Find research datasets worth reusing

opencc-by-4.0Mar 2022View details →

DA Diffusion Simulation for: A fluorescent nanosensor paint reveals the heterogeneity of dopamine release from neurons at individual release sites

Code developed for the revision of: Elizarova, S., Chouaib, A., Shaib, A., Mann, F., Brose, N., Kruss, S., & Daniel, J. A. (2021). A fluorescent nanosensor paint reveals the heterogeneity of dopamine release from neurons at individual release sites. BioRxiv, 2021.03.28.437019. https://doi.org/10.1101/2021.03.28.437019

opencc-zeroDec 2021View details →

Dataset for dopamine manipulated daphnia

The neurotransmitter dopamine has been shown to play an important role in modulating behavioural, morphological and life-history responses to food abundance. However, costs of expressing high dopamine levels remain poorly studied and are essential for understanding the evolution of the dopamine system. Negative maternal effects on offspring size from enhanced maternal dopamine levels have previously been documented in Daphnia. Here, we tested whether this translates into fitness costs in terms of lower starvation resistance in offspring. We exposed Daphnia magna mothers to aqueous dopamine (2.3 mg/L or 0 mg/L for the control) at two food levels (ad libitum versus 30% ad libitum) and recorded a range of maternal life history traits. The longevity of their offspring was then quantified in the absence of food. In both control and dopamine treatments, mothers that experienced restricted food ration had lower somatic growth rates and higher age at maturation. Maternal food restriction also resulted in production of larger offspring that had a superior starvation resistance, compared to ad libitum groups. However, although dopamine exposed mothers produced smaller offspring than controls at restricted food ration, these smaller offspring survived longer under starvation. Hence, maternal dopamine exposure provided an improved offspring starvation resistance.

opencc-zeroApr 2022View details →

Towards bio-inspired polymer adhesives: Activation assisted via HOBt for grafting of dopamine onto poly(acrylic acid)

The design of bio-inspired polymers has long been an area of intense study, however applications to the design of concrete admixtures for improved materials performance have been relatively unexplored. In this work we functionalized poly(acrylic acid) (PAA), a simple analogue to polycarboxylate ether admixtures in concrete, with dopamine to form a catechol-bearing polymer (PAA-g-DA). Synthetic routes utilizing hydroxybenzotriazole (HOBt) as an activating agent were examined for their ability in grafting dopamine to the PAA backbone. Previous literature using the traditional coupling reagent 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) to graft dopamine to PAA were found to be inconsistent and the sensitivity of EDC coupling reactions necessitated a search for an alternative. Additionally, HOBt allowed for greater control over percent functionalization of the backbone, is a simple, robust reaction, and showed potential for scalability. This finding also represents a novel synthetic pathway for amide bond formation between dopamine and PAA. Finally, we performed preliminary adhesion studies of our polymer on rose granite specimens and demonstrated a 56% improvement in the median adhesion strength over unfunctionalized PAA. These results demonstrate an early study on the potential of PAA-g-DA to be utilized for improving the bonds within concrete.

opencc-by-4.0Apr 2022View details →

A synaptomic analysis reveals dopamine hub synapses in the mouse striatum : Source Data

Source data for our study on dopamine hub synapses Dopamine transmission is involved in reward processing and motor control, and its impairment plays a central role in numerous neurological disorders. Despite its strong pathophysiological relevance, the molecular and structural organization of the dopaminergic synapse remains to be established. Here, we used targeted labelling and fluorescence activated sorting to purify striatal dopaminergic synaptosomes. We provide the proteome of dopaminergic synapses with 57 proteins specifically enriched. Beyond canonical markers of dopamine neurotransmission such as dopamine biosynthetic enzymes and cognate receptors, we validated 6 proteins not previously described as enriched (Cpne7, Apba1/Mint1, Cadps2, Cadm2/SynCAM 2, Stx4, Mgll). Moreover, our data reveal the adhesion of dopaminergic synapses to glutamatergic, GABAergic or cholinergic synapses in structures we named “dopamine hub synapses”. At glutamatergic synapses, pre- and postsynaptic markers are significantly increased upon association with dopamine synapses. Dopamine hub synapses may thus support local dopaminergic signalling, complementing volume transmission thought to be the major mechanism by which monoamines modulate network activity.

opencc-zeroMay 2022View details →

Dopamine activity in the tail of the striatum, DeepLabCut and MoSeq during novel object exploration

