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FIGURE 6 in Re-description of Craspodema reflectans (Nematoda, Cyatholaimidae) using confocal laser scanning microscopy

FIGURE 6. Light micrographs of Craspodema octogoniata male. A) Detail of the amphideal fovea; B) Detail of the dorsal tooth; C) View of the ventral teeth; D) Detail of the cuticular ornamentation in the middle of the body; E) Precloacal supplements; F) Detail of the copulatory apparatus. Scale bars: A–C = 5µm; D = 10µm; E,F = 10µm.

FIGURE 3 in Re-description of Craspodema reflectans (Nematoda, Cyatholaimidae) using confocal laser scanning microscopy

FIGURE 3. Female specimen of Craspodema reflectans. A) Habitus; B) Detail of the amphideal fovea; C) Detail of the buccal cavity. Scale bars: A = 100µm; B,C = 10 µm.

FIGURE 7 in Re-description of Craspodema reflectans (Nematoda, Cyatholaimidae) using confocal laser scanning microscopy

FIGURE 7. Confocal laser scanning micrograph of Craspodema octogoniata male. A) Detail of the amphideal fovea; B) Reconstruction of the cephalic region of a male and detail of the rugae; C) Reconstruction of the buccal cavity; D) Reconstruction of the copulatory apparatus. Scale bars: A–D = 5 µm.

FIGURE 2 in Re-description of Craspodema reflectans (Nematoda, Cyatholaimidae) using confocal laser scanning microscopy

FIGURE 2. Light micrographs of a male of Craspodema reflectans. A) Detail of the buccal cavity; B) Detail of the amphideal fovea; C–D) cuticular ornamentation of the middle part of the body and cervical region; E) Precloacal supplements and gubernaculum structure; F) Detail of the copulatory apparatus. Scale bars: A = 5µm; B–F = 10 µm.

FIGURE 5 in Re-description of Craspodema reflectans (Nematoda, Cyatholaimidae) using confocal laser scanning microscopy

FIGURE 5. Confocal laser scanning micrograph of Craspodema reflectans. A) Reconstruction of the cephalic region of a male and detail of the eversible rugae; B) Reconstruction of the buccal cavity of a male; C) View of the male amphideal fovea; D) View of the female amphideal fovea; E) View of the precloacal supplements region; F) Reconstruction of the copulatory apparatus. Scale bars: A = 5 µm; B–F = 10 µm.

FIGURE 6. 3D in Confocal laser scanning microscopy technique for the study of internal genitalia and external morphology of eriophyoid mites (Acari: Eriophyoidea)

FIGURE 6. 3D-reconstruction of the internal genitalia of Phytoptus rigidus, bottom view (the same female as Fig. 2A). A. spermathecae, B. spherical spermathecal tube, C. capsule-like reservoir in the connection of two spermathecal tubes, D. transversal genital apodeme, E. distal folder of transversal genital apodeme, F. longitudinal genital? apodeme1 (includes two sclerotised plates), G. epigynium.

FIGURE 8 in Confocal laser scanning microscopy technique for the study of internal genitalia and external morphology of eriophyoid mites (Acari: Eriophyoidea)

FIGURE 8. CLSM images of eriophyoid mites using reflected red laser (wavelength emission range of 627–637 nm). A. empodia and leg setae of Phytoptus?tetratrichus; B. empodia and leg setae of P. atherodes; C. gnathosomal stylets of Phytoptus chamaebatiae; D. antero-ventral aspect of Setoptus pini; E. antero-ventral aspect of Oziella sp.; F. prodorsal shield of Boczekella reticulata. Note: the colour was changed from red (original pseudo-colour) to white (A, C, E, F) and to light blue (B) to increase contrast.

