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Figure 11 in Cytosystematics of the Simulium tuberosum group (Diptera: Simuliidae) in Thailand

Figure 11. IIIL arm of Simulium tani A. Sections are numbered according to the Simulium subgeneric IIIL standard of Adler & Kachvorian (2001) and reflect the presence of five fixed inversions: IIIL-1, IIIL-2, IIIL-3, IIIL-5 and IIIL-6. Fixed inversions are underlined on the map; floating inversions are not. C = centromere, CS = cup and saucer marker, NO = nucleolar organizer. Arrows indicate breakpoints of the IIIL-5 inversion.

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Figure 9 in Cytosystematics of the Simulium tuberosum group (Diptera: Simuliidae) in Thailand

Figure 9. IIIL arm of Simulium doipuiense A. Sections are numbered according to the Simulium subgeneric IIIL standard of Adler & Kachvorian (2001) and reflect the presence of three fixed inversions: IIIL-1, IIIL-11, and IIIL-13. Fixed inversions are underlined on the map; floating inversions are not. Breakpoints are indicated for inversions IIIL-8 of S. rufibasis and IIIL-14 of Unknown species 1. Inversion IIIL-4 is linked to the X chromosome in S. doipuiense B. C = centromere, CS = cup and saucer marker, NO = nucleolar organizer.

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Figure 7 in Cytosystematics of the Simulium tuberosum group (Diptera: Simuliidae) in Thailand

Figure 7. IIL arm of Simulium rufibasis, showing the standard sequence of the Thai tuberosum group. Floating inversions are indicated by brackets. Arrow indicates one breakpoint of pericentric inversion IIP-1. C = centromere, PB = parababiani marker.

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Figure 10 in The Simulium vernum group (Diptera: Simuliidae) in Europe: multiple character sets for assessing species status

Figure 10. Neighbour-joining analysis of the Kimura two-parameter (K2P) distances of mitochondrial cytochrome c oxidase I (COI) 800-bp sequences from Simulium crenobium (Knoz, 1961), Simulium juxtacrenobium Bass & Brockhouse, 1990, and Simulium vernum s.s. Macquart, 1826 in relation to Simulium costatum Friederichs, 1920; AU, Austria; EN, England; FN, Finland; SW, Sweden.

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Figure 11 in The Simulium vernum group (Diptera: Simuliidae) in Europe: multiple character sets for assessing species status

Figure 11. Neighbour-joining analysis of the Kimura two-parameter (K2P) distances of combined barcode cytochrome c oxidase I (COI) + COI 800-bp sequences from Simulium crenobium (Knoz, 1961), Simulium juxtacrenobium Bass & Brockhouse, 1990, and Simulium vernum s.s. Macquart, 1826 in relation to Simulium costatum Friederichs, 1920; AU, Austria; EN, England; FN, Finland; SW, Sweden.

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Figure 4 in The Simulium vernum group (Diptera: Simuliidae) in Europe: multiple character sets for assessing species status

Figure 4. Larval head pigmentation viewed (A) laterally and (B, C) dorsally, and (D) antenna, (E) hypostoma, and (F–J) postgenal cleft of Simulium juxtacrenobium Bass & Brockhouse, 1990; examples from Finland show variation of apotome pigmentation (B, C), and variation in postgenal cleft shape and associated pigmentation (F–J). Scale bars: 0.1 mm.

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Figure 7. Two morphologically different B in The Simulium vernum group (Diptera: Simuliidae) in Europe: multiple character sets for assessing species status

Figure 7. Two morphologically different B chromosomes of Simulium juxtacrenobium Bass & Brockhouse, 1990 (female larva from Sweden, site 15).

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Figure 2 in The Simulium vernum group (Diptera: Simuliidae) in Europe: multiple character sets for assessing species status

Figure 2. Base of a typical gill of a Finnish pupa of Simulium juxtacrenobium Bass & Brockhouse, 1990.

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Figure 3 in The Simulium vernum group (Diptera: Simuliidae) in Europe: multiple character sets for assessing species status

Figure 3. Cephalic cuticle of pupae, showing the location of the cross-sections for counting microtubercles (100¥), and sections of the surface sculpture (200¥) of (A) S. juxtacrenobium Bass & Brock., (B) Simulium crenobium (Knoz, 1961), and variations (C, D) of Simulium vernum s.s. Macquart, 1826 across the right-hand side of the middle transect. Scale bars: 0.1 mm.

