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1,344 results for “: phylogenomics”
Delimiting the cryptic diversity and host preferences of Sycophila parasitoid wasps associated with oak galls using phylogenomic data
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A novel probe set for the phylogenomics and evolution of RTA spiders
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Phylogenomics-based click-beetle classification tackles multiple origins of phenotypic modifications
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Phylogenomics and deep convergence in cockroach hind-wing morphology
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A phylogenomic backbone for gastropod molluscs
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Ultraconserved element data for phylogenomic analysis of Ghatippus paschima jumping spider (Salticidae, Plexippini, Plexippina)
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Data from: The origin of the legumes is a complex paleopolyploid phylogenomic tangle closely associated with the Cretaceous-Paleogene (K-Pg) mass extinction event
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Data from: Populating a continent: Phylogenomics reveal the timing of Australian frog diversification
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Transcriptome assemblies associated with: A cnidarian phylogenomic tree fitted with hundreds of 18S leaves
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Phylogenomic analysis and morphological data suggest left-right swimming behavior evolved prior to the origin of the pelagic Phylliroidae (Gastropoda: Nudibranchia)
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A phylogenomic approach to resolving interrelationships of polyclad flatworms, with implications for life history evolution
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Datasets for Draco lineatus phylogenomics study
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Extensive introgression among strongylocentrotid sea urchins revealed by phylogenomics
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Diverge and conquer: Phylogenomics of southern Wallacean forest skinks (Genus: Sphenomorphus) and their colonization of the Lesser Sunda Archipelago
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MitoFinder: efficient automated large-scale extraction of mitogenomic data in target enrichment phylogenomics
<p><strong>MitoFinder: efficient automated large-scale extraction of mitogenomic data in target enrichment phylogenomics</strong></p> <p>Rémi Allio<sup>1</sup>, Alex Schomaker-Bastos<sup>2,†</sup>, Jonathan Romiguier<sup>1</sup>, Francisco Prosdocimi<sup>2</sup>, Benoit Nabholz<sup>1</sup>, and Frédéric Delsuc<sup>1</sup></p> <p><sup>1</sup><em>Institut des Sciences de l’Evolution de Montpellier (ISEM), CNRS, EPHE, IRD, Université de Montpellier, Montpellier, France.</em></p> <p><sup>2</sup><em>Laboratório Multidisciplinar para Análise de Dados (LAMPADA), Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil.</em></p> <p><sup>†</sup><em> In Memoriam (08/01/2015) </em></p> <p> </p> <p><em><strong>Correspondence</strong></em></p> <p>Rémi Allio</p> <p>Email: <a href="mailto:remi.allio@umontpelier.fr">remi.allio@umontpellier.fr</a></p> <p>Frédéric Delsuc</p> <p>Email: <a href="mailto:frederic.delsuc@umontpellier.fr">frederic.delsuc@umontpellier.fr</a></p> <p> </p> <p><strong><em>Running head</em></strong></p> <p>Mitochondrial signal from UCE capture data</p> <p> </p> <p><strong>Abstract</strong><strong> </strong></p> <p>Thanks to the development of high-throughput sequencing technologies, target enrichment sequencing of nuclear ultraconserved DNA elements (UCEs) now allows routinely inferring phylogenetic relationships from thousands of genomic markers. Recently, it has been shown that mitochondrial DNA (mtDNA) is frequently sequenced alongside the targeted loci in such capture experiments. Despite its broad evolutionary interest, mtDNA is rarely assembled and used in conjunction with nuclear markers in capture-based studies. Here, we developed MitoFinder, a user-friendly bioinformatic pipeline, to efficiently assemble and annotate mitogenomic data from hundreds of UCE libraries. As a case study, we used ants (Formicidae) for which 501 UCE libraries have been sequenced whereas only 29 mitogenomes are available. We compared the efficiency of four different assemblers (IDBA-UD, MEGAHIT, MetaSPAdes, and Trinity) for assembling both UCE and