FIGURE 3 in Confocal laser scanning microscopy technique for the study of internal genitalia and external morphology of eriophyoid mites (Acari: Eriophyoidea)

FIGURE 3. Images obtained via LSCM of specimens on older microscope slides. A. Boczekella reticulata (slide-mounted on 11 May 2010); B. Sierraphytoptus ambulans (21 Sept. 2008), C. Phytoptus atherodes (5 Aug. 2003), D. Novophytoptus?stipae (9–14 Sept. 1976, uncoloured and black-white inverted). Note: empodia and proximal part of setae are not visible with blue laser (see 2B & 2D).

FIGURE 5 in Confocal laser scanning microscopy technique for the study of internal genitalia and external morphology of eriophyoid mites (Acari: Eriophyoidea)

FIGURE 5. CLSM images (blue laser) of female internal genitalia (A) and spermatophore inside male (B). A. Phytoptus chamaebatiae. B. Setoptus pini. Note: original pseudocolour obtained from CLS microscope using Leica software was saved on both images.

FIGURE 2 in Confocal laser scanning microscopy technique for the study of internal genitalia and external morphology of eriophyoid mites (Acari: Eriophyoidea)

FIGURE 2. Larvae of Phytoptus hirtae inside egg (fresh microscope slides). Note: the colour was changed from original (pseudo colour) red to light blue-green for enhancing contrast.

FIGURE 1 in Confocal laser scanning microscopy technique for the study of internal genitalia and external morphology of eriophyoid mites (Acari: Eriophyoidea)

FIGURE 1. Images obtained via CLSM of fresh microscope slides (specimens mounted between April and September 2011). A. Phytoptus rigidus; B. Trisetacus?kirghisorum; C. Setoptus pini. Original colour (pseudo-colour) kept. Note: blue is the colour of the exciting laser.

FIGURE 7 in Confocal laser scanning microscopy technique for the study of internal genitalia and external morphology of eriophyoid mites (Acari: Eriophyoidea)

FIGURE 7. CLSM images of empodia of Setoptus pini (reflected laser light): A. red laser, B. blue laser, C. green laser. Note: the original pseudo-colour was changed to red (A), blue (B) and green (C).

opennotspecifiedMar 2021View details →

FIGURE 1 in Micromorphology of seeds of three Mexican species of Pinguicula (Lentibulariaceae) show autofluorescence using confocal laser scanning microscopy

FIGURE 1. Confocal laser scanning microscopy images showing the micromorphological features of Pinguicula seeds. A–D. Pinguicula casperi; E–H. P. parvifolia; I–L. P. oblongiloba. A, E and I are a general view of the entire seeds (20×, scale bar 100 μm); B, F and J show the micropylar end (60×, scale bar 20 μm); D, H, and L show the chalazal end of the seed (60×, scale bar 20 μm); C, G and K show the surface details of the seeds (100×, scale bar 10 μm).

opencc-by-4.0Apr 2022View details →

Dynamic deformation calculation of articular cartilage and cells using resonance-driven laser scanning microscopy - Osmotic Challenge and Validation Testing Dataset

<p>This archive contains 3-D image stacks obtained over time of articular cartilage undergoing osmotic swelling. These were acquired with a resonance scanning protocol, which allows for fast scanning but with decreased image quality. The Python package, resonant_lsm, was developed to segment and analyze the deformation of cells in such images. The archive also contains validation testing data of this software. Included README files document the archive contents and how to reproduce the analyses. </p>

opennotspecifiedDec 2017View details →

Fig. 2 Confocal laser scanning microscopy images showing the hard tick morphology. a in parasitised feathered dinosaurs as Cretaceous amber assemblages revealed

Fig. 2 Confocal laser scanning microscopy images showing the hard tick morphology. a Habitus in ventral view of the Cornupalpatum burmanicum nymph associated with feathers. Scale bar, 0.2 mm. b Detail of the gnathosoma and coxal area in ventral view revealing the absence of genital pore. Scale bar, 0.1 mm. c Dorsal view detail of the gnathosoma and anterior part of the scutum (arrow indicates the lateral margin of the scutum). Scale bar, 0.1 mm

Figure 3 in Confocal laser scanning microscopy analysis of the phalloidin-labelled musculature in nemerteans

Figure 3. Confocal projections of the longitudinal sections of the body wall musculature showing diagonal muscles (A–E) and fascias of the extracellular matrix (F, arrows), dorsal view. (A) Carinina sp., precerebral region; (B) Hubrechtella juliae, just behind mouth; (C) Cephalothrix cf. simula, just behind mouth; (D) Tetrastemma sp., middle body region; (E) Quasitetrastemma stimpsoni, middle body region; (F) Collarenemertes bimaculata, middle body region. Abbreviation: gp, gonopore. Scales: 50 µm.