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Figure 1 in The Simulium vernum group (Diptera: Simuliidae) in Europe: multiple character sets for assessing species status

Figure 1. Ventral plate (vp; ventral view), left gonostylus (gl; posteromedial view), and gonostyli (gst; posterior view) of (A) Simulium crenobium (Knoz, 1961), (B) Simulium juxtacrenobium Bass & Brockhouse, 1990, and (C) Simulium vernum s.s. Macquart, 1826. Length and width measurements of basal arms (al, aw) and body (bl, bw) indicated in (b). Scale bar: 0.1 mm.

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Figs 128–149 in Simulium (Trichodagmia) (Diptera, Simuliidae) phylogeny revisited: the Neotropical and Afrotropical connection

Figs 128–149. Larva (last instar). Postgenal cleft. 128–131. Simulium (Anasolen) Enderlein, 1930. 128. ambositrae Grenier & Grjebine, 1959. 129. dentulosum Roubaud, 1915. 130. imerinae Roubaud, 1905. 131. nili Gibbins, 1934. — 132–135. S. (Freemanellum) Crosskey, 1969. 132. berghei Fain, 1949. 133. hirsutilateris De-Meillon, 1937. 134. debegene De-Meillon, 1934. 135. manense Elsen & Escaffre, 1976. — 136–139. S. (Hearlea) Vargas et al., 1946. 136. ayrozai Vargas, 1945. 137. canadense Hearle, 1932. 138. capricorne De León, 1945. 139. larvispinosum De León, 1948. — 140–143. S. (Hemicnetha) Enderlein, 1934. 140. bricenoi Vargas et al., 1946. 141. cristalinum Coscarón & Py-Daniel, 1989. 142. paynei Vargas, 1942. 143. rubrithorax Lutz, 1909. — 144. S. (Obuchovia) galloprovinciale Giudicelli, 1963. — 145–147. S. (Trichodagmia) Enderlein, 1934. 145. hirtipupa Lutz, 1910. 146. lahillei (Paterson & Shannon, 1927). 147. nigrimanum Macquart, 1838. — 148–149. S. (Disculter) subgen. nov. 148. oviedoi Ramírez-Pérez, 1971. 149. rivasi Ramírez-Pérez, 1971. Scale bars = 0.2 mm.

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Figs 91–106 in Simulium (Trichodagmia) (Diptera, Simuliidae) phylogeny revisited: the Neotropical and Afrotropical connection

Figs 91–106. Gills of pupae. 91–94. Simulium (Anasolen) Enderlein, 1930. 91. bisnovem Gibbins, 1938. 92. dentulosum Roubaud, 1915. 93. imerinae Roubaud, 1905. 94. masabae Gibbins, 1934. — 95– 97. S. (Freemanellum) Crosskey, 1969. 95. debegene De-Meillon, 1934. 96. manense Elsen & Escaffre, 1976. 97. hessei Gibbins, 1941. — 98. S. (Hearlea) canadense Hearle, 1932. — 99–102. S. (Hemicnetha) Enderlein, 1934. 99. cristalinum Coscarón & Py-Daniel, 1989. 100. earlei Vargas et al., 1946. 101. paynei Vargas, 1942. 102. virgatum Coquillett, 1902. —103. S. (Obuchovia) galloprovinciale Giudicelli, 1963. — 104–106. S. (Trichodagmia) Enderlein, 1934. 104. nigrimanum Macquart, 1838. 105. hirtipupa Lutz, 1910. 106. scutistriatum Lutz, 1909. Scale bars: 91–104, 106 = 0.2 mm; 105 = 0.05 mm.

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Figs 50–70. Males. Gonocoxite and gonostyle. 50–54 in Simulium (Trichodagmia) (Diptera, Simuliidae) phylogeny revisited: the Neotropical and Afrotropical connection

Figs 50–70. Males. Gonocoxite and gonostyle. 50–54. Simulium (Anasolen) Enderlein, 1930. 50. dentulosum Roubaud, 1915. 51. imerinae Roubaud, 1905. 52. kauntzeum Gibbins, 1938. 53. masabae Gibbins, 1934. 54. nili Gibbins, 1934. — 55–56. S. (Freemanellum) Crosskey, 1969. 55. hirsutilateris De-Meillon, 1937. 56. manense Elsen & Escaffre, 1976. — 57–59. S. (Hearlea) Vargas et al. 1946. 57. ayrozai Vargas, 1945. 58. canadense Hearle, 1932. 59. capricorne De León, 1945. — 60–62. S. (Hemicnetha) Enderlein, 1934. 60. brachycladum Lutz & Pinto, 1932. 61. cristalinum Coscarón & Py-Daniel, 1989. 62. tarsatum Macquart, 1846. — 63. S. (Shewellomyia) claricentrum Adler, 1990. — 64–68. S. (Trichodagmia) Enderlein, 1934. 64. guianense Wise, 1911. 65. hirtipupa Lutz, 1910. 66. itaunense D'Andretta & Dolores González, 1964. 67. lahillei (Paterson & Shannon, 1927). 68. nigrimanum Macquart, 1838. — 69–70. S. (Disculter) subgen. nov. 69. rivasi Ramírez-Pérez, 1971. 70. oviedoi Ramírez-Pérez, 1971. Scale bars = 0.1 mm.