mtDNA loci. Using MitoFinder, we show that metagenomic assemblers, in particular MetaSPAdes, are well suited to assemble both UCEs and mtDNA. Mitogenomic signal was successfully extracted from all 501 UCE libraries allowing confirming species identification using COI barcoding. Moreover, our automated procedure retrieved 296 cases in which the mitochondrial genome was assembled in a single contig, thus increasing the number of available ant mitogenomes by an order of magnitude. By leveraging the power of metagenomic assemblers, MitoFinder provides an efficient tool to extract complementary mitogenomic data from UCE libraries, allowing testing for potential mito-nuclear discordance. Our approach is potentially applicable to other sequence capture methods, transcriptomic data, and whole genome shotgun sequencing in diverse taxa.</p> <p> </p> <p><strong><em>Figures & Tables</em></strong></p> <p><strong>Figure 1.</strong> Conceptualization of the pipeline used to assemble and extract UCE and mitochondrial signal from ultraconserved element sequencing data.</p> <p><strong>Figure 2</strong>. Comparison of the efficiency of the assemblers in terms of: A) computational time, B) number of potentially mitochondrial contigs identified, and C) number of mitochondrial genes annotated. Violin plots reflect the data distribution with a horizontal line indicating the median. Note that for the three metagenomic assemblers, 5 CPUs were used compared to 35 CPUs for Trinity. Plots were obtained using PlotsOfData (Postma & Goedhart 2019).</p> <p><strong>Figure 3.</strong> Phylogenomic relationships of ants (Formicidae). AA) Mito-nuclear phylogenetic differences among subfamily relationships based on the UCE and mtDNA supermatrices obtained with the assembler MetaSPAdes assembler. Clades corresponding to subfamilies were collapsed. Inter-subfamily relationships with UFBS < 95% were collapsed. Non-maximal node support values are reported. B) The topology obtained reflects the results of phylogenetic analyses based on the amino acid mitochondrial supermatrix (using MetaSPAdes as assembler). Histograms reflect the percent of UCEs (light grey) and mitochondrial genes (dark grey) recovered for each species. Illustrative pictures (*): <em>Diacamma sp</em>. (Ponerinae; top left), <em>Formica sp</em>. (Formicinae; top right), and <em>Messor barbarus </em>(Myrmicinae; bottom right).</p> <p><strong>Table 1. </strong>Summary statistics on assembly results according to the assembler used. The values are averages over the 501 assemblies, except for the assembly time, which is a median value. The two tables report specific statistics for A) ultraconserved elements data, and B) mitochondrial data. Note that 35 CPUs were used for Trinity whereas 5 CPUs were used for other assemblers.</p> <p><strong>Table 2.</strong> Statistical comparison between the performances of the different assemblers. Statistical significance was estimated with a paired non parametric test (paired wilcoxon test). *** = <em>p</em><0.001; ** = <em>p</em><0.01; * = <em>p</em><0.05; NS = <em>p</em>>0.05; and (+)/(-) is the result of the comparison between the row and the column.</p> <p> </p> <p><strong><em>Appendices</em></strong></p> <p><strong>Appendix S1.</strong> List of the 501 UCE libraries (SRA accessions) and associated metadata.</p> <p><strong>Appendix S2.</strong> Summary statistics on mitochondrial signal recovered per species and depending on the assembler used. The table provides the number of contigs and genes recovered with MitoFinder and the size of each annotated gene.</p> <p><strong>Appendix S3.</strong> Summary statistics of barcoding analyses. Detailed results for both BOLDsystem and Megablast analyses are provided for each CO1 recovered with MitoFinder using MetaSPAdes.</p> <p><strong>Appendix S4.</strong> Detailed results of tree distance analyses realized with Dquad (Ranwez, Criscuolo, & Douzery 2010). Trees obtained with each assembler with mitochondrial amino acid supermatrix, mitochondrial nucleotide supermatrix, and UCE nucleotide supermatrix were compared with each others.</p> <p><strong>Appendix S5</strong>. List of Genbank accession numbers for newly generated mitchondrial contigs.</p> <p> </p> <p><strong><em>Zenodo supplementary files</em></strong></p> <p><strong>Assembly_results.tar.gz</strong> Contains all contigs obtained for each species with the different assemblers implemented in MitoFinder.