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56 results for “Oligohymenophorea”
Figure 4 in The search finds an end: the morphologically chimeric hysterocinetids belong to the subclass Hymenostomatia (Ciliophora: Oligohymenophorea)
Figure 4. Hysterocineta bellerophon in vivo. A, D, less-side overviews, showing the variability of body shape and size, as well as of the nuclear apparatus. B, detail of the anterior body region, showing the dense somatic ciliature and the unciliated sucker. C, detail of the vacuolized oral area. E, detail of the posterior body region, showing the oral ciliature and the vacuolized cytoplasm. CV, contractile vacuole; FV, food vacuoles; IF, infundibulum; MA, macronucleus; OC, oral cilia; S, sucker; SC, somatic cilia. Scale bars = 20 µm (E), 30 µm (B), 50 µm (A), 100 µm (D).
Figure 2 in The search finds an end: the morphologically chimeric hysterocinetids belong to the subclass Hymenostomatia (Ciliophora: Oligohymenophorea)
Figure 2. Protoptychostomum simplex in vivo (A, B) and asser protargol impregnation (C–I). A, detail of the anterior body region, showing densely arranged basal bodies of somatic kineties and the unciliated V-shaped sucker lined by regularly spaced, oblique rows of highly refractive granules. B, detail of the posterior body region, showing the oral ciliature and the vacuolized cytoplasm. C, D, less-side overviews, showing the variability of body shape and size, as well as of the nuclear apparatus. E, F, I, details of the infundibular and peristomial ciliature, as well as of the nuclear apparatus, which is composed of an ellipsoidal macronucleus and two globular micronuclei. Arrowhead in (E, F) marks the curved anterior end of membranelles M1 and M2. G, somatic kineties are composed of narrowly spaced monokinetids. Asterisks mark irregularities in the somatic ciliary paưern. H, surface view, showing cortical granules. CP, cytopharynx; F, fibre; G, granules; M1–2, membranelle 1 and 2; MA, macronucleus; MI, micronuclei; OA, oral apparatus; OC, oral cilia; PM, paroral membrane; S, sucker; SK, somatic kineties. Scale bars = 5 µm (I), 10 µm (F, G), 15 µm (A, B), 40 µm (E), 50 µm (C, D).
Figure 1 in The search finds an end: the morphologically chimeric hysterocinetids belong to the subclass Hymenostomatia (Ciliophora: Oligohymenophorea)
Figure 1. Protoptychostomum simplex in vivo (A) and asser protargol impregnation (B–I). A, less-side view of a representative specimen. B, F–I, less-side overviews, showing the variability of body shape and size as well as of the nuclear apparatus (shaded grey) and sucker (shaded yellow). Dashed circles in (I) represent contractile vacuoles. C, oral ciliary paưern. The paroral membrane and both membranelles extend along the whole posterior body end to plunge into the infundibulum where they form a helix-like paưern. Membranelle M1 is made up of two rows of basal bodies and is not segmented. Membranelle M2 runs beside M1, is composed of only a single row of basal bodies and is not segmented. Arrowhead in (C) marks the curved anterior end of membranelles M1 and M2. D, E, ciliary paưern of the less (D) and the right (E) side. CP, cytopharynx; CV, contractile vacuoles; M1–2, membranelle 1 and 2; MA, macronucleus; MI, micronuclei; PM, paroral membrane; S, sucker; SK, somatic kineties. Scale bars = 30 µm (I), 40 µm (A, B, D, E), 80 µm (H), 100 µm (F, G).
Figure 3 in The search finds an end: the morphologically chimeric hysterocinetids belong to the subclass Hymenostomatia (Ciliophora: Oligohymenophorea)
Figure 3. Hysterocineta bellerophon in vivo (A) and asser protargol impregnation (B–L). A, less-side view of a representative specimen. B, oral ciliary paưern. Membranelle M1 is made up of two rows of basal bodies and runs only on the peristome. Membranelle M2 is cut in two segments: (i) the distal segment M2 is built from two rows of basal bodies and runs beside M1, (ii) the proximal segment M2' is composed of three rows of basal bodies and extends beside M3. Membranelle M3 consists of two rows of basal bodies and starts at the infundibular entrance. C–F, I–L, less-side overviews, showing the variability of body shape and size as well as of the nuclear apparatus (shaded grey) and sucker (shaded yellow). Dashed circles represent contractile vacuole. G, H, the ciliary paưern of the less (G) and the right (H) side. Arrows mark a posterior secant system each on the ventral and the dorsal margin of the right body side. Abbreviations: CV, contractile vacuole; IF, infundibulum; M1–3, membranelles 1–3; MA, macronucleus; PM, paroral membrane; S, sucker; SK, somatic kineties. Scale bars = 50 µm (A, G, H), 100 µm (C, D, E, F, I, J, K, L).
