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232 results for “cell membrane”
Datasets generated for the manuscript: The basement membrane regulates the cellular localization and the cytoplasmic interactome of Yes-Associated Protein (YAP) in mammary epithelial cells
<p><strong>File proteinGroups-CoIP-Yap1:</strong> Dataset of co-Immunoprecipitation followed of Yes-associated protein (YAP) followed by proteomics to identify YAP interactants.</p> <p><strong>File Gene_set_file_YAP: </strong>Gene sets used for gene set enrichment analysis.</p> <p><strong>Files enrichr_x: </strong>output of the EnrichR tool</p>
Data for 'Membrane marker selection for segmenting single cell spatial proteomics data'
<p>Additional data for 'Membrane marker selection for segmenting single cell spatial proteomics data'</p>
Negative E-cadherin expression on bone marrow myeloma cell membranes is associated with extramedullary disease
<p><span><strong>Background:</strong> </span><span>The loss of E-cadherin expression and the induction of N-cadherin are known as hallmarks of the epithelial-to-mesenchymal transition, an essential initial step in the process of metastasis in solid tumors. Although several studies have reported expressions of these cadherins in patients with multiple myeloma (MM), their clinical significance is unknown as MM cells are non-epithelial. </span></p> <p><strong><span>Methods:</span></strong><span> In this study, we examined the expression of E- and N-cadherins by immunohistochemistry using bone marrow (BM) biopsy specimens from 31 newly diagnosed MM patients and in subsequent biopsy specimens from six of these. </span></p> <p><strong><span>Results:</span></strong><span> Negative E-cadherin expression on BM myeloma cell membranes was significantly associated with the presence of soft-tissue masses arising from bone lesions and breaking through the cortical bone, referred to as extramedullary disease (EMD).</span></p> <p><strong><span>Conclusions:</span></strong><span><strong> </strong>Given the aggressive nature of EMD, our study suggests that screening for E-cadherin using BM immunohistochemistry is one measure that could predict the development of EMD in patients with MM.</span></p>
Raw gel and membrane figures associated with the publication "Iron-loaded deferiprone can support full hemoglobinization of cultured red blood cells"
<p>Raw gel and membrane figures associated with the publication <strong>Iron-loaded deferiprone can support full hemoglobinization of cultured red blood cells</strong>.</p> <p> </p> <p><strong>For Figure 1a:</strong></p> <p>Raw uncropped image of the gel run with samples combining apotransferrin with iron chelators. After staining with Coomassie, gel was scanned in an Epson V500 scanner (full color, 1200 dpi, bit depth = 24).</p> <p> </p> <p><strong>For Figure 3:</strong></p> <p>Raw uncropped images of the membranes obtained from western blots of cultured erythroblasts derived from three different donors, showing the expression level of proteins involved in iron metabolism regulation (ferritin, transferrin receptor, total EIF2, phospho-EIF2). Actin was used as housekeeping protein for relative quantification of protein abundance for further analysis.</p> <p>After staining, membranes were scanned in an Epson V500 scanner (full color, 1200 dpi, bit depth = 24).</p> <p>For all membranes, the loading order is the same:</p> <p>- <strong>Day 0:</strong> 1000 μg/mL holotransferrin</p> <p>- <strong>Day 0:</strong> 100 μg/mL holotransferrin</p> <p>- <strong>Day 0:</strong> 100 μg/mL holotransferrin + 52 μM iron-loaded deferiprone</p> <p>- <strong>Day 1:</strong> 52 μM iron-loaded deferiprone</p> <p>- <strong>Day 1:</strong> 1000 μg/mL apotransferrin</p> <p>- <strong>(empty lane)</strong></p> <p>- <strong>Day 1:</strong> 1000 μg/mL holotransferrin</p> <p>- <strong>Day 1:</strong> 100 μg/mL holotransferrin</p> <p>- <strong>Day 1:</strong> 100 μg/mL holotransferrin + 52 μM iron-loaded deferiprone</p> <p>- <strong>Day 1:</strong> 52 μM iron-loaded deferiprone</p> <p>- <strong>Day 1:</strong> 1000 μg/mL apotransferrin</p> <p>- <strong>(empty lane)</strong></p> <p>- <strong>Day 2:</strong> 1000 μg/mL holotransferrin</p> <p>- <strong>Day 2:</strong> 100 μg/mL holotransferrin</p> <p>- <strong>Day 2:</strong> 100 μg/mL holotransferrin + 52 μM iron-loaded deferiprone</p> <p>- <strong>Day 2:</strong> 52 μM iron-loaded deferiprone</p> <p>- <strong>Day 2:</strong> 1000 μg/mL apotransferrin</p> <p>More details on the protocol for western blot available in the manuscript at https://doi.org/10.1101/2021.08.02.454758</p> <p> </p> <p><strong>For Figure 4:</strong></p> <p>Raw uncropped images of the membranes obtained from western blots of cultured erythroblasts derived from two different donors with different iron supplementations (no hTf or Def, 52 μM iron-loaded deferiprone, or 300 μg/mL holotransferrin), showing the phosphorylation level of STAT5 upon withdrawal of erythropoietin (Epo) (3 hours) and restimulation with 0.2 or 1.0 U/mL Epo.