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opencc-zeroNov 2014View details →

Naive B cells, Antibody secreting cells, Plasma cells

Raw Illumina MiSeq data in zipped FASTQ format. The data set includes demultiplexed samples from naive B cells (_20433_), antibody secreting cells (_20434_) and plasma cells (_20435_) obtained from the same Balb/c mouse via sorting. Every sample consists of forward (_R1_), reverse (_R2_)  reads (2x250 bp).

opencc-by-sa-4.0Aug 2015View details →

Immune repertoire profiling reveals that clonally expanded B and T cells infiltrating diseased human kidneys can also be tracked in the blood

Recent advances in high-throughput sequencing allow for the competitive analysis of the human B and T cell immune repertoire. In this study we compared Immunoglobulin and T cell receptor repertoires of lymphocytes found in kidney and blood samples of 10 patients with various renal diseases based on next-generation sequencing data.

opencc-by-4.0Sep 2024View details →

Distinct Stromal Cell Populations Define the B-cell Acute Lymphoblastic Leukemia Microenvironment

Processed single-cell RNA-seq from the study  <ul> <li>10X Genomics CellRanger output (barcodes.tsv, genes.tsv, matrix.mtx) for each each sample</li> <li>Metadata</li> <li>Seurat object of the integrated scRNAseq dataset</li> <li>Xenium object of the spatial transcriptomic data</li> </ul> Distinct Stromal Cell Populations Define the B-cell Acute Lymphoblastic Leukemia Microenvironment Mauricio N. Ferrao Blanco1, Bexultan Kazybay1, Mirjam Belderbos1, Olaf Heidenreich1, Hermann Josef Vormoor1,2 1 Princess Máxima Center for Pediatric Oncology, Utrecht, the Netherlands 2 University Medical Center Utrecht, Utrecht, the Netherlands Abstract The bone marrow microenvironment plays a critical role in B-cell acute lymphoblastic leukemia (B-ALL) progression, yet its cellular heterogeneity remains poorly understood. Using single-cell RNA sequencing on patient-derived of bone marrow aspirates from pediatric B-ALL patients, we identified two distinct mesenchymal stromal cell (MSC) populations: early mesenchymal progenitors and adipogenic progenitors. Spatial transcriptomic analysis further revealed the localization of these cell types and identified a third stromal population, osteogenic-lineage cells, exclusively present in the bone biopsy. Functional ex vivo assays using sorted stromal populations derived from B-ALL patient bone marrow aspirates demonstrated that both early mesenchymal and adipogenic progenitors secrete key niche-supportive factors, including CXCL12 and Osteopontin, and support leukemic cell survival and chemoresistance. Transcriptomic profiling revealed that B-ALL cells interact differently with stromal subtypes. Notably, adipogenic progenitors, but not early mesenchymal progenitors, provide support to leukemic cells through interleukin-7 and VCAM1 signaling. Stromal cells from B-ALL patients exhibited an enhanced adipogenic differentiation capacity compared to healthy controls. Moreover, co-culture experiments showed that B-ALL cells induce adipogenic differentiation in healthy MSCs through a cell contact-dependent mechanism. Adipogenic progenitors were also enriched in relapse samples, implicating them in disease progression. These findings highlight the complexity of the B-ALL microenvironment and identify different specialized stromal niches with which the leukemic cells can engage.

Fig.ç6.Ec hinoderes ohtsukai sp. nov., holotype, male (ZIHU 3976), Nomarski photomicrographs. A, Segments 1 and 2, ventral view; B, segments 4 and 5, ventral view. Abbreviations: dss, droplet-shaped sensory spot; gco1, glandular cell outlet type I; gco2, modi ed glandular cell outlet type II; lvt, lateroventral tubule; pac, pachycyclus; pf, pectinate fringe; rss, rounded sensory spot. in A New Brackish-water Species of Echinoderes (Kinorhyncha: Cyclorhagida) from the Seto Inland Sea, Japan

Fig.ç6.Ec hinoderes ohtsukai sp. nov., holotype, male (ZIHU 3976), Nomarski photomicrographs. A, Segments 1 and 2, ventral view; B, segments 4 and 5, ventral view. Abbreviations: dss, droplet-shaped sensory spot; gco1, glandular cell outlet type I; gco2, modi ed glandular cell outlet type II; lvt, lateroventral tubule; pac, pachycyclus; pf, pectinate fringe; rss, rounded sensory spot.