Figure 6. Hysterocineta bellerophon asser protargol impregnation. A in The search finds an end: the morphologically chimeric hysterocinetids belong to the subclass Hymenostomatia (Ciliophora: Oligohymenophorea)
Figure 6. Hysterocineta bellerophon asser protargol impregnation. A, detail of the anterior body region of the specimen shown in Figure 5A, to document the organization of somatic kineties around the sucker. Skeletal fibres emerge from the anterior end of the less ciliary rows and run almost in parallel with the main body axis. B, detail of the oral ciliary paưern near the entrance to the infundibulum of the specimen shown in Figure 5C. C, D, membranelle M1 and M2 are made up of two rows of basal bodies each, run along the peristome, and terminate near the entrance to the infundibulum. Note that the posterior row of M2 is not recognizable in (C), as it is out of focus. E, F, paroral membrane extends on the opposite side of the infundibulum as M2ʹ and M3, describing 1.5 turns of a spiral. Arrowhead in (F) denotes the anterior end of M2ʹ and M3. G, somatic kineties are composed of narrowly spaced monokinetids. Asterisk marks an irregularity in the somatic ciliary paưern. H, the macronucleus is irregularly curved cylindroidal and contains innumerable small nucleoli. The small anterior blob is a preparation artefact caused by leaked macronuclear material as evidenced by the presence of nucleoli. F, skeletal fibres; M1–3, membranelles 1–3; MA, macronucleus; PM, paroral membrane; S, sucker; SK, somatic kineties. Scale bars = 3 µm (C, D), 10 µm (F, G), 15 µm (B), 20 µm (E, H), 30 µm (A).
Figure 9 in The search finds an end: the morphologically chimeric hysterocinetids belong to the subclass Hymenostomatia (Ciliophora: Oligohymenophorea)
Figure 9. Phylogenetic tree based on the nuclear 18S and 28S rRNA gene sequences, showing the phylogenetic position of hysterocinetids within the class Oligohymenophorea. The subclass Peniculia was used as an outgroup, following Gao et al. (2016). Posterior probabilities for Bayesian inference conducted in MrBayes and bootstrap values for maximum likelihood conducted in IQ-Tree were mapped onto the 50%-majority rule Bayesian consensus tree. Fully statistically supported nodes are marked with red solid circles. The scale bar denotes one substitution per ten nucleotide positions.
Figure 13 in The search finds an end: the morphologically chimeric hysterocinetids belong to the subclass Hymenostomatia (Ciliophora: Oligohymenophorea)
Figure 13. Secondary structure of the ITS2 molecule of hysterocinetids (A, B), Tetrahymena nigricans (C), Clausilocola apostropha (D), Tetrahymena nanneyi (E), and Tetrahymena foissneri (F). Arrows in (A–C) mark molecular synapomorphies of the 'paravorax' clade. Helices of the ITS2 molecule are marked by Roman numbers I–IV, the pyrimidine-pyrimidine mismatch of helix II is denoted by an orange rectangle.
Figure 12 in The search finds an end: the morphologically chimeric hysterocinetids belong to the subclass Hymenostomatia (Ciliophora: Oligohymenophorea)
Figure 12. Secondary structure of the 5.8S rRNA molecule and the first two domains of the 28S rRNA molecule of Hysterocineta bellerophon. Arrows mark molecular synapomorphies of the 'paravorax' clade. The 5.8S-28S secondary structure map of Tetrahymena thermophila (inset) is from hưp://apollo.chemistry.gatech.edu/RibosomeGallery.
FIGURE 1 in Morphology and infraciliature of two new earthworm ciliates, Hoplitophrya polymorphus sp. nov. and Anoplophrya simplex sp. nov. (Ciliophora: Oligohymenophorea: Astomatia)
FIGURE 1. The study area
Fig. 1. Phylogram constructed for 31 in Paramecium tredecaurelia: A Unique Non-Polymorphic Species of the P. aurelia spp. Complex (Oligohymenophorea, Ciliophora)
Fig. 1. Phylogram constructed for 31 strains of the P. aurelia species complex (including the 5 studied strains of P. tredecaurelia) and two strains of P. multimicronucleatum used as an outgroup. The trees were constructed on the basis of a comparison of sequences from the ITS1- 5.8S-ITS2-5'LSU rDNA fragment (A), COI (B), and CytB (C) using the Bayesian inference method. Bootstrap values for neighbor joining, maximum parsimony analysis, maximum likelihood, and posterior probabilities for Bayesian inference are shown. Bootstrap values smaller than 50% (posterior probabilities <0.50) are not shown. Dashes represent no bootstrap or posterior value at a given node. All positions containing gaps and missing data were eliminated. Phylogenetic analyses were conducted using MEGA 5.0 (NJ/MP/ML) and MrBayes 3.1.2 (BI).