In this study, we characterized dynamics of novelty exploration using multi-point tracking (DeepLabCut) and behavioral segmentation (MoSeq). Mice were habituated in an arena, and then a object was placed at the corner of the arena. We compared 4 groups of mice: one with presentation of a novel object (stimulus novelty), one with a presentation of a familiar object (contextual novelty), one with presentation of a novel object after ablation of dopamine neuorns that project to the tail of the striatum (TS), and one with presentation of a novel object after sham surgery. With a separate group of mice, dopamine activity in TS was recorded during novelty exploration.

opencc-zeroJun 2022View details →

Population and single dopamine neuron activity during classical conditioning

We trained naive or trained mice to associate odor cues with outcome (water, air puff or no outcome), and recorded dopamine cell body activity in the vetral tegmental area (VTA), dopamine axon activity in the ventral striatum (VS) or dopamine release in VS with optic fiber fluorometry (photometry). In different set of mice, single dopamine neuron activiy was recorded with 2-photon microscope. In some of these mice, we reversed odor-outcome contingency so that an odor that was associated with no outcome or air puff became associated with water reward. Licking pattern was also recorded.

opencc-zeroApr 2024View details →

Data from: Mesostriatal dopamine is sensitive to changes in specific cue-reward contingencies

Learning causal relationships relies on understanding how often one event precedes another. To gain an understanding of how dopamine neuron activity and neurotransmitter release change when a retrospective relationship is degraded for a specific pair of events, we used outcome-selective Pavlovian contingency degradation in rats. Two cues were paired with distinct food rewards, one of which was also delivered in the absence of either cue. Conditioned responding was attenuated for the cue-reward contingency that was degraded. Dopamine neuron activity in the midbrain and dopamine release in the ventral striatum in response to the cue and subsequent reward were attenuated during degraded versus non-degraded trials, and contingency degradation also abolished the trial-by-trial history dependence of dopamine responses at the time of trial outcome. This profile of changes in cue- and reward-evoked responding is not easily explained by a standard reinforcement learning model. An alternative model based on learning causal relationships was better able to capture evoked dopamine responses during contingency degradation, as well as conditioned behavior following optogenetic manipulations of dopamine during noncontingent rewards. Our results suggest that mesostriatal dopamine encodes the contingencies between meaningful events during learning.

opencc-by-4.0May 2024View details →

Dataset associated with "Constraints on the subsecond modulation of striatal dynamics by physiological dopamine signaling"

This repository contains behavioral data, dLight photometry measurements, smoothed spiking data from recorded striatal neurons, and miniscope recordings. Text files with further explanation are provided to assist with replication of the main analyses. Matlab code to perform key analyses can be found in the accompanying GitHub repository (https://github.com/sotmasman/Dopamine-constraints)

opencc-by-4.0May 2024View details →

Data for Huang, Luo, et al., Dopamine-mediated interactions between short- and long-term memory dynamics, Nature, 2024

Zip files contain voltage imaging traces and on-ball behavioral data from the five main figures in Huang, Luo, et al., Dopamine-mediated interactions between short- and long-term memory dynamics, Nature, 2024

opencc-by-4.0Feb 2022View details →

Dopamine signaling in the dorsomedial striatum promotes compulsive behavior -- Western Blot Supplement

Compulsive behavior is a defining feature of disorders such as substance use disorders. Current evidence suggests that corticostriatal circuits control the expression of established compulsions, but little is known about the mechanisms regulating the development of compulsions. We hypothesized that dopamine, a critical modulator of striatal synaptic plasticity, could control alterations in corticostriatal circuits leading to the development of compulsions (defined here as continued reward seeking in the face of punishment). We used dual-site fiber photometry to measure dopamine axon activity in the dorsomedial striatum (DMS) and the dorsolateral striatum (DLS) as compulsions emerged. Individual variability in the speed with which compulsions emerged was predicted by DMS dopamine axon activity. Amplifying this dopamine signal accelerated animals’ transitions to compulsion, whereas inhibition delayed it. In contrast, amplifying DLS dopamine signaling had no effect on the emergence of compulsions. These results establish DMS dopamine signaling as a key controller of the development of compulsive reward seeking.

Data for "Transient nicotine exposure in early adolescent male mice freezes their dopamine circuits in an immature state"

This repository contains data for the analysis used in the following paper: Reynolds et al. "Transient nicotine exposure in early adolescent male mice freezes their dopamine circuits in an immature state", published on BioRxiv (https://www.biorxiv.org/content/10.1101/2023.10.28.564518v1). Information about using the data files is in the "readme.rtf" file.

