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473 results for “confocal”
Fig. 11. Confocal laser scanning 3D reconstructions. A in Living on the edge - first survey of loriciferans along the Atacama Trench
Fig. 11. Confocal laser scanning 3D reconstructions. A. Pliciloricus leocaudatus, ventral view, paratype (NHMD 100760). B. Pliciloricus aff. ukupachaensis collected off Oregon, US west coast, lateral view (NHMD 225904).
Confocal Raman spectroscopy data from native and polyethylene glycol-containing wood
<p>Confocal Raman mapping data from native pine wood and pine wood with polyethylene glycol (PEG) of three different molecular weights in deuterated water.</p> <p>The spectroscopic data consists of 4 text files, each of which contains in total 1600 2D arrays of Raman intensity corresponding to different wavenumber values. Each of these 2D arrays consists of 175 rows with 175 comma-separated values on each row. The 2D arrays corresponding to different wavenumbers are separated by a line starting with '#' and specifying the wavenumber of the subsequent 2D array (unit inverse cm). The wavenumber axis is also given in a separate file. The image size is 45 µm x 45 µm (175 x 175 pixels) and the wavenumber axis consists of 1600 points.</p> <p>For further details on the samples and data collection, see the following reference:<br> Paavo A. Penttilä, Michael Altgen, Muhammad Awais, Monika Österberg, Lauri Rautkari, & Ralf Schweins. Bundling of cellulose microfibrils in native and polyethylene glycol-containing wood cell walls revealed by small-angle neutron scattering. <em>Scientific Reports</em> <strong>10, </strong>20844 (2020). https://doi.org/10.1038/s41598-020-77755-y</p>
Confocal and micro-CT data of Miniomma chenkuni, holotype, NIGP173375
<p>This dataset contains the raw confocal laser scanning microscopy (CLSM) slices and X-ray microtomography (micro-CT) slices for the holotype of <em>Miniomma chenkuni</em> (NIGP173375) from mid-Cretaceous Burmese amber. This dataset is associated with the publication "A miniaturized ommatid beetle in mid-Cretaceous Burmese amber (Coleoptera: Archostemata: Ommatidae)" (DOI:10.11606/1807-0205/2020.60.63).</p> <p>Confocal images were obtained with a Zeiss LSM710 confocal laser scanning microscope, using 488 nm Argon laser excitation line. Both original CZI files and exported TIFF stacks are provided.</p> <p>Micro-CT data were obtained with a Zeiss Xradia 520 Versa 3D X-ray microscope. Scanning parameters were as follows: isotropic voxel size, 2.1437 μm; power, 3 W; acceleration voltage, 40 kV; exposure time, 4 s; projections, 3001. The TIFF stack is provided.</p>
Confocal microscopy findings of K17 in OLP.
<p>Raw data of figure 7.</p>
Super-resolved Reflectance Confocal Microscopy 3D reconstruction of a Diatom shell
<p>These movies present back-to-back the performance in term of resolution of both standard reflectance confocal microscopy and super-resolved rescanned reflectance confocal microscopy. One movie is a 3D stack angular rotation, the other is a Zstack. </p> <p> </p>
Super-resolved Reflectance Confocal Microscopy data on Diatom shells and live MEF cells
<p>This dataset is linked to a paper submission in Open Research Europe. It contains the RAW data of confocal reflectance super-resolved point spread function, diatom shell zstack and time lapse imaging of a living Mouse Embryonic Fibroblast cell. </p>
Super-resolved Reflectance Confocal Microscopy time-lapse imaging of a living MEF cell lamellipod
<p>This movies presents a time-lapse of a label-free living Mouse embryonic fibroblast cell observed with super-resolved rescanned reflectance confocal microscopy.. </p>
Confocal data of Captopus depressiceps, Electrothroscus yanpingae, and Pseudopactopus robustus
<p>This dataset contains the raw confocal laser scanning microscopy (CLSM) slices for the specimens of <em>Captopus depressiceps</em> (NIGP173915), <em>Electrothroscus yanpingae</em> (NIGP173916) and <em>Pseudopactopus robustus</em> (NIGP173917 and NIGP173918) from mid-Cretaceous Burmese amber. This dataset is associated with the publication "New genera and species of the family Throscidae (Coleoptera: Elateroidea) in mid-Cretaceous Burmese amber" (DOI:10.3390/insects12010063).</p> <p>Confocal images were obtained with a Zeiss LSM710 confocal laser scanning microscope, using 488 nm Argon laser excitation line. Both original CZI files and exported TIFF stacks are provided.</p>
