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opencc-by-4.0Mar 2015View details →

Fig. 1A. Fall armyworm weights for 7 in Use of benzimidazole agar plates to assess fall armyworm (Lepidoptera: Noctuidae) feeding on excised maize and sorghum leaves

Fig. 1A. Fall armyworm weights for 7 sorghum (off white) and maize (black) cultivars for Trial 1. Among the maize lines 'AB24E' was known to be susceptible and 'Mp708' and FAW1430' were known to be resistant to fall armyworm feeding. 'AN109', 'Collier', Entry 22 and 'Honey Drip' are sorghum lines. In Trial 1, fall armyworm neonate larvae were taken from the Tifon, Georgia culture. Means with the same letter are not significantly different. Error bars represent one standard error of the mean.

opencc-by-4.0Jun 2016View details →

Fig. 1 in Population variability of Spodoptera frugiperda (Lepidoptera: Noctuidae) in maize (Poales: Poaceae) associated with the use of chemical insecticides

Fig. 1. Spodoptera frugiperda populations in cultivated maize in various parts of Mexico from which larvae were collected to study molecular genetic variation.

opencc-by-4.0Jun 2016View details →

Fig. 2 in Population variability of Spodoptera frugiperda (Lepidoptera: Noctuidae) in maize (Poales: Poaceae) associated with the use of chemical insecticides

Fig. 2. Dendrogram of genetic distance among Spodoptera frugiperda populations analyzed using ISSR molecular markers.

opencc-by-4.0Jun 2016View details →

Fig. 1 in Effect of genetically modified Bt maize in an artificial diet on the survival of Cydia pomonella (Lepidoptera: Tortricidae)

Fig. 1. Probit response on log Bt concentration of Cydia pomonella larvae reared on a diet containing maize where the responses were: (A) larval mortality Y = 2.980 + 1.27(X); (B) delayed larval development, Y = 3.789 + 1.886(X) for experiment 1 and Y = 2.840 + 1.886(X) for experiment 2; (C) larvae leaving the diet, Y = 3.595 + 0.681(X); (D) total response or response A + B + C, Y = 4.133 + 2.344(X) for experiment 1 and Y = 3.592 + 2.344(X) for experiment 2. Y is the probit response and X is the log Bt concentration.

opencc-by-4.0Dec 2018View details →

F1 Maize Iso-Seq - Final & Intermediate files

=============================================================================== Variant Phasing and Haplotypic Expression from Single-molecule Sequencing in Maize =============================================================================== Maize is a diploid species with very high genetic diversity. Haplotype phasing of genetic variants in maize is important for interpretation of the genome, population genetic and functional genomic analysis of allelic activity. However, due to splicing variability and sequencing length limitation, phasing at isoform level are always very challenge. Here, we developed a tool called ‘Iso-Phase’ to phase the isoforms in hybrids and present the first isoforms phasing study in maize using inbred lines B73 and Ki11, as well as their reciprocal crosses from full-length single-molecule sequencing. Our results show that maize parental lines and hybrid lines display different splicing activity, and 6,847 genes can be phased through Iso-Phase in two reciprocal hybrids using embryo, endosperm and root tissues. We identified parental origin isoforms in maize hybrids, different novel isoforms between maize parent and hybrid lines, provides measures of haplotypic expression that increase power and accuracy in studies of allelic expression. It is the first study of phased full-length isoforms in maize, as well as in plants, which provides insights about maize and plant heterosis at allele-specific full-length transcriptional level. The approach used in this study also provide important information for many other phasing studies in different species.

opencc-by-4.0Jul 2019View details →

Moisture content and total aflatoxin content of the freshly harvested maize samples

