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700 results for “ex vivo”
Datasets for "Insight into ductular reaction in obstructive biliary disease from a three-dimensional perspective using ex vivo X-ray phase contrast computed tomography"
<p>Phase-contrast CT of BDL rats liver-4 week</p>
Cardiac structure discontinuities revealed by ex-vivo microstructural characterization. A focus on the basal inferoseptal left ventricle region
<p>Data and materials regarding the submission of the paper. See Data Avaibility section and https://github.com/valeryozenne/Cardiac-Structure-Database for more information and code.</p>
Transcriptomic and spatial datasets of human ex vivo right atrial tissue in ischemic heart disease and heart failure
<p>This dataset contains raw counts and processed data and annotations for our transcriptomic and spatial dissection of human ex vivo right atrial tissue in ischemic heart disease and heart failure.</p> <p> </p> <p>snRNA.zip contains the snRNA-seq dataset for heart right atrial appendade and pericardial fluid.</p> <p>VISIUM.zip contains the Visium spatial transcriptomics data</p> <p>Molecular cartography.zip contains the Resolve Biosciences molecular cartography spatial transcriptomics data</p>
Ascorbic acid supports ex vivo generation of plasmacytoid dendritic cells from circulating hematopoietic stem cells: RNA-seq dataset
<p>Plasmacytoid dendritic cells (pDCs) constitute a rare type of immune cell with multifaceted functions, but their potential use as a cell-based immunotherapy is challenged by the scarce cell numbers that can be extracted from blood. Here, we systematically investigate culture parameters for generating pDCs from hematopoietic stem and progenitor cells (HSPCs). Using optimized conditions combined with implementation of HSPC pre-expansion, we generate an average of 465 million HSPC-derived pDCs (HSPC-pDCs) starting from 100,000 cord blood-derived HSPCs. Furthermore, we demonstrate that such protocol allows HSPC-pDC generation from whole blood HSPCs, and these cells display a pDC phenotype and function. Using GMP compliant medium, we observe a remarkable loss of TLR7/9 responses, which is rescued by ascorbic acid supplementation. Ascorbic acid induces transcriptional signatures associated with pDC-specific innate immune pathways suggesting an undescribed role of ascorbic acid for pDC functionality. This constitutes the first protocol for generating pDCs from whole blood, and lay the foundation for investigating HSPC-pDCs for cell-based immunotherapy.</p>
Treatment with 3-aminobenzamide during ex vivo lung perfusion of damaged rat lungs reduces graft injury and dysfunction after transplantation
<p>Ex vivo lung perfusion (EVLP) with pharmacological reconditioning may increase donor lung utilization for transplantation (LTx). 3-Aminobenzamide (3-AB), an inhibitor of poly (ADP-ribose) polymerase (PARP), reduces ex vivo lung injury in rat lungs damaged by warm ischemia (WI). Here we determined the effects of 3-AB reconditioning on graft outcome after LTx. Three groups of donor lungs were studied: Control (Ctrl): 1 hour WI + 3 hours cold ischemia (CI) + LTx; EVLP: 1 hour WI + 3 hours EVLP + LTx; EVLP + 3-AB: 1 hour WI + 3 hours EVLP + 3-AB (1 mg<sup>.</sup>mL<sup>−1</sup>) + LTx. Two hours after LTx, we determined lung graft compliance, edema, histology, neutrophil counts in bronchoalveolar lavage (BAL), mRNA levels of adhesion molecules within the graft, as well as concentrations of interleukin-6 and 10 (IL-6, IL-10) in BAL and plasma. 3-AB reconditioning during EVLP improved compliance and reduced lung edema, neutrophil infiltration, and the expression of adhesion molecules within the transplanted lungs. 3-AB also attenuated the IL-6/IL-10 ratio in BAL and plasma, supporting an improved balance between pro- and anti-inflammatory mediators. Thus, 3-AB reconditioning during EVLP of rat lung grafts damaged by WI markedly reduces inflammation, edema, and physiological deterioration after LTx, supporting the use of PARP inhibitors for the rehabilitation of damaged lungs during EVLP.</p>
Effects of cold or warm ischemia and ex-vivo lung perfusion on the release of damage associated molecular patterns and inflammatory cytokines in experimental lung transplantation
<p>Lung transplantation (LTx) is associated with sterile inflammation, possibly related to the release of damage associated molecular patterns (DAMPs) by injured allograft cells. We have measured cellular damage and the release of DAMPs and cytokines in an experimental model of LTx after cold or warm ischemia and examined the effect of pretreatment with ex-vivo lung perfusion (EVLP).</p>
Ex-vivo meritve - posnetki termalne kamere
<p>Posnetki termalne kamere tekom hipertermične laserske lipolize na homogenem in heterogenem svinjskem tkivu</p>
Cartilage Responses to Inflammatory Stimuli and Adipose Stem/Stromal Cell-Derived Conditioned Medium: Results from an Ex Vivo Model
Open the record for dataset details and reuse information.
Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing
<p>Human muscle tissue samples obtained from 31 patients was used in this in vitro analytical and experimental study. To optimize transduction protocol, samples from 28 patients were transduced with the adenoviral vector carrying firefly luciferase cDNA (Ad.luc) using different doses, times of transduction and addition of positive ions for transduction enhancement. The optimized protocol was further tested on muscle samples from 3 new patients, which were transduced with Ad.BMP-2. Released bone morphogenetic protein 2 (BMP-2) levels in osteogenic medium were measured every 3 days during the period of 21 days. Expression of osteogenic markers was at day 14 and 21. After 21 days of cultivation, muscle tissue was prepared for immunohistochemical staining for collagen type I (COL-I). </p>
Dataset for Subnormothermic ex vivo lung perfusion attenuates ischemia reperfusion injury from donation after circulatory death donors
<p>Use of normothermic <i>ex vivo</i> lung perfusion (EVLP) was adopted in clinical practice to assess the quality of marginal donor lungs. Subnormothermic perfusion temperatures are in use among other solid organs to improve biochemical, clinical and immunological parameters. In a rat EVLP model of donation after circulatory death (DCD) lung donors, we tested the effect of four subnormothermic EVLP temperatures that could further improve organ preservation. Warm ischemic time was of 2 hours. EVLP time was of 4 hours. Lung physiological data were recorded and metabolic parameters were assessed. Lung oxygenation at 21°C and 24°C were significantly improved whereas pulmonary vascular resistance and edema formation at 21°C EVLP were significantly worsened when compared to 37°C EVLP. The perfusate concentrations of potassium ions and lactate exiting the lungs with 28°C EVLP were significantly lower whereas sodium and chlorine ions with 32°C EVLP were significantly higher when compared to 37°C EVLP. Also compared to 37°C EVLP, the pro-inflammatory chemokines MIP2, MIP-1α, GRO-α, the cytokine IL-6 were significantly lower with 21°C, 24°C and 28°C EVLP, the IL-18 was significantly lower but only with 21°C EVLP and IL-1β was significantly lower at 21°C and 24°C EVLP. Compared to the 37°C EVLP, the lung tissue ATP content after 21°C, 24°C and 28°C EVLP were significantly higher, the carbonylated protein content after 28°C EVLP was significantly lower and we measured significantly higher myeloperoxidase activities in lung tissues with 21°C, 24°C and 32°C. The 28°C EVLP demonstrated acceptable physiological variables, significantly higher lung tissue ATP content and decreased tissue carbonylated proteins with reduced release of pro-inflammatory cytokines. In conclusion, the 28°C EVLP is a non inferior setting in comparison to the clinically approved 37°C EVLP and significantly improve biochemical, clinical and immunological parameters and may reduce I/R injuries of DCD lung donors.</p>
Data from: 7 Tesla MRI of the ex vivo human brain at 100 micron resolution
