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zenodo40/100

Ditrect STORM imaging and image reconstruction of the transcription initiation marker P-S5 and nuclear PI(4,5)P2 indirectly immunolabeled with AF647 (red) and AF555 (green) in THZ1 treated cells.

U-2 OS cells were grown in DMEM with 10% FBS at 37°C and 5% CO2. Cells were plated one day before staining in ~50% confluence on the high-precision 12 mm round coverslips treated with Hellmanex, sonicated, washed, dried and sterilized. Cells were treated for 3h with 1 µM THZ1 (MedChem HY80013) added to the culture media. U2OS cells were washed twice with PBS (pH 7.4) and fixed for 30 min in 2% PFA in PBS, washed 3-times for 5 min with PBS, then permeabilized in 0.1% Triton X-100 in PBS for 20 min, washed 3-times for 5 min by PBS and blocked in filtered 5% BSA in PBS for 30 min. Cells were incubated for 45 min with rabbit polyclonal IgG anti-RNAPII CTD P-S5 (Abcam ab5131) 3 µg/mL and mouse ascites IgM anti-PI(4,5)P2 2C11 (Z-A045; Echelon Biosci. Inc., USA) 5 µg/mL  in 5% BSA in PBS, washed 3-times for 5 min in PBS and incubated for 30 min with goat anti-mouse IgM (µ-chain) AF555 (Jackson ImmunoRes. A24126) 10 µg/mL; goat anti-rabbit IgG AF647 (Invitrogen A21245) 10 µg/mL diluted in 5% BSA in PBS. Then the cells were washed 3-times for 5 min in PBS, post-fixed for 15 min in 2% PFA in PBS and washed 3-times for 5 min in PBS. All procedures were performed at RT and the cells were stored in PBS in the fridge overnight prior imaging. Coverslips with cells were mounted in the Chamlide chamber (Live Cell Instrument, Korea) and covered with imaging buffer (PBS pH 7.4, 50 mM MEA). Single-molecule localizations (SMLs) data were acquired by Zeiss Elyra PS.1 equipped with HR Diode 642-150 and HR DPSS 561-200 lasers, Alpha Plan-Apochromat 100x/1.46 oil DIC M27 Elyra objective and Andor EM CCD iXon DU 897 camera and Zeiss ZEN Black 2.1 SP3 software (Zeiss). AF647 and AF555 photo-switching was achieved by HiLo illumination and TIRF HP FOV with 100% power of 642nm or 561nm laser, and the signal was acquired via MBS 642 + EF LP 655 and MBS 561 + EF BP 570-620 / LP 750 filters, respectively. Exposure time was 40 ms and EM gain was 300 for both channels. SMLs were calculated in 2D by Zeiss ZEN Black 2.1 SP3 software using x,y 2D Gauss fit with point spread function (PSF) half width 177.9 nm, peak mask size 9 pixels and peak intensity to noise 6 and accounted for overlap in 2D with max cluster size 10. SMLs were rendered in ZEN software with 10 nm/px resolution and 1x PSF expansion factor. The data were model-based drift corrected in ZEN. Two channels were aligned using tetraspec beads fiducial markers for affine calibration. Drift-corrected and aligned localization coordinates were exported as text files. Text files were converted into csv files and imported using self-written macro (Hoboth et al., 2021a) into the ImageJ2 (Rueden et al., 2017) plug-in ThunderSTORM, visualized by normalized Gaussian method (Ovesny et al., 2014).

opencc-by-4.0Apr 2024View details →

zenodo40/100

Ditrect STORM imaging and image reconstruction of the transcription initiation marker P-S5 and nuclear PI(3,4)P2 indirectly immunolabeled with AF647 (red) and AF555 (green) in THZ1 treated cells.

U-2 OS cells were grown in DMEM with 10% FBS at 37°C and 5% CO2. Cells were plated one day before staining in ~50% confluence on the high-precision 12 mm round coverslips treated with Hellmanex, sonicated, washed, dried and sterilized. Cells were treated for 3h with 1 µM THZ1 (MedChem HY80013) added to the culture media. U2OS cells were washed twice with PBS (pH 7.4) and fixed for 30 min in 2% PFA in PBS, washed 3-times for 5 min with PBS, then permeabilized in 0.1% Triton X-100 in PBS for 20 min, washed 3-times for 5 min by PBS and blocked in filtered 5% BSA in PBS for 30 min. Cells were incubated for 45 min with rabbit polyclonal IgG anti-RNAPII CTD P-S5 (Abcam ab5131) 3 µg/mL and mouse monoclonal IgG2 anti-PI(3,4)P2 (Z-P034; Echelon Biosci. Inc., USA) 5 µg/mL  in 5% BSA in PBS, washed 3-times for 5 min in PBS and incubated for 30 min with donkey anti-mouse IgG AF555 (Invitrogen A31570) 10 µg/mL and goat anti-rabbit IgG AF647 (Invitrogen A21245) 10 µg/mL diluted in 5% BSA in PBS. Then the cells were washed 3-times for 5 min in PBS, post-fixed for 15 min in 2% PFA in PBS and washed 3-times for 5 min in PBS. All procedures were performed at RT and the cells were stored in PBS in the fridge overnight prior imaging. Coverslips with cells were mounted in the Chamlide chamber (Live Cell Instrument, Korea) and covered with imaging buffer (PBS pH 7.4, 50 mM MEA). Single-molecule localizations (SMLs) data were acquired by Zeiss Elyra PS.1 equipped with HR Diode 642-150 and HR DPSS 561-200 lasers, Alpha Plan-Apochromat 100x/1.46 oil DIC M27 Elyra objective and Andor EM CCD iXon DU 897 camera and Zeiss ZEN Black 2.1 SP3 software (Zeiss). AF647 and AF555 photo-switching was achieved by HiLo illumination and TIRF HP FOV with 100% power of 642nm or 561nm laser, and the signal was acquired via MBS 642 + EF LP 655 and MBS 561 + EF BP 570-620 / LP 750 filters, respectively. Exposure time was 40 ms and EM gain was 300 for both channels. SMLs were calculated in 2D by Zeiss ZEN Black 2.1 SP3 software using x,y 2D Gauss fit with point spread function (PSF) half width 177.9 nm, peak mask size 9 pixels and peak intensity to noise 6 and accounted for overlap in 2D with max cluster size 10. SMLs were rendered in ZEN software with 10 nm/px resolution and 1x PSF expansion factor. The data were model-based drift corrected in ZEN. Two channels were aligned using tetraspec beads fiducial markers for affine calibration. Drift-corrected and aligned localization coordinates were exported as text files. Text files were converted into csv files and imported using self-written macro (Hoboth et al., 2021a) into the ImageJ2 (Rueden et al., 2017) plug-in ThunderSTORM, visualized by normalized Gaussian method (Ovesny et al., 2014).

opencc-by-4.0Apr 2024View details →

zenodo40/100

Ditrect STORM imaging and image reconstruction of the transcription initiation marker P-S5 and nuclear PI(3,4)P2 indirectly immunolabeled with AF647 (red) and AF555 (green) in DRB treated cells.

