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105 results for “molecular sexing”
FIGURE 11 in A revision of Miobantia Giglio-Tos, 1917 (Mantodea: Thespidae, Miobantiinae), with molecular association of dimorphic sexes and immature stages
FIGURE 11. Miobantia, head from the frontal perspective of the frontal sclerite. A, M. aptera Giglio-Tos, 1917, female holotype; B, M. ciliata (Stål, 1860), male MT00047; C, M. fuscata (Giglio-Tos, 1915), male MT00030; D, M. rustica (Fabricius, 1781), male holotype of junior synonym M. nebulosa of Giglio-Tos; E, M. immanis n. sp., male holotype; F, M. arctissima n. sp., male holotype; G, M. sulista n. sp., male holotype; H, M. nordestina n. sp., male holotype.
FIGURE 12 in A revision of Miobantia Giglio-Tos, 1917 (Mantodea: Thespidae, Miobantiinae), with molecular association of dimorphic sexes and immature stages
FIGURE 12. Miobantia, head from the frontal perspective of the ocelli. M. aptera Giglio-Tos, 1917: A, male MT00087; B, female holotype. M. ciliata (Stål, 1860): C, male MT00047; D, female collected in 26.XI-02.XII.2006. M. fuscata (Giglio-Tos, 1915): E, male MT00030; F, female from Reserva Natural Vale, collected in 13.I.2011. M. rustica (Fabricius, 1781): G, male holotype of junior synonym M. nebulosa of Giglio-Tos. M. arctissima n. sp.: H, male holotype.
FIGURE 27 in A revision of Miobantia Giglio-Tos, 1917 (Mantodea: Thespidae, Miobantiinae), with molecular association of dimorphic sexes and immature stages
FIGURE 27. Distribution maps for species of Miobantia with new records provided in the text and localities found on literature.
FIGURE 9. Miobantia sulista n in A revision of Miobantia Giglio-Tos, 1917 (Mantodea: Thespidae, Miobantiinae), with molecular association of dimorphic sexes and immature stages
FIGURE 9. Miobantia sulista n. sp., dorsal habitus. A, male holotype; B, female allotype, paratype of Giglio-Tos' description of M. aptera; C, allotype label.
FIGURE 7. Miobantia immanis n in A revision of Miobantia Giglio-Tos, 1917 (Mantodea: Thespidae, Miobantiinae), with molecular association of dimorphic sexes and immature stages
FIGURE 7. Miobantia immanis n. sp., dorsal habitus. A, male holotype; B, male MT00045; C, female allotype.
FIGURE 6 in A revision of Miobantia Giglio-Tos, 1917 (Mantodea: Thespidae, Miobantiinae), with molecular association of dimorphic sexes and immature stages
FIGURE 6. Miobantia rustica (Fabricius, 1781), dorsal habitus. A, male holotype of junior synonym M. nebulosa of Giglio- Tos; B, label of the holotype of M. nebulosa (not to scale); C, male lectotype (G. Svenson phot., Copyright of The Natural History Museum, London); D, lectotype label (G. Svenson phot., Copyright of The Natural History Museum, London; not to scale).
FIGURE 5 in A revision of Miobantia Giglio-Tos, 1917 (Mantodea: Thespidae, Miobantiinae), with molecular association of dimorphic sexes and immature stages
FIGURE 5. Miobantia phryganea (Saussure, 1869), dorsal habitus, male syntype (P. Schwendinger phot.).
FIGURE 4 in A revision of Miobantia Giglio-Tos, 1917 (Mantodea: Thespidae, Miobantiinae), with molecular association of dimorphic sexes and immature stages
FIGURE 4. Miobantia fuscata (Giglio-Tos, 1915), dorsal habitus. A, male MT00030; B, male holotype; C, holotype label (not to scale); D, female from Reserva Natural Vale, collected in 13.I.2011; E, male MT00013; F, male MT00070.
FIGURE 2 in A revision of Miobantia Giglio-Tos, 1917 (Mantodea: Thespidae, Miobantiinae), with molecular association of dimorphic sexes and immature stages
FIGURE 2. Miobantia aptera Giglio-Tos, 1917, dorsal habitus. A, male MT00051; B, female from Bicuíba, Reserva Natural Vale; C, holotype label (not to scale); D, female holotype.
FIGURE 1 in A revision of Miobantia Giglio-Tos, 1917 (Mantodea: Thespidae, Miobantiinae), with molecular association of dimorphic sexes and immature stages
FIGURE 1. Strict consensus tree of relationships between specimens of Miobantia resulted in the cladistic analysis using implied weighting (k = 1). The numbers on branches are the supports of the clades measured with symmetrical resampling method, showed as frequency differences (%). Clades with support below 50% are collapsed. The results of delimitation and identification of species is provided in the right side of vertical bars.
FIGURE 3 in A revision of Miobantia Giglio-Tos, 1917 (Mantodea: Thespidae, Miobantiinae), with molecular association of dimorphic sexes and immature stages
FIGURE 3. Miobantia ciliata (Stål, 1860), dorsal habitus. A, male MT00047; B, female collected in 26.XI-02.XII.2006; C, male holotype (G. Lindberg phot.); D, male MT00033; E, holotype label (not to scale).
