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MARS EXPRESS MARS PFS EDR SECOND EXTENSION MISSION DATA V1.0

The Mars Express PFS data set contains raw (CODMAC Level 2) measurements from the Planetary Fourier Spectrometer collected during the first extension Mars orbit phases.

GIOTTO EXTENDED MISSION, MAGNETOMETER V1.0

This dataset contains results from the magnetometer flown aboard the Giotto spacecraft, and recorded during its flyby of Comet P/Grigg-Skjellerup in July of 1992. It contains both raw magnetometer readings from periods during 3 July to 11 July, as well as files containing time-averages of the magnetic field vectors during the encounter period.

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 3 3352 V1.0

This is a Mars Express Radio Science data set, collected during the extended mission phase 2010-01-01 to 2012-12-31. It is a Global Gravity measurement and covers the time 2012-07-17T11:25:38.000 to 2012-07-17T13:06:57.500.

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 3 3293 V1.0

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 3 3899 V1.0

Transcriptional profiling of thymus tissue from rats flown on the SLS-2 mission

The Spacelab Life Sciences (SLS) program was a series of payloads that used the facilities of an entire Spacelab module to conduct a variety of life science investigations. The general objective of the research in the SLS programs was to study the acute and chronic changes that living systems undergo during exposure to the space environment. The specific objective of the Spacelab Life Sciences-2 (SLS-2) mission was to study the structural and functional changes occurring in the bone, muscle, blood, and balance systems of rats in response to space flight. Rats were housed in individual cages in the Research Animal Holding Facility (RAHF) and natural calcium in the food bar was replaced with a non-radioactive calcium isotope (40Ca). The General Purpose Transfer Unit (GPTU) was used to transfer rat cages from the RAHFs to the General Purpose Work Station (GPWS), enabling the crew to perform inflight experiment procedures on the rodents. To this end, a flight group (FLT) of 38-day old male Sprague-Dawley rats were launched from Kennedy Space Center (KSC) on 10/18/1993 and housed in Research Animal Housing Facility (RAHF) on the shuttle for 14 days before being returned alive to Earth. After splashdown, the FLT were decapitated, and dissection occurred on 11/1/1993. Asynchronous ground control animals (GC) were exposed identical environmental conditions as the FLT group except for noise profile, acceleration and vibration. GC animals were housed in SpaceLab-3 (SL-3) simulated cages and dissection occurred on 11/1/1993. Upon dissection, thymus tissues were preserved in liquid nitrogen and stored at -70 C before RNA was extracted. This dataset features 6 FLT and 6 GC biological replicates. In preparation for RNA extraction, thymus tissues were prepared by generating wedge cuts from the top and bottom of the tissue, designated as Cut1 and Cut2. RNA was then extracted from both sets of cuts for all samples. For all FLT and GC samples, purified RNA from Cut2 was selected for library construction. Additionally, purified RNA from Cut1 was also selected for library construction for only one FLT and one GC sample, FLT_F15_Cut1 and GC_G12_Cut1, respectively, which also included four technical replicates (LibRep1-4). All libraries were generated using the QIAseq UPX 3’ Transcriptome kit that enables 3’ gene expression analysis from purified RNA and sequenced around 40 M clusters at SE 93 bp.

Transcriptional profiling of tibialis anterior muscle from mice flown on the RR-23 mission

The objective of the Rodent Research-23 mission (RR-23) was to better understand the effects of spaceflight on the eyes, specifically on the structure and function of the arteries, veins, and lymphatic vessels that are needed to maintain vision. To this end, twenty male, C57BL/6J, 16-17 weeks-old mice were delivered to the International Space Station (ISS) on SpaceX-21 in a single transporter, transferred to two rodent habitats, and maintained in microgravity for 38 days. Flight mice were then returned to Earth alive (January 13th, 2021). After splashdown in the Atlantic Ocean, mice were transported to Kennedy Space Center via helicopter. The 20 Flight, 20 Habitat Ground Control (HGC), and 20 Vivarium Ground Control (VGC) mice were removed from Rodent Transporters (Flight and HGC) or vivarium cages (VGC), placed into shipping containers, and flown to Texas A and M University. There, mice underwent post-flight procedures, before euthanasia and tissue collection. Flight, HGC and VGC animals were euthanized and dissected on Jan 14th, 17th or 20th of 2021, respectively. Right tibialis anterior muscle samples were preserved by immersion in liquid nitrogen and stored at -80C until RNA was extracted, and libraries generated and sequenced (target 60 M clusters per sample, PE 150 bp). This dataset features 9 samples from the Flight group, 9 samples from the HGC group, and 9 samples from the VGC group.