</p> <p><strong>MitoFinder_annotations.tar.gz</strong> Contains MitoFinder annotations for each species. (based on the contigs obtained with MetaSPAdes)</p> <p><strong>UCE_results.tar.gz</strong> Contains all annotated UCE obtained for each species after UCE identification with PHYLUCE. (MetaSPAdes)</p> <p><strong>Final_mtDNA_alignments.tar.gz</strong> Contains the final mitochondrial gene alignments. (MetaSPAdes)</p> <p><strong>Final_UCE_alignments.tar.gz</strong> Contains the final UCE alignments. (MetaSPAdes)</p> <p><strong>Final_mtDNA_matrices.tar.gz</strong> Contains the final mi tochondrial supermatrices (AA and NT) used for the phylogenetic analyses. (MetaSPAdes)</p> <p><strong>Metaspades_final_UCE_matrix.phy</strong> The final UCE supermatrix used for the phylogenetic analyses. (MetaSPAdes)</p>
Data from: Phylogenomics, biogeography and morphometrics reveal rapid phenotypic evolution in pythons after crossing Wallace's line
<p>Ecological opportunities can be provided to organisms that cross stringent biogeographic barriers towards environments with new ecological niches. Wallace's and Lyddeker's lines are arguably the most famous biogeographic barriers, separating the Asian and Australo-Papuan biotas. One of the most ecomorphologically diverse groups of reptiles, the pythons, is distributed across these lines, and are remarkably more diverse in phenotype and ecology east of Wallace's line in Australo-Papua. We used an anchored hybrid enrichment approach, with near complete taxon sampling, to extract mitochondrial genomes and 376 nuclear loci to resolve and date their phylogenetic history. Biogeographic reconstruction demonstrates that they originated in Asia around 38-45 Ma and then invaded Australo-Papua around 23 Ma. Australo-Papuan pythons display a sizeable expansion in morphological space, with shifts towards numerous new adaptive optima in head and body shape, coupled with the evolution of new micro-habitat preferences. We provide an updated taxonomy of pythons and our study also demonstrates how ecological opportunity following colonization of novel environments can promotemorphological diversification in a formerly ecomorphologically conservative group.</p>
Phylogenomics of monitor lizards and the role of competition in dictating body size disparity
<p>Organismal interactions drive the accumulation of diversity by influencing species ranges, morphology, and behavior. Interactions vary from agonistic to cooperative and should result in predictable patterns in trait and range evolution. However, despite a conceptual understanding of these processes, they have been difficult to model, particularly on macroevolutionary timescales and across broad geographic spaces. Here we investigate the influence of biotic interactions on trait evolution and community assembly in monitor lizards (<em>Varanus</em>). Monitors are an iconic radiation with a cosmopolitan distribution and the greatest size disparity of any living terrestrial vertebrate genus. Between the colossal Komodo dragon <em>Varanus komodoensis</em> and the smallest Australian dwarf goannas, <em>Varanus</em> length and mass vary by multiple orders of magnitude. To test the hypothesis that size variation in this genus was driven by character displacement, we extended existing phylogenetic comparative methods which consider lineage interactions to account for dynamic biogeographic history and apply these methods to Australian monitors and marsupial predators. Incorporating both exon-capture molecular and morphological datasets we use a combined evidence approach to estimate the relationships among living and extinct varaniform lizards. Our results suggest that communities of Australian <em>Varanus</em> show high functional diversity as a result of continent-wide interspecific competition among monitors but not with faunivorous marsupials. We demonstrate that patterns of trait evolution resulting from character displacement on continental scales are recoverable from comparative data and highlight that these macroevolutionary patterns may develop in parallel across widely distributed sympatric groups.</p>
Data from: Phylogenomics in Cactaceae: a case study using the chollas sensu lato (Cylindropuntieae, Opuntioideae) reveals a common pattern out of the Chihuahuan/Sonoran Deserts
