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opennotspecifiedMar 2018View details →

FIGURE 2 in Aspergillus fuscicans (Aspergillaceae, Eurotiales), a new species in section Usti from Argentinean semi-arid soil

FIGURE 2. Strict consensus phylogenetic cladogram constructed with maximum parsimony analysis with BenA sequences. MP and NJ bootstrap values>50% are shown above and below branches, respectively. Terminal nodes given as GenBank accession number and species name.

opennotspecifiedMar 2018View details →

FIGURE 1. Aspergillus fuscicans. Colonies 7 d, 25 in Aspergillus fuscicans (Aspergillaceae, Eurotiales), a new species in section Usti from Argentinean semi-arid soil

FIGURE 1. Aspergillus fuscicans. Colonies 7 d, 25 ºC. A. CYA. B. Reverse. C. MEA. C´. Colonies 30 d, 25 ºC. D–F. Conidiophores and conidia. G. Conidia. H–I. Hülle cells. Bars D–E, H–I = 20 μm, F–G = 10 μm.

FIGURE 5. Aspergillus allahabadii grown for 7 in Phylogeny of Aspergillus section Terrei with two new records from the China General Microbiological Culture Collection Centre

FIGURE 5. Aspergillus allahabadii grown for 7 days at 25°C on six media: A) MEA, B) CYA, C) YES, D) OA, E) DG, and F) CREA. G–L) Conidiophores and conidia, Scale bars = 10 μm.

FIGURE 4. Aspergillus alabamensis grown for 7 in Phylogeny of Aspergillus section Terrei with two new records from the China General Microbiological Culture Collection Centre

FIGURE 4. Aspergillus alabamensis grown for 7 days at 25°C on six media: A) MEA, B) CYA, C) YES, D) OA, E) DG, and F) CREA. G–L) Conidiophores and conidia, Scale bars = 10 μm.

FIGURE 2 in Phylogeny of Aspergillus section Terrei with two new records from the China General Microbiological Culture Collection Centre

FIGURE 2. Phylogenetic tree inferred from the partial CaM sequences showing the relationships among members of Aspergillus section Terrei. ML bootstrap support values over 75% and Bayesian posterior predictive values over 0.95 are shown above the branches. The tree is rooted with Aspergillus flavipes (NRRL 302T).

FIGURE 1 in Phylogeny of Aspergillus section Terrei with two new records from the China General Microbiological Culture Collection Centre

FIGURE 1. Phylogenetic tree inferred from the partial ITS sequences showing the relationships among members of Aspergillus section Terrei. ML bootstrap support values over 75% and Bayesian posterior predictive values over 0.95 are shown above the branches. The tree is rooted with Aspergillus flavipes (NRRL 302T).

FIGURE 3 in Phylogeny of Aspergillus section Terrei with two new records from the China General Microbiological Culture Collection Centre

FIGURE 3. Phylogenetic tree inferred from the partial BenA sequences showing the relationships among members of Aspergillus section Terrei. ML bootstrap support values over 75% and Bayesian posterior predictive values over 0.95 are shown above the branches. Strongly supported branches have bootstrap support values of 100, Bayesian posterior predictive values of 1.00 are shown in bold. The tree is rooted with Aspergillus flavipes (NRRL 302T).

opencc-zeroSep 2021View details →

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Chemical analyses of three lysergic acid amide-producing Aspergillus species and sequences for phylogenetic analyses of associated enzymes

Ergot alkaloids derived from lysergic acid have impacted humanity as contaminants of crops and as the bases of pharmaceuticals prescribed to treat dementia, migraines, and other disorders. Several plant-associated fungi in the Clavicipitaceae produce lysergic acid derivatives, but many of these fungi are difficult to culture and manipulate. Some Aspergillus species, which may be more ideal experimental and industrial organisms, contain an alternate branch of the ergot alkaloid pathway but none were known to produce lysergic acid derivatives. We mined genomes of Aspergillus species for ergot alkaloid synthesis (eas) gene clusters and discovered three species––A. leporis, A. homomorphus, and A. hancockii––had eas clusters indicative of the capacity to produce a lysergic acid amide. In culture, A. leporis, A. homomorphus, and A. hancockii produced lysergic acid amides, predominantly lysergic acid α-hydroxyethylamide (LAH). Aspergillus leporis and A. homomorphus produced high concentrations of LAH and secreted most of their ergot alkaloid yield into the culture medium. Phylogenetic analyses indicated genes encoding enzymes leading to the synthesis of lysergic acid were orthologous to those of the lysergic acid amide-producing Clavicipitaceae; however, genes to incorporate lysergic acid into an amide derivative evolved from different ancestral genes in the Aspergillus species. Our data demonstrate fungi outside the Clavicipitaceae produce lysergic acid amides and indicate the capacity to produce lysergic acid evolved once, but the ability to insert it into LAH evolved independently in Aspergillus species and the Clavicipitaceae. The LAH-producing Aspergillus species may be useful for study and production of these pharmaceutically important compounds.

opencc-by-4.0Nov 2022View details →

SvfA, survival factor A, contributes to Aspergillus nidulans pathogenicity

Supplementary information-SvfA, survival factor A, contributes to Aspergillus nidulans pathogenicity

Fig. 7. The 13C in Cytotoxic shornephines and asterresins from the hydrothermal vent associated fungus Aspergillus terreus CXX-158-20

Fig. 7. The 13C NMR calculation results of stereoisomers 6A/6 B at the pcSseg-1/revtpssrevtpss level using the means of GIAO. (A) The structures of 6A/6 B. (B) 13C NMR calculation results. (C) Linear correlation curve of 6A/6 B.

