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339 results for “In vitro models”
Evaluation of in vitro rat and human airway epithelial models for acute inhalation toxicity testing
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Ions release from hydroxyapatite and substituted hydroxyapatites in different immersion liquids: In vitro experiments and theoretical modelling study
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Benzalkonium Chloride Disinfectants Induce Apoptosis, Inhibit Proliferation, and Activate the Integrated Stress Response in a 3-D in Vitro Model of Neurodevelopment
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Raw in vitro screening data and R scripts for: A Bayesian method for population-wide cardiotoxicity hazard and risk characterization using an in vitro human model
<p>Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes are an established model for testing potential chemical hazards. Inter-individual variability in toxicodynamic sensitivity has also been demonstrated <i>in vitro</i>; however, quantitative characterization of the population-wide variability has not been fully explored. We sought to develop a method to address this gap by combining a population-based iPSC-derived cardiomyocyte model with Bayesian concentration-response modeling. A total of 136 compounds, including 44 pharmaceuticals and 82 environmental chemicals, were tested in iPSC-derived cardiomyocytes from 43 non-diseased humans. Hierarchical Bayesian population concentration-response modeling was conducted for five phenotypes reflecting cardiomyocyte function or viability. Toxicodynamic variability was quantified through the derivation of chemical- and phenotype-specific variability factors (TDVF). Toxicokinetic modeling was used for probabilistic <i>in vitro</i>-to-<i>in vivo </i>extrapolation in order to derive population-wide margins of safety (MOS) for pharmaceuticals and margins of exposure (MOE) for environmental chemicals. Pharmaceuticals were found to be active across all phenotypes. Over half of tested environmental chemicals showed activity in at least one phenotype, most commonly positive chronotropy. TDVF estimates for the functional phenotypes were greater than those for cell viability, usually exceeding the generally-assumed default of ~3. Population variability-based MOS for pharmaceuticals were correctly predicted to be relatively narrow, between 10-100; however, MOE for environmental chemicals, based on population exposure estimates, generally exceeded 1000, suggesting they pose little risk at general population exposures even to sensitive sub populations. This study represents a first of its kind human <i>in vitro</i> model that can be used to characterize toxicodynamic population variability in cardiotoxic risk.</p>
Raw data for the article: Two Sides of The Same Coin: Normal and Tumoral Stem Cells, The Relevance of In Vitro Models and Therapeutic Approaches: The Experience with Zika Virus in Nervous System Development and Glioblastoma Treatment
<p>Neural stem cells (NSCs) were described for the first time more than two decades ago for their ability to differentiate into all neural cell lineages. The isolation of NSCs from adults and embryos was carried out by various laboratories and in different species, from mice to humans. Similarly, no more than two decades ago, cancer stem cells were described. Cancer stem cells, previously identified in hematological malignancies, have now been isolated from several solid tumors (breast, brain, and gastrointestinal compartment). Though the origin of these cells is still unknown, there is a wide consensus about their role in tumor onset, propagation and, in particular, resistance to treatments. Normal and neoplastic neural stem cells share common characteristics, and can thus be considered as two sides of the same coin. This is particularly true in the case of the Zika virus (ZIKV), which has been described as an inhibitor of neural development by specifically targeting NSCs. This understanding prompted us and other groups to evaluate ZIKV action in glioblastoma stem cells (GSCs). The results indicate an oncolytic activity of this virus vs. GSCs, opening potentially new possibilities in glioblastoma treatment.</p>
in vitro analysis of a competitive inhibition model for T7 RNA polymerase biosensors
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Caco-2 in vitro model of human gastrointestinal tract for studying the absorption of titanium dioxide and silver nanoparticles from seafood
<p><a href="https://www.sciencedirect.com/topics/chemistry/titanium-dioxide-nanoparticle">Titanium dioxide nanoparticles</a> (TiO<sub>2</sub> NPs) are widely used in industry as a white pigment (paints, paper industry and toothpastes), photocatalysts (environmental decontamination and photovoltaic cells), inorganic UV filter (sunscreens and personal care products) and as a <a href="https://www.sciencedirect.com/topics/chemistry/food-additive">food additive</a> (E171) and antimicrobial food packaging material. <a href="https://www.sciencedirect.com/topics/chemistry/silver-nanoparticle">Silver nanoparticles</a> (Ag NPs) are used in photonics, microelectronics, catalysis and medicine due to their catalytic activity, magnetic and optical polarizability, electrical and <a href="https://www.sciencedirect.com/topics/chemistry/thermal-conductivity">thermal conductivities</a> and enhanced Raman scattering. They also have antibacterial, antifungal and <a href="https://www.sciencedirect.com/topics/chemistry/antiviral-activity">antiviral activities</a>, as well as anti-inflammatory potential. The huge increase in the use of nano-based products, mainly metallic <a href="https://www.sciencedirect.com/topics/chemistry/nanoparticle">NPs</a>, implies the presence of <a href="https://www.sciencedirect.com/topics/chemistry/nanomaterial">nanomaterials</a> in the environment, and hence, the unintentional human ingestion through water or foods (gastrointestinal tract is the main pathway of NPs intake in humans).