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 3 3295 V1.0

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 3 3357 V1.0

Multi-modal transcriptional and chromatin accessibility analysis of brains from mice flown on the RR-3 mission

The Rodent Research-3 (RR-3) mission was sponsored by the pharmaceutical company Eli Lilly and Co. and the Center for the Advancement of Science in Space to study the effectiveness of a potential countermeasure for the loss of muscle and bone mass that occurs during spaceflight. Twenty BALB/c, 12-weeks old female mice (ten controls and ten treated) were flown to the ISS and housed in the Rodent Habitat for 39-42 days. Twenty mice of similar age, and matching sex and strain were used for ground controls housed in identical hardware and matching ISS environmental conditions. Basal controls were housed in standard vivarium cages. Spaceflight, ground controls and basal groups had blood collected, then were euthanized, had one hind limb removed, and finally whole carcasses were stored at -80 C until dissection. All mice in this data set received only the control/sham injection. Brain samples from three flight and three ground control animal groups were cut in half between hemispheres. One hemisphere of each brain was used for generating spatially resolved transcriptional profiling data. Hemispheres were cryosectioned so that 2 consecutive sections from the hippocampus of each brain was placed on Visium Gene Expression arrays. Samples were fixed, stained with Hematoxylin and Eosin and imaged. Imaging was followed by tissue permeabilization to release mRNA molecules from cells for capture onto the array surface. Subsequently, following the 10XGenomics Visium Gene Expression protocol, Spatial Transcriptomics RNA-seq libraries were prepared and sequenced. The other hemisphere of each brain was used for single nuclei RNA-seq and ATAC-seq using the 10X Multiome protocol. In addition, bulk RNA-seq (ribodepleted, target depth of 60 M clusters, PE 150 bp) was performed from a pool of RNA extracted from 10-20 sections from each of 3 flight and 3 ground control samples.

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 3 2978 V1.0

This is a Mars Express Radio Science data set, collected during the extended mission phase 2010-01-01 to 2012-12-31. It is a Global Gravity measurement and covers the time 2011-06-29T23:21:45.000 to 2011-06-30T02:00:20.500.

Metagenomic analysis of feces from mice flown on the RR-9 mission

The objective of the Rodent Research-9 (RR-9) mission was to use mice to understand the molecular basis of phenomena that affect astronauts during long-duration spaceflight, particularly visual impairment and joint tissue degradation. To this end, a flight group (FLT) of 10-week-old male C57BL/6J mice was launched from Kennedy Space Center (KSC) on 8/14/2017 and housed in Rodent Habitats on the ISS for 33 days before being returned alive to Earth. After splashdown in the Pacific Ocean, the animals were transported to Loma Linda University (LLU) for testing, euthanasia and dissection on 9/18/2018. A Basal Control (BSL) was housed in standard cages at Kennedy Space Center (KSC) and euthanized one day after launch of the FLT animals (8/15/2017). Ground Control (GC) and Vivarium Control (VIV) studies were planned to commence at KSC approximately one-week after the conclusion of the flight experiments. However, all the GC and VIV mouse studies at KSC had to be cancelled due to Hurricane Irma and potential adverse effects on the animal housing facility. The GC and VIV studies were therefore rescheduled and begun in May, 2018. The GC was euthanized and dissected 6/18/2018 - 6/20/2018, while the VIV was euthanized and dissected 6/22/2018 - 6/23/2018. Because this resulted in a different cohort of mice being used for the GC and VIV controls as compared to the flight (FLT) and basal (BSL) groups, two cohort controls were included in the study. The first, Cohort Control 1 (CC_C1), was from the same cohort as the FLT and BSL animals, and was sacrificed and dissected 4 days after the FLT group (9/22/2017). The second, Cohort Control 2 (CC_C2), was from the same cohort as the GC and VIV animals, and was sacrificed and dissected 2-8 days after the GC and VIV groups, (6/24/2018 - 6/26/2018). The CC_C1 and CC_C2 groups were housed in standard cages and fed standard chow in contrast to all other groups which received Rodent Foodbars. To clarify the connections between treatment groups and animal cohorts, the following group abbreviations are used in the sample metadata: Flight (FLT_C1); Basal (BSL_C1); Ground Control (GC_C2); Vivarium Control (VIV_C2), Cohort Control 1 (CC_C1); Cohort Control 2 (CC_C2). Fecal pellets were isolated directly from mice during dissection and preserved by flash freezing in liquid nitrogen before stored at -80 C. DNA was then extracted, shotgun metagenomic libraries generated, and libraries sequenced (target 10 M clusters at PE 250 bp). Metagenomic data was generated from the following groups: Basal Control (n=5), Ground Control (n=5), Vivarium Control (n=5), Cohort Control 1 (n=5), Cohort Control 2 (n=5), Flight (n=5).