Fig.ç5.Ec hinoderes ohtsukai sp. nov., holotype, male (ZIHU 3976), Nomarski photomicrographs. A, Segments 1 and 2, dorsal view; B, segment 4, dorsal view. Abbreviations: dss, droplet-shaped sensory spot; gco1, glandular cell outlet type I; gco2, modi ed glandular cell outlet type II; mds, middorsal spine; pac, pachycyclus; pf, pectinate fringe; ps, perforation site; rss, rounded sensory spot. in A New Brackish-water Species of Echinoderes (Kinorhyncha: Cyclorhagida) from the Seto Inland Sea, Japan

Fig.ç5.Ec hinoderes ohtsukai sp. nov., holotype, male (ZIHU 3976), Nomarski photomicrographs. A, Segments 1 and 2, dorsal view; B, segment 4, dorsal view. Abbreviations: dss, droplet-shaped sensory spot; gco1, glandular cell outlet type I; gco2, modi ed glandular cell outlet type II; mds, middorsal spine; pac, pachycyclus; pf, pectinate fringe; ps, perforation site; rss, rounded sensory spot.

Fig.ç7.Ec hinoderes ohtsukai sp. nov., paratype, female (ZIHU 3980), Nomarski photomicrographs. A, Segments 5 and 6, ventral view; B, segments 8 and 9, ventral view. Abbreviations: dss, droplet-shaped sensory spot; gco2, modi ed glandular cell outlet type II; lvt, lateroventral tubule; si, sieve plate; sp, sternal plate; tp, tergal plate. in A New Brackish-water Species of Echinoderes (Kinorhyncha: Cyclorhagida) from the Seto Inland Sea, Japan

Fig.ç7.Ec hinoderes ohtsukai sp. nov., paratype, female (ZIHU 3980), Nomarski photomicrographs. A, Segments 5 and 6, ventral view; B, segments 8 and 9, ventral view. Abbreviations: dss, droplet-shaped sensory spot; gco2, modi ed glandular cell outlet type II; lvt, lateroventral tubule; si, sieve plate; sp, sternal plate; tp, tergal plate.

Fig.ç3.Ec hinoderes ohtsukai sp. nov., scanning electron micrographs. A, B, Paratype, female (ZIHU 3983); C–E, paratype, male (ZIHU 3982). A, General habitus, lateral view; B, neck and segments 1–4, lateral view; C, enlargement of segment 7, lateral view; D, enlargement of segment 9, lateral view; E, enlargement of segments 10 and 11, lateroventral view. Abbreviations: ch, cuticular hair; dss, droplet-shaped sensory spot; gco2, modi ed glandular cell outlet type II; ldt, laterodorsal tubule; pf, pectinate fringe; po, pore; ps1, penile spine 1; ps2, penile spine 2; ps3, penile spine 3; rss, rounded sensory spot; si, sieve plate; ss, sensory spot. in A New Brackish-water Species of Echinoderes (Kinorhyncha: Cyclorhagida) from the Seto Inland Sea, Japan

Fig.ç3.Ec hinoderes ohtsukai sp. nov., scanning electron micrographs. A, B, Paratype, female (ZIHU 3983); C–E, paratype, male (ZIHU 3982). A, General habitus, lateral view; B, neck and segments 1–4, lateral view; C, enlargement of segment 7, lateral view; D, enlargement of segment 9, lateral view; E, enlargement of segments 10 and 11, lateroventral view. Abbreviations: ch, cuticular hair; dss, droplet-shaped sensory spot; gco2, modi ed glandular cell outlet type II; ldt, laterodorsal tubule; pf, pectinate fringe; po, pore; ps1, penile spine 1; ps2, penile spine 2; ps3, penile spine 3; rss, rounded sensory spot; si, sieve plate; ss, sensory spot.

Fig.ç2.Ec hinoderes ohtsukai sp. nov., camera lucida drawings. A, B, Holotype, male (ZIHU 3976), entire animal, dorsal and ventral view, respectively; C, D, allotype, female (ZIHU 3977), segments 9–11, dorsal and ventral view, respectively. Abbreviations: dss, droplet-shaped sensory spot; gco1, glandular cell outlet type I; gco2, modi ed glandular cell outlet type II; ldt, laterodorsal tubule; lts, lateral terminal spine; lvt, lateroventral tubule; mds, middorsal spine; ne, neck; ps, penile spine; rss, rounded sensory spot; si, sieve plate. in A New Brackish-water Species of Echinoderes (Kinorhyncha: Cyclorhagida) from the Seto Inland Sea, Japan

Fig.ç2.Ec hinoderes ohtsukai sp. nov., camera lucida drawings. A, B, Holotype, male (ZIHU 3976), entire animal, dorsal and ventral view, respectively; C, D, allotype, female (ZIHU 3977), segments 9–11, dorsal and ventral view, respectively. Abbreviations: dss, droplet-shaped sensory spot; gco1, glandular cell outlet type I; gco2, modi ed glandular cell outlet type II; ldt, laterodorsal tubule; lts, lateral terminal spine; lvt, lateroventral tubule; mds, middorsal spine; ne, neck; ps, penile spine; rss, rounded sensory spot; si, sieve plate.

