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55 results for “immunofluorescence”
Original pictures of isolation, culture and immunofluorescence staining of primary fibroblasts from cervical tissue in vitro
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Protecting RNA Integrity for Spatial Transcriptomics While Improving Immunofluorescent Staining Quality
GEO Series GSE228891. Mus musculus. 4 samples. Type: Expression profiling by high throughput sequencing; Other.
Homophilic and heterophilic cadherin bond formation in fibroblast and epithelial cells-AFM based SCFS and immunofluorescence data
<p>This repository contains all the raw and processed data obtained from Atomic Force Microscopy based single-cell force spectroscopy and immunofluorescence microscopy for investigating the cadherin homophilic and heterophilic mechanical bond rupture forces, that are expressed in fibroblast and epithelial cell-cell adherens junctions.</p>
Antibody Validation for Cyclical Immunofluorescence of Human Kidneys (Part 2, Figure 32)
<p>This dataset includes Cyclical Immunofluorescnce (CyCIF) images (15-20 channels) of frozen human kidney sections interogated with a panel of 12 validated antibodies designed to evaluate renal tubular cell segmentation (Figures 31 and 32), an a panel of 12 validated antibodies and a lectin designed to evaluate glomerular and glomerulus-associated structures in the normal human kidney (Figure 33). We have attached an excel file (Supplemental Table 2) that includes all of the experimental and (de-identified) patient metadata associated with these images, with information and comments about each of the channel images, the antibodies used, CyCIF cycles, and the cell types and extracellular matrix compartments identified with these combinations of antibodies. Because of the sixze of the images, we have divided this into two separate datasets (Antibody Validation for Cyclical Immunofluorescence Parts 1 and 2). </p>
The datasets for Precise immunofluorescence canceling enables highly multiplexed imaging
<p>Cell states are regulated by responsive signal pathways upon ligand-binding to the receptor and inter-cellular interactions. Thus, high-resolution imaging has been attempted to explore the dynamics of signaling. Recently, multiplexed imaging has been introduced to profile cell state by acquisition of comprehensive spatial protein information in the cells. However, it is still challenging to compromise resolution for visualizing activated signals. Here we developed ‘Precise Emission Canceling Antibody (PECAb)’ attached with erasable fluorescent labeling. PECAbs allow high-resolution sequential imaging using 206 antibodies and it allowed reconstruction of the spatiotemporal dynamics of signaling pathways. Additionally, combining this approach with seq-smFISH can effectively classify cells and identify their signal activation states in human tissue. Overall, the PECAb system serves as a comprehensive platform for analyzing complex cell processes.</p>
QuantifyHER: Quantitative Immunofluorescence and/or RT-qPCR for Measuring HER2 in HER2-low Metastatic Breast Cancer
ClinicalTrials.gov study NCT06551116. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Prospective Assay for SARS-CoV-2 (COVID-19) Antibody Detection Indirectly by Immunofluorescence: SARS-CoV2 IIF Method
ClinicalTrials.gov study NCT04429620. IPD Sharing: NO. Countries: 1. Publications: 0.
Mohs and Immunofluorescence for Malignant Melanoma In Situ
ClinicalTrials.gov study NCT02306512. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Comparison of gene expression with immunofluorescent chromosome banding and ChIP-seq of histone modifications in cell-cycle sorted lymphoblastoid and HeLa cells [array]
GEO Series GSE165237. Homo sapiens. 6 samples. Type: Expression profiling by array.
Comparison of lymphoblastoid cell gene expression with immunofluorescent chromosome banding
GEO Series GSE24459. Homo sapiens. 3 samples. Type: Expression profiling by array.
Table S1: Human-specific oligonucleotide primers; Table S2: Antibodies and their final dilutions used for immunofluorescence analysis and flow cytometry.
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Single-cell RNA sequencing combined with multiplex immunofluorescence probes the role of MFAP5+ fibroblasts in the microenvironment of pancreatic ductal adenocarcinoma
GEO Series GSE285264. Homo sapiens. 3 samples. Type: Expression profiling by high throughput sequencing.
Single-cell RNA sequencing combined with multiplex immunofluorescence probes the role of MFAP5+ fibroblasts in the microenvironment of pancreatic ductal adenocarcinoma.
GEO Series GSE288067. Homo sapiens. 1 samples. Type: Other.
Appendix videos for PhD thesis: Characterisation of 3D networks and cell distributions in human lung tissue using correlative micro-CT and immunofluorescence
<p>Online repository of supplementary videos, detailed below, to compliment my PhD thesis.</p> <p><strong>A.7.1 Blood vessel segmentation with ITK-SNAP</strong></p> <p>Video showing the real time process of segmenting a blood vessel from µCT lung data using ITK-SNAP.</p> <p><strong>A.7.2. Lung 1 summary video</strong></p> <p>Video summary of the µCT data from method development tissue HL093 (Lung1) showing the 2D slices through the sectioned volume building into the 3D volume rendering. The segmentation of blood vessels (red) and Ck18 can also be seen in 2D and 3D.</p> <p><strong>A.7.3. Lung 2 summary video</strong></p> <p>Video summary of the µCT data from method development tissue HL098 (Lung2) showing the 2D slices through the sectioned volume building into the 3D volume rendering. The segmentation of blood vessels (red) and Ck18 can also be seen in 2D and 3D.</p> <p><strong>A.7.4. Non-COPD-5 Blood vessel thickness-3D</strong></p> <p>A 360° rotation of the 3D blood vessel thickness map of the Non-COPD-5 tissue seen in Figure 4.5.</p> <p><strong>A.7.5. COPD-4 Blood vessel thickness-3D</strong></p> <p>A 360° rotation of the 3D blood vessel thickness map of the COPD-4 tissue seen in Figure 4.5.</p> <p><strong>A.7.6. COPD-4 Full segmentation of sectioned volume</strong></p> <p>A 360° rotation of the full segmentation seen in Figure 5.18. The µCT volume has been made semi-transparent to visualise the segmentation of blood vessels (red), Ck18 (blue), dilated AA1-mast cells (pink) and dilated CD68-macrophages (yellow).</p>
Tiled Hematoxylin and Eosin and Multiplex Immunofluorescence Slides for Translation
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.