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81 results for “ELISA”
Role of Anti-mouse PLA2R1 ELISA in Membranous Nephropathy
ClinicalTrials.gov study NCT02199145. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Serum Soluble Endoglin and Matrix Metalloproteinase 14 With Elisa Method in Early/Late-onset Preeclampsia
ClinicalTrials.gov study NCT03932877. IPD Sharing: UNDECIDED. Countries: 1. Publications: 4.
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Buccal Screening Evaluation Using an RT-PCR Assay and a Rapid ELISA Test Among Symptomatic and Asymptomatic Patients
ClinicalTrials.gov study NCT05074745. IPD Sharing: NO. Countries: 1. Publications: 1.
Efficacy Study of a Urine DEK ELISA for Diagnosis of Bladder Cancer
ClinicalTrials.gov study NCT01563796. IPD Sharing: Not stated. Countries: 1. Publications: 1.
The Specificity, Sensitivity and Clinical Correlation of CBA, RIPA and ELISA in Detecting AChR and MuSK IgG of Myasthenia Gravis
ClinicalTrials.gov study NCT05219097. IPD Sharing: NO. Countries: 1. Publications: 1.
Dataset: Elisa cohort study on SARS-CoV-2 transmission
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Gene expression and ELISA data
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Data from: Immunoglobulin detection in wild birds: effectiveness of three secondary anti-avian IgY antibodies in direct ELISAs in 41 avian species
1.Immunological reagents for wild, non-model species are limited or often non-existent for many species. 2. In this study, we compare the reactivity of a new anti-passerine IgY secondary antibody with existing secondary antibodies developed for use with birds. Samples from 41 species from the following six avian orders were analysed: Anseriformes (1 family, 1 species), Columbiformes (1 family, 2 species), Galliformes (1 family, 1 species), Passeriformes (16 families, 34 species), Piciformes (1 family, 2 species) and Suliformes (1 family, 1 species). Direct ELISAs were performed to detect total IgY using goat anti-passerine IgY, goat anti-chicken IgY or goat anti-bird IgY secondary antibodies. 3.The anti-passerine antibody exhibited significantly higher IgY reactivity compared to the anti-chicken and/or anti-bird antibodies in 80% of the passerine families tested. Birds in the order Piciformes (woodpeckers) and order Suliformes (cormorants) were poorly detected by all three secondary antibodies. A comparison of serum and plasma IgY levels was made within the same individuals for two passerine species (house finch and white-crowned sparrow), and serum exhibited significantly more IgY than the plasma for all three secondary antibodies. This result indicates that serum may be preferred to plasma when measuring total antibody levels in blood. 4.This study indicates that the anti-passerine IgY secondary antibody can effectively be used in immunological assays to detect passerine IgY for species in most passerine families and is preferred over anti-chicken and anti-bird secondary antibodies for the majority of passerine species. This anti-passerine antibody will allow for more accurate detection and quantification of IgY in more wild bird species than was possible with previously available secondary antibodies.
Data from: Interpreting ELISA analyses from wild animal samples: some recurrent issues and solutions
1. Many studies in disease and immunological ecology rely on the use of assays that quantify the amount of specific antibodies (immunoglobulin) in samples. Enzyme-Linked Immuno Sorbent Assays (ELISAs) are increasingly used in ecology due to their availability for a broad array of antigens and the limited amount of sampling material they require. Two recurrent methodological issues are nevertheless faced by researchers: (i) the limited availability of immunological assays and reagents developed for non-model species, and (ii) the statistical determination of the cut-off threshold used to distinguish individual samples that are likely to have or not to have antibodies against a specific antigen. 2. Here, we outline two solutions to deal with these issues. First, we show that implementing two assays with differing detection methods can help validate the use of reagents, such as antibodies, in species different from their intended target. We illustrate this by comparing the quantification of specific vaccinal antibodies against Newcastle Disease Virus (NDV) using two ELISA approaches in four seabird species (Cory's shearwater, European shag, European storm petrel, and Southern rockhopper penguin). 3. Second, we provide a simple way to determine from the distribution of ELISA values whether the assayed samples are likely to be made of a single group of individuals (likely negative) or of two groups of individuals (negative and positive). We illustrate the use of this approach with two independent datasets: NDV antibody levels following vaccination and anti-Borrelia antibody levels following natural exposure. 4. The practical implementation of these methodological approaches could provide a way to efficiently apply ELISAs and other immune-based assays to address questions in the growing fields of ecological immunology and disease ecology.