</p> <p>The presence of TiO<sub>2</sub> NPs and Ag NPs in seafood samples was firstly established using an ultrasound assisted <a href="https://www.sciencedirect.com/topics/chemistry/enzymatic-hydrolysis">enzymatic hydrolysis</a> procedure and sp-ICP-MS analysis. Several clams, cockles, mussels, razor clams, oysters and variegated scallops, which contain TiO<sub>2</sub> NPs and Ag NPs, were subjected to an <em>in vitro</em> digestion process simulating human gastrointestinal digestion in the stomach and in the small and large intestine to determine the bioaccessibility of these NPs. Caco-2 cells were selected as model of human intestinal epithelium for transport studies because of the development of membrane transporters that are responsible for the uptake of chemicals. Parameters as transepithelial electrical resistance (TEER) and permeability of Lucifer Yellow were studied for establishing cell monolayer integrity. TiO<sub>2</sub> NPs and Ag NPs transport as well as total Ti and Ag concentrations passing through the gastrointestinal epithelial barrier model (0–2 h) were assessed by sp-ICP-MS and ICP-MS in several molluscs.</p>
Raw Data for the article: Human Amnion-Derived Mesenchymal Stromal/Stem Cells Pre-Conditioning Inhibits Inflammation and Apoptosis of Immune and Parenchymal Cells in an In Vitro Model of Liver Ischemia/Reperfusion
<p>Ischemia/reperfusion injury (IRI) represents one of the leading causes of primary non-function acute liver transplantation failure. IRI, generated by an interruption of organ blood flow and the subsequent restoration upon transplant, i.e., reperfusion, generates the activation of an inflammatory cascade from the resident Kupffer cells, leading first to neutrophils recruitment and second to apoptosis of the parenchyma. Recently, human mesenchymal stromal/stem cells (hMSCs) and derivatives have been implemented for reducing the damage induced by IRI. Interestingly, sparse data in the literature have described the use of human amnion-derived MSCs (hAMSCs) and, more importantly, no evidence regarding hMSCs priming on liver IRI have been described yet. Thus, our study focused on the definition of an in vitro model of liver IRI to test the effect of primed hAMSCs to reduce IRI damage on immune and hepatic cells. We found that the IFNγ pre-treatment and 3D culture of hAMSCs strongly reduced inflammation induced by M1-differentiated macrophages. Furthermore, primed hAMSCs significantly inhibited parenchymal apoptosis at early timepoints of reperfusion by blocking the activation of caspase 3/7. All together, these data demonstrate that hAMSCs priming significantly overcomes IRI effects in vitro by engaging the possibility of defining the molecular pathways involved in this process.</p>
Development of an in vitro model of the gut microbiota enriched in mucus-adhering bacteria
<p>16S rRNA gene sequencing data in FASTQ format</p>
Raw in vitro screening data and R scripts for: A Bayesian method for population-wide cardiotoxicity hazard and risk characterization using an in vitro human model
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Data from: Bacterial competition and quorum-sensing signalling shapes the eco-evolutionary outcomes of model in vitro phage therapy
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Efficient generation of human CA3 neurons and modeling hippocampal neuronal connectivity in vitro (single cells)
GEO Series GSE111978. Homo sapiens. 507 samples. Type: Expression profiling by high throughput sequencing.
Role of human dural fibroblasts in the angiogenic responses of human endothelial cells: an in vitro dural model for EDAS
GEO Series GSE224999. Homo sapiens. 2 samples. Type: Expression profiling by high throughput sequencing.
Cancer therapy induced effects on lung organoids as suitable in vitro model
GEO Series GSE275539. Homo sapiens. 25 samples. Type: Expression profiling by high throughput sequencing.
In vitro neuronal RNA-sequencing, in vivo cortical single nucleus RNA-sequencing, in vivo cortical ChIP-sequencing of H3K79me2 in models of DOT1L loss
GEO Series GSE279978. Mus musculus. 26 samples. Type: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing.
A primary cell-based in vitro model of the human small intestine reveals the induction of OLFM4 expression as host cell response during Salmonella Typhimurium infection
GEO Series GSE217976. Homo sapiens. 2 samples. Type: Expression profiling by high throughput sequencing.
Transcriptional analysis of airway neutrophils in an in vitro model of ARDS
GEO Series GSE228144. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
The Tissue-Engineered Human Psoriatic Skin Substitute: A Valuable In Vitro Model to Identify Genes with Altered Expression in Lesional Psoriasis
GEO Series GSE120464. Homo sapiens. 13 samples. Type: Expression profiling by array.
A Lysosomal In Vitro Exposure (LivE) Model to Identify Pathways Critical for Mycobacterium tuberculosis Intracellular Persistence
GEO Series GSE68337. Mycobacterium tuberculosis CDC1551. 15 samples. Type: Expression profiling by high throughput sequencing.
Transcriptome changes during Endothelial to Mesenchymal Transition (EndMT) using a TGF-b2 and IL-1b in vitro model of EndMT in HUVEC and HPAEC
GEO Series GSE118446. Homo sapiens. 18 samples. Type: Expression profiling by high throughput sequencing.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.