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1,977 results for “CRISPR”
10k 1:1 Mixture of Jurkat and MM1S Cells, 5' v2.0, CRISPR Screening, Chromium X - BESCA
<p>This dataset is a BESCA processed version of the example resources found at https://www.10xgenomics.com/resources/datasets/10k-1-1-mixture-of-jurkat-and-mm1s-cells-5-v2-0-chromium-x-2-standard</p>
BLASTN results for CRISPR mini-array protospacers search
<p><strong>Supplementary file 1: BLASTN results for protospacers search</strong></p> <p>This file contains the output of the BLASTN search of the spacers in the same genomic partition. See materials and methods for more details regarding the BLASTN search. All sequences between two adjacent repeats (less than 60bp apart) were retrieved and marked with the first repeat coordinates, spacer coordinates, and “Array” flag. For all single repeat-like sequences, 30bp up/downstream as potential spacers and marked with the repeat coordinates, spacer coordinates, and “SingleUpstream” or “SingleDownstream” flags.</p>
Data from: Pooled genome-wide CRISPR activation screening for rapamycin resistance genes in Drosophila cells
<p>Loss-of-function and gain-of-function genetic perturbations provide valuable insights into gene function. In <em>Drosophila</em> cells, while genome-wide loss-of-function screens have been extensively used to reveal mechanisms of a variety of biological processes, approaches for performing genome-wide gain-of-function screens are still lacking. Here, we describe a pooled CRISPR activation (CRISPRa) screening platform in <em>Drosophila</em> cells and apply this method to both focused and genome-wide screens to identify rapamycin resistance genes. The screens identified three genes as novel rapamycin resistance genes: a member of SLC16 family of monocarboxylate transporters (<em>CG8468)</em>, a member of the lipocalin protein family (<em>CG5399</em>), and a zinc finger C2H2 transcription factor (<em>CG9932</em>). Mechanistically, we demonstrate that <em>CG5399</em> overexpression activates the RTK-Akt-mTOR signaling pathway and that activation of insulin receptor (InR) by <em>CG5399</em> requires cholesterol and clathrin-coated pits at the cell membrane. This study establishes a novel platform for functional genetic studies in <em>Drosophila</em> cells.</p>
Data from: A CRISPR-based rapid DNA repositioning strategy and the early intranuclear life of HSV-1
<p>The relative positions of viral DNA genomes to the host intranuclear environment play critical roles in determining virus fate. Recent advances in the application of chromosome conformation capture-based sequencing analysis (3C technologies) have revealed valuable aspects of the spatiotemporal interplay of viral genomes with host chromosomes. However, to elucidate the causal relationship between the subnuclear localization of viral genomes and the pathogenic outcome of an infection, manipulative tools are needed. Rapid repositioning of viral DNAs to specific subnuclear compartments amid infection is a powerful approach to synchronize and interrogate this dynamically changing process in space and time. Herein, we report an inducible CRISPR-based two-component platform that relocates extrachromosomal DNA pieces (5 kb to 170 kb) to the <strong>nu</strong>clear <strong>p</strong>eriphery <strong>in</strong> minutes (CRISPR-nuPin). Based on this strategy, investigations of herpes simplex virus 1 (HSV-1), a prototypical member of the human herpesvirus family, revealed unprecedently reported insights into the early intranuclear life of the pathogen: I) Viral genomes tethered to the nuclear periphery upon entry, compared with those freely infecting the nucleus, were wrapped around histones with increased suppressive modifications and subjected to stronger transcriptional silencing and prominent growth inhibition. II) Relocating HSV-1 genomes at 1 hour post infection significantly promoted the transcription of viral genes, termed an "Escaping" effect. III) Early accumulation of ICP0 was a sufficient but not necessary condition for "Escaping". IV) Subnuclear localization was only critical during early infection. Importantly, the CRISPR-nuPin tactic, in principle, is applicable to many other DNA viruses.</p>
Data from: CRISPR screening by AAV episome-sequencing (CrAAVe-seq): A scalable cell type-specific in vivo platform uncovers neuronal essential genes
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PAM-altering SNP-based allele-specific CRISPR-Cas9 therapeutic strategies for Huntington's disease
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Data from: Pooled genome-wide CRISPR activation screening for rapamycin resistance genes in Drosophila cells
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Development of an efficient CRISPR-mediated genome editing platform in the diploid-polyploid model system Tragopogon (Asteraceae)
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Diversity in CRISPR-based immunity protects susceptible genotypes by restricting phage spread and evolution
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Type IV-A3 CRISPR-Cas systems drive inter-plasmid conflicts by acquiring spacers in trans
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NGS data from: Detection of unintended on-target effects in CRISPR genome editing by DNA donors carrying diagnostic mutations
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Repurposing Type I-A CRISPR-Cas3 for a robust diagnosis of human papillomavirus (HPV)
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CRISPR-Cas9 screen upon Olaparib+CldU treatment
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RAD54L2 counters TOP2-DNA adducts to promote genome stability (Etoposide treated RPE1 CRISPR screens in TP53 and RAD53L2 knock outs)
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A novel CRISPR screen identifies mediators of cfDNA release
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Genetic editing of primary human dorsal root ganglion neurons using CRISPR-Cas9
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Simulation models from: Can CRISPR-mediated gene drive work in pest and beneficial haplodiploid species?
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Data from: Genome-wide CRISPR synthetic lethality screen identifies a role for the ADP-ribosyltransferase PARP14 in replication fork stability controlled by ATR
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Data from: Lung and liver editing using lipid nanoparticle delivery of a stable CRISPR-Cas9 RNP
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Data for: CRISPR spacers acquired from plasmids primarily target backbone genes, making them valuable for predicting potential hosts and host range
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.