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4,004 results for “In vivo”
Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus
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In vivo transcriptome of Lactobacillus acidophilus and colonization impact on murine host intestinal gene expression
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In vivo x-ray diffraction and simultaneous EMG reveal the timecourse of myofilament lattice dilation and filament stretch
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Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors
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Data from: Phage resistance evolution in vitro is not reflective of in vivo outcome in a plant-bacteria-phage system
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Source data for: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro biofilm and in vivo implant infections
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Comparative molecular analysis of cancer behavior cultured in vitro, in vivo, and ex vivo
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Ultrafast two-photon fluorescence imaging of cerebral blood circulation in the mouse brain in vivo
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CT DICOM studies from: In vivo measurements of lung volumes in ringed seals: insights from biomedical imaging
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In Vivo Spectral Distortions of Infrared Luminescent Nanothermometers Compromise Their Reliability
<p>Dataset accompanying figures presented in: <a href="https://zenodo.org/record/3906054#.XvNS5ygzbtQ">https://zenodo.org/record/3906054#.XvNS5ygzbtQ</a></p>
Ultrafast photochemistry produces superbright short-wave infrared dots for low-dose in vivo imaging
<p>Dataset corresponding to the manuscript available at <a href="https://zenodo.org/record/3906552#.XvNokCgzbtQ">https://zenodo.org/record/3906552#.XvNokCgzbtQ</a></p>
Adult_Hooded_Rat_In-vivo tethered
Drawing uploaded to scidraw.io on: 16 November 2019
Figure 2 from: Logoyda L (2020) Efficient validated method of HPLC to determine amlodipine in combinated dosage form containing amlodipine, enalapril and bisoprolol and in vitro dissolution studies with in vitro/ in vivo correlation. Pharmacia 67(2): 55-61. https://doi.org/10.3897/pharmacia.67.e48220
Figure 2 Representative chromatogram of amlodipine in combinated tablets (1- peak of bisoprolol, 2- peak of enalapril, 3 – peak of amlodipine).
Ag2S nanoheaters with multiparameter sensing for reliable thermal feedback during in vivo tumor therapy
<p>The emergence of luminescence nanothermometry in bio and nanomedicine has enabled achievements outside the reach of conventional techniques. For instance, it has provided real-time monitoring to in vivo thermal therapies of tumors, a mandatory requirement for these techniques to work safely and efficiently. However, the reliability of intratumoral thermal readings is currently in question due to the presence of artefacts caused by the inhomogeneous optical properties of biological tissues. This work demonstrates how it is possible to avoid, under specific conditions, these artefacts and reach precise and reliable in vivo intratumoral thermal feedback during in vivo photothermal treatments. The method proposed is based on the use of luminescent nanoparticles capable of multiparametric thermal sensing. The results demonstrate how the convergence of the different thermal readouts becomes a solid indicator of their reliability. It is shown how this new approach makes possible precise (thermal uncertainties below 1 °C) intratumoral thermal feed-back, while simple, efficient, and minimally invasive in vivo thermal treatments of surface tumors is carried out. Results included in this work provide an ingenious route toward the consolidation of luminescence nanothermometry as a convincing technique for high sensitivity preclinical thermal sensing, while also constituting a step toward improved photothermal therapies.</p>
Transcriptomic data and analyses of shMeg3 muscle in vitro and in vivo
<p>Formation of skeletal muscle is among the most striking examples of cellular plasticity in animal tissue development, and while muscle progenitor cells are reprogrammed by epithelial-mesenchymal transition (EMT) to migrate during embryonic development, regulation of EMT in postnatal myogenesis remains poorly understood. Here, we demonstrate that the long noncoding RNA (lncRNA) <em>Meg3</em> regulates EMT in myoblast differentiation and skeletal muscle regeneration. Chronic inhibition of <em>Meg3</em> in C2C12 myoblasts induced EMT, and supressed cell state transitions required for differentiation. Furtheremore, adenoviral <em>Meg3</em> knockdown compromised muscle regeneration, which was accompanied by abnormal mesenchymal gene expression and interstitial cell proliferation. Transcriptomic and pathway analyses of <em>Meg3-</em>depleted C2C12 myoblasts and injured skeletal muscle revealed a significant dysregulation of EMT-related genes, and identified TGFβ as a key upstream regulator. Importantly, inhibition of TGFβR1 and its downstream effectors, and the EMT-related transcriptional repressor Snai2, restored many aspects of myogenic differentiation in <em>Meg3</em>-depleted myoblasts <em>in vitro</em>. We further demonstrate that reduction of <em>Meg3-</em>dependent Ezh2 activity results in epigenetic alterations associated with TGFβ activation. Thus, <em>Meg3</em> regulates myoblast identity to facilitate progression into muscle differentiation.</p>
Data from: An in vivo three-dimensional Magnetic Resonance Imaging-based averaged brain collection of the neonatal piglet (Sus scrofa)
Due to the fact that morphology and perinatal growth of the piglet brain is similar to humans, use of the piglet as a translational animal model for neurodevelopmental studies is increasing. Magnetic resonance imaging (MRI) can be a powerful tool to study neurodevelopment in piglets, but many of the MRI resources have been produced for adult humans. Here, we present an average in vivo MRI-based atlas specific for the 4-week-old piglet. In addition, we have developed probabilistic tissue classification maps. These tools can be used with brain mapping software packages (e.g. SPM and FSL) to aid in voxel-based morphometry and image analysis techniques. The atlas enables efficient study of neurodevelopment in a highly tractable translational animal with brain growth and development similar to humans.