Figure 5 in Confocal laser scanning microscopy analysis of the phalloidin-labelled musculature in nemerteans

Figure 5. Confocal projections of the transversal (A, B) and longitudinal (C–I) sections of the proboscis. Anterior (A–D, F, G) and middle (E, H, I) proboscis portions. (A, B), everted proboscis of Cerebratulus marginatus; (C) third proboscis region of Callinera sp.; (D) longitudinal and diagonal musculature in region of muscle cross (arrows) of Nipponomicrura uchidai; (E) bulb region of Emplectonema gracile; (F, G) anterior proboscis region of Ototyphlonemertes martynovi (F) and Ototyphlonemertes valentinae (G); (H, I) middle proboscis region of Ototyphlonemertes martynovi (H) and Ototyphlonemertes valentinae (I). Abbreviations: as, anterior sphincter; cm, circular musculature; dm, diagonal musculature; ge, glandular epithelium; icm, inner circular musculature; lm, longitudinal musculature; ls, longitudinal muscle strand; mc, muscle cross; pms, posterior muscle sheath. Scale: (A), 200 µm; (B–E, G), 50 µm; (F, H, I), 20 µm.

Figure 7 in Confocal laser scanning microscopy analysis of the phalloidin-labelled musculature in nemerteans

Figure 7. Confocal projections of the longitudinal sections of gonadal sac musculature. (A, B, E, F) mature ovaries of Emplectonema gracile (A), Antarctonemertes varvarae (B), Tetrastemma sp. (E), and Malacobdella grossa (F), ventral view; (C) immature testis of Antarctonemertes varvarae, ventral view; (D) gonopore of Tetrastemma sp., dorsal view. Abbreviation: gp, gonopore; lm, longitudinal body musculature. Scale: (A–E), 50 µm; (F), 100 µm.

Figure 2 in Confocal laser scanning microscopy analysis of the phalloidin-labelled musculature in nemerteans

Figure 2. Confocal projections of the longitudinal (A–D) and transversal (E) sections showing dermal musculature. (A, B) Cerebratulus marginatus, just behind mouth, dorsal view (A), precerebral region, dorsal view (B); (C) Lineus alborostratus, just behind mouth, dorsal view; (D, E) Hubrechtella juliae, brain region, dorsal view (D), transversal section through the brain region showing connection of the dermal and outer circular muscles (arrows) (E). Abbreviations: br, brain; cdm, circular dermal muscles; em, epidermal muscle processes; lm, longitudinal musculature; pr, proboscis. Scales: 50 µm.

Figure 6 in Confocal laser scanning microscopy analysis of the phalloidin-labelled musculature in nemerteans

Figure 6. Schematic diagrams of the body wall musculature (A–C) and proboscis musculature (D–F). (A) Tubulanid palaeonemerteans; (B) heteronemerteans; (C) hoplonemerteans; (D, D̓) palaeonemerteans (D, Cephalothrix; D̓, Carinoma); (E) Ototyphlonemertes valentinae; (F) lineid nemerteans (only outer circular and diagonal musculature). Abbreviations: ddm, diagonal dermal muscles; dm, diagonal muscles; icm, inner circular musculature; ilm, inner longitudinal musculature; ldm, longitudinal dermal muscles; lm, longitudinal musculature; ls, longitudinal muscle strand; mc, muscle cross; mcm, middle circular musculature; ocm, outer circular musculature; olm, outer longitudinal musculature; rem, radial epidermal muscles.