PREMISE OF THE STUDY: Although numerous phylogenetic studies have been conducted in Cactaceae, whole plastome datasets have not been employed. We used the chollas to develop a plastome dataset for phylogeny reconstruction to test species relationships, biogeography, clade age and morphological evolution. METHODS: We developed a plastome dataset for most known diploid members of the chollas, 42 taxa, as well as for other members of Cylindropuntieae. Paired-end, raw reads from genome skimming were referenced-mapped onto a de novo plastome assembly of one species of cholla, Cylindropuntia bigelovii, which were used to build our plastome dataset that was analyzed using various methods. KEY RESULTS: Our plastome dataset resolved the phylogeny of the chollas, including most inter- and intraspecific relationships. Tribe Cylindropuntieae arose during the early Miocene in southern South America, ca. 18 mya, and is supported as sister to the South American clade Tephrocacteae. The (Micropuntia (Cylindropuntia + Grusonia)) clade most likely originated in the Chihuahuan Desert region around 16 mya, and then migrated into other North American desert regions. Key morphological characters for recognizing traditional taxonomic series (e.g., spiny fruit) in Cylindropuntia are mostly homoplasious. CONCLUSIONS: This study provides the first comprehensive plastome phylogeny for any clade within Cactaceae. Although widespread throughout western North American deserts, the most recent common ancestor of the chollas s.l. likely arose in the Chihuahuan Desert region during the mid-Miocene, with much of their species diversity arising in the early to mid-Pliocene, showing a strikingly similar pattern to other western North American Desert groups.
Data from: Phylogenomic approaches reveal how climate shapes patterns of genetic diversity in an African rain forest tree species
<p>The world's second largest expanse of tropical rain forest is in Central Africa and it harbours enormous species diversity. Population genetic studies have consistently revealed significant structure across central African rain forest plants, in particular a North-South genetic discontinuity around the equatorial line, in a continuous expanse of rain forest but where a climatic inversion is documented. Here, we took a phylogeographic approach by sequencing 351 nuclear markers in 112 individuals across the distribution of the African rain forest tree species Annickia affinis (Annonaceae). We showed for the first time that the North-South divide is the result of a single, major colonisation event across the climatic inversion from an ancestral population located in Gabon. We suggested that differences in ecological niche of populations located on either side of this inversion may have contributed to this phylogenetic discontinuity. We found evidence for inland dispersal, predominantly in northern areas, and variable demographic histories among genetic clusters, indicating that populations responded differently to past climate change. We show how newly-developed genomic tools can provide invaluable insights into our understanding of tropical rain forest evolutionary dynamics.</p>
Data from: Congruence and conflict in the higher-level phylogenetics of squamate reptiles: an expanded phylogenomic perspective
<p>Genome-scale data have the potential to clarify phylogenetic relationships across the tree of life, but have also revealed extensive gene tree conflict. This seeming paradox, whereby larger datasets both increase statistical confidence and uncover significant discordance, suggests that understanding sources of conflict is important for accurate reconstruction of evolutionary history. We explore this paradox in squamate reptiles, the vertebrate clade comprising lizards, snakes, and amphisbaenians. We collected an average of 5103 loci for 91 species of squamates that span higher-level diversity within the clade, which we augmented with publicly available sequences for an additional 17 taxa. Using a locus-by-locus approach, we evaluated support for alternative topologies at 17 contentious nodes in the phylogeny. We identified shared properties of conflicting loci, finding that rate and compositional heterogeneity drives discordance between gene trees and species tree and that conflicting loci rarely overlap across contentious nodes. Finally, by comparing our tests of nodal conflict to previous phylogenomic studies, we confidently resolve nine of the 17 problematic nodes. We suggest this locus-by-locus and node-by-node approach can be used to build consensus on which topological resolutions remain uncertain in phylogenomic studies of other contentious groups.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.