U-2 OS cells were grown in DMEM with 10% FBS at 37°C and 5% CO2. Cells were plated one day before staining in ~50% confluence on the high-precision 12 mm round coverslips treated with Hellmanex, sonicated, washed, dried and sterilized. Cells were treated for 2h with 100 µM DRB (Sigma D1916) added to the cell culture media. U2OS cells were washed twice with PBS (pH 7.4) and fixed for 30 min in 2% PFA in PBS, washed 3-times for 5 min with PBS, then permeabilized in 0.1% Triton X-100 in PBS for 20 min, washed 3-times for 5 min by PBS and blocked in filtered 5% BSA in PBS for 30 min. Cells were incubated for 45 min with rabbit polyclonal IgG anti-RNAPII CTD P-S5 (Abcam ab5131) 3 µg/mL and mouse monoclonal IgG2 anti-PI(3,4)P2 (Z-P034; Echelon Biosci. Inc., USA) 5 µg/mL  in 5% BSA in PBS, washed 3-times for 5 min in PBS and incubated for 30 min with donkey anti-mouse IgG AF555 (Invitrogen A31570) 10 µg/mL and goat anti-rabbit IgG AF647 (Invitrogen A21245) 10 µg/mL diluted in 5% BSA in PBS. Then the cells were washed 3-times for 5 min in PBS, post-fixed for 15 min in 2% PFA in PBS and washed 3-times for 5 min in PBS. All procedures were performed at RT and the cells were stored in PBS in the fridge overnight prior imaging. Coverslips with cells were mounted in the Chamlide chamber (Live Cell Instrument, Korea) and covered with imaging buffer (PBS pH 7.4, 50 mM MEA). Single-molecule localizations (SMLs) data were acquired by Zeiss Elyra PS.1 equipped with HR Diode 642-150 and HR DPSS 561-200 lasers, Alpha Plan-Apochromat 100x/1.46 oil DIC M27 Elyra objective and Andor EM CCD iXon DU 897 camera and Zeiss ZEN Black 2.1 SP3 software (Zeiss). AF647 and AF555 photo-switching was achieved by HiLo illumination and TIRF HP FOV with 100% power of 642nm or 561nm laser, and the signal was acquired via MBS 642 + EF LP 655 and MBS 561 + EF BP 570-620 / LP 750 filters, respectively. Exposure time was 40 ms and EM gain was 300 for both channels. SMLs were calculated in 2D by Zeiss ZEN Black 2.1 SP3 software using x,y 2D Gauss fit with point spread function (PSF) half width 177.9 nm, peak mask size 9 pixels and peak intensity to noise 6 and accounted for overlap in 2D with max cluster size 10. SMLs were rendered in ZEN software with 10 nm/px resolution and 1x PSF expansion factor. The data were model-based drift corrected in ZEN. Two channels were aligned using tetraspec beads fiducial markers for affine calibration. Drift-corrected and aligned localization coordinates were exported as text files. Text files were converted into csv files and imported using self-written macro (Hoboth et al., 2021a) into the ImageJ2 (Rueden et al., 2017) plug-in ThunderSTORM, visualized by normalized Gaussian method (Ovesny et al., 2014).

opencc-by-4.0Apr 2024View details →

zenodo40/100

Ditrect STORM imaging and image reconstruction of the transcription elongation marker P-S2 and nuclear PI(4,5)P2 indirectly immunolabeled with AF647 (red) and AF555 (green) in control cells.

U-2 OS cells were grown in DMEM with 10% FBS at 37°C and 5% CO2. Cells were plated one day before staining in ~50% confluence on the high-precision 12 mm round coverslips treated with Hellmanex, sonicated, washed, dried and sterilized. Cells were control treated with 1:000 DMSO in the culture media. U2OS cells were washed twice with PBS (pH 7.4) and fixed for 30 min in 2% PFA in PBS, washed 3-times for 5 min with PBS, then permeabilized in 0.1% Triton X-100 in PBS for 20 min, washed 3-times for 5 min by PBS and blocked in filtered 5% BSA in PBS for 30 min. Cells were incubated for 45 min with rabbit polyclonal IgG anti-RNAPII CTD P-S2 (Abcam ab5095) 3 µg/mL and mouse ascites IgM anti-PI(4,5)P2 2C11 (Z-A045; Echelon Biosci. Inc., USA) 5 µg/mL  in 5% BSA in PBS, washed 3-times for 5 min in PBS and incubated for 30 min with goat anti-mouse IgM (µ-chain) AF555 (Jackson ImmunoRes. A24126) 10 µg/mL; goat anti-rabbit IgG AF647 (Invitrogen A21245) 10 µg/mL diluted in 5% BSA in PBS. Then the cells were washed 3-times for 5 min in PBS, post-fixed for 15 min in 2% PFA in PBS and washed 3-times for 5 min in PBS. All procedures were performed at RT and the cells were stored in PBS in the fridge overnight prior imaging. Coverslips with cells were mounted in the Chamlide chamber (Live Cell Instrument, Korea) and covered with imaging buffer (PBS pH 7.4, 50 mM MEA). Single-molecule localizations (SMLs) data were acquired by Zeiss Elyra PS.1 equipped with HR Diode 642-150 and HR DPSS 561-200 lasers, Alpha Plan-Apochromat 100x/1.46 oil DIC M27 Elyra objective and Andor EM CCD iXon DU 897 camera and Zeiss ZEN Black 2.1 SP3 software (Zeiss). AF647 and AF555 photo-switching was achieved by HiLo illumination and TIRF HP FOV with 100% power of 642nm or 561nm laser, and the signal was acquired via MBS 642 + EF LP 655 and MBS 561 + EF BP 570-620 / LP 750 filters, respectively. Exposure time was 40 ms and EM gain was 300 for both channels. SMLs were calculated in 2D by Zeiss ZEN Black 2.1 SP3 software using x,y 2D Gauss fit with point spread function (PSF) half width 177.9 nm, peak mask size 9 pixels and peak intensity to noise 6 and accounted for overlap in 2D with max cluster size 10. SMLs were rendered in ZEN software with 10 nm/px resolution and 1x PSF expansion factor. The data were model-based drift corrected in ZEN. Two channels were aligned using tetraspec beads fiducial markers for affine calibration. Drift-corrected and aligned localization coordinates were exported as text files. Text files were converted into csv files and imported using self-written macro (Hoboth et al., 2021a) into the ImageJ2 (Rueden et al., 2017) plug-in ThunderSTORM, visualized by normalized Gaussian method (Ovesny et al., 2014).

opencc-by-4.0Apr 2024View details →

zenodo40/100

Ditrect STORM imaging and image reconstruction of the transcription elongation marker P-S2 and nuclear PI(4,5)P2 indirectly immunolabeled with AF647 (red) and AF555 (green) in DRB treated cells.

