J: 0.1mm; E, F, K, L: 0.5mm
FIGURE 2. Bayesian tree inferred from LSU gene DNA sequences. Posterior probabilities exceeding 50 in A new species of the genus Tripylina Brzeski, 1963 (Nematoda: Enoplida: Trischistomatidae) from Shanxi province, China
FIGURE 2. Bayesian tree inferred from LSU gene DNA sequences. Posterior probabilities exceeding 50% are given on appropriate clades. Nematode species and GenBank numbers are listed for each taxon.
FIGURE 1. Bayesian tree inferred from SSU gene DNA sequences. Posterior probabilities exceeding 50 in A new species of the genus Tripylina Brzeski, 1963 (Nematoda: Enoplida: Trischistomatidae) from Shanxi province, China
FIGURE 1. Bayesian tree inferred from SSU gene DNA sequences. Posterior probabilities exceeding 50% are given on appropriate clades. Nematode species and GenBank numbers are listed for each taxon.
FIGURE 2 in Insight into the validity of Leptobrachium guangxiense (Anura: Megophryidae): evidence from mitochondrial DNA sequences and morphological characters
FIGURE 2 Maximum parsimony (MP) tree, Maximum likelihood (ML) tree and Bayesian posterior probability (BPP) tree reconstructed from 1914 bp of 12S rRNA, tRNAval and 16S rRNA mitochondrial genes with Oreolalax rhodostigmatus and Leptolalax heteropus as outgroups. Numbers above branches represent bootstrap supports for MP/ML/BPP. Symbol (*) indicates nodes with well bootstrap supports for ML and MP (>90%) inferences and Bayesian posterior probabilities (BPP = 95%), and symbol (-) represents that node values are less than 50%. Sample numbers are included in Table 1.
FIGURE 1 in Insight into the validity of Leptobrachium guangxiense (Anura: Megophryidae): evidence from mitochondrial DNA sequences and morphological characters
FIGURE 1. Map of Southeast Asia showing samples of ingroup species used for mtDNA analysis. Sample numbers are included in Table 1.
FIGURE 3 in Insight into the validity of Leptobrachium guangxiense (Anura: Megophryidae): evidence from mitochondrial DNA sequences and morphological characters
FIGURE 3 Morphological characteristics of Leptobrachium guangxiense (voucher No.: NHMG200807002). A. Dorsolateral aspect, B. Iris color, C. Oral disc (voucher No.: NHMG_T200903001), D. Ventral aspect.
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.