Data from: All-optical recording and stimulation of retinal neurons in vivo in retinal degeneration mice
Here we demonstrate the application of a method that could accelerate the development of novel therapies by allowing direct and repeatable visualization of cellular function in the living eye, to study loss of vision in animal models of retinal disease, as well as evaluate the time course of retinal function following therapeutic intervention. We use high-resolution adaptive optics scanning light ophthalmoscopy to image fluorescence from the calcium sensor GCaMP6s. In mice with photoreceptor degeneration (rd10), we measured restored visual responses in ganglion cell layer neurons expressing the red-shifted channelrhodopsin ChrimsonR over a six-week period following significant loss of visual responses. Combining a fluorescent calcium sensor, a channelrhodopsin, and adaptive optics enables all-optical stimulation and recording of retinal neurons in the living eye. Because the retina is an accessible portal to the central nervous system, our method also provides a novel non-invasive method of dissecting neuronal processing in the brain.
Data from: Beauty is more than skin deep: a non-invasive protocol for in vivo anatomical study using micro-CT
Microcomputed tomography (μCT) is a widely used tool in biomedical research, employed to investigate tissues and bone structures of small mammals in vivo. The application of in vivo μCT scanning in non-medical studies greatly lags behind the rapid advancements made in the biomedical field wherein the methodology has evolved to allow for longitudinal studies and eliminate the need to sacrifice the animal. Ecological and evolutionary studies often involve morphological measurements of a large sample of live animals; however, the potential of in vivo μCT imaging as a method for data acquisition has yet to be delineated. Here, we describe a protocol for in vivo μCT imaging of the internal anatomy of reptiles and amphibians, commonly used study organisms in ecological and evolutionary research. We consider the skeletal and extraskeletal (i.e. osteoderms) bones of a lizard as a case study to elucidate the potential of in vivo μCT imaging. First, we explore the effects of various parameter settings on radiation dose, scan time and image quality. Secondly, we develop a protocol to immobilize and restrain study organisms during scanning without need for the administration of anaesthetics and compare the results of the in vivo protocol to images obtained post-mortem. To immobilize animals, we replace the use of anaesthetics by cooling, thereby allowing the use of previously unsuitable rotating gantry μCT scanners that are readily available in scientific institutions. The resultant image quality of in vivo μCT scans is similar to that of post-mortem μCT scans, especially in the abdominal region. We discuss the effect of tube voltage, distance to X-ray source and metal filtration on radiation dose, and how these parameters could be altered to reduce the cumulative radiation dose while maintaining optimal image quality. The proposed in vivo μCT protocol offers a new approach to acquire anatomical information for non-biomedical studies. We offer specific suggestions as to how the protocol can be employed to suit a variety of model organisms.
Data from: A nutrient mediates intraspecific competition between rodent malaria parasites in vivo
Hosts are often infected with multiple strains of a single parasite species. Within-host competition between parasite strains can be intense and has implications for the evolution of traits that impact patient health, such as drug resistance and virulence. Yet the mechanistic basis of within-host competition is poorly understood. Here, we demonstrate that a parasite nutrient, para-aminobenzoic acid (pABA), mediates competition between a drug resistant and drug susceptible strain of the malaria parasite, Plasmodium chabaudi. We further show that increasing pABA supply to hosts infected with the resistant strain worsens disease and changes the relationship between parasite burden and pathology. Our experiments demonstrate that, even when there is profound top-down regulation (immunity), bottom-up regulation of pathogen populations can occur and that its importance may vary during an infection. The identification of resources that can be experimentally controlled opens up the opportunity to manipulate competitive interactions between parasites and hence their evolution.
Data from: Increasing procaspase 8 expression using repurposed drugs to induce HIV infected cell death in ex vivo patient cells
HIV persists because a reservoir of latently infected CD4 T cells do not express viral proteins and are indistinguishable from uninfected cells. One approach to HIV cure suggests that reactivating HIV will activate cytotoxic pathways; yet when tested in vivo, reactivating cells do not die sufficiently to reduce cell-associated HIV DNA levels. We recently showed that following reactivation from latency, HIV infected cells generate the HIV specific cytotoxic protein Casp8p41 which is produced by HIV protease cleaving procaspase 8. However, cell death is prevented, possibly due to low procaspase 8 expression. Here, we tested whether increasing procaspase 8 levels in CD4 T cells will produce more Casp8p41 following HIV reactivation, causing more reactivated cells to die. Screening 1277 FDA approved drugs identified 168 that increased procaspase 8 expression by at least 1.7-fold. Of these 30 were tested for anti-HIV effects in an acute HIVIIIb infection model, and 9 drugs at physiologic relevant levels significantly reduced cell-associated HIV DNA. Primary CD4 T cells from ART suppressed HIV patients were treated with one of these 9 drugs and reactivated with αCD3/αCD28. Four drugs significantly increased Casp8p41 levels following HIV reactivation, and decreased total cell associated HIV DNA levels (flurbiprofen: p = 0.014; doxycycline: p = 0.044; indomethacin: p = 0.025; bezafibrate: P = 0.018) without effecting the viability of uninfected cells. Thus procaspase 8 levels can be increased pharmacologically and, in the context of HIV reactivation, increase Casp8p41 causing death of reactivating cells and decreased HIV DNA levels. Future studies will be required to define the clinical utility of this or similar approaches.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.