U-2 OS cells were grown in DMEM with 10% FBS at 37°C and 5% CO2. Cells were plated one day before staining in ~50% confluence on the high-precision 12 mm round coverslips treated with Hellmanex, sonicated, washed, dried and sterilized. Cells were treated for 2h with 100 µM DRB (Sigma D1916) added to the cell culture media. U2OS cells were washed twice with PBS (pH 7.4) and fixed for 30 min in 2% PFA in PBS, washed 3-times for 5 min with PBS, then permeabilized in 0.1% Triton X-100 in PBS for 20 min, washed 3-times for 5 min by PBS and blocked in filtered 5% BSA in PBS for 30 min. Cells were incubated for 45 min with rabbit polyclonal IgG anti-RNAPII CTD P-S2 (Abcam ab5095) 3 µg/mL and mouse ascites IgM anti-PI(4,5)P2 2C11 (Z-A045; Echelon Biosci. Inc., USA) 5 µg/mL  in 5% BSA in PBS, washed 3-times for 5 min in PBS and incubated for 30 min with goat anti-mouse IgM (µ-chain) AF555 (Jackson ImmunoRes. A24126) 10 µg/mL; goat anti-rabbit IgG AF647 (Invitrogen A21245) 10 µg/mL diluted in 5% BSA in PBS. Then the cells were washed 3-times for 5 min in PBS, post-fixed for 15 min in 2% PFA in PBS and washed 3-times for 5 min in PBS. All procedures were performed at RT and the cells were stored in PBS in the fridge overnight prior imaging. Coverslips with cells were mounted in the Chamlide chamber (Live Cell Instrument, Korea) and covered with imaging buffer (PBS pH 7.4, 50 mM MEA). Single-molecule localizations (SMLs) data were acquired by Zeiss Elyra PS.1 equipped with HR Diode 642-150 and HR DPSS 561-200 lasers, Alpha Plan-Apochromat 100x/1.46 oil DIC M27 Elyra objective and Andor EM CCD iXon DU 897 camera and Zeiss ZEN Black 2.1 SP3 software (Zeiss). AF647 and AF555 photo-switching was achieved by HiLo illumination and TIRF HP FOV with 100% power of 642nm or 561nm laser, and the signal was acquired via MBS 642 + EF LP 655 and MBS 561 + EF BP 570-620 / LP 750 filters, respectively. Exposure time was 40 ms and EM gain was 300 for both channels. SMLs were calculated in 2D by Zeiss ZEN Black 2.1 SP3 software using x,y 2D Gauss fit with point spread function (PSF) half width 177.9 nm, peak mask size 9 pixels and peak intensity to noise 6 and accounted for overlap in 2D with max cluster size 10. SMLs were rendered in ZEN software with 10 nm/px resolution and 1x PSF expansion factor. The data were model-based drift corrected in ZEN. Two channels were aligned using tetraspec beads fiducial markers for affine calibration. Drift-corrected and aligned localization coordinates were exported as text files. Text files were converted into csv files and imported using self-written macro (Hoboth et al., 2021a) into the ImageJ2 (Rueden et al., 2017) plug-in ThunderSTORM, visualized by normalized Gaussian method (Ovesny et al., 2014).

opencc-by-4.0Apr 2024View details →

zenodo40/100

Ditrect STORM imaging and image reconstruction of the transcription initiation marker P-S5 and nuclear PI(3,4)P2 indirectly immunolabeled with AF647 (red) and AF555 (green) in control cells.

U-2 OS cells were grown in DMEM with 10% FBS at 37°C and 5% CO2. Cells were plated one day before staining in ~50% confluence on the high-precision 12 mm round coverslips treated with Hellmanex, sonicated, washed, dried and sterilized. Cells were control treated with 1:000 DMSO in the culture media. U2OS cells were washed twice with PBS (pH 7.4) and fixed for 30 min in 2% PFA in PBS, washed 3-times for 5 min with PBS, then permeabilized in 0.1% Triton X-100 in PBS for 20 min, washed 3-times for 5 min by PBS and blocked in filtered 5% BSA in PBS for 30 min. Cells were incubated for 45 min with rabbit polyclonal IgG anti-RNAPII CTD P-S5 (Abcam ab5131) 3 µg/mL and mouse monoclonal IgG2 anti-PI(3,4)P2 (Z-P034; Echelon Biosci. Inc., USA) 5 µg/mL  in 5% BSA in PBS, washed 3-times for 5 min in PBS and incubated for 30 min with donkey anti-mouse IgG AF555 (Invitrogen A31570) 10 µg/mL and goat anti-rabbit IgG AF647 (Invitrogen A21245) 10 µg/mL diluted in 5% BSA in PBS. Then the cells were washed 3-times for 5 min in PBS, post-fixed for 15 min in 2% PFA in PBS and washed 3-times for 5 min in PBS. All procedures were performed at RT and the cells were stored in PBS in the fridge overnight prior imaging. Coverslips with cells were mounted in the Chamlide chamber (Live Cell Instrument, Korea) and covered with imaging buffer (PBS pH 7.4, 50 mM MEA). Single-molecule localizations (SMLs) data were acquired by Zeiss Elyra PS.1 equipped with HR Diode 642-150 and HR DPSS 561-200 lasers, Alpha Plan-Apochromat 100x/1.46 oil DIC M27 Elyra objective and Andor EM CCD iXon DU 897 camera and Zeiss ZEN Black 2.1 SP3 software (Zeiss). AF647 and AF555 photo-switching was achieved by HiLo illumination and TIRF HP FOV with 100% power of 642nm or 561nm laser, and the signal was acquired via MBS 642 + EF LP 655 and MBS 561 + EF BP 570-620 / LP 750 filters, respectively. Exposure time was 40 ms and EM gain was 300 for both channels. SMLs were calculated in 2D by Zeiss ZEN Black 2.1 SP3 software using x,y 2D Gauss fit with point spread function (PSF) half width 177.9 nm, peak mask size 9 pixels and peak intensity to noise 6 and accounted for overlap in 2D with max cluster size 10. SMLs were rendered in ZEN software with 10 nm/px resolution and 1x PSF expansion factor. The data were model-based drift corrected in ZEN. Two channels were aligned using tetraspec beads fiducial markers for affine calibration. Drift-corrected and aligned localization coordinates were exported as text files. Text files were converted into csv files and imported using self-written macro (Hoboth et al., 2021a) into the ImageJ2 (Rueden et al., 2017) plug-in ThunderSTORM, visualized by normalized Gaussian method (Ovesny et al., 2014).

opencc-by-4.0Apr 2024View details →

zenodo40/100

Appendix 2 in A new subfamily classification of the Citrus family (Rutaceae) based on six nuclear and plastid markers