Confocal data of Cretophengodes azari, holotype, NIGP173775
<p>This dataset contains the raw confocal laser scanning microscopy (CLSM) slices for the holotype of <em>Cretophengodes azari</em> (NIGP173775) from mid-Cretaceous Burmese amber. This dataset is associated with the publication "Cretophengodidae, a new Cretaceous beetle family, sheds light on the evolution of bioluminescence" (DOI:10.1098/rspb.2020.2730).</p> <p>Confocal images were obtained with a Zeiss LSM710 confocal laser scanning microscope, using 488 nm Argon laser excitation line. Both original CZI files and exported TIFF stacks are provided.</p>
V79 fibroblasts loaded with 50 nm PVP coated gold particles captures by confocal laser scanning microscopy
<p>Data was recorded continuously in a region of 41x41 μm² at a scan rate of 1400 Hz resulting in a frame duration of 94 ms; the pinhole was set to 600 μm (5.778 μm layer), image resolution was 256x256 pixel. (11 FPS)</p>
Entire confocal z-stack series as .tif image sequences
<p>The manuscript entitled "Parvalbumin-expressing ependymal cells in rostral lateral ventricle wall adhesions contribute to aging-related ventricle stenosis in mice" shows confocal z-stack maximum intensity projections and thin z-plane reconstructions in the figure plates. The entire confocal z-stack image series are provided here as .tif image sequences, respectively the confocal z-stacks of the negative controls as well. The file names refer to the figure numbers and position in the figure plates. For more information about the immunostaining and image acquisition, see the Materials & Methods and Figure legends in the manuscript.</p>
Dataset of confocal microscopy - Rhamnogalacturonan-II dimerization deficiency impairs the coordination between growth and adhesion maintenance in plants
<p>This contains additional data relative to version 1, corresponding to a new versio of the manuscript. </p> <p>This data set contains confocal images (3D stacks and 2D projections) from propidium iodide stained <em>Arabidopsis thaliana </em>dark grown hypocotyls of various wildtype and mutant plants reported in the study "Rhamnogalacturonan-II dimerization deficiency impairs the coordination between growth and adhesion maintenance in plants" (https://www.biorxiv.org/content/10.1101/2024.11.26.625362v1). Data was acquired following method described in the publication.</p> <p> </p>
Scan tiles obtained through Confocal Laser Scanning Microscopy for roughness characterization of surfaces
<p>The Data includes all the original data collected from individual scan tiles of both quartz and glass surfaces using Confocal Laser Scanning Microscopy. The techniques used for merging scan tiles linearly, as well as the procedures for data processing and analysis, are detailed in the methods and results sections of this manuscript. A sperate methods section include along with data files also describe the details of the Image acquisition, preprocessing, and tiling methodology.</p>
Confocal data of Mysteriomorphus sp., NIGP202848
<p>This dataset contains the raw confocal laser scanning microscopy (CLSM) slices for the female specimen of <i>Mysteriomorphus </i>sp. (NIGP202848) from mid-Cretaceous Burmese amber. This dataset is associated with the publication "Further mysteries solved in Mysteriomorphidae (Coleoptera: Elateroidea) from mid-Cretaceous amber of northern Myanmar: discovery of female specimens, and synonymy of Trapezioceps recently described as Cantharidae" (DOI:10.1016/j.cretres.2023.105756).</p><p>Confocal images were obtained with a Zeiss LSM710 confocal laser scanning microscope, using the 488 nm (Argon) laser excitation line. The original CZI files are provided, which could be opened by the ZEISS ZEN software.</p>
Phenotypic differences between interfertile Chlamydomonas species- high-resolution confocal z-stacks for visualizing organelle morphology
