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60 results for “Diaphorina citri”
Fig. 2 in Feeding behavior of Diaphorina citri (Hemiptera: Liviidae) and its acquisition of 'Candidatus Liberibacter asiaticus', on huanglongbing-infected Citrus reticulata leaves of several maturity stages
Fig. 2. Visual representation of EPG waveforms E1 (phloem salivation), E2 (phloem ingestion) and G (xylem ingestion) produced by adult Diaphorina citri feeding on HLB-infected Citrus sunki leaves.
Fig. 2 in Ultrastructure and development of the new stylets inside pre-molting first instar nymphs of the Asian citrus psyllid Diaphorina citri (Hemiptera: Liviidae)
Fig. 2. Transmission electron micrographs of thin sections in other organs of 1st instar nymphs of D. citri. A & B. The esophagus (A) and tracheal cells (B) of a pre-molting nymph; bl, basal lamina; ep, epithelial cells; nc, new cuticle; nt, new taenidia; nu, nucleus; oc, old cuticle, ot, old taenidia. C. Part of the filter chamber showing the chamber wall (cw), anterior midgut (amg) and posterior midgut (pmg); double arrows indicate closely apposed basal lamina of the anterior and posterior midgut; aml, anterior midgut lumen; mv, microvilli; pml, posterior midgut lumen. D. Bacteriocyte cell in the bacteriome with a large nucleus (nu) and various shaped electron-dense bacterial cells (ed). E. Another part of the bacteriome with 2 types of bacterial cells: electron-dense (ed) and electron-lucent (el).
Fig. 3 in Toxicity of an acetogenin-based bioinsecticide against Diaphorina citri (Hemiptera: Liviidae) and its parasitoid Tamarixia radiata (Hymenoptera: Eulophidae)
Fig. 3. Percentage of emergence of Tamarixia radiata adults exposed to ethanolic extract from Annona mucosa seeds (ESAM) during their larval stage. The concentration of extract used was equivalent to the LC90 (4,463.00 mg/L) estimated for D. citri adults (120 h of exposure).
Fig. 4 in Toxicity of an acetogenin-based bioinsecticide against Diaphorina citri (Hemiptera: Liviidae) and its parasitoid Tamarixia radiata (Hymenoptera: Eulophidae)
Fig. 4. Corrected mortality (Schneider-Orelli 1947) of Diaphorina citri adults exposed to residue on 'Valência' sweet orange seedlings treated with an aqueous emulsion of ethanolic extract from Annona mucosa seeds — ESAM (●) — or the commercial limonoid-based bioinsecticide Azamax® 1.2 EC (O) used as a positive control, at different periods afer application in a semi-field trial (greenhouse). The mortality levels were lower than 7.5% in the controls.
Fig. 1 in Toxicity of an acetogenin-based bioinsecticide against Diaphorina citri (Hemiptera: Liviidae) and its parasitoid Tamarixia radiata (Hymenoptera: Eulophidae)
Fig. 1. Effect of ethanolic extract from Annona mucosa seeds (ES- AM) on oviposition preference of copulated Diaphorina citri females (A) and on feeding in non-sexed D. citri adults (B). Both tests were conducted without opportunity to choose, and the concentration of the extract used was equivalent to the LC25 (224.92 mg/L) previously estimated for D. citri adults (120 h of exposure).
Fig. 2 in Toxicity of an acetogenin-based bioinsecticide against Diaphorina citri (Hemiptera: Liviidae) and its parasitoid Tamarixia radiata (Hymenoptera: Eulophidae)
Fig. 2. Filter paper discs (experimental units) of the control and treatment with ethanolic extract from Annona mucosa seeds (ESAM). Dark points inside the discs comprise honeydew drop areas excreted by Diaphorina citri adults exposed to the referred treatments, which were stained with a ninhydrin:acetone solution (1%, v/v).
Fig. 2 in Effects of cold-acclimation, pathogen infection, and varying temperatures on insecticide susceptibility, feeding, and detoxifying enzyme levels in Diaphorina citri (Hemiptera: Liviidae)
Fig. 2. Quantification of the transcription levels of 5 CYP4 genes from uninfected and Las-infected Diaphorina citri populations (A). Cq values were first normalized to the endogenous control gene actin. Standard deviations were calculated on the basis of 5 independent samples with 3 technical replicates each. Asterisks indicate statistically significant differences (P <0.05). Western blot of CYP4 microsomal proteins from 4 independent samples isolated from uninfected and Las-infected D. citri (B).
Fig. 1 in Reversal of insecticide resistance in Florida populations of Diaphorina citri (Hemiptera: Liviidae)
Fig. 1. Resistance ratios at the 50% response level using the most susceptible population as a comparison for the 3 nicotinic acetylcholine receptor agonists evaluated in this study. Abbreviations for insecticides are FLU, flupyradifurone; IMID, imidacloprid; THIA, thiamethoxam. Abbreviations for sites are LB, LaBelle; LA, Lake Alfred; SL, Port St. Lucie; WG, Winter Garden.
Fig. 2 in Mexican strains of Hirsutella isolated from Diaphorina citri (Hemiptera: Liviidae): Morphologic and molecular characterization
Fig. 2. Phylogenetic tree constructed with sequences of the 28S ribosomal gene of 14 Hirsutella species enlisted in the NCBI and 7 Mexican strains isolated from D. citri in Mexico. Percentages from bootstrap analysis that support branches in the tree are shown in the respective nodes. Scale represents the number of substitutions/100 nucleotides.