opencc-by-4.0Mar 2024View details →

Double-negative B cells and DNASE1L3 colocalise with microbiota in gut-associated lymphoid tissue - IMC+RNAScope images

Intestinal homeostasis is maintained by the response of gut-associated lymphoid tissue to bacteria transported across the follicle associated epithelium into the subepithelial dome. The initial response to antigens and how bacteria are handled is incompletely understood. By iterative application of spatial transcriptomics and multiplexed single-cell technologies, we identify that the double negative 2 subset of B cells, previously associated with autoimmune diseases, is present in the subepithelial dome in health. We show that in this location double negative 2 B cells interact with dendritic cells co-expressing the lupus autoantigens DNASE1L3 and C1q and microbicides.  We observe that in humans, but not in mice, dendritic cells expressing DNASE1L3 are associated with sampled bacteria but not DNA derived from apoptotic cells. We propose that fundamental features of autoimmune diseases are microbiota-associated, interacting components of normal intestinal immunity.

opencc-by-4.0Nov 2024View details →

Tumor and Blood B Cell Abundance Outperforms Established ICB Response Prediction Signatures in Head and Neck Cancer

<div> <div> <div> <div> This dataset contains processed flow cytometry data and clinical information for deidentified patients from Cohort 11, as well as deconvoluted cell abundances and clinical data for deidentified patients from Cohort 10, associated with the study titled "Tumor and Blood B Cell Abundance Outperforms Established Immune Checkpoint Blockade Response Prediction Signatures in Head and Neck Cancer" published in Annals of Oncology (2024). <a href="https://doi.org/10.1016/j.annonc.2024.11.008" target="_new" rel="noopener">DOI: https://doi.org/10.1016/j.annonc.2024.11.008</a>. </div> </div> </div> </div> <div> <div> <div> </div> </div> </div>

opencc-by-4.0Oct 2024View details →

Memory B Cells and Their Transcriptomic Profiles Associated with Belimumab Resistance in Systemic Lupus Erythematosus in the Maintenance Phase

Normalized count of RNA sequences from systemic lupus erythematosus patients treated with belimumab. We collected blood samples from patients with systemic lupus erythematosus (n=44) before and approximately 3 and 6 months after treatment by belimumab, respectively. We also included healthy individuals (n=17). There were no missing samples in the before-treatment specimen for 44 patients; however, five samples were missing at the three-month time, and four were missing at the six-month time after treatment. Whole blood samples were stored in PAXgene tubes (QUIAGEN). RNA was extracted by PAXgene Blood RNA Kit (QUIAGEN). Library preparation was performed using TruSeq stranded Total RNA Library PrepKit with Ribo-Zero Globin Human. Sequencing was conducted by NovaSeq6000 in the 100-bp paired-end mode. Sequencing reads were trimmed using Trimmomatic ver. 0.36 (leading: 20, trailing: 20, slidingwindow: 4:15, minlen: 36) and aligned to hg38 reference genome using STAR (ver. 2.7.3a). Gene counts were generated by RSEM (ver. 1.3.1) using Homo_sapiens.GRCh38.95.gtf from the Ensembl database. Gene counts were normalized by size factor implemented in DESeq2 and converted to count per million (CPM), and log2(CPM+1) was calculated.  Sample names starting with 'R_' indicate responders, while those ending with '_NR' indicate non-responders. '0M' represents before treatment, '3M' represents three months after treatment, and '6M' represents six months after treatment. The numeric ID, excluding the last two characters, represents individual patients.   If you use this data, please cite Iwasaki et al. Front. Immunol., 04 February 2025 <a href="https://doi.org/10.3389/fimmu.2025.1506298">https://doi.org/10.3389/fimmu.2025.1506298</a>

opencc-by-4.0Nov 2021View details →

Pre-vaccination and early B cell signatures of the antibody response to SARS-CoV-2 mRNA vaccine

The data presented in Code repository for Kardava, L., Rachmaninoff, N., Lau, W. W., Buckner, C. M., Trihemasava, K., Blazkova, J., ... & Moir, S. (2022). Early human B cell signatures of the primary antibody response to mRNA vaccination. Proceedings of the National Academy of Sciences, 119(28), e2204607119.<a href="https://www.pnas.org/doi/epdf/10.1073/pnas.2204607119">https://www.pnas.org/doi/epdf/10.1073/pnas.2204607119</a> are made available here. All code to reproduce the figures can be found here: https://github.com/niaid/COVID_Vaccine_Bcells <a href="https://zenodo.org/api/files/f93859d0-b062-4def-8b17-c0f21ee36f09/all_subjects_cd19_positive_and_keys.zip">all_subjects_cd19_positive_and_keys.zip</a> contains a CSV file of all CD19+ cells with flowSOM clusters shown. Accompanying files allow for matching of timepoint and subject information. <a href="https://zenodo.org/api/files/f93859d0-b062-4def-8b17-c0f21ee36f09/All_subjects_FCS_files_deidentified.zip">All_subjects_FCS_files_deidentified.zip</a> contains the raw fcs files and is organized by timepoint and subject.