Cholinergic Interneuron Firing and Dopamine release across striatal subregions

Folder contains all the code and data to produce the figures for "A mismatch between striatal cholinergic pauses and dopaminergic reward prediction errors"

Transgenic A53T mice have astrocytic -synuclein aggregates in dopamine and striatal regions

Statistical analysis carried out on astrocyte quantification data derived from 6 month transgenic A53T PD mice.

Transgenic A53T mice have astrocytic a-synuclein aggregates in dopamine and striatal regions

Statistical analysis carried out on astrocyte quantification data derived from 6 month transgenic A53T PD mice.

opencc-by-4.0Jul 2024View details →

MERFISH Dataset of Mouse Dopamine Neurons_03Jul2024

Cell_by_gene and Cell_metadata files from MERFISH runs of mouse Midbrain spatial transcriptomics. Datasets are setup to be analyzed using Seurat v5.

opencc-by-4.0Aug 2023View details →

Characterizing the diversity of enteric neurons using Dopamine Transporter (DAT)-Cre reporter mice

The enteric nervous system (ENS) comprises a complex network of neurons whereby a subset appears to be dopaminergic, although the characteristics, roles, and implications in disease are less understood. Most investigations relating to enteric dopamine (DA) neurons rely on immunoreactivity to tyrosine hydroxylase (TH) - a rate-limiting enzyme in the production of DA. However, TH immunoreactivity is likely to provide an incomplete picture given previous work has showed that some DA neurons contain little if any TH and its levels tend to be decreased in response to cellular stress. The study herein provides a comprehensive characterization of DA neurons in the gut using a well-accepted reporter mouse line, expressing a fluorescent protein under control of the DA transporter (DAT) promoter. Our findings confirm a unique localization of DA neurons in the gut and unveil the discrete subtypes of DA neurons in this organ, which we characterized using both immunofluorescence and single-cell transcriptomics, validated by in situhybridization. We observed distinct subtypes of DAT neurons expressing co-transmitters and modulators across both plexuses; some of them likely co-releasing acetylcholine, and a smaller population likely releasing nitric oxide; while others were positive for a slew of canonical DA markers. Interestingly, we uncovered a seemingly novel population of DA neurons unique to the ENS which were ChAT/DAT-positive neurons and characterised by the expression of Th, Grp,Calcb and Sst. Given the clear heterogeneity of DAergic gut neurons, further investigation is warranted to define their functional signatures and discover any inherent vulnerabilities in disease.

opencc-zeroAug 2023View details →

Data from: Developmental exposure to the Parkinson's disease-associated organochlorine pesticide dieldrin alters dopamine neurotransmission in α-synuclein pre-formed fibril (PFF)-injected mice

Parkinson's disease (PD) is the fastest-growing neurological diseases worldwide, with increases outpacing aging and most rapid in recently industrialized areas, suggesting the role of environmental factors. Together, epidemiological studies, post-mortem analysis and mechanistic studies suggest that exposure to persistent organic pollutants, including the organochlorine pesticide dieldrin, increases PD risk. In mouse models, developmental dieldrin exposure causes male-specific exacerbation of neuronal susceptibility to MPTP and synucleinopathy. Specifically, developmental dieldrin exposure induces male-specific exacerbation of toxicity in the α-synuclein (α-syn) pre-formed fibril (PFF) model with increased deficits in striatal dopamine (DA) turnover and motor deficits on the challenging beam. Here, we hypothesized that alterations in DA handling contribute to the observed changes and assessed vesicular monoamine transporter 2 (VMAT2) function and DA release in this dieldrin/PFF two-hit model. Female C57BL/6 mice were exposed to 0.3 mg/kg dieldrin or vehicle every 3 days by feeding, starting at 8 weeks of age by ingestion and continuing throughout breeding, gestation, and lactation. Male offspring from independent litters underwent unilateral, intrastriatal injections of α-syn PFFs at 12 weeks of age and vesicular 3H-DA uptake assays and fast-scan cyclic voltammetry (FSCV) were performed at 4 months post-PFF injection. We observed a dieldrin-induced increase in DA release in striatal slices in PFF-injected animals, but no change in VMAT2 activity. These results suggest that developmental dieldrin exposure increases a compensatory response to synucleinopathy-triggered striatal DA loss and supports our hypothesis that alterations in DA handling may underly the observed exacerbation of PFF-induced deficits in motor behavior and DA turnover.