Fig. 2 in Morphological Redescription and SSU rDNA-based Phylogeny of Two Freshwater Ciliates, Uronema nigricans and Lembadion lucens (Ciliophora, Oligohymenophorea), with Discussion on the Taxonomic Status of Uronemita sinensis
Fig. 2. Uronema nigricans and Lembadion lucens in vivo (A, B) and after protargol (C–F) staining. (A, B) Right ventrolateral view (A) and ventral view (B) of a representative individual of Uronema nigricans and Lembadion lucens, respectively, arrows point to contractile vacuole. (C–F) Ventral (C, D) and dorsal (E, F) views of representative individuals of Uronema nigricans and Lembadion lucens, respec- tively, to show the ciliature and nuclear apparatus. AM, adoral membranelle; CC, basal body of caudal cilia; M1–3, membranelles 1–3; PM, paroral membrane; Sc, scutica; SK1, the somatic kinety right of buccal field; SKn, the somatic kinety left of buccal field. Scale bars: 15 μm (A, C, F); 30 μm (B, D, E).
Fig. 36 in New Paramecium (Ciliophora, Oligohymenophorea) congeners shape our view on its biodiversity
Fig. 36 SSU rDNA tree topology. Phylogenetic reconstruction of the genus Parameciom based on 18S-rDNA sequences inferred by Bayesian Inference analysis. The alignment contained 49 taxa and 1640 sites including gaps. Sequences of new or cryptic species characterized within this study are shown in boldface. Sequences of other Peniculida served as outgroup. Nombers at nodes (occasionally indicated by an arrow) represent support values for the Bayesian Inference and Maximum Likelihood
Fig. 23–26 in New Paramecium (Ciliophora, Oligohymenophorea) congeners shape our view on its biodiversity
Fig. 23–26 Morphology of BEocandidatos P. germanicum^. 23 Living cell with visible food vacuoles (FV) and macronucleus (MA). DIC contrast. 24 Ventral view of silver nitrate-impregnated cell with cytoproct (C). 25 Nuclear apparatus of the Feulgen-stained cell with indications for micronuclei (small arrows). 26 Nuclear apparatus. Feulgen-stained cell, large magnification. Bars 40 μm (23), 35 μm (24), 4.5 μm (25), 6 μm (26)
Fig. 9–16 in New Paramecium (Ciliophora, Oligohymenophorea) congeners shape our view on its biodiversity
Fig. 9–16 Morphological features of P. boetschlii sp. nov. 9–12 Nuclear apparatus and buccal region of the ciliate. 9 Buccal cavity and part of macronucleus (MA) with micronucleus (MI). Trichocysts along of cortex are also visible. 10 Nuclear apparatus. 11 Buccal overture. 12 Buccal ciliature and nuclear apparatus. 13 Posterior end of the ciliate with numerous trichocysts. 14 Pores of CV. 15–16) CV dynamic. Systole (15) and diastole (16). 9, 11–16) Living cells. DIC contrast. 10 Feulgenstained nuclear apparatus. Bars 10 μm (9–13), 5 μm (14–16)
Fig. 1–8 in New Paramecium (Ciliophora, Oligohymenophorea) congeners shape our view on its biodiversity
Fig. 1–8 General shape and cortex construction of P. boetschlii sp. nov. 1–2 Ventral 1 and dorsal 2 views of the same cell. Ampoules of contractile vacuoles (CV) and its pores (wedge-tailed arrows in Fig. 2) as well as the macronucleus (MA) and the micronucleus (large arrow in Fig. 1) are visible. 3 Deciliated cell (found by chance) from the ventral view. Position of oral aperture (OA), anterial suture (arrowhead) and cytoproct (C) are shown. 4–8 Impregnated cells: ventral 4 and dorsal 5 side of different cells and its details; quadrulus (Q), cytoproct (C) and pores of contractile vacuole (PCV). 6 Buccal overture with buccal ciliature: endoral membrane (EM) and quadrulus (Q). 7 Two pores of the same contractile vacuole. 8 Cytoproct region. 1–3 Living cells, DIC contrast. 4–8 Silver nitrate impregnation. Bars 50 μm (1–3), 25 μm (4, 5), 10 μm (6, 8), 5 μm (7)
Figure 5. Hysterocineta bellerophon asser protargol impregnation. A, B in The search finds an end: the morphologically chimeric hysterocinetids belong to the subclass Hymenostomatia (Ciliophora: Oligohymenophorea)
Figure 5. Hysterocineta bellerophon asser protargol impregnation. A, B, details of the anterior body region, showing the less (A) and the right (B) side ciliary paưern. Arrow in (A) marks the subapical secant system below the dorsal sucker arm. C, D, oral ciliary paưern. E, less-side overviews showing the general body organization. IF, infundibulum; M1–3, membranelles 1–3; MA, macronucleus; OA, oral apparatus; PM, paroral membrane; S, sucker; SK, somatic kineties. Scale bars = 20 µm (C, D), 50 µm (A, B, E).
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