opencc-by-4.0Jan 2022View details →

Germinal centre-driven maturation of B cell response to SARS-CoV-2 mRNA vaccination

These are the processed BCR repertoire and transcriptomics data described in <a href="https://doi.org/10.1038/s41586-022-04527-1">Kim & Zhou et al., Nature, 2022</a>. The raw sequencing data new to this study are available on SRA under BioProject <a href="https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA777934">PRJNA777934</a>. This study also used BCR repertoire data from <a href="https://doi.org/10.1038/s41586-021-03738-2">Turner & O'Halloran et al., Nature, 2021</a> (<a href="https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA731610">PRJNA731610</a>) and <a href="https://doi.org/10.1016/j.immuni.2021.08.013">Schmitz, Turner & Liu et al., Immunity, 2021</a> (<a href="https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA741267">PRJNA741267</a>).   Code Code along with Docker containers for reproducing the NGS data-based figures and analyses in the published paper can be <a href="https://github.com/julianqz/wustl_published/tree/main/nature_2022">found on GitHub</a>.   Metadata File: WU368_kim_et_al_nature_2022_meta.tsv Notes: <ul> <li>Sample breakdown by `sequence_type` (132 total) <ul> <li>73 bulk BCR sequencing samples (`bulk`) <ul> <li>57 new</li> <li>5 from <a href="https://doi.org/10.1038/s41586-021-03738-2">Turner & O'Halloran et al., Nature, 2021</a></li> <li>11 from <a href="https://doi.org/10.1016/j.immuni.2021.08.013">Schmitz, Turner & Liu et al., Immunity, 2021</a>.</li> </ul> </li> <li>56 10x Genomics single-cell VDJ + 5' gene expression samples (`tgx`)</li> <li>3 samples from <a href="https://doi.org/10.1038/s41586-021-03738-2">Turner & O'Halloran et al., Nature, 2021</a> (`mab`) corresponding to a total of 37 S-binding mAbs previously reported. These are not the same as the 2099 recombinant mAbs generated in this study (see below).</li> </ul> </li> <li>Sample collection time was originally recorded in days in the `timepoint` column. Timepoints were referenced in weeks in the manuscript, as shown in the `timepoint_ms` column.</li> <li>`bio_rep` and `tech_rep` = biological replicate and technical replicate respectively.</li> </ul> Abbreviations: <ul> <li>LN = lymph node</li> <li>BM = bone marrow</li> <li>PB = plasmablast</li> <li>GC = germinal centre</li> <li>LLPC = long-lived plasma cell</li> <li>NS = no sorting</li> <li>mAb = monoclonal antibody</li> </ul>   Information on the 2099 recombinant mAbs generated in this study File: WU368_kim_et_al_nature_2022_mabs.tsv Notes on columns: <ul> <li>`h_sequence_id` and `l_sequence_id`: Sequence IDs of the heavy and light chains respectively.</li> <li>`elisa`: ELISA results for binding to SARS-CoV-2 S (`TRUE` = positive).</li> </ul>   Processed BCR data - heavy chains File: WU368_kim_et_al_nature_2022_bcr_heavy.tsv Analysis was based on heavy chain-based clonal inference. Notes on columns: The columns largely follow the <a href="https://changeo.readthedocs.io/en/stable/standard.html">AIRR-C Rearrangement format</a>. The main deviation is that CDR3s were used, as opposed to IMGT-defined "junctions". Nonetheless, junction-related columns are included here as some repositories such as <a href="https://gateway.ireceptor.org/login">iReceptor</a> use these. Non-standard columns are noted below. <ul> </ul> <ul> <li>`cell_id`: Only sequences from single-cell samples and the 37 mAbs from Turner & O'Halloran et al., Nature, 2021 have cell IDs following the format `[donor]_[sample]@[id]`. `NA` for bulk sequences.</li> <li>`sequence_id`: Sequence IDs follow the format `[donor]_[sample]@[id]`.</li> <li>`v_call_genotyped`: V gene annotation reassigned after individualized genotyping by <a href="https://tigger.readthedocs.io/en/stable/">TIgGER</a>.</li> <li>`germline_[vdj]_call`: Clonal consensus germline calls after corresponding clonal consensus sequence were reconstructed via <a href="https://changeo.readthedocs.io/en/stable/methods/germlines.html">`CreateGermlines.py --cloned` from Change-O</a>.</li> <li>`isotype`: IGH[ADEGM].</li> <li>`cdr3`: CDR3 nucleotide sequence.</li> <li>`cdr3_length`: CDR3 nucleotide sequence length.</li> <li>`cdr3_aa`: CDR3 amino acid sequence.</li> <li>`collapse_count`: Number of duplicate IMGT-aligned V(D)J sequences that were collapsed by <a href="https://alakazam.readthedocs.io/en/stable/topics/collapseDuplicates/">`alakazam::collapseDuplicates`</a>.</li> <li>`donor`, `timepoint`, `tissue`, `sorting`, `seq_type`: Propagated as is from the metadata file. <ul> <li>In `seq_type`, `tgx` corresponds to 10x Genomics data; `mab` corresponds specifically to the 37 S-binding mAbs from Turner & O'Halloran et al., Nature, 2021.</li> </ul> </li> <li>`timepoint_2`: Same as `timepoint`, except that `d28+d35` and `d201+d208` were treated as `d28` (week 4) and `d201` (week 29) respectively as described in Materials & Methods.</li> <li>`gex_anno`: Cell type identity annotation based on transcriptomic profiles. Mapped from `anno_leiden_0.18` from WU368_kim_et_al_nature_2022_gex_b_cells.h5ad.