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Figs 33–49. Males. Ventral plate. 33–37 in Simulium (Trichodagmia) (Diptera, Simuliidae) phylogeny revisited: the Neotropical and Afrotropical connection

Figs 33–49. Males. Ventral plate. 33–37. Simulium (Anasolen) Enderlein, 1930. 33. dentulosum Roubaud, 1915. 34. imerinae Roubaud, 1905. 35. kauntzeum Gibbins, 1938. 36. masabae Gibbins, 1934. 37. nili Gibbins, 1934. — 38. S. (Freemanellum) debegene De-Meillon, 1934. — 39– 40. S. (Hearlea) Vargas et al. 1946. 39. ayrozai Vargas, 1945. 40. larvispinosum De León, 1948. — 41–42. S. (Hemicnetha) Enderlein, 1934. 41. bricenoi Vargas et al., 1946. 42. tarsatum Macquart, 1846. — 43. S. (Shewellomyia) claricentrum Adler, 1990. — 44. S. (Obuchovia) galloprovinciale Giudicelli, 1963. — 45–47. S. (Trichodagmia) Enderlein, 1934. 45. itaunense D'Andretta & Dolores González, 1964. 46. lahillei (Paterson & Shannon, 1927). 47. scutistriatum Lutz, 1909. — 48–49. S. (Disculter) subgen. nov. 48. rivasi Ramírez-Pérez, 1971. 49. oviedoi Ramírez-Pérez, 1971. Scale bars = 0.1 mm.

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Figs 3–15. Females. 3–8. Thorax, dorsal view. 3–4 in Simulium (Trichodagmia) (Diptera, Simuliidae) phylogeny revisited: the Neotropical and Afrotropical connection

Figs 3–15. Females. 3–8. Thorax, dorsal view. 3–4. Simulium (Anasolen) Enderlein, 1930. 3. neireti Roubaud, 1905. 4. nili Gibbins, 1934. — 5. S. (Freemanellum) berghei Fain, 1949. — 6. S. (Hearlea) canadense Hearle, 1932. — 7. S. (Shewellomyia) pictipes Hagen, 1880. — 8. S. (Obuchovia) margaritae Rubtsov, 1956. — 9–15. Cibarium of females. 9–12. S. (Hemicnetha) Enderlein, 1934. 9. brachycladum Lutz & Pinto, 1932. 10. cristalinum Coscarón & Py-Daniel, 1989. 11. tarsatum Macquart, 1846. 12. pulverulentum Knab, 1915. — 13–15. S. (Trichodagmia) Enderlein, 1934. 13. lahillei (Paterson & Shannon, 1927). 14. nigrimanum Macquart, 1838. 15. scutistriatum Lutz, 1909. Scale bars: 3–8 = 0.25 mm; 9–12, 14 = 0.05 mm; 13, 15 = 0.02 mm.

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Fig. 2 in Simulium (Trichodagmia) (Diptera, Simuliidae) phylogeny revisited: the Neotropical and Afrotropical connection

Fig. 2. Strict consensus of the 16 most parsimonious trees that resulted from the analysis under Equal Weights (Fit = 16.87, Ci = 0.22, Ri = 0.74). Blue terminal taxa are the ingroup species included in the analysis. Colored branches show the proposed classification. Simulium (Disculter) subgen. nov. correspond to old Oviedoi species-group.

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Figs 107–127. 107–113 in Simulium (Trichodagmia) (Diptera, Simuliidae) phylogeny revisited: the Neotropical and Afrotropical connection