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 5 3819 V1.0

This is a Mars Express Radio Science data set, collected during the extended mission phase 2015-01-01 to 2016-12-31. It is a Occultation measurement and covers the time 2015-09-13T01:07:47.000 to 2015-09-13T01:31:41.949.

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 5 3808 V1.0

restrictednotspecifiedMar 2025View details →

Precipitation Measurement Missions Data Access

Tropical Rainfall Measuring Mission (TRMM) data products are currently available from 1998 to the present. Global Precipitation Measurement (GPM) mission data products are currently available from March 2014 to the present. TRMM and GPM are joint missions between NASA and the Japan Aerospace Exploration Agency (JAXA) designed to monitor and study global precipitation. Here we outline the types of products currently available for TRMM and GPM and the various ways to query, view and download these precipitation products.

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 5 3692 V1.0

This is a Mars Express Radio Science data set, collected during the extended mission phase 2015-01-01 to 2016-12-31. It is a Occultation measurement and covers the time 2015-02-06T23:26:07 to 2015-02-08T21:49:50.500.

Transcriptional analysis of thymus from mice flown on the RR-6 Mission

The objective of the Rodent Research-6 (RR-6) study was to evaluate muscle atrophy in mice during spaceflight and to test the efficacy of a novel therapeutic to mitigate muscle wasting. The experiment involved an implantable subcutaneous nanochannel delivery system (nDS; between scapula), which delivered the drug formoterol (FMT; a selective Beta-2 adrenoceptor agonist), over the course of time. To this end, a cohort of forty 32-weeks-old female C57BL/6NTac mice were either sham operated or implanted with vehicle or treatment-filled nDS, and launched in two Transporters (20 mice per Transporter) on SpaceX-13 on December 15, 2017. They were transferred to Rodent Habitats onboard the International Space Station (ISS), and maintained in microgravity for 29 days (N=20, Live Animal Return [LAR]), or >50 days (N=20, ISS Terminal). After 29 days, the 20 LAR animals were returned live to back to Earth on January 13, 2018. After splashdown, the animals were ambulatory on-ground for ~4 days, until all subjects were processed during one day of dissections. There were two Baseline groups of animals sacrificed (LAR Baseline & FLT Baseline; N=20; 40 animals; ~36 weeks old) at Kennedy Space Center (KSC; 12/9/17). A Ground Control group mimicked the Flight LAR group, which was housed at KSC, then shipped alive, to Novartis' facilities, where both the LAR and LAR Ground Control groups were processed (~41 weeks old; 1/16/18). All were anesthetized with isoflurane, blood samples were obtained by closed-chest cardiac puncture, and the animals were euthanized by exsanguination and thoracotomy. The 20 ISS Terminal mice were anesthetized via intraperitoneal injection of ketamine/xylazine/acepromazine over the course of a four days of dissections (2/6/18 until 2/9/18; 53-56 days after launch; 44 weeks old at time of on-orbit dissections). Blood samples and euthanasia were conducted the same as LAR and Baseline. Following blood draw and hind limb dissection, the ISS-terminal animal carcasses were wrapped in aluminum foil, placed in a ziploc bag and placed in storage at -80C or colder until return. The ISS-terminal Ground Controls (at KSC) followed the same euthanasia timeline, methods, and preservation. The final processing of frozen ISS-terminal, frozen ISS-terminal Ground Controls and frozen 0-day FLT baseline animals were completed at Houston Methodist Research Institute, in Houston, TX (5/21/18 until 5/24/18). GeneLab received samples of thymus from only sham treated animals (no drug treated animals) from the following groups Flight: LAR (n=9), ISS Terminal (n=10); Ground Controls: LAR GC (N=9), ISS Terminal GC (N=10), LAR Baseline (n=10) ISS Terminal Baseline (n=9). Total RNA was extracted and sequenced at a target depth of 60 M clusters per sample (ribodepleted, paired end 150).