Appendix 2. Details about the character states of all included taxa for the 13 morphological and karyological characters.<table><thead><tr><th></th><th></th><th></th><th></th><th></th><th></th><th colspan="2">Characters</th><th></th><th></th><th></th><th></th><th></th><th></th></tr></thead><tbody><tr><th>Taxon</th><td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td colspan="2">6 7</td><td>8</td><td>9</td><td>10</td><td>11</td><td>12</td><td>13</td></tr><tr><th>Acmadenia</th><td>0</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Acradenia</th><td>1</td><td>1</td><td>2, 3</td><td>1</td><td>1</td><td>1</td><td>2</td><td>1</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Acronychia</th><td>0, 1</td><td>1</td><td>1</td><td>1</td><td>0, 1</td><td>1</td><td>1</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Adenandra</th><td>0</td><td>0, 1</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>0</td><td>2, 3</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Adiscanthus</th><td>0</td><td>0</td><td>2</td><td>0</td><td>0</td><td>1</td><td>2</td><td>1</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Aegle</th><td>0, 1</td><td>0</td><td>1, 2</td><td>2</td><td>0</td><td>4</td><td>0</td><td>0</td><td>0, 1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Aeglopsis</th><td>0, 1</td><td>0</td><td>1, 2, 3</td><td>1, 2</td><td>0</td><td>4</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Afraegle</th><td>1</td><td>0</td><td>1, 2, 3</td><td>2</td><td>0</td><td>4</td><td>0</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Agathosma A</th><td>0</td><td>0, 1, 2</td><td>2, 3</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Agathosma B</th><td>0</td><td>0, 1, 2</td><td>2, 3</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Agathosma C</th><td>0</td><td>0, 1, 2</td><td>2, 3</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Amyris</th><td>0, 1</td><td>0, 1</td><td>1, 2, 3</td><td>1</td><td>3</td><td>1</td><td>1</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Andreadoxa</th><td>0</td><td>0</td><td>2</td><td>0</td><td>1</td><td>1</td><td>2</td><td>1</td><td>?</td><td>0</td><td>1</td><td>0</td><td>0</td></tr><tr><th>Angostura</th><td>0, 1</td><td>0</td><td>2</td><td>0</td><td>1</td><td>1</td><td>2</td><td>1</td><td>?</td><td>0</td><td>0, 1</td><td>1</td><td>0</td></tr><tr><th>Asterolasia</th><td>0</td><td>0</td><td>2</td><td>1</td><td>0, 1</td><td>0, 1</td><td>2</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Atalantia</th><td>0</td><td>0</td><td>1, 2, 3</td><td>1</td><td>0</td><td>0, 1</td><td>5</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Balfourodendron</th><td>1</td><td>1</td><td>1</td><td>0</td><td>0</td><td>1</td><td>4</td><td>1</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Balsamocitrus</th><td>0, 1</td><td>0</td><td>2</td><td>1</td><td>0</td><td>4</td><td>0</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Bergera</th><td>0, 1</td><td>0</td><td>1, 2</td><td>1</td><td>0</td><td>?</td><td>0</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Boenninghausenia</th><td>1</td><td>0</td><td>1</td><td>1</td><td>1</td><td>2, 3</td><td>2</td><td>2</td><td>0</td><td>2</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Boronia A</th><td>0, 1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>0, 1, 2</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Boronia B</th><td>0, 1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>0, 1, 2</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Boronia C</th><td>0, 1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>0, 1, 2</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Boronia D (Boronella)</th><td>0</td><td>1, 2</td><td>1</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Bosistoa</th><td>0, 1</td><td>0</td><td>2</td><td>1</td><td>1</td><td>2, 3</td><td>2</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Bottegoa</th><td>1</td><td>0</td><td>1, 2</td><td>0</td><td>0</td><td>0</td><td>4</td><td>1</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Bouchardatia</th><td>0, 1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>3</td><td>2</td><td>0, 1</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Brombya</th><td>0</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>2</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Burkillanthus</th><td>0, 1</td><td>0</td><td>2</td><td>1</td><td>0</td><td>4</td><td>5</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Calodendrum</th><td>0</td><td>1, 2</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>0</td><td>2</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Casimiroa</th><td>0, 1</td><td>0</td><td>1, 2, 3</td><td>0</td><td>0</td><td>1, 2, 3</td><td>0, 1</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Cedrelopsis</th><td>1</td><td>0</td><td>2</td><td>0</td><td>0</td><td>1, 2</td><td>2</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>1</td></tr><tr><th>Chloroxylon</th><td>1</td><td>0</td><td>2</td><td>1</td><td>0</td><td>3</td><td>2</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>1</td></tr><tr><th>Choisya</th><td>1</td><td>1</td><td>1, 2</td><td>1</td><td>1</td><td>1</td><td>2</td><td>2</td><td>2</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Chorilaena</th><td>0</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Citropsis</th><td>0, 1</td><td>0</td><td>1, 2</td><td>1</td><td>0</td><td>0</td><td>5</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Citrus A</th><td>0</td><td>0</td><td>1, 2, 3</td><td>2</td><td>0</td><td>1, 2, 3</td><td>5</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Citrus B</th><td>0</td><td>0</td><td>1, 2, 3</td><td>2</td><td>0</td><td>1, 2, 3</td><td>5</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Taxon</th><td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td colspan="2">Characters 6 7</td><td>8</td><td>9</td><td>10</td><td>11</td><td>12</td><td>13</td></tr><tr><th>Citrus C</th><td>0</td><td>0</td><td>1, 2, 3</td><td>2</td><td>0</td><td>1, 2, 3</td><td>5</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Clausena</th><td>1</td><td>0</td><td>1, 2</td><td>1</td><td>0</td><td>1</td><td>0</td><td>0</td><td>0, 1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Clymenia (Citrus s.l.)</th><td>0</td><td>0</td><td>1, 2, 3</td><td>2</td><td>0</td><td>1, 2, 3</td><td>5</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Cneoridium</th><td>0</td><td>1</td><td>1</td><td>1</td><td>3</td><td>1, 2</td><td>0</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Cneorum</th><td>0</td><td>0</td><td>0, 1</td><td>0</td><td>0</td><td>1</td><td>1</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Coatesia</th><td>0</td><td>0</td><td>2</td><td>0</td><td>1</td><td>1</td><td>2</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Coleonema</th><td>0</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>0</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Comptonella</th><td>0, 1</td><td>1</td><td>1</td><td>0, 1</td><td>0, 1</td><td>1</td><td>1</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Conchocarpus A</th><td>0, 1</td><td>0, 1</td><td>1, 2</td><td>0</td><td>1</td><td>1</td><td>2</td><td>1</td><td>?</td><td>0</td><td>0, 1</td><td>1</td><td>0</td></tr><tr><th>Conchocarpus B (Almeidea)</th><td>0</td><td>0</td><td>2</td><td>0</td><td>1</td><td>1</td><td>2</td><td>1</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Correa</th><td>0</td><td>1</td><td>1</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>1</td><td>0</td><td>0</td><td>1</td><td>0</td></tr><tr><th>Crossosperma</th><td>1</td><td>1</td><td>1</td><td>1</td><td>0</td><td>0, 1</td><td>1</td><td>1</td><td>?</td><td>0</td><td>0</td><td>0</td><td>1</td></tr><tr><th>Crowea</th><td>0</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Cyanothamnus</th><td>0, 1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Desmotes</th><td>0</td><td>1</td><td>2</td><td>0</td><td>1</td><td>1</td><td>2</td><td>1</td><td>?</td><td>0</td><td>1</td><td>1</td><td>0</td></tr><tr><th>Dictamnus</th><td>1</td><td>0</td><td>2</td><td>1</td><td>0</td><td>2</td><td>2</td><td>2</td><td>1</td><td>2</td><td>1</td><td>0</td><td>0</td></tr><tr><th>Dictyoloma</th><td>1</td><td>0</td><td>2</td><td>0</td><td>1</td><td>2</td><td>2</td><td>1</td><td>?</td><td>0</td><td>0</td><td>0</td><td>1</td></tr><tr><th>Dinosperma</th><td>0, 1</td><td>0, 1, 2</td><td>1</td><td>1</td><td>1</td><td>1</td><td>2</td><td>0, 1</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Diosma</th><td>0</td><td>0, 1</td><td>2</td><td>0, 1</td><td>1</td><td>0, 1</td><td>2</td><td>0</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Diplolaena</th><td>0</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Drummondita</th><td>0</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Dryades</th><td>0</td><td>0</td><td>2</td><td>0</td><td>1</td><td>1</td><td>2</td><td>?</td><td>?</td><td>0</td><td>0, 1</td><td>1</td><td>0</td></tr><tr><th>Dutaillyea</th><td>0, 1</td><td>1</td><td>1</td><td>1</td><td>0</td><td>0, 1</td><td>1</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Empleurum</th><td>0</td><td>0</td><td>1</td><td>0</td><td>1, 3</td><td>0, 1</td><td>2</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Eremocitrus (Citrus s.l.)</th><td>0</td><td>0</td><td>1, 2, 3</td><td>2</td><td>0</td><td>1, 2, 3</td><td>5</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Eriostemon</th><td>0</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Ertela</th><td>1</td><td>0, 1</td><td>2</td><td>0</td><td>1</td><td>1</td><td>2</td><td>1</td><td>?</td><td>1, 2</td><td>1</td><td>1</td><td>0</td></tr><tr><th>Erythrochiton</th><td>0</td><td>0</td><td>2</td><td>0</td><td>1</td><td>1</td><td>2</td><td>1</td><td>?</td><td>0</td><td>0, 1</td><td>1</td><td>0</td></tr><tr><th>Esenbeckia</th><td>0, 1</td><td>0, 1</td><td>2, 3</td><td>0</td><td>0, 1</td><td>1</td><td>2</td><td>0, 1</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Euchaetis</th><td>0</td><td>0, 1</td><td>2</td><td>0, 1</td><td>1</td><td>0, 1</td><td>2</td><td>0</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Euodia</th><td>0, 1</td><td>1</td><td>1</td><td>0, 1</td><td>1</td><td>1</td><td>2</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Fagaropsis</th><td>1</td><td>1</td><td>1, 2, 3</td><td>0, 1</td><td>0</td><td>0</td><td>1</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Feroniella</th><td>1</td><td>0</td><td>1, 2, 3</td><td>2</td><td>0</td><td>1, 2, 3</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Flindersia</th><td>0, 1</td><td>0, 1</td><td>2</td><td>1</td><td>0</td><td>1, 2, 3</td><td>2</td><td>0</td><td>1, 3</td><td>0</td><td>0</td><td>0</td><td>1</td></tr><tr><th>Fortunella (Citrus s.l.)</th><td>0</td><td>0</td><td>1, 2, 3</td><td>2</td><td>0</td><td>1, 2, 3</td><td>5</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Galipea</th><td>0, 1</td><td>0</td><td>2</td><td>0</td><td>0</td><td>1</td><td>2</td><td>1</td><td>?