Moisture content and total aflatoxin content of the freshly harvested maize samples.  Moisture content of the samples were determined on-site in triplicate using Superpoint handheld moisture analyzer (Supertech Agroline, Hestchaven 5, DK-5400 Bogense, Denmark; ±0.5% accuracy) following the manufacturer’s instructions. Total AF in the samples were quantified by a single step lateral flow immunoassay utilizing the developed Reveal Q+ test strip for Aflatoxin (Neogen Item 8085) read on a calibrated AccuSan Gold reader (Neogen Corporation, 620 Lesher Place, Lansing, MI 48912 USA) (Neogen item 9595) at 18-22oC

opencc-by-4.0Sep 2019View details →

Data from: Analysis of leaf microbiome composition of near-isogenic maize lines differing in broad-spectrum disease resistance

Data and code associated with the submitted manuscript "Analysis of leaf microbiome composition of near-isogenic maize lines differing in broad-spectrum disease resistance". Detailed descriptions of each file can be found in the README.txt . The raw sequence data associated with this work can be downloaded from the NCBI Sequence Read Archive, listed under BioProject #PRJNA565009.

opencc-by-4.0Jun 2022View details →

Figure 2 in A 6-year field monitoring of fall armyworm, Spodoptera frugiperda, in transgenic Bt maize in Brazil

Figure 2 Average number of fall armyworm larvae, Spodoptera frugiperda, collected in Bt hybrids, non-Bt hybrids and non-Bt hybrids sprayed with methomyl in 2015 and 2016 (period II), in Sete Lagoas and Nova Porteirinha. Average followed by the same upper-case letter between municipalities and same lower-case letter in each municipality do not differ statistically (Scott-Knott test at p ≤ 0.05).

opencc-by-4.0Jun 2022View details →

Figure 1 in A 6-year field monitoring of fall armyworm, Spodoptera frugiperda, in transgenic Bt maize in Brazil

Figure 1 Average number of fall armyworm larvae, Spodoptera frugiperda collected in Bt hybrids, non-Bt hybrids and non-Bt hybrids sprayed with methomyl from 2011 to 2014 (period I), in Sete Lagoas and Nova Porteirinha. Average followed by the same upper-case letter between municipalities and same lower-case letter in each municipality do not differ statistically (Scott-Knott test at p ≤ 0.05).

Fig. 2 in Preference of Neoseiulus californicus (Acari: Phytoseiidae) for volatiles of Bt maize induced by multiple herbivory

Fig. 2. Predatory mite Neoseiulus californicus preference for volatile compounds in two-choice olfactometer tests, comparing: conventional maize plants infested by Tetranychus urticae (C + Tu); conventional maize plants infested by Tetranychus urticae and Spodoptera frugiperda (C + Tu + Sf); Bt maize plants infested by T. urticae (Bt + Tu); Bt maize plants infested by T. urticae and S. frugiperda (Bt + Tu + Sf). Numbers in bars represent individual predatory mites that choose the indicated odor. No significant p ≥ 0.05.

Fig. 5 in Preference of Neoseiulus californicus (Acari: Phytoseiidae) for volatiles of Bt maize induced by multiple herbivory

Fig. 5. Projection to principal component analysis (PCA) based on the headspace composition of volatiles of conventional maize plants uninfested (C = o); conventional maize plants infested by Tetranychus urticae (C + Tu = •); Bt maize plants uninfested (Bt = Δ); and Bt maize plants infested by T. urticae (Bt + Tu = N), using the first two principal components (Dim) with explained variance in brackets.

Fig. 1 in Preference of Neoseiulus californicus (Acari: Phytoseiidae) for volatiles of Bt maize induced by multiple herbivory

Fig. 1. Predatory mite Neoseiulus californicus preference for volatile compounds in two-choice olfactometer tests, comparing: conventional maize plants uninfested (C); conventional maize plants infested by Tetranychus urticae (C + Tu); Bt maize plants uninfested (Bt); and Bt maize plants infested by T. urticae (Bt + Tu); Numbers in bars represent individual predatory mites that choose the indicated odor. No significant p ≥ 0.05.