</li> <li>`compartment`: B cell compartment. <ul> <li>ABC = activated B cell. LNPC = lymph node plasma cell. RMB = resting memory B cell.</li> <li>Minor differences in terminology <ul> <li>The manuscript refers to the memory compartment as MBCs, whereas the terminology used in the data is RMB. As described in Materials & Methods, analysis involving the memory compartment used specifically d201 bulk-sequenced memory sorts from blood. To get these sequences, subset `s_pos_clone`, `seq_type`, `compartment`, and `timepoint_2` to, respectively, `TRUE`, `bulk`, `RMB`, and `d201`. </li> <li>The manuscript uses the term BMPC (bone marrow plasma cell), whereas the data uses the term LLPC.</li> </ul> </li> </ul> </li> <li>`clone_id`: B cell clonal lineage IDs follow the format `[donor]@[id]`.</li> <li>`s_pos_clone`: `TRUE` if a sequence belonged to a B cell clone that was designated as S-binding by virtue of containing one of the recombinant mAbs that tested positive via ELISA or one of the S-binding mAbs from Turner & O'Halloran et al., Nature, 2021.</li> <li>`expressed_id`: mAb IDs for the 2099 recombinant mAbs generated in this study (mapped from `mab_id` from WU368_kim_et_al_nature_2022_mabs.tsv) and the 37 mAbs from Turner & O'Halloran et al., Nature, 2021. `NA` for everything else.</li> <li>`elisa`: ELISA results for binding of recombinant mAbs to SARS-CoV-2 S. `TRUE` if positive. `NA` if not tested.</li> <li>`nuc_RS_19_312`: number of replacement and silent mutations between IMGT-numbered nucleotide positions 19-312 along IGHV sequences, calculated by <a href="https://shazam.readthedocs.io/en/stable/topics/calcObservedMutations/">`shazam::calcObservedMutations`</a>.</li> <li>`nuc_denom_19_312`: number of informative nucleotide positions for counting mutations, excluding non-A/T/G/C positions (such as "N", "-", ".").</li> <li>`nuc_RS_freq_19_312`: nucleotide-level mutation frequency (= nuc_RS_19_312 / nuc_denom_19_312).</li> </ul>   Processed BCR data - light chains File: WU368_kim_et_al_nature_2022_bcr_light.tsv Light chains were not used for heavy chain-based clonal inference or analysis.   Processed transcriptomics data Files: <ul> <li>WU368_kim_et_al_nature_2022_gex_all_cells.h5ad (clustering all cells)</li> <li>WU368_kim_et_al_nature_2022_gex_b_cells.h5ad (re-clustering only the B cells)</li> </ul> Notes: <ul> <li>The `h5ad` files can be imported into <a href="https://scanpy.readthedocs.io/en/stable/index.html">Scanpy</a> as an <a href="https://scanpy.readthedocs.io/en/stable/usage-principles.html#anndata">AnnData object</a>.</li> <li>Each `AnnData` object has 3 `.layers`, each representing a version of the count matrix. <ul> <li>`raw_counts`: Imported from `<a href="https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/6.0/using/aggregate">cellranger aggr</a>` output by `scanpy.read_10x_mtx`.</li> <li>`log_norm`: Log-noramlized expression values outputted by `scanpy.pp.normalize_total` followed by `scanpy.pp.log1p`.</li> <li>`scaled`: The `log_norm` layer scaled to unit variance and zero mean by `scanpy.pp.scale`. </li> </ul> </li> <li>The `gene_name` and `biotype` columns in `.var` were extracted from GENCODE v32 GTF.</li> <li>Columns in `.obs` (each row corresponds to a cell) <ul> <li>`n_feature`: The `n_genes_by_counts` column produced by `scanpy.pp.calculate_qc_metrics`, renamed. The number of genes expressed. This is before subsetting the genes.</li> <li>`n_umi`: The `total_counts` column produced by `scanpy.pp.calculate_qc_metrics`, renamed. The total UMI counts in a cell.</li> <li>`pct_mt`: The `pct_counts_mt` column produced by `scanpy.pp.calculate_qc_metrics`, renamed. The percentage of counts in mitochondrial genes.</li> <li>`n_hkg`: The number of housekeeping genes for which expression was detected.</li> <li>`n_gene_expressed`: The total number of genes for which expression was detected. This is after subsetting the genes.</li> <li>`pre_qc_bcr`: `TRUE` if a cell also had paired BCR data available. Produced by cross-referencing the cellular barcodes in `cell_barcodes.json` outputted by `cellranger vdj`. At this point the BCR data had not gone through the QC process in the BCR processing pipeline (hence `pre_qc`). </li> <li>`leiden_[resolution]`: Cluster assignment by `scanpy.tl.leiden`.</li> <li>`anno_leiden_[resolution]`: Cell type identity annotations based on transcriptomic profiles. This was mapped onto the `gex_anno` column in the processed heavy chain BCR data.</li> </ul> </li> <li>UMAP coordinates can be found in `.obsm["X_umap"]`.</li> <li>`.X` has been set to `None` in order to reduce file size.</li> </ul> In addition, the preprocessed count matrix outputted by `<a href="https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/6.0/using/aggregate">cellranger aggr</a>` is available from <a href="https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE195673">GEO under BioProject PRJNA777934</a>.

