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FIGURE 5 in The taxonomy and phylogeny of the genus Cylindrospermopsis (Cyanobacterium) evaluated by adding five new records from China

FIGURE 5. ML phylogenetic tree based on the ITS-L sequences (510 bp) from thirty-six strains of six Cylindrospermopsis species described in the study, with Anabaena variabilis ATCC 29413 as the outgroup. Bootstrap values greater than 50% are given in front of the corresponding nodes for NJ and ML analyses followed by Bayesian posterior probabilities (over 0.5 are illustrated).

FIGURE 1 in The taxonomy and phylogeny of the genus Cylindrospermopsis (Cyanobacterium) evaluated by adding five new records from China

FIGURE 1. General morphological aspects of five Cylindrospermopsis new records species from China. A–C. C. taverae CHAB 143. D–G. C. helicoidea CHAB 357. H–K. C. philippinensis CHAB 5180. L. C. philippinensis CHAB 5182. M. C. catemaco CHAB 148. N–O. C. catemaco CHAB 151. P. C. catemaco CHAB 155. Q. C. catemaco CHAB 220. R. C. africana CHAB 359. S. C. africana CHAB 485. T–V. C. africana CHAB 1394. Scale bars = 10 μm. Note: C. = Cylindrospermopsis.

FIGURE 4 in The taxonomy and phylogeny of the genus Cylindrospermopsis (Cyanobacterium) evaluated by adding five new records from China

FIGURE 4. ML phylogenetic tree based on the 16S rRNA gene sequences (1268 bp) from thirty-six strains of six Cylindrospermopsis species described in the study, with Anabaena variabilis ATCC 29413 as the outgroup. Bootstrap values greater than 50% are given in front of the corresponding nodes for NJ and ML analyses followed by Bayesian posterior probabilities (over 0.5 are illustrated).

FIGURE 3 in The taxonomy and phylogeny of the genus Cylindrospermopsis (Cyanobacterium) evaluated by adding five new records from China

FIGURE 3. Maximum likelihood (ML) phylogenetic tree based on the 16S rRNA gene sequences from thirty-six strains of six Cylindrospermopsis species described in the study, together with sequences from other heterocytous cyanobacteria obtained from NCBI. Bootstrap values greater than 50% are given in front of the corresponding nodes for neighbor-joining (NJ) and ML analyses followed by Bayesian posterior probabilities (over 0.5 are illustrated).

FIGURE 6 in The taxonomy and phylogeny of the genus Cylindrospermopsis (Cyanobacterium) evaluated by adding five new records from China

FIGURE 6. ML phylogenetic tree based on the concatenated of four gene loci (16S rRNA, ITS, cpcBA-IGS and rpoC1) sequences (2864 bp) from thirty-six strains of six Cylindrospermopsis species described in the study, with Anabaena variabilis ATCC 29413 as the outgroup. Bootstrap values greater than 50% are given in front of the corresponding nodes for NJ and ML analyses followed by Bayesian posterior probabilities (over 0.5 are illustrated).

FIGURE 4 in Potamosiphon australiensis gen. nov., sp nov. (Oscillatoriales), a new filamentous cyanobacterium from subtropical north-eastern Australia

FIGURE 4. Phylogenetic tree based on the nifH gene sequence (312 bp) showing the phylogenetic position of Potamosiphon australiensis (shown in bold; scale bar = 0.05 nucleotide substitutions per site). Numbers indicate bootstrap values (>50%) from 1,000 replicates of neighbour-joining (NJ) and maximum likelihood (ML) analyses respectively. GenBank accession numbers are shown in parentheses.

FIGURE 1 in Potamosiphon australiensis gen. nov., sp nov. (Oscillatoriales), a new filamentous cyanobacterium from subtropical north-eastern Australia

FIGURE 1. Morphology of Potamosiphon australiensis (A–F) mature filaments with lamellated sheaths, (C–D) diagonal trichome fragmentation, (B, E, G) trichome fragmentation following formation of necridia; scale bar = 30 μm.

FIGURE 3 in Potamosiphon australiensis gen. nov., sp nov. (Oscillatoriales), a new filamentous cyanobacterium from subtropical north-eastern Australia

FIGURE 3. Phylogenetic tree based on the 16S rRNA gene sequence (1406–1412 bp) showing the phylogenetic position of Potamosiphon australiensis along with sequences from other taxa within the Oscillatoriales (scale bar = 0.02 nucleotide substitutions per site). Numbers indicate bootstrap values (>50%) from 1,000 replicates of neighbour-joining (NJ) and maximum likelihood (ML) analyses respectively. GenBank accession numbers are shown in parentheses.

FIGURE 5 in Potamosiphon australiensis gen. nov., sp nov. (Oscillatoriales), a new filamentous cyanobacterium from subtropical north-eastern Australia

FIGURE 5. Phylogenetic tree based on the 16S–23S rRNA operon (1971–2089 bp) showing the phylogenetic position of Potamosiphon australienses (shown in bold; scale bar = 0.02 nucleotide substitutions per site). Numbers indicate bootstrap values (>50%) from 1,000 replicates of neighbour-joining (NJ) and maximum likelihood (ML) analyses respectively. GenBank accession numbers are shown in parentheses.