Fig. 3 in Preference of Neoseiulus californicus (Acari: Phytoseiidae) for volatiles of Bt maize induced by multiple herbivory

Fig. 3. Chromatograms of volatile compounds extracted from leaves of conventional maize plants uninfested (C); conventional maize plants infested by Tetranychus urticae (C + Tu); Bt maize plants uninfested (Bt); and Bt maize plants infested by T. urticae (Bt + Tu). The compounds observed in analysis were 1 = (E)-Hex-2-enal; 2 = heptanal; 3 = (E)-Oct-2-enal; 4 = α-pineno; 5 = β-cisocimeno; 6 = β-Ciclocitral; 7 = 1-metil-6-(3-metilbuta-1,3-dienil)-7-oxabiciclo[4,1,0]heptano; 8 = no identificated = C8; 9 = no identificated = C9; 10 = β-ionona; 11 = Ciclosativena; 12 = (E)-7-tetradecen-1-ol; 13 = no identificated = C13; 14 = Linolenic acid ethyl ester; 15 = no identificated = C15.

Fig. 4 in Preference of Neoseiulus californicus (Acari: Phytoseiidae) for volatiles of Bt maize induced by multiple herbivory

Fig. 4. Chromatograms of volatile compounds extracted from leaves of conventional maize plants infested by Tetranychus urticae (C + Tu); conventional maize plants infested by Tetranychus urticae and Spodoptera frugiperda (C + Tu + Sf = +); Bt maize plants infested by T. urticae (Bt + Tu); Bt maize plants infested by T. urticae and S. frugiperda (Bt + Tu + Sf). The compounds observed in analysis were 1 = (E)-Hex-2-enal; 2 = heptanal; 3 = (E)-Oct-2-enal; 4 = ˛-pineno; 5 = ˇ-cisocimeno; 6 = ˇ-Ciclocitral; 7 = 1-metil-6- (3-metilbuta-1,3-dienil)-7-oxabiciclo[4,1,0]heptano; 8 = ˇ-ionona; 9 = no identificated = C9; 10 = (E)-7-tetradecen-1-ol; 11 = no identificated = C11; 12 = Linolenic acid ethyl ester; 13 = no identificated = C13.

Fig. 8 in Does Bt maize cultivation affect the non-target insect community in the agro ecosystem?

Fig. 8. Estimated richness of insects in the whorl, ear and tassel of conventional (Conv.) and transgenic maize (Cry1Ab, Cry1F and combined Cry2Ab2 and Cry1A105 proteins) from Iguatama county, MG. Bars represent 95% confidence interval.

Fig. 15 in Does Bt maize cultivation affect the non-target insect community in the agro ecosystem?

Fig. 15. Estimated diversity of secondary pests (S.P.) and natural enemies (N.E.) in tassels of conventional and transgenic maize for Cry1Ab, Cry1F and combined Cry2Ab2 and Cry1A105 proteins (A) and estimated richness in conventional maize and Bt maize (B), in different counties in Minas Gerais.Bars represent 95% confidence interval.

Fig. 4 in Does Bt maize cultivation affect the non-target insect community in the agro ecosystem?

Fig. 4. Estimated richness of insects in the whorl, ear and tassel of conventional (Conv.) and transgenic maize (Cry1Ab and Cry1F proteins) from Varjão de Minas county. Bars represent 95% confidence interval.

Fig. 16 in Does Bt maize cultivation affect the non-target insect community in the agro ecosystem?

Fig. 16. Relationship between estimated richness of secondary pests and estimated richness of natural enemies in the studied cornfields.

Fig. 11 in Does Bt maize cultivation affect the non-target insect community in the agro ecosystem?

Fig. 11. Estimated diversity secondary pests (S.P.) and natural enemies (N.E.) in conventional and transgenic maize whorls for Cry1Ab, Cry1F and combined Cry2Ab2 and Cry1A105 proteins (A) and estimated diversity in conventional maize and Bt maize (B) in different counties in Minas Gerais. Bars represent 95% confidence interval.

Fig. 9 in Does Bt maize cultivation affect the non-target insect community in the agro ecosystem?

Fig. 9. Estimated richness of insects in the whorl and tassel of conventional (Conv) and transgenic maize (Cry1Ab and Cry1F proteins) from Matozinhos county, MG. Bars represent 95% confidence interval.