<p>This repository contains high-resolution confocal z-stacks of two interfertile <i>Chlamydomonas</i> algal species. The protocol to generate this data is described in the associated publication, "Phenotypic differences between interfertile <i>Chlamydomonas</i> species", and briefly summarized here. Cells were collected from agar plates with TAP medium and suspended in 500 µl of liquid TAP medium in a 1.5 ml eppendorf tube overnight. Cells were pelleted using a microcentrifuge at 2000 x g for 2 min and the supernatant removed. For staining mitochondria, PKMito orange was used at a 1:500 concentration and cells were moved to opaque black microcentrifuge tubes and placed on a tube rotator for 45 min. Cells were pelleted again and washed twice with fresh TAP medium. After the final wash and supernatant removal, cells were resuspended in 25 µl of 1.25% low gelling agar in TAP medium (kept at 45 C). Then 1 µl of the cell/agar mixture was mounted on a #1.5 coverslip with a small wax circle drawn to retain the droplet. Coverslips were flipped and placed on a slide and sealed with VALAP. </p><p>Images were collected on a Nikon CSU W-1 SoRA spinning disk confocal microscope equipped with an ORCA-Fusion BT digital scMOS camera. In order to apply deconvolution in the downstream processing, we needed to oversample (sample beyond Nyquist) in z resolution. To do this, we used a 100×/1.45 NA objective in 2.8× SoRa magnification mode, using ROIs of either 670 × 670 × 81 or 850 × 850 × 91. We imaged with a z-step size of 100 nm for sub-Nyquist sampling. We imaged bright-field first, then 640 nm excitation autofluorescence of chloroplasts, and then 561 nm excitation for PKmito orange dye, because the chloroplasts would bleach after 561 nm excitation. We set exposures to 300 ms with 30% and 50% laser power for 640 and 561, respectively.</p><p>We have included a set of demo data (10 images per species) that accompany the pub hosted on the Arcadia Science webpage (3Dmorpho_demo_data). In addition, we included all of the raw data we collected in this experiment (3Dmorpho_raw_data). Please use the point spread functions (PSF) from the zipped folders for each respective dataset (demo or raw). </p>
Confocal stacks of Cii_beta gamma crystalin_hM4D(Gi)_mCherry transgenic Ciona Larvae
<p>Confocal stacks of Cii_beta gamma crystalin_hM4D(Gi)_mCherry transgenic Ciona Larvae used to generate the panels of Hoyer et al.</p>
Data file for Massé et al.'s article, "Unraveling the Life History of Past Populations through Hypercementosis: Insights into Cementum Apposition Patterns and Possible Etiologies using Micro-CT and Confocal Microscopy".
<p>This repository provides a supporting data file for the following research article:</p> <p>Massé L., d’Incau E., Souron A., Vanderesse N., Santos F., Maureille B., Le Cabec A. (2024) Unraveling the Life History of Past Populations through Hypercementosis: Insights into Cementum Apposition Patterns and Possible Etiologies Using Micro-CT and Confocal Microscopy. <em>Biology</em>, 13, 43. doi: <span><a href="https://doi.org/10.3390/biology13010043" target="_blank" rel="nofollow noopener noreferrer">10.3390/biology13010043</a></span></p> <p>For the detailed statistical analyses performed using this dataset, see Supporting Information 1 of the article.</p>
Source code and data for manuscript "Large-scale deep tissue voltage imaging with targeted illumination confocal microscopy"
<p>Source code and data for manuscript "Large-scale deep tissue voltage imaging with targeted illumination confocal microscopy", <em>Nat Methods</em> (2024), https://doi.org/10.1038/s41592-024-02275-w.</p>
Confocal data of Bulasconotus carinisternus, holotype, NIGP203566
<div>This dataset contains the raw confocal laser scanning microscopy (CLSM) slices for the holotype of <em>Bulasconotus carinisternus</em> (NIGP203566) from mid-Cretaceous Burmese amber. This dataset is associated with the publication "A new species of extant <em>Bulasconotus</em> from mid-Cretaceous Kachin amber (Coleoptera: Zopheridae: Colydiinae)" (DOI:10.11606/1807-0205/2024.64.015).</div> <div> </div> <div>Confocal images were obtained with a Zeiss LSM710 confocal laser scanning microscope, using the 488 nm (Argon) laser excitation line. The original CZI files are provided, which could be opened by the ZEISS ZEN software.</div>
Confocal data of Ripidinelia daiboyui, holotype, NIGP203880
<div>This dataset contains the raw confocal laser scanning microscopy (CLSM) slices for the holotype of <em>Ripidinelia daiboyui</em> (NIGP203880) from mid-Cretaceous Burmese amber. This dataset is associated with the publication "<em>Ripidinelia daiboyui</em> sp. nov. from mid-Cretaceous Kachin amber (Coleoptera: Ripiphoridae)" (DOI:10.11646/mesozoic.1.1.8).</div> <div> </div> <div>Confocal images were obtained with a Zeiss LSM710 confocal laser scanning microscope, using the 488 nm (Argon) or 561 nm (DPSS 561-10) laser excitation line. The original CZI files are provided, which could be opened by the ZEISS ZEN software.</div>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.