</p> <p>After staining, membranes were scanned in an Epson V500 scanner (full color, 1200 dpi, bit depth = 24).</p> <p>For both membranes, the loading order is the same:</p> <p>- <strong>Lane 1:</strong> cells cultured without hTf nor Def, and no Epo restimulation</p> <p>- <strong>Lane 2:</strong> cells cultured with 52 μM iron-loaded deferiprone, and no Epo restimulation</p> <p>- <strong>Lane 3:</strong> cells cultured with 300 μg/mL holotransferrin, and no Epo restimulation</p> <p>- <strong>Lane 4:</strong> cells cultured without hTf nor Def, and restimulation with 0.2 U/mL Epo.</p> <p>- <strong>Lane 5:</strong> cells cultured with 52 μM iron-loaded deferiprone, and restimulation with 0.2 U/mL Epo.</p> <p>- <strong>Lane 6:</strong> cells cultured with 300 μg/mL holotransferrin, and restimulation with 0.2 U/mL Epo.</p> <p>- <strong>Lane 7:</strong> cells cultured without hTf nor Def, and restimulation with 1.0 U/mL Epo.</p> <p>- <strong>Lane 8:</strong> cells cultured with 52 μM iron-loaded deferiprone, and restimulation with 1.0 U/mL Epo.</p> <p>- <strong>Lane 9:</strong> cells cultured with 300 μg/mL holotransferrin, and restimulation with 1.0 U/mL Epo.</p>
Dataset - Membrane-localized magnetic hyperthermia promotes intracellular delivery of cell-impermeant probes
<p><span>Data regarding the MNP surface temperature estimation, magnetic measurements, and qPCR data for the HSP expression.</span></p> <p><span>The data set is related to the article "Membrane-localized magnetic hyperthermia promotes intracellular delivery of cell-impermeant probes", submitted to Nanoscale</span></p>
OpenFoam model output for "A Fuel Cell Power Supply System Equipped with Artificial Gill Membranes for Underwater Applications"
<p>Dataset of numerical experiments carried out with OpenFOAM v 10 as used in the manuscript "A Fuel Cell Power Supply System Equipped with Artificial Gill Membranes for Underwater Applications" by Lucas Merckelbach and Prokopios Georgopanos.</p> <p> </p>
Data from: "Glycerol-blended chitosan membranes with directional micro-grooves and reduced stiffness improve Schwann cell wound healing"
<h3>ABSTRACT</h3> <p>Regenerative medicine is continuously looking for new natural biocompatible and possibly biodegradable materials, but also mechanically compliant. Chitosan is emerging as a promising FDA-approved biopolymer for tissue engineering, however, its exploitation in regenerative devices is limited by its brittleness and can be further improved, for example, by blending it with other materials or by tuning its superficial microstructure. Here, we developed membranes made of chitosan and glycerol, by solvent casting and micropatterned them with directional geometries with different levels of axial symmetry. These membranes were characterized by light microscopy and atomic force microscopy (AFM), thermal, mechanical, and degradation assays, and also tested in vitro as scaffolds with Schwann cells. The glycerol-blended chitosan membranes are optimized in terms of mechanical properties, and present a physiological-grade Young's modulus (≈ 0.7 MPa). The directional topographies are effective in directing cell polarization and migration and in particular are highly performant substrates for collective cell migration. Here, we demonstrate that a combination of a soft compliant biomaterial and topographical micropatterning can improve the integration of these scaffolds with Schwann cells, which is a fundamental step in the peripheral nerve regeneration process.</p>
Si3N4 membrane breaks in ultrahigh vacuum during operando NEXAFS in solar hydrogen water splitting micro electrochemical cell