</td><td>0</td><td>1</td><td>1</td><td>0</td></tr><tr><th>Geijera</th><td>0</td><td>0</td><td>0, 1, 2</td><td>0</td><td>1</td><td>1</td><td>3</td><td>1</td><td>3</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Geleznowia</th><td>0</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Glycosmis</th><td>0, 1</td><td>0</td><td>1, 2</td><td>0, 1</td><td>0</td><td>0, 1</td><td>0</td><td>0</td><td>0, 2</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Halfordia</th><td>0</td><td>0</td><td>2</td><td>1</td><td>0</td><td>0</td><td>1</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Haplophyllum</th><td>0, 1</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1, 2, 3</td><td>2</td><td>2</td><td>0</td><td>1, 2</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Harrisonia</th><td>1</td><td>0</td><td>1, 2</td><td>1</td><td>0</td><td>0</td><td>1</td><td>1</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Helietta</th><td>1</td><td>0, 1</td><td>1, 2</td><td>0</td><td>0</td><td>1</td><td>4</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Hortia</th><td>0</td><td>0</td><td>2</td><td>0</td><td>0</td><td>1</td><td>0, 1</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Taxon</th><td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td colspan="2">Characters 6 7</td><td>8</td><td>9</td><td>10</td><td>11</td><td>12</td><td>13</td></tr><tr><th>Ivodea</th><td>0</td><td>0, 1, 2</td><td>1, 2</td><td>0, 1</td><td>1</td><td>1</td><td>2</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Leionema A</th><td>0</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0, 1</td><td>0</td></tr><tr><th>Leionema B</th><td>0</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0, 1</td><td>0</td></tr><tr><th>Limonia</th><td>1</td><td>0</td><td>1, 2, 3</td><td>1</td><td>0</td><td>4</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Lunasia</th><td>0</td><td>0</td><td>0</td><td>0</td><td>1</td><td>0</td><td>2</td><td>0, 1</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Luvunga</th><td>0, 1</td><td>0</td><td>0, 1, 2</td><td>1</td><td>0</td><td>0, 1</td><td>0</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Maclurodendron</th><td>0</td><td>1</td><td>1</td><td>1</td><td>0</td><td>1</td><td>1</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Macrostylis</th><td>0</td><td>0, 1</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Medicosma</th><td>0, 1</td><td>0, 1, 2</td><td>1</td><td>0, 1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Melicope A</th><td>0, 1</td><td>1</td><td>1</td><td>0, 1</td><td>1</td><td>1</td><td>3</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Melicope B</th><td>0, 1</td><td>1</td><td>1</td><td>0</td><td>0, 1</td><td>1</td><td>3</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Melicope C</th><td>0, 1</td><td>1, 2</td><td>1</td><td>0, 1</td><td>0, 1</td><td>1</td><td>3</td><td>2</td><td>0, 1, 2</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Melicope D</th><td>0, 1</td><td>1</td><td>1</td><td>0</td><td>1</td><td>0, 1</td><td>3</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Melicope E (Platydesma)</th><td>0</td><td>0, 1</td><td>1</td><td>1</td><td>0</td><td>2, 3</td><td>3</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Merope</th><td>0</td><td>0</td><td>2</td><td>1</td><td>0</td><td>1, 2</td><td>0</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Merrillia</th><td>1</td><td>0</td><td>2</td><td>1</td><td>0</td><td>3</td><td>0</td><td>0</td><td>?</td><td>0</td><td>1</td><td>0</td><td>0</td></tr><tr><th>Metrodorea</th><td>0, 1</td><td>1</td><td>2</td><td>0</td><td>0</td><td>1</td><td>2</td><td>0, 1</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Microcitrus (Citrus s.l.)</th><td>0</td><td>0</td><td>1, 2, 3</td><td>2</td><td>0</td><td>1, 2, 3</td><td>5</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Microcybe</th><td>0</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Micromelum</th><td>0, 1</td><td>0</td><td>2</td><td>1</td><td>0</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Monanthocitrus</th><td>0</td><td>0</td><td>2</td><td>1</td><td>0</td><td>1, 2, 3</td><td>0</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Muiriantha</th><td>0</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>1</td><td>0</td><td>0</td><td>1</td><td>0</td></tr><tr><th>Murraya</th><td>1</td><td>0</td><td>1, 2</td><td>1</td><td>0</td><td>0, 1</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Myrtopsis</th><td>0</td><td>1</td><td>1, 2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>?</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Naringi</th><td>0, 1</td><td>0</td><td>1, 2</td><td>1</td><td>0</td><td>0, 1</td><td>5</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Nematolepis</th><td>0</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0, 1</td><td>0</td></tr><tr><th>Neobyrnesia</th><td>0</td><td>1</td><td>1</td><td>0</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Neoraputia</th><td>0, 1</td><td>0, 1</td><td>2</td><td>0</td><td>0</td><td>1</td><td>2</td><td>1</td><td>?</td><td>0</td><td>1</td><td>1</td><td>0</td></tr><tr><th>Neoschmidea</th><td>0</td><td>0</td><td>1, 2</td><td>1</td><td>1</td><td>1</td><td>2</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Orixa</th><td>0</td><td>0</td><td>1</td><td>0</td><td>1</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Pamburus</th><td>0</td><td>0</td><td>1, 2</td><td>1</td><td>0</td><td>1</td><td>0</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Paramignya</th><td>0</td><td>0</td><td>1, 2</td><td>1</td><td>0</td><td>0, 1</td><td>0</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Peltostigma</th><td>0, 1</td><td>0</td><td>0, 1, 2, 3</td><td>1, 2</td><td>0</td><td>0, 1</td><td>2</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Pentaceras</th><td>1</td><td>0</td><td>2</td><td>1</td><td>1</td><td>1</td><td>1, 4</td><td>1</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Perryodendron</th><td>0</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>3</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Phebalium</th><td>0</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>1, 2</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Phellodendron</th><td>1</td><td>1</td><td>2</td><td>0</td><td>0</td><td>0</td><td>1</td><td>1</td><td>2</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Philotheca A</th><td>0</td><td>0</td><td>1, 2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>1, 2</td><td>0</td><td>0</td><td>0, 1</td><td>0</td></tr><tr><th>Philotheca B</th><td>0</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>1, 2</td><td>0</td><td>0</td><td>0, 1</td><td>0</td></tr><tr><th>Phyllosma</th><td>0</td><td>0</td><td>2</td><td>0, 1</td><td>1</td><td>0, 1</td><td>2</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Picrella</th><td>0, 1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>0, 1</td><td>1</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Pilocarpus</th><td>0, 1</td><td>0</td><td>1, 2</td><td>0</td><td>0</td><td>0, 1</td><td>2</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Pitavia</th><td>0</td><td>1, 2</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Pitaviaster</th><td>0</td><td>1</td><td>1</td><td>0</td><td>1</td><td>1</td><td>1</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Taxon</th><td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td colspan="2">Characters 6 7</td><td>8</td><td>9</td><td>10</td><td>11</td><td>12</td><td>13</td></tr><tr><th>Pleiospermium</th><td>0, 1</td><td>0</td><td>1, 2</td><td>1</td><td>0</td><td>1</td><td>5</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Plethadenia</th><td>1</td><td>0, 1</td><td>1</td><td>1</td><td>2</td><td>0</td><td>2</td><td>?</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Poncirus (Citrus s.l.)</th><td>1</td><td>0</td><td>1, 2, 3</td><td>2</td><td>0</td><td>1, 2, 3</td><td>5</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Psilopeganum</th><td>1</td><td>0</td><td>1, 2</td><td>1</td><td>1</td><td>2, 3</td><td>2</td><td>2</td><td>?</td><td>2</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Ptaeroxylon</th><td>1</td><td>1</td><td>1</td><td>0</td><td>0</td><td>0</td><td>2</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>1</td></tr><tr><th>Ptelea</th><td>1</td><td>0</td><td>1, 2, 3</td><td>0</td><td>0</td><td>1</td><td>2, 4</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Rauia</th><td>0, 1</td><td>0</td><td>2</td><td>0</td><td>0</td><td>1</td><td>2</td><td>1</td><td>?</td><td>0</td><td>0</td><td>1</td><td>0</td></tr><tr><th>Ravenia</th><td>0, 1</td><td>1</td><td>2</td><td>0</td><td>1</td><td>1</td><td>2</td><td>1</td><td>?</td><td>0</td><td>1</td><td>1</td><td>0</td></tr><tr><th>Rhadinothamnus</th><td>0</td><td>0</td><td>2</td><td>1</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0, 1</td><td>0</td></tr><tr><th>Ruta</th><td>1</td><td>0</td><td>1, 2</td><td>1</td><td>0</td><td>4</td><td>2</td><td>2</td><td>0, 1, 2</td><td>1, 2</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Sarcomelicope</th><td>0</td><td>1, 2</td><td>1</td><td>1</td><td>0, 1</td><td>1</td><td>1</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Severinia</th><td>0</td><td>0</td><td>1, 2, 3</td><td>1</td><td>0</td><td>0, 1</td><td>5</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Sheilanthera</th><td>0</td><td>0</td><td>2</td><td>0</td><td>1</td><td>0, 1</td><td>2</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Sigmatanthus</th><td>1</td><td>0</td><td>2</td><td>0</td><td>1</td><td>1</td><td>2</td><td>1</td><td>?</td><td>0</td><td>1</td><td>1</td><td>0</td></tr><tr><th>Skimmia</th><td>0</td><td>0</td><td>1, 2, 3</td><td>0</td><td>0</td><td>0</td><td>1</td><td>2</td><td>1, 2</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Sohnreyia</th><td>1</td><td>0</td><td>2</td><td>0</td><td>0</td><td>0, 1</td><td>4</td><td>0, 1, 2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Spathelia</th><td>1</td><td>0</td><td>2</td><td>0</td><td>0</td><td>0, 1</td><td>4</td><td>0, 1, 2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Spiranthera</th><td>1</td><td>0</td><td>2</td><td>0</td><td>0</td><td>1</td><td>2</td><td>1</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Swinglea</th><td>1</td><td>0</td><td>2</td><td>1</td><td>0</td><td>4</td><td>0</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Tetractomia</th><td>0</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>3</td><td>2</td><td>?</td><td>0</td><td>0</td><td>0</td><td>1</td></tr><tr><th>Tetradium</th><td>1</td><td>1</td><td>1, 2</td><td>0</td><td>1</td><td>0, 1</td><td>3</td><td>2</td><td>1, 2</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Thamnosma</th><td>0</td><td>0</td><td>1</td><td>1</td><td>0</td><td>2, 3</td><td>2</td><td>2</td><td>0</td><td>0, 1</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Toxosiphon</th><td>0, 1</td><td>0</td><td>2</td><td>0</td><td>0</td><td>1</td><td>2</td><td>1</td><td>?</td><td>0</td><td>1</td><td>1</td><td>0</td></tr><tr><th>Triphasia</th><td>0, 1</td><td>0</td><td>0, 2</td><td>1</td><td>0</td><td>0, 1</td><td>0</td><td>0</td><td>0, 1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Vepris</th><td>0, 1</td><td>0, 1</td><td>1</td><td>0, 1</td><td>0, 3</td><td>0, 1</td><td>1</td><td>2</td><td>1</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Wenzelia</th><td>0</td><td>0</td><td>2</td><td>1</td><td>0</td><td>3</td><td>0</td><td>0</td><td>?</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Zanthoxylum A</th><td>0, 1</td><td>0</td><td>1, 2</td><td>0</td><td>1</td><td>1</td><td>3</td><td>1, 2</td><td>1, 2, 3</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Zanthoxylum B</th><td>0, 1</td><td>0</td><td>1, 2</td><td>0</td><td>1</td><td>1</td><td>3</td><td>1, 2</td><td>1, 2, 3</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Zanthoxylum C</th><td>0, 1</td><td>0</td><td>0, 1, 2, 3</td><td>0</td><td>1</td><td>1</td><td>3</td><td>1, 2</td><td>1, 2, 3</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Zanthoxylum D (Toddalia)</th><td>0, 1</td><td>0</td><td>1, 2, 3</td><td>0</td><td>0</td><td>1</td><td>1</td><td>2</td><td>1, 2</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><th>Zieria</th><td>0, 1</td><td>1</td><td>1</td><td>0</td><td>1</td><td>0, 1</td><td>2</td><td>2</td><td>1, 2</td><td>0</td><td>0</td><td>0</td><td>0</td></tr></tbody></table>1. Leaf type: (0) simple/unifoliolate; (1) compound. 2. Phyllotaxis: (0) alternate; (1) opposite; (2) whorled. 3. Flower merosity: (0) 3-merous; (1) 4-merous; (2) 5-merous; (3) polymerous. 4. Stamen whorls: (0) haplostemonous; (1) diplostemonous; (2) more than two whorls. 5. Carpel connation: (0) syncarpous; (1) syncarpous (style only); (2) apocarpous (no joined style); (3) one carpel. 6. Ovules per locule: (0) 1; (1) 2; (2) 3–5; (3) 6–10; (4)>10. 7. Fruit type: (0) berry; (1) drupe; (2) dehiscent with seeds detached; (3) dehiscent with seeds attached; (4) samara; (5) hesperidium. 8. Endosperm: (0) lacking; (1) scanty; (2) copious. 9. Chromosomes: (0) 9–10; (1) 14–18; (2) 28–36; (3) ≥64. 10. Growth form: (0) shrubs or trees; (1) subshrubs; (2) perennial herbs. 11. Flower symmetry: (0) actinomorphic; (1) zygomorphic. 12. Tubular corolla: (0) petals free and not forming a tube; (1) petals coherent or connate and forming a corolla tube. 13. Seeds: (0) not winged; (1) winged(Continues)(Continues)