Transcriptional analysis of femoral skin from mice flown on the RR-5 mission

The objective of the Rodent Research-5 (RR-5) study was to evaluate bone loss in mice during spaceflight and to determine if treatment with a modified version of NEL-like molecule-1 (NELL-1) can reduce or prevent bone loss that would otherwise occur during spaceflight. To this end, a cohort of forty 30-weeks-old female BALB/cAnNTac mice were flown to the ISS and housed in the Rodent Habitat. Six days after launch half of the mice were treated with NELL-1 (10 mg/kg in 0.3 ml PBS), while the other half were treated with vehicle control (0.3 ml PBS). Fourteen days after launch animals were again treated with NELL-1 or vehicle control as before, except that all animals were also injected with the bone marker, calcein green (20 mg/kg in 0.1 ml). Injections of vehicle, NELL-1, and bone markers were intraperitoneal. After all forty mice on orbit received two treatments; ten control mice and ten experimental mice were randomly selected for live animal return (LAR). At approximately 30 days after launch the twenty LAR mice were transported live back to Earth. Animals were allowed to recover for 30 days in standard habitats before euthanasia via intraperitoneal injection with ketamine/xylazine. During the recovery, the animals received another two treatments. GeneLab received RNA later preserved femoral skin from nine live animal return and ten matching ground control mice. These were from the vehicle control animals only. RNA was extracted, libraries generated (stranded, ribodepleted) and sequenced (target 60 M clusters at PE 150 bp).

Bulk RNA sequencing and spatially resolved transcriptional profiling of hippocampi from mice flown on the RR-10 mission

The objective of the Rodent Research-10 mission (RR-10) was to investigate how spaceflight affects the cellular and molecular mechanisms of normal bone tissue regeneration in space. To this end, ten (10) 14-15 weeks-old female B6129SF2/J Wild Type (WT), and ten (10) 14-15 weeks-old female B6;129S2-Cdkn1atm1Tyj/J (p21-null) mice received a pre-flight subcutaneous injection of the bone marker (Alizarin Red), and were then delivered to the ISS aboard SpaceX-21. At 7 days before euthanasia, all 20 mice received an intraperitoneal (IP) injection with a bone formation marker (Calcein). At 48 +/- 2 hours before euthanasia, all 20 mice received an IP injection with a second dose of Calcein as well as a cell proliferation marker (BrdU). Then, following 28-29 days in microgravity, the Flight mice were euthanized. Following removal of hindlimbs, carcasses were wrapped in aluminum foil, preserved in the CryoChiller, and stored at -80 C or colder until return to Earth. In addition to the Flight group, three ground control groups were also part of the study: Basal (representing the pre-launch state), Vivarium (standard vivarium housing for the same duration of time as flight), and Ground (flight habitat in the International Space Station Environment Simulator, ISSES). Twenty mice (10 of each strain) were included in each of these control groups (except Vivarium which included 12 of each strain). These were treated, euthanized and processed on the same schedule and in the same manner as the flight samples. This study includes bulk RNA sequencing and spatially resolved transcriptional profiling data from hippocampi from 5 WT flight animals and 5 WT ground control animals. Hippocampi from the right hemisphere were embedded and cryosectioned. Cryosections were either processed for bulk RNA sequencing or placed on gene expression arrays, stained and imaged. Imaging was followed by tissue permeabilization to release mRNA molecules from cells for capture onto the array surface. Subsequently, spatial transcriptomics libraries were prepared and sequenced.

MARS EXPRESS MARS MRS 1/2/3 EXTENDED MISSION 5 3715 V1.0

This is a Mars Express Radio Science data set, collected during the extended mission phase 2015-01-01 to 2016-12-31. It is a Occultation measurement and covers the time 2015-03-28T14:51:00 to 2015-03-29T04:34:29.999.