Figs 107–127. 107–113. Larvas (last instar). 107–108. Simulium (Anasolen) Enderlein, 1930. 107. dentulosum Roubaud, 1915. 108. imerinae Roubaud, 1905. — 109. S. (Freemanellum) manense Elsen & Escaffre, 1976. — 110. S. (Hearlea) canadense Hearle, 1932. —111. S. (Hemicnetha) paynei Vargas, 1942. — 112. S. (Shewellomyia) pictipes Hagen, 1880. — 113. S. (Obuchovia) auricoma Meigen, 1818. — 114–126. Last instar larvae. Head, dorsal view. 114–118. S. (Anasolen). 114. ambositrae Grenier & Grjebine, 1959. 115. dentulosum. 116. imerinae. 117. masabae Gibbins, 1934. 118. nili Gibbins, 1934. — 119–120. S. (Freemanellum). 119. manense. 120. hirsutilateris De-Meillon, 1937. — 121. S. (Hearlea) canadense. — 122–123. S. (Hemicnetha). 122. brachycladum Lutz & Pinto, 1932. 123. paynei. — 124–125. S. (Shewellomyia). 124. claricentrum Adler, 1990. 125. pictipes. — 126. S. (Obuchovia) ibericum Crosskey & Santos Grácio, 1985. — 127. Antenna of S. (Anasolen) ambositrae. Scale bars: 107–113 = 1 mm; 114–126 = 0.5 mm; 127 = 0.2 mm.

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Fig. 1 in Simulium (Trichodagmia) (Diptera, Simuliidae) phylogeny revisited: the Neotropical and Afrotropical connection

Fig. 1. Strict consensus of the three most parsimonious trees that resulted from the analysis under Implied Weights with K = 3 (Fit = 26.33977; Ci = 0.24; Ri = 0.75). Bremer support values (> 50) mapped above branches and bootstrap values over 50% are given below the nodes. Blue numbers on nodes (1–21) represent clades. Blue terminal taxa are the original ingroup included in the analysis. Colored branches show the proposed classification. Simulium (Disculter) subgen. nov. correspond to old Oviedoi species-group.

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Figs 16–32. Females. 16–21. Hypoginial valves. 16–17 in Simulium (Trichodagmia) (Diptera, Simuliidae) phylogeny revisited: the Neotropical and Afrotropical connection

Figs 16–32. Females. 16–21. Hypoginial valves. 16–17. Simulium (Anasolen) Enderlein, 1930. 16. dentulosum Roubaud, 1915. 17. neireti Roubaud, 1905. — 18. S. (Freemanellum) berghei Fain, 1949. — 19–20. S. (Hemicnetha) Enderlein, 1934. 19. cristalinum Coscarón & Py-Daniel, 1989. 20. virgatum Coquillett, 1902. — 21. S. (Trichodagmia) scutistriatum Lutz, 1909. — 22–32. Anal lobe and cercus. 22–23. S. (Anasolen). 22. masabae Gibbins, 1934. 23. neireti. — 24. S. (Freemanellum) hirsutilateris De-Meillon, 1937.— 25. S. (Hearlea) ayrozai Vargas, 1945. — 26. S. (Hemicnetha) tarsatum Macquart, 1846. — 27. S. (Shewellomyia) claricentrum Adler, 1990. — 28–30. S. (Trichodagmia). 28. guianense Wise, 1911. 29. lahillei (Paterson & Shannon, 1927). 30. scutistriatum. — 31–32. S. (Disculter) subgen. nov. 31. rivasi Ramírez-Pérez, 1971. 32. oviedoi Ramírez-Pérez, 1971. Scale bars: 16–21 = 0.1 mm; 22–32 = 0.05 mm.

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Figs 71–90. Pupae. 71–75 in Simulium (Trichodagmia) (Diptera, Simuliidae) phylogeny revisited: the Neotropical and Afrotropical connection

Figs 71–90. Pupae. 71–75. Simulium (Anasolen) Enderlein, 1930. 71. ambositrae Grenier & Grjebine, 1959. 72. dentulosum Roubaud, 1915. 73. imerinae Roubaud, 1905. 74. iphias De-Meillon, 1951. 75. nili Gibbins, 1934. — 76–77. S. (Freemanellum) Crosskey, 1969. 76. debegene De-Meillon, 1934. 77. manense Elsen & Escaffre, 1976. — 78–79. S. (Hearlea) Vargas et al. 1946. 78. canadense Hearle, 1932. 79. capricorne De León, 1945. — 80–81. S. (Hemicnetha) Enderlein, 1934. 80. paynei Vargas, 1942. 81. rubrithorax Lutz, 1909. — 82–83. S (Shewellomyia) Peterson, 1975. 82. claricentrum Adler, 1990. 83. pictipes Hagen, 1880. — 84–85. S. (Obuchovia) Rubtsov, 1947. 84. albellum Rubtsov, 1947. 85. galloprovinciale Giudicelli, 1963. — 86–88. S. (Trichodagmia) Enderlein, 1934. 86. nigrimanum Macquart, 1838. 87. orbitale Lutz, 1910. 88. scutistriatum Lutz, 1909. — 89–90. S. (Disculter) subgen. nov. 89. rivasi Ramírez-Pérez, 1971. 90. oviedoi Ramírez-Pérez, 1971. Scale bars = 1 mm.

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