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CX-MS Datasets for "Comprehensive Structure and Functional Adaptations of the Yeast Nuclear Pore Complex"

This repository contains chemical cross-linking mass spectrometry data of affinity-purified Yeast nuclear pore complexes. Data Files Description: NPC_XL_Identification_Inter_Crosslinked.csv: Inter-protein cross-links identified by pLink 2. NPC_XL_spectra.mgf: MS2 spectra data for the identified cross-links. NPC_XL_proteins.fasta : Protein sequences used for search. Sample Processing: NPCs were immuno-purified from Mlp1 tagged S. cerevisiae strains (Kim et al., 2018). After native elution, 1.0 mM disuccinimidyl suberate (DSS) was added and the sample was incubated at 25ºC for 40 min with shaking (1,200 rpm). The reaction was quenched by adding a final concentration of 50 mM freshly prepared ammonium bicarbonate and incubating for 20 min with shaking (1,200 rpm) at 25ºC. The sample (50 µg) was then concentrated and denatured at 98ºC for 5 min in a solubilization buffer (10% solution of 1-dodecyl-3-methylimidazolium chloride (C12-mim-Cl) in 50 mM ammonium bicarbonate, pH 8.0, 100 mM DTT). After denaturation, the sample was centrifuged at 21,130 g for 10 min and the supernatant was transferred to a 100 kDa MWCO ultrafiltration unit (MRCF0R100, Microcon). The sample was quickly spun at 1,000 g for 2 min and washed twice with 50 mM ammonium bicarbonate. After alkylation (50 mM iodoacetamide), the cross-linked NPC in-filter was digested by trypsin and lysC O/N at 37ºC. After proteolysis, the sample was recovered by centrifugation and peptides were fractionated into 10-12 fractions by using a stage tip self-packed with basic C18 resins (Dr. Masch GmbH). Fractionated samples were pooled prior to LC/MS analysis. Desalted cross-link peptides were dissolved in the sample loading buffer (5% Methanol, 0.2% FA), separated with an automated nanoLC device (nLC1200, Thermo Fisher), and analyzed by an Orbitrap Q Exactive HFX (Pharma mode) mass spectrometer (Thermo Fisher) as previously described (Xiang et al., 2020; Xiang et al., 2021). Briefly, peptides were loaded onto an analytical column (C18, 1.6 μm particle size, 100 Å pore size, 75 μm × 25 cm; IonOpticks) and eluted using a 120-min liquid chromatography gradient. The flow rate was approximately 300 nl/min. The spray voltage was 1.7 kV. The QE HF-X instrument was operated in the data-dependent mode, where the top 10 most abundant ions (mass range 380 – 2,000, charge state 4 - 8) were fragmented by high-energy collisional dissociation (HCD). The target resolution was 120,000 for MS and 15,000 for tandem MS (MS/MS) analyses. The quadrupole isolation window was 1.8 Th; the maximum injection time for MS/MS was set at 200 ms. Data Processing: The raw data were searched with pLink2 (Chen et al., 2019b). An initial MS1 search window of 5 Da was allowed to cover all isotopic peaks of the cross-linked peptides. The data were automatically filtered using a mass accuracy of MS1 ≤ 10 ppm (parts per million) and MS2 ≤ 20 ppm of the theoretical monoisotopic (A0) and other isotopic masses (A+1, A+2, A+3, and A+4) as specified in the software. Other search parameters included cysteine carbamidomethyl as a fixed modification and methionine oxidation as a variable modification. A maximum of two trypsin missed-cleavage sites was allowed. The initial search results were obtained using a default 5% false discovery rate (FDR) expected by the target-decoy search strategy. Spectra were manually verified to improve data quality (Kim et al., 2018; Shi et al., 2014). Cross-linking data were analyzed and plotted with CX-Circos (http://cx-circos.net).