opencc-by-4.0Dec 2021View details →

Text-fig. 7. Scanning electron micrographs (a, b, d, e, g–k), X-ray microtomographic orthoslices (c) and synchrotron radiation X-ray tomographic microscopy orthoslices (f) of fruits and endocarps of uncertain affinity from Zliv-Řídká Blana locality. a–c: Trebecenia sarcocalis, a – tricarpellate fruit, no. NM-F 3637, b – fruits supported by pentamerous and persistent calyx, no. NMF 3637, c – fruit almost circular in transverse section, no. NM-F 3637; d: Taxon 17, small fruit with slightly sunken stylar region, no. NM-F 3201; e: Taxon 19, spherical fruit, the fruit wall composed of large isodiametric, thick walled cells, no. NM-F 3181; f: Taxon 19, single-seeded fruit, no. NM-F 3621; g: Taxon 20, syncarpous, multicarpellate fruit of ten carpels, no. NM-F 3200; h: Taxon 22, syncarpous, multicarpellate fruit of seven carpels, no. NM-F 3159; i: cf. Sabia menispermoides, endocarp of drupaceous fruits, no. NM-F 4624; j: Taxon 25, endocarp triangular in cross-section, no. NM-F 3218; k: Taxon 24, endocarp spherical in cross-section with a distinctly ribbed and foveolate surface, no. NM-F 3217. in Plant Mesofossils From The Late Cretaceous Klikov Formation, The Czech Republic

Text-fig. 7. Scanning electron micrographs (a, b, d, e, g–k), X-ray microtomographic orthoslices (c) and synchrotron radiation X-ray tomographic microscopy orthoslices (f) of fruits and endocarps of uncertain affinity from Zliv-Řídká Blana locality. a–c: Trebecenia sarcocalis, a – tricarpellate fruit, no. NM-F 3637, b – fruits supported by pentamerous and persistent calyx, no. NMF 3637, c – fruit almost circular in transverse section, no. NM-F 3637; d: Taxon 17, small fruit with slightly sunken stylar region, no. NM-F 3201; e: Taxon 19, spherical fruit, the fruit wall composed of large isodiametric, thick walled cells, no. NM-F 3181; f: Taxon 19, single-seeded fruit, no. NM-F 3621; g: Taxon 20, syncarpous, multicarpellate fruit of ten carpels, no. NM-F 3200; h: Taxon 22, syncarpous, multicarpellate fruit of seven carpels, no. NM-F 3159; i: cf. Sabia menispermoides, endocarp of drupaceous fruits, no. NM-F 4624; j: Taxon 25, endocarp triangular in cross-section, no. NM-F 3218; k: Taxon 24, endocarp spherical in cross-section with a distinctly ribbed and foveolate surface, no. NM-F 3217.