FIGURE 2 in Potamosiphon australiensis gen. nov., sp nov. (Oscillatoriales), a new filamentous cyanobacterium from subtropical north-eastern Australia

FIGURE 2. Transmission electron micrographs of transverse (A) and longitudinal sections (B–C) of Potamosiphon australiensis showing location of thylakoids (th), cyanophycin granules (cy), cell wall (cw), and sheath (sh); scale bar = 5 μm.

FIGURE 1 in Nodosilinea chupicuarensis sp. nov. (Leptolyngbyaceae, Synechococcales) a subaerial cyanobacterium isolated from a stone monument in central Mexico

FIGURE 1. Sampling site at the Archaeological zone of Cañada de la Virgen. A. Aerial view of the Complex A (image taken from Google Maps, 2016). B. South drainage channel of the Complex A central-courtyard. C. Complex A central-courtyard. Red arrows indicate the specific sampling point.

FIGURE 5 in Nodosilinea chupicuarensis sp. nov. (Leptolyngbyaceae, Synechococcales) a subaerial cyanobacterium isolated from a stone monument in central Mexico

FIGURE 5. Unrooted tree of Nodosilinea strains based on Bayesian Analysis of 19 sequences (562 DNA positions, 114 coded indels). Bootstrap values from the parsimony analysis (562 DNA positions, gaps coded as a fifth base) are mapped onto the nodes in which the analyses were in agreement.

FIGURE 6 in Nodosilinea chupicuarensis sp. nov. (Leptolyngbyaceae, Synechococcales) a subaerial cyanobacterium isolated from a stone monument in central Mexico

FIGURE 6. Secondary structure of conserved domains of the 16S-23S ITS regions for 8 representative strains of Nodosilinea. A–H: D1- D1' helices; I–P: Box-B helices; Q: V3 helix (all 19 strains). Strain labels in the bottom row apply to the D1-D1' helices in the first row. Bases differing from those in N. chupacuarensis are indicated by hollow circles next to the differing bases.

FIGURE 3 in Nodosilinea chupicuarensis sp. nov. (Leptolyngbyaceae, Synechococcales) a subaerial cyanobacterium isolated from a stone monument in central Mexico

FIGURE 3. SEM micrographs of Nodosilinea chupicuarensis. A. Mature uniseriate filament forming a tight spiral. B. Amplification of a spiral. C. Coiled filaments.

FIGURE 2 in Nodosilinea chupicuarensis sp. nov. (Leptolyngbyaceae, Synechococcales) a subaerial cyanobacterium isolated from a stone monument in central Mexico

FIGURE 2. Main characteristics of Nodosilinea chupicuarensis. A-C. Mature filaments forming loose to tight spirals. D. A characteristic nodule (arrow). E. Multiseriate filament. F. Filaments with mature, elongated end cells (arrows). All figures to same scale, scale = 10 μm.

FIGURE 4 in Nodosilinea chupicuarensis sp. nov. (Leptolyngbyaceae, Synechococcales) a subaerial cyanobacterium isolated from a stone monument in central Mexico

FIGURE 4. Phylogenetic position of Nodosilinea chupicuarensis (denoted with an arrow) in a Bayesian Analysis (285 OTUs, 1222 positions), with support values on nodes representing BA posterior support/ML bootstrap support/MP bootstrap support, respectively. The uncollapsed tree from which this figure was derived appears in supplemental materials.

FIGURE 8 in New cyanobacterium Nodosilinea svalbardensis sp. nov. (Prochlorotrichaceae, Synechococcales) isolated from alluvium in Mimer river valley of the Svalbard archipelago

FIGURE 8. Color-coded secondary structure of Box-B helices and alignment of the selected set of sequences of Nodosilinea. The black color with white letters indicates conservative regions, gray areas denotes variable parts. Notice that sequences of the terminal loop are placed in the boxes within the alignment

FIGURE 6. Bayesian 16S-23S in New cyanobacterium Nodosilinea svalbardensis sp. nov. (Prochlorotrichaceae, Synechococcales) isolated from alluvium in Mimer river valley of the Svalbard archipelago

FIGURE 6. Bayesian 16S-23S ITS rRNA phylogeny showing the position of the Nodosilinea svalbardensis. Symbol "-" show support less than 50% on representative nodes. Taxa in the quotation mark needs to be revised. Note type species of Nodosilinea, N. nodulosa UTEX 2910.

FIGURE 7 in New cyanobacterium Nodosilinea svalbardensis sp. nov. (Prochlorotrichaceae, Synechococcales) isolated from alluvium in Mimer river valley of the Svalbard archipelago

FIGURE 7. Color-coded secondary structure of D1-D1' helices of the 16S-23S ITS region and its alignment for 5 representative species of Nodosilinea. Circles near N. svalbardensis indicate differences with closely related N. bijuigata.

FIGURE 4 in New cyanobacterium Nodosilinea svalbardensis sp. nov. (Prochlorotrichaceae, Synechococcales) isolated from alluvium in Mimer river valley of the Svalbard archipelago

FIGURE 4. Line drawings of Nodosilinea svalbardensis. A. Culture material. B. Natural populations. Note clearly visible sheaths in the natural populations. Numbers indicate morphological features: 1—false branching, 2—nodule, 3—attached hormogonia, 4—necridia, 5—granules.