opencc-by-4.0Nov 2021View details →

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Automated metabolic assignment: Semi-supervised learning in metabolic analysis employing two dimensional Nuclear Magnetic Resonance (NMR)

This dataset is related to the paper “Automated metabolic assignment: Semi-supervised learning in metabolic analysis employing two dimensional Nuclear Magnetic Resonance (NMR)”. https://www.sciencedirect.com/science/article/pii/S2001037021003792?via%3Dihub The dataset comprises horizontal and vertical frequencies of 2D NMR TOCSY of breast cancer-tissue sample. 2D TOCSY was acquired by employing a broadband high resolution 600.13 MHz (B0 = 14.1 T) NMR Bruker spectrometer (AVANCE III 600 with the Bruker magnet ASCEND 600) supported with the room temperature probe (BBO model-Bruker) and Magic Angle Spinning (MAS) probehead. 1D and 2D NMR spectra acquisition and processing were achieved by using the TopSpin software package 3.6. There are two files: BreastCancerMetabolites.csv: First column: numerical labels of the metabolites. Each number represent a metabolite. In total, there are 27 metabolites with multiple multiplets per metabolite. Second and third column: Horizontal and vertical frequencies for each metabolite. Labels.csv: The corresponding metabolites names.

opencc-by-4.0Aug 2021View details →

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Fig. 17. Phylogenetic hypothesis using nuclear gene sequences TMO-4C4 and 18S in A New Genus and Species of Pygmy Pipehorse from Taitokerau Northland, Aotearoa New Zealand, with a Redescription of Acentronura Kaup, 1853 and Idiotropiscis Whitley, 1947 (Teleostei, Syngnathidae)

Fig. 17. Phylogenetic hypothesis using nuclear gene sequences TMO-4C4 and 18S retrieved with Maximum Likelihood (ML), Maximum Parsimony (MP), and Bayesian Inference (MrBayes), representing 17 species from clade 6 from the analysis of Hamilton et al. (2017) and the new taxon. Tree rooted with the southern Australian trunk-brooder pipefish Heraldia nocturna. Nodal support at the generic level is shown in ML/MP/MrBayes order. See Data Accessibility for tree file.

opencc-by-4.0Sep 2021View details →

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Multiple Nuclei HeLa cell ground truth images with four labels (nuclear envelope, nucleus, rest of the cell, and background) for deep learning architecture training.