<p>This video was recorded 19 April 2012 during operando photoelectrochemical water splitting in a micro specroelectrochemical x-ray cell during NEXAFS beamtime at the Advanced Light Source in Berkeley. The window broke in the UHV chamber, when the potential was reversed with the potentiostat from 900 mV to lower potentials. Fortunately, we had already the light O1s x-ray absorption spectra in light on/off mode for the potentials up to 900 mV.</p>
Piezo1 as a force-through-membrane sensor in red blood cells
<p>Piezo1 is the stretch-activated Ca<sup>2+</sup> channel in red blood cells that mediates homeostatic volume control. Here we study the organization of Piezo1 in red blood cells using a combination of super-resolution microscopy techniques and electron microscopy. Piezo1 adopts a nonuniform distribution on the red blood cell surface, with a bias towards the biconcave "dimple". Trajectories of diffusing Piezo1 molecules, which exhibit confined Brownian diffusion on short timescales and hopping on long timescales, also reflect a bias towards the dimple. This bias can be explained by "curvature coupling" between the intrinsic curvature of the Piezo dome and the curvature of the red blood cell membrane. Piezo1 does not form clusters with itself, nor does it co-localize with F-actin, Spectrin or the Gardos channel. Thus, Piezo1 exhibits the properties of a force-through-membrane sensor of curvature and lateral tension in the red blood cell.</p>
Data for "Coordination of bacterial cell wall and outer membrane biosynthesis"
<p>Raw data used in the paper "Coordination of bacterial cell wall and outer membrane biosynthesis" by Katherine R. Hummels, Samuel P. Berry, Zhaoqi Li, Atsushi Taguchi, Joseph K. Min, Suzanne Walker, Debora S. Marks, and Thomas G. Bernhardt. These data were collected with the aim of understanding how the lipopolysaccharide biosynthetic enzyme LpxC is regulated in diverse gram-negative bacteria, particularly Pseudomonas aeruginosa. The dataset contains the following tarred directories:</p> <p><em><strong>Experimental data</strong></em></p> <ul> <li>Microscopy (microscopy.tar.gz)</li> <li>LC-MS/MS (lc-ms_ms.tar.gz)</li> </ul> <p><strong><em>Covariation analysis</em></strong></p> <ul> <li>Multiple sequence alignments (alignments.tar.gz)</li> <li>AlphaFold structures (alphafold.tar.gz)</li> <li>EVcomplex models and couplings (evcomplex.tar.gz)</li> <li>Phylogenetic trees (trees.tar.gz)</li> </ul> <p>For more detailed methods and file descriptions, please see README.md. Associated code for analysis can be found at https://github.com/samberry19/evcomplex-interaction-scoring.</p>
Circulating Tumor Cells (CTC) Before and After Thoracic Resection With and Without Intraoperative Use of ExtraCorporeal Membrane Oxygenator(ECMO) or Cardio Pulmonary By Pass (CPB)
ClinicalTrials.gov study NCT04048512. IPD Sharing: NO. Countries: 1. Publications: 10.
Study of the Cellular Diffusion of Tacrolimus Across the Membrane of Mononuclear Cells
ClinicalTrials.gov study NCT03654794. IPD Sharing: UNDECIDED. Countries: 1. Publications: 2.
Analysis of T- and B-Cell Subpopulations in Membranous Nephropathy
ClinicalTrials.gov study NCT05894512. IPD Sharing: NO. Countries: 1. Publications: 2.
Effect of B-cell Depleting Therapies on PLA2R-specific B Cells in Patients With Membranous Nephropathy
ClinicalTrials.gov study NCT06994468. IPD Sharing: NO. Countries: 1. Publications: 3.
Effects of 5-Methyltetrahydrofolate and Vitamin B12 Supplemetation on Red Cell Membrane in Children With Cystic Fibrosis
ClinicalTrials.gov study NCT00730509. IPD Sharing: Not stated. Countries: 1. Publications: 2.
The Effect of Alpha-tocopherol in Hemolysis and Oxidative Stress Marker on the Red Cell Membrane Beta-thalassemia Major
ClinicalTrials.gov study NCT03948737. IPD Sharing: NO. Countries: 1. Publications: 8.
Mensenchymal Stem Cell (MSC) Included in OrthADAPT Membrane for Rotator Cuff Tears Repair
ClinicalTrials.gov study NCT01687777. IPD Sharing: Not stated. Countries: 1. Publications: 26.
Results from: Angiogenic property of silk fibroin scaffolds with adipose-derived stem cells on chick chorioallantoic membrane
Open the record for dataset details and reuse information.
Piezo1 as a force-through-membrane sensor in red blood cells
Open the record for dataset details and reuse information.
Negative E-cadherin expression on bone marrow myeloma cell membranes is associated with extramedullary disease
Open the record for dataset details and reuse information.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.