Low-coverage whole-genome sequencing reveals molecular markers for spawning season and sex identification in Gulf of Maine Atlantic cod (Gadus morhua, Linnaeus 1758)
<p class="CxSpFirst">Atlantic cod (<i>Gadus morhua</i>,<i> </i>Linnaeus 1758) in the western Gulf of Maine are managed as a single stock despite several lines of evidence supporting two spawning groups (spring and winter) that overlap spatially, while exhibiting seasonal spawning isolation. Low-coverage whole genome sequencing was used to evaluate the genomic population structure of Atlantic cod spawning groups in the western Gulf of Maine and Georges Bank using 222 individuals collected over multiple years. Results indicated low total genomic differentiation, while also showing strong differentiation between spring and winter spawning groups at specific regions of the genome. Guided regularized random forest and ranked <i>F</i><sub>ST</sub> methods were used to select panels of single nucleotide polymorphisms (SNPs) that could reliably distinguish spring and winter-spawning Atlantic cod (88.5% assignment rate), as well as males and females (95.0% assignment rate) collected in the western Gulf of Maine. These SNP panels represent a valuable tool for fisheries research and management of Atlantic cod in the western Gulf of Maine that will aid investigations of stock production and support accuracy of future assessments.</p>
Mapping and assembly of the Midas cichlid male-specific region supports molecular parallelism in the evolution of a master sex-determining role for amhr2
<p>The evolution of sex chromosomes and their differentiation from autosomes is a major event during genome evolution that happened many times in several lineages. The repeated evolution and lability of sex-determination mechanisms in fishes makes this a well-suited system to test for general and predictable patterns in evolution. According to current theory, differentiation is triggered by the suppression of recombination following the evolution of a new master-sex determining gene. However, the molecular mechanisms that establish recombination suppression are known from few examples, owing to the intrinsic difficulties of assembling sex determining regions (SDRs). Forward-genetics data and the development of long-read sequencing have generated a wealth of data questioning central aspects of the current theory. Here, we demonstrate that sex in Midas cichlids is determined by an XY system, identify and assemble the SDR by combining forward-genetics, long-read sequencing and optical mapping. We show how long-reads aid in the detection of artifacts in genotype-phenotype mapping that arise from incomplete genome assemblies. The male-specific region is restricted to a 100 kb segment on chromosome 4 that harbors transposable elements and a Y-specific duplicate of the anti-Mullerian receptor 2 locus, a known sex-determining gene. Our data suggests that <em>amhr2Y</em> originated by an interchromosomal translocation from chromosome 20 to 4 predating the split of Midas and Flier cichlids. In the later, it is pseudogenized and translocated to another chromosome. Duplication of anti-Mullerian genes is a common route to establishing new sex determiners, highlighting the role of molecular parallelism in the evolution of sex determination.</p>
FIGURE 5 in Sarcophaga (Hoa) flexuosa Ho (Diptera: Sarcophagidae): association of sexes using morphological and molecular approaches, and a redefinition of Hoa Rohdendorf
FIGURE 5. Sarcophaga (Hoa) flexuosa Ho, 1934. A. Male sternite 5. Scale = 0.50mm. B. Female terminalia, posteroventral view. Scale = 0.25mm. C. Female sternites 1 to 4, ventral view. Scale = 0.50mm.
FIGURE 4 in Sarcophaga (Hoa) flexuosa Ho (Diptera: Sarcophagidae): association of sexes using morphological and molecular approaches, and a redefinition of Hoa Rohdendorf
FIGURE 4. Sarcophaga (Hoa) flexuosa Ho, 1934. A. Distiphallus, anterior. Scale = 200µm. B. Median stylus and vesica. Scale = 100µm. C. Median stylus, anterior. Scale = 25µm. D. Vesica. Scale = 25µm. E. Basal part of vesica. Scale = 15µm. F. Distiphallus, lateral. Scale = 100µm. G. Lateral stylus. Scale = 50µm. Abbreviations: H, harpes; J, juxta; JE, juxtal extension; LS, lateral stylus; MS, median stylus; PT, phallic tube; V, vesica.
FIGURE 3 in Sarcophaga (Hoa) flexuosa Ho (Diptera: Sarcophagidae): association of sexes using morphological and molecular approaches, and a redefinition of Hoa Rohdendorf
FIGURE 3. Male terminalia, lateral view. Sarcophaga basiseta Baranov, 1931 (adapted from Baranov, 1931). B. Sarcophaga (Hoa) flexuosa Ho, 1934.
FIGURE 2 in Sarcophaga (Hoa) flexuosa Ho (Diptera: Sarcophagidae): association of sexes using morphological and molecular approaches, and a redefinition of Hoa Rohdendorf
FIGURE 2. Sarcophaga (Hoa) flexuosa Ho, 1934. A. Male head, anterior view. B. Female head, anterior view. C. Male head, left anterolateral view. D. Female head, left anterolateral view. E. Male abdomen, dorsal view. F. Female abdomen, dorsal view. Scales for A–D = 0.50mm. Scales for E & F = 1.00mm.
FIGURE 1 in Sarcophaga (Hoa) flexuosa Ho (Diptera: Sarcophagidae): association of sexes using morphological and molecular approaches, and a redefinition of Hoa Rohdendorf
FIGURE 1. Sarcophaga (Hoa) flexuosa Ho, 1934. A. Male body, lateral view. B. Female body, lateral view. Scales = 2.00mm.
Low-coverage whole-genome sequencing reveals molecular markers for spawning season and sex identification in Gulf of Maine Atlantic cod (Gadus morhua, Linnaeus 1758)
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Mapping and assembly of the Midas cichlid male-specific region supports molecular parallelism in the evolution of a master sex-determining role for amhr2
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