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Text-fig. 6. Scanning electron micrographs of seeds of ericalean affinity (a–d) and seeds of uncertain affinity (e–i) from Zliv-Řídká Blana locality. a: Protovisnea sp. 1, rounded seed with the narrow elongate seed cavity flanked by two bulging regions of larger cells, no. NM-F 3177; b: Protovisnea sp. 2, angular seed with the narrow elongate seed cavity flanked by two bulging regions of larger cells, no. NM-F 3179; c, d: Eurya crassitesta, one seed split into two parts, no. NM-F 3211, c – surface cells of the seed coat are palisade, d – cross-section of the seed; e: Nympheaceae sp. 1, seed, no. NM-F 3636; f: Nympheaceae sp. 2, seed, no. NM-F 4634; g: Klikovispermum sp.1, seeds with irregular outline and smooth outer surface, no. NM-F 3203; h: Klikovispermum malechii, seed with an orange-segment shape, no. NM-F 3299; i: Taxon 35, seed, no. NM-F 3236. in Plant Mesofossils From The Late Cretaceous Klikov Formation, The Czech Republic

Text-fig. 6. Scanning electron micrographs of seeds of ericalean affinity (a–d) and seeds of uncertain affinity (e–i) from Zliv-Řídká Blana locality. a: Protovisnea sp. 1, rounded seed with the narrow elongate seed cavity flanked by two bulging regions of larger cells, no. NM-F 3177; b: Protovisnea sp. 2, angular seed with the narrow elongate seed cavity flanked by two bulging regions of larger cells, no. NM-F 3179; c, d: Eurya crassitesta, one seed split into two parts, no. NM-F 3211, c – surface cells of the seed coat are palisade, d – cross-section of the seed; e: Nympheaceae sp. 1, seed, no. NM-F 3636; f: Nympheaceae sp. 2, seed, no. NM-F 4634; g: Klikovispermum sp.1, seeds with irregular outline and smooth outer surface, no. NM-F 3203; h: Klikovispermum malechii, seed with an orange-segment shape, no. NM-F 3299; i: Taxon 35, seed, no. NM-F 3236.

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Text-fig. 2. Scanning electron micrographs of non-angiosperm remains (a–d) and insect remains (e–f) from Zliv-Řídká Blana locality. a: Eopolytrichium sp. small leafy shoot, no. NM-F 3631; b: Taxon 1, single tip of a young fern frond with circinate vernation, no. NM-F 4130; c: Taxon 2, fern with simple leaves and circinate vernation, no. NM-F 3459; d: Pagiophyllum sp., small needle-like leaf, no. NM-F 4132; e: Microcarpolithes hexagonalis, a faecal pellet/coprolite with subcylindrical shape, no. NMF 4520; f: Palaeoaldrovanda splendens, pieces of compact walls formed by rectangular cells, no. NM-F 3237. in Plant Mesofossils From The Late Cretaceous Klikov Formation, The Czech Republic

Text-fig. 2. Scanning electron micrographs of non-angiosperm remains (a–d) and insect remains (e–f) from Zliv-Řídká Blana locality. a: Eopolytrichium sp. small leafy shoot, no. NM-F 3631; b: Taxon 1, single tip of a young fern frond with circinate vernation, no. NM-F 4130; c: Taxon 2, fern with simple leaves and circinate vernation, no. NM-F 3459; d: Pagiophyllum sp., small needle-like leaf, no. NM-F 4132; e: Microcarpolithes hexagonalis, a faecal pellet/coprolite with subcylindrical shape, no. NMF 4520; f: Palaeoaldrovanda splendens, pieces of compact walls formed by rectangular cells, no. NM-F 3237.

Text-fig. 10. Platanoxylon cf. haydenii, a, e, h: UF 279-34470; b, c, d, f g: UF 279-34469. a, b: Diffuse porous wood with vessels solitary and in small multiples, which are mostly tangential or oblique, diffuse and diffuse-in-aggregates axial parenchyma., TS. c–e: Scalariform perforation plates. f, g: Opposite intervessel pits, TLS. h: Two size classes of rays, TLS. Platanus sp., UF 279- 24552. i: Predominantly solitary vessels, diffuse and diffuse-in-aggregates parenchyma, growth ring boundary distinct, noded rays, TS. j: Simple perforation plates (PP), RLS. k: Body of ray with procumbent ray cells, RLS. l: Scalariform perforation plate, RLS. m: Rays of two sizes, wide rays>10-seriate, TLS. Scale bars: 200 µm in a, b, h, i, m; 100 µm in j, k: 50 µm in c, d, e, f, l. in A Diverse Assemblage Of Late Eocene Woods From Oregon, Western Usa

Text-fig. 10. Platanoxylon cf. haydenii, a, e, h: UF 279-34470; b, c, d, f g: UF 279-34469. a, b: Diffuse porous wood with vessels solitary and in small multiples, which are mostly tangential or oblique, diffuse and diffuse-in-aggregates axial parenchyma., TS. c–e: Scalariform perforation plates. f, g: Opposite intervessel pits, TLS. h: Two size classes of rays, TLS. Platanus sp., UF 279- 24552. i: Predominantly solitary vessels, diffuse and diffuse-in-aggregates parenchyma, growth ring boundary distinct, noded rays, TS. j: Simple perforation plates (PP), RLS. k: Body of ray with procumbent ray cells, RLS. l: Scalariform perforation plate, RLS. m: Rays of two sizes, wide rays>10-seriate, TLS. Scale bars: 200 µm in a, b, h, i, m; 100 µm in j, k: 50 µm in c, d, e, f, l.