Fig. 6. The 13C in Cytotoxic shornephines and asterresins from the hydrothermal vent associated fungus Aspergillus terreus CXX-158-20

Fig. 6. The 13C NMR calculation results of stereoisomers 5A/5 B at the pcSseg-1/revtpssrevtpss level using the means of GIAO. (A) The structures of 5A/5 B. (B) 13C NMR calculation results. (C) Linear correlation curve of 5A/5 B.

opennotspecifiedApr 2023View details →

Fig. 8. Ochrathinols A in Ochrathinols A and B, two pairs of sulfur-containing racemates from an Antarctic fungus Aspergillus ochraceopetaliformis SCSIO 05702 inhibit LPS-induced pro-inflammatory cytokines and NO production

Fig. 8. Ochrathinols A (±)-1 compensated the NAD+/NADH ratio induced by LPS. Cells were exposed to 0.1 μg/mL LPS and co-treated with compounds of (±)-1 (10 μM) for 24 h. Intracellular NAD+/NADH ratios were determined by NAD+/NADH assay kit. Data are shown as mean ± SD (n = 3). *P <0.05, ****P <0.001 vs. LPS group, n = 3. P value was assessed by two-tailed Student's t-test.

opennotspecifiedApr 2023View details →

Fig. 7 in Ochrathinols A and B, two pairs of sulfur-containing racemates from an Antarctic fungus Aspergillus ochraceopetaliformis SCSIO 05702 inhibit LPS-induced pro-inflammatory cytokines and NO production

Fig. 7. Anti-inflammation activity of ochrathinols A and B ((±)-1, (±)-2). Cells were exposed to 0.1 μg/mL LPS and co-treated with compounds of (+)-1, ()-1, (±)-1, (+)-2, ()-2, (±)-2 (10 μM) for 24 h, respectively. (A) (±)-1 reduced the release of LPS-induced IL-6, TNF-α, and MCP-1 in culture medium, n = 3. (B) qPCR analysis of pro-inflammatory cytokines genes (IL-1β, IL-6, TNF-α, and MCP-1) normalized by β-actin, n = 3. All data are presented as the mean ± SD of three independent experiments. ns, P> 0.05, *P <0.05, **P <0.01, ***P <0.005, ****P <0.001 vs. LPS group; #P <0.05, ##P <0.01, ###P <0.005, ####P <0.001 vs. control group, n = 3. P value was assessed by two-tailed Student's t-test.

Fig. 4 in The anti-TMV potency of the tobacco-derived fungus Aspergillus versicolor and its active alkaloids, as anti-TMV activity inhibitors

Fig. 4. Docking poses (left) and interactions (right) of compound 10 (yellow) at the binding site of TMV (blue, PDB code 2OM3). The ligand with TMV A chain amino GLY-135 (1.92 Å) form three hydrogen bonds (green dotted line), Hydrogen bonding distance had been hidden for clarity. The binding energy of compound 10 for between ligands and TMV. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 2. The key 1H–1H in The anti-TMV potency of the tobacco-derived fungus Aspergillus versicolor and its active alkaloids, as anti-TMV activity inhibitors

Fig. 2. The key 1H–1H COSY (bold lines), HMBC (solid arrows), and ROESY (dashed arrows) correlations of compounds 1–5.

Fig. 3 in The anti-TMV potency of the tobacco-derived fungus Aspergillus versicolor and its active alkaloids, as anti-TMV activity inhibitors

Fig. 3. Docking poses (left) and interactions (right) of compound 1 (yellow) at the binding site of TMV (blue, PDB code 2OM3). The ligand with TMV. A chain amino ASN-73 (2.81 Å) and TYR-139 (3.04 Å) form four hydrogen bonds (green dotted line), Hydrogen bonding distance had been hidden for clarity. The binding energy of strong affinity between ligands and TMV. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article

opennotspecifiedAug 2023View details →

FIGURE 3. Aspergillus guangdongensis BCRC 32546 in New insights into Aspergillus guangdongensis (Aspergillaceae) and its first global record since the original description

FIGURE 3. Aspergillus guangdongensis BCRC 32546. (A–D) Colonies after 7 d at 25°C: (A) obverse CYA; (B) reverse CYA; (C) obverse MEA; (D) reverse MEA; (E–H) Conidiophores; (I–L) Conidia. (Bar = 10 µm). Photographs by Y.H. Wei.

opennotspecifiedAug 2023View details →

FIGURE 1 in New insights into Aspergillus guangdongensis (Aspergillaceae) and its first global record since the original description

FIGURE 1. Phylogenetic tree of Aspergillus section Ochraceorosei and related species, generated through maximum likelihood analysis of concatenated ITS, BenA, CaM, and RPB2 gene sequences. Bootstrap values based on 1000 replicates only exceeding 50% are indicated above or under branch nodes. Bar scale represents 0.05 substitutions per nucleotide position. Type strains are denoted by "T," while the new record strains of Aspergillus guangdongensis are highlighted in bold.

opennotspecifiedMay 2021View details →

Fig. 4 in Prenylated phenylbutyrolactones from cultures of a marine sponge-associated fungus Aspergillus flavipes KUFA1152

Fig. 4. Proposed biosynthetic pathways for the formation of 5 and 7 through a dichotomy of prenylation of 3 by dimethylallyl pyrophosphate (DMAPP), catalyzed by prenyltransferases.