Fig. 3 in Mexican strains of Hirsutella isolated from Diaphorina citri (Hemiptera: Liviidae): Morphologic and molecular characterization
Fig. 3. Percentage of mucilaginous colonies developed from fragments of mycelial colonies of 7 Hirsutella Mexican strains afer 5 days under dark conditions. Values followed by the same letter did not differ statistically (Tukey; P = 0.05). Lines in the bars indicate SD.
Fig. 1. Diaphorina citri surveys between April 2013 and 2016 in First records of parasitoids attacking the Asian citrus psyllid in Ecuador
Fig. 1. Diaphorina citri surveys between April 2013 and 2016. Points represent monitored areas in Guayas province, Ecuador. The lower box represents where the province is located.
Figura 2 in Insecticidas de bajo impacto ambiental para el control de Diaphorina citri Kuwayama, 1908 (Hemiptera: Liviidae) en limón mexicano (Citrus aurantifolia (Christm.) Swingle)
Figura 2. Comparación de la eficiencia de los tratamientos en las diferentes fechas de muestreo. / Comparison of the efficiency of the treatments in the different sampling dates.
Data from: Genetic variation and structure of Diaphorina citri (Hemiptera:Liviidae) in populations from México
The Asian citrus psyllid, Diaphorina citri Kuwayama, is native to Asia but has recently invaded North America. Asian citrus psyllid is a significant pest of citrus crops by its direct feeding but, more importantly, as the vector of the bacterium 'Candidatus Liberibacter asiaticus', which causes Huanglongbing disease. Asian citrus psyllid was first found in México in 2001 and 2002 and, since then, has spread quickly across the country, suggesting rapid adaptation to new environments. Yet, we lack information on the genetic variation and structure that could facilitate or inhibit adaptation. Using six microsatellite markers, we analyzed genetic variation and structure among six localities in México: three in western states near the Pacific coast and two in the Yucatán Peninsula near the Gulf of México. We found low genetic diversity (no more than three alleles per locus) and intermediate differentiation between all populations. Asian citrus psyllid populations clustered into two genetic groups, but, surprisingly, these clusters were present in western populations. The first group included El Arenal, and the second group included Autlán de Navarro, Colima, and Tecomán. Interestingly, both of the Yucatán populations shared variation from the two clusters, suggesting admixture. We infer that reproductive isolation, barriers to gene flow, local selection, and the possibility of multiple invasions have influenced the current genetic structure of Asian citrus psyllid in México.
Data from: Genetic variation and structure of Diaphorina citri (Hemiptera:Liviidae) in populations from México
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Data from: Screening sticky cards as a simple method for improving efficiency of Diaphorina citri (Hemiptera: Liviidae) monitoring and reducing non-target organisms
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Transcriptional Analyses of citrus host defense response repression by feeding of Diaphorina citri transmitting Candidatus Liberibacter asiaticus at early stages of infection
GEO Series GSE154151. Citrus sinensis. 17 samples. Type: Expression profiling by high throughput sequencing.
Transcriptomic analysis of early stages of ‘Candidatus Liberibacter asiaticus’ infection in susceptible and resistant genotypes after inoculation by Diaphorina citri feeding on young shoots
GEO Series GSE277583. Murraya koenigii; Citrus sinensis; Murraya paniculata. 117 samples. Type: Expression profiling by high throughput sequencing.
Data from: Global mtDNA genetic structure and hypothesized invasion history of a major pest of citrus, Diaphorina citri (Hemiptera: Liviidae)
The Asian citrus psyllid Diaphorina citri Kuwayama, is a key pest of citrus as the vector of the bacterium causing the 'huanglongbing' disease (HLB). To assess the global mtDNA population genetic structure, and possible dispersal history of the pest, we investigated genetic variation at the COI gene collating newly collected samples with all previously published data. Our dataset consists of 356 colonies from 106 geographic sites worldwide. High haplotype diversity (H-mean = 0.702 ± 0.017), low nucleotide diversity (π-mean = 0.003), and significant positive selection (Ka/Ks = 32.92) were observed. Forty-four haplotypes (Hap) were identified, clustered into two matrilines: Both occur in southeastern and southern Asia, North and South America, and Africa; Lineages A and B also occur in eastern and western Asia, respectively. The most abundant haplotypes were Hap4 in lineage A (35.67%), and Hap9 in lineage B (41.29%). The haplotype network identified them as the ancestral haplotypes within their respective lineages. Analysis of molecular variance showed significant genetic structure (FST = 0.62, p < 0.0001) between the lineages, and population genetic analysis suggests geographic structuring. We hypothesize a southern and/or southeastern Asia origin, three dispersal routes, and parallel expansions of two lineages. The hypothesized first route involved the expansion of lineage B from southern Asia into North America via West Asia. The second, the expansion of some lineage A individuals from Southeast Asia into East Asia, and the third involved both lineages from Southeast Asia spreading westward into Africa and subsequently into South America. To test these hypotheses and gain a deeper understanding of the global history of D. citri more data-rich approaches will be necessary from the ample toolkit of next generation sequencing (NGS). However, the current paper may serve to guide such sampling and in the development of biological control programs against the global pest D. citri.
Data from: Global mtDNA genetic structure and hypothesized invasion history of a major pest of citrus, Diaphorina citri (Hemiptera: Liviidae)
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ChIP-seq experiment of transcription factor NFAT in Diaphorina citri
GEO Series GSE314527. Diaphorina citri. 2 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
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