Text-fig. 9. Wataria kvacekii sp. nov., UF 279-24556. a: Wood ring-porous, earlywood with 2–3 rows of wide pores, vessels solitary and in radial multiples of 2, axial parenchyma scanty vasicentric and some apotracheal diffuse-in-aggregates, TS. b: Series of vessel elements with simple perforations, axial parenchyma strands adjacent to vessels, RLS. c: Alternate intervessel pitting, vessel element end walls horizontal, RLS. d: Vessel-axial parenchyma pitting similar to intervessel pitting, RLS. e, f: Rays with tile cells, storied axial parenchyma, some strands chambered crystalliferous, TLS. g: Detail of ray, TLS. h: Storied imperforate elements. Scale bars: 200 µm in a; 100 µm in b, e; 50 µm in c, d, f, h; 20 µm in g. in A Diverse Assemblage Of Late Eocene Woods From Oregon, Western Usa

Text-fig. 9. Wataria kvacekii sp. nov., UF 279-24556. a: Wood ring-porous, earlywood with 2–3 rows of wide pores, vessels solitary and in radial multiples of 2, axial parenchyma scanty vasicentric and some apotracheal diffuse-in-aggregates, TS. b: Series of vessel elements with simple perforations, axial parenchyma strands adjacent to vessels, RLS. c: Alternate intervessel pitting, vessel element end walls horizontal, RLS. d: Vessel-axial parenchyma pitting similar to intervessel pitting, RLS. e, f: Rays with tile cells, storied axial parenchyma, some strands chambered crystalliferous, TLS. g: Detail of ray, TLS. h: Storied imperforate elements. Scale bars: 200 µm in a; 100 µm in b, e; 50 µm in c, d, f, h; 20 µm in g.

Text-fig. 8. Pterocaryoxylon sp., a–c, e: UF 279-85024; d, f: UF 279-24551. a, b: Wood semi-ring-porous, vessels solitary and in short radial multiples, axial parenchyma scanty vasicentric, marginal, and in narrow lines, TS. c: Crowded alternate intervessel pitting, simple perforation plate (PP), TLS. d: Vessel-axial parenchyma pitting similar to intervessel pitting, RLS. e: Rays mostly 1–3 cells wide, occasionally 4 cells, uniseriate rays probably mostly square to upright cells, TLS. f: Rays heterocellular, body cells procumbent. Scale bars: 200 µm in a, b; 100 µm in e, f; 50 µm in c; 20 µm in d. in A Diverse Assemblage Of Late Eocene Woods From Oregon, Western Usa

Text-fig. 8. Pterocaryoxylon sp., a–c, e: UF 279-85024; d, f: UF 279-24551. a, b: Wood semi-ring-porous, vessels solitary and in short radial multiples, axial parenchyma scanty vasicentric, marginal, and in narrow lines, TS. c: Crowded alternate intervessel pitting, simple perforation plate (PP), TLS. d: Vessel-axial parenchyma pitting similar to intervessel pitting, RLS. e: Rays mostly 1–3 cells wide, occasionally 4 cells, uniseriate rays probably mostly square to upright cells, TLS. f: Rays heterocellular, body cells procumbent. Scale bars: 200 µm in a, b; 100 µm in e, f; 50 µm in c; 20 µm in d.

Text-fig. 7. Hamamelidoxylon crystalliferum sp. nov., UF 279-34464. a: Diffuse porous wood with exclusively solitary vessels, tending to be angular in outline, TS. b: Growth ring boundary, marked by radially narrowed fibers, latewood vessels narrower than earlywood vessels of the next ring, fibers thick-walled, no axial parenchyma visible, TS. c: Scalariform intervessel pits in narrow vessel (left), fibers with distinctly bordered pits, TLS. d: Scalariform perforation plate, tyloses (T) formation from marginal ray cell, TLS. e: Scalariform perforation plates with fewer than 15 bars (PP), RLS. f: Vessel-ray parenchyma pits with in A Diverse Assemblage Of Late Eocene Woods From Oregon, Western Usa

Text-fig. 7. Hamamelidoxylon crystalliferum sp. nov., UF 279-34464. a: Diffuse porous wood with exclusively solitary vessels, tending to be angular in outline, TS. b: Growth ring boundary, marked by radially narrowed fibers, latewood vessels narrower than earlywood vessels of the next ring, fibers thick-walled, no axial parenchyma visible, TS. c: Scalariform intervessel pits in narrow vessel (left), fibers with distinctly bordered pits, TLS. d: Scalariform perforation plate, tyloses (T) formation from marginal ray cell, TLS. e: Scalariform perforation plates with fewer than 15 bars (PP), RLS. f: Vessel-ray parenchyma pits with