<p>We present an ultra-high resolution MRI dataset of an ex vivo human brain specimen. The brain specimen was donated by a 58-year-old woman who had no history of neurological disease and died of non-neurological causes. After fixation in 10% formalin, the specimen was imaged on a 7 Tesla MRI scanner at 100 µm isotropic resolution using a custom-built 31-channel receive array coil. Single-echo multi-flip Fast Low-Angle SHot (FLASH) data were acquired over 100 hours of scan time (25 hours per flip angle), allowing derivation of synthesized FLASH volumes. This dataset provides an unprecedented view of the three-dimensional neuroanatomy of the human brain. To optimize the utility of this resource, we warped the dataset into standard stereotactic space. We now distribute the dataset in both native space and stereotactic space to the academic community via multiple platforms. We envision that this dataset will have a broad range of investigational, educational, and clinical applications that will advance understanding of human brain anatomy in health and disease.</p>
Aspergillus flavus germination, ex vivo
<p>Time-lapse imaging showing intrastromal germination of Aspergillus flavus conidia following intrastromal injection into a porcine cornea. Conidial germination and hyphal elongation can be observed by sixteen hours post-inoculation. </p>
Data from: Phase-dependent differential in vitro and ex vivo susceptibility of Aspergillus flavus and Fusarium keratoplasticum to azole antifungals
<p>Fungal keratitis (FK) is an invasive infection of the cornea primarily associated with <em>Aspergillus</em> and <em>Fusarium</em> species. FK is treated empirically with a limited selection of topical antifungals with varying levels of success. Though clinical infections are typically characterized by a dense network of mature mycelium, traditional models used to test antifungal susceptibility of FK isolates exclusively evaluate susceptibility in fungal cultures derived from asexual spores known as conidia. The purpose of this study was to characterize differences in fungal response when topical antifungal treatment is initiated at progressive phases of fungal development. We compared efficacy of voriconazole and luliconazole against <em>in vitro</em> cultures of <em>A. flavus</em> and <em>F. keratoplasticum</em> at 0, 24, and 48 h of fungal development. Data from this assay is included here, reported as end-point absorbance values. A porcine cadaver corneal model was used to compare antifungal efficacy of voriconazole and luliconazole in <em>ex vivo</em> tissue cultures of <em>A. flavus</em> and <em>F. keratoplasticum</em> at 0, 24, and 48 h of fungal development. Data from this assay may be found here, reported as pixel counts of the determined area.</p>
Ex Vivo Drug Sensitivity Testing and Mutation Profiling
ClinicalTrials.gov study NCT03860376. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Injection of ex Vivo Amplified G-CSF Mobilised Autologous Peripheral Blood Stem Cell Transplantation
ClinicalTrials.gov study NCT00461955. IPD Sharing: Not stated. Countries: 1. Publications: 18.
Ex-vivo Confocal Imaging and Proteomic Profiling to Determine Treatment Response in Children With IBD
ClinicalTrials.gov study NCT07121920. IPD Sharing: NO. Countries: 1. Publications: 14.
EXplanted LIver and Ex-vivo Pancreatic Specimen Evaluation by 7 TESLA MRI
ClinicalTrials.gov study NCT04012021. IPD Sharing: NO. Countries: 1. Publications: 17.
Ex Vivo Cultured Bone Marrow Derived Allogenic MSCs in AMI
ClinicalTrials.gov study NCT00883727. IPD Sharing: Not stated. Countries: 1. Publications: 1.
The Feasibility and Safety of Normothermic ex Vivo Kidney Perfusion
ClinicalTrials.gov study NCT03136848. IPD Sharing: NO. Countries: 1. Publications: 1.
A Study Comparing ex Vivo MRI Versus Radiography of Breast Specimens
ClinicalTrials.gov study NCT01869335. IPD Sharing: Not stated. Countries: 1. Publications: 1.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.