20240215_Elisa_Fractions
<p>20240215_Elisa_Fractions</p>
20240124_Elisa_Fractionation_Control
<p>20240124_Elisa_Fractionation_Control, Prefractionation</p>
20240221_Elisa_Spots_Frac_17_18
<p>20240221_Elisa_Spots_Frac_17_18</p>
ELISA
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ELISA
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ELISA TE
<p>Database of 544 patients screened for IgG against 4 food-borne zoonotic parasites</p>
Technical evaluation of recombinant-Ag-based InBios ELISA for the diagnosis of S Stercoralis
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Additional ELISA data for the manuscript "Restrained expansion of the recall germinal center response as biomarker of protection for influenza vaccination in mice"
<p>This repository contains the source ELISA data for supplementory figure 4 of the manuscript "Restrained expansion of the recall germinal center response as biomarker of protection for influenza vaccination in mice" currently under review by PLOS ONE.</p> <p>Files include Raw OD's per plate and reported values.</p> <p>Purpose:<br> These ELISA's were performed to assess differences in hemagglutinin (HA) specific IgG responses in sera of mice immunized with one of three univeral flu vaccine regimens conferring different levels of protection against lethal H1N1 influenza challenge.</p> <p>Method:<br> Naïve 8-weeks old mice were immunized three times, at days 0, 21 and 42 with different immunogens. One cohort of animals was vaccinated received a largely protective vaccine containing a high dose (30 µg) of a trimeric full-length H1 HA antigen (FL H1#2316) that provides heterologous protection against H1N1 A/Netherlands/602/2009 while a second cohort received a minimally protective vaccine containing a high dose (30 µg) of an HA stem-based monomeric antigen (UFV#4157) derived from FL H1#2316. To control for differences in immune responses related to the dose rather than intrinsically protective properties of the used immunization antigen, a lower but partiallly protective protective dose (0.3 µg) of FL H1#2316 was used for immunizations of a third cohort, while as a negative control for survival a fourth cohort was immunized with PBS only. All the immunized proteins are based on the HA of H1N1 A/Brisbane/59/07. All immunizations, including mock-immunizations with PBS, were adjuvanted with alum. 8 animals per cohort were sacrificed at 8 different timepoints (days 4, 7, 12, 19, 25, 28, 46 and 49) to obtain serum and lymphnodes for analaysis To assess the protective efficacy of the administered vaccine regimens, 10 animals per immunized cohort were challenged intranasally with a lethal dose of 25xLD50 H1N1 A/Netherlands/602/2009. 2 days prior to the challenge, on day 68, we also took a blood sample to check the pre-challenge titers of each animal.</p> <p>On the serum isolated on day 4, 7, 12, 19, 25, 28, 46 49 andd 68, we performed two ELISA experiments. In one ELISA we coated the plates with the recombinant HA of A/Brisbane/59/07, homologous to the used vaccine candidates, and in the other ELISA we used recombinant HA of A/California/07/09, 99.4% homologous to the challenge strain H1N1 A/Netherlands/602/2009.</p> <p>Results:<br> At the end of the three immunizations we see that the IgG titers against A/Brisbane/59/07 reach similar levels in both the 30µg and 0.3µg UFV#2316 cohorts, whearas the responses of the headless UFV#4157 are slightly lower. Because the titers against the rHA A/California/07/09 in the UFV#4157 cohort is similar to both UFV#2316 regimen we can allocate the differences observed in the A/Brisbane/59/07 ELISA to the absense or availablility of the immunodominant head domain. In conclusion we do not see any differences in antibody titers that can correlate with the challenge outcome at the end of the study:<br> 30µg UFV#2316 80% protection<br> 0.3µg UFV#2316 60% protection<br> 30µg UFV#4157: 0% protection<br> PBS: 0% protection</p>
Figure 3 in Identification of blood meals in field collected Culex pipiens, Anopheles sacharovi and Culex tritaeniorhynchus (Diptera: Culicidae) using the ELISA method
Figure 3. The percentage distribution of single and multiple host meal choices of A) An. sacharovi, B) Cx. pipiens, C) Cx. tritaeniorhynchus between humans and cows in both the Aegean and Mediterranean regions.
ELISA in Relapsed/Refractory MM
ClinicalTrials.gov study NCT06832865. IPD Sharing: YES. Countries: 1. Publications: 0.
Acceptability/Feasibility of HIV Testing by ELISA or Rapid Test to Adult Patients in Paris, France
ClinicalTrials.gov study NCT02574208. IPD Sharing: NO. Countries: 0. Publications: 1.
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Allen Brain Atlas
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.