This is a data set that contains labelled HeLa cell images, indicating the four different classes - nuclear envelope, nucleus, rest of the cell, and background. Similar ground truth have been published for this data set, but in this case, multiple nuclei have been labelled, whilst previous ones only focused on the central cell (https://doi.org/10.5281/zenodo.3874949) Details of the imaging, preparation and segmentation have been published in: <ul> <li>Cefa Karabağ, Martin L. Jones, Christopher J. Peddie, Anne E. Weston, Lucy M. Collinson, Constantino Carlos Reyes-Aldasoro. Segmentation and Modelling of the Nuclear Envelope of HeLa Cells Imaged with Serial Block Face Scanning Electron Microscopy. J. Imaging 2019, 5(9), 75; <a href="https://doi.org/10.3390/jimaging5090075">https://doi.org/10.3390/jimaging5090075</a></li> <li>Cefa Karabağ, Martin L. Jones, Christopher J. Peddie, Anne E. Weston, Lucy M. Collinson, Constantino Carlos Reyes-Aldasoro. Semantic segmentation of HeLa cells: An objective comparison between one traditional algorithm and four deep-learning architectures, PLOS ONE, 2020;  <a href="https://doi.org/10.1371/journal.pone.0230605">https://doi.org/10.1371/journal.pone.0230605</a></li> <li> Cefa Karabağ, Martin L. Jones, Constantino Carlos Reyes-Aldasoro, Segmentation of the Plasma Membrane of HeLa Cells, J. Imaging 2021, 7(6), 93; <a href="https://doi.org/10.3390/jimaging7060093">https://doi.org/10.3390/jimaging7060093</a> </li> </ul> <ul> <li>The data sets are freely available through EMPIAR: http://dx.doi.org/10.6019/EMPIAR-10094 EMPIAR.</li> </ul>

opencc-by-4.0Mar 2022View details →

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Supplementary information: The nuclear-spin-forbidden rovibrational transitions of water from first principles

Supplementary material to the manuscript "The nuclear-spin-forbidden rovibrational transitions of water from first principles" by Andrey Yachmenev, Guang Yang, Emil Zak, Sergei Yurchenko, and Jochen Küpper, J. Chem. Phys., submitted. <a href="https://arxiv.org/abs/2203.07945"> arXiv:2203.07945</a> The data set contains hyperfine (spin-rovibrational) energies and dipole transition spectrum of water molecule (H216O), calculated using variational approach <a href="https://github.com/Trovemaster/TROVE">TROVE</a> and <a href="https://github.com/CFEL-CMI/richmol">RichMol</a>, and included spin-rotational and spin-spin hyperfine interactions. In addition, the data set includes HDF5-type richmol database file h2o_p48_j40_rovib.h5 (see <a href="https://github.com/CFEL-CMI/richmol">https://github.com/CFEL-CMI/richmol</a>) containing rovibrational energies, matrix elements of nuclear spin-rotation, nuclear spin-spin, electric dipole, and electric quadrupole tensor operators of H216O, calculated using variational approach TROVE. <ul> <li>h2o_exomol_F.states and h2o_exomol_F.trans - hyperfine linelist of water stored in the ExoMol format (see, e.g., <a href="https://doi.org/10.1016/j.jms.2016.05.002">J. Molec. Spectrosc., 327, 73-94 (2016)</a>). The two files contain a set of hyperfine states with assignments and a set of dipole transitions (Einstein A-coefficients), respectively. The states in h2o_exomol_F.states file are arranged by quantum number of total angular momentum F = I + J (spin + rotation) in ascending order.</li> <li>h2o_exomol_J.states and h2o_exomol_J.trans - contain same data as h2o_exomol_F.states and h2o_exomol_F.trans files, except that the states in h2o_exomol_J.states file are arranged by rotational quantum number (J) in ascending order.</li> <li>h2o_p48_j40_rovib.h5 - Richmol HDF5 database file for H216O containing rovibrational energies (in cm-1), matrix elements of nuclear spin-rotation (in kHz), spin-spin (in kHz), molecular electric dipole moment (in Debye), and molecular electric quadrupole moment (in a.u.) operators. For details on how to read this file, see <a href="https://github.com/CFEL-CMI/richmol">Richmol GitHub repository</a> and <a href="https://richmol.readthedocs.io/en/latest/">Richmol documentation</a> (or contact Andrey Yachmenev at andrey.yachmenev@cfel.de).</li> <li>ortho_para_transitions.txt  - table with strongest predicted ortho-para transitions in H216O at T = 296 K with the 10−36 cm/molecule intensity cut-off. An example of hyperfine energies and hyperfine dipole spectrum calculation for water using h2o_p48_j40_rovib.h5 file from this repository may be found in the <a href="https://github.com/CFEL-CMI/richmol">Richmol GitHub repository's</a> examples folder: <a href="https://github.com/CFEL-CMI/richmol/tree/develop/examples/hyperfine">https://github.com/CFEL-CMI/richmol/tree/develop/examples/hyperfine</a></li> </ul> Structure of h2o_exomol_F.states and h2o_exomol_J.states files: <table align="left"> <thead> <tr> <th scope="col">Column No.</th> <th scope="col">Kind  </th> <th scope="col">Meaning                    </th> </tr> </thead> <tbody> <tr> <td>1</td> <td>int</td> <td>state ID number</td> </tr> <tr> <td>2</td> <td>float</td> <td>hyperfine state energy relative to the ZPE, in cm-1</td> </tr> <tr> <td>3</td> <td>int</td> <td>state degeneracy</td> </tr> <tr> <td>4</td> <td>int</td> <td>value of F quantum number (total spin-rotational angular momentum)</td> </tr> <tr> <td>5</td> <td>str</td> <td>state symmetry in C2v</td> </tr> <tr> <td>6</td> <td>int</td> <td>value of J quantum number (total rotational angular momentum)</td> </tr> <tr> <td>7</td> <td>str</td> <td>symmetry of state's rotational component in C2v</td> </tr> <tr> <td>8</td> <td>int</td> <td>value of ka quantum number (a-axis projection of rotational angular momentum)</td> </tr> <tr> <td>9</td> <td>int</td> <td>value of kc quantum number (c-axis projection of rotational angular momentum)</td> </tr> <tr> <td>10</td> <td>int</td> <td>value of v1 vibrational quantum number</td> </tr> <tr> <td>11</td> <td>int</td> <td>value of v2 vibrational quantum number</td> </tr> <tr> <td>12</td> <td>int</td> <td>value of v3 vibrational quantum number</td> </tr> <tr> <td>13</td> <td>int</td> <td>value of I quantum number (total nuclear spin)</td> </tr> <tr> <td>14</td> <td>float</td> <td>reference rovibrational state energy (i.e., without hyperfine effects) relative to the ZPE, in cm-1</td> </tr> </tbody> </table> Structure of h2o_exomol_F.trans and h2o_exomol_J.trans files: <table> <thead> <tr> <th scope="col">Column No.</th> <th scope="col">Kind</th> <th scope="col">Meaning</th> </tr> </thead> <tbody> <tr> <td>1</td> <td>int</td> <td>ID number of final transition state (col. no. 1 in h2o_exomol_F.states or h2o_exomol_J.states file)</td> </tr> <tr> <td>2</td> <td>int</td> <td>ID number of initial transition state (col. no. 1 in h2o_exomol_F.states or h2o_exomol_J.states file)</td> </tr> <tr> <td>3</td> <td>float</td> <td>Einstein A-coefficient, in s-1</td> </tr> <tr> <td>4</td> <td>float</td> <td>Transition wavenumber, in cm-1</td> </tr> </tbody> </table>

opencc-by-4.0Feb 2022View details →

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NMMA: A nuclear-physics and multi-messenger astrophysics framework to analyze binary neutron star mergers

Data release associated with the preprint "NMMA: A nuclear-physics and multi-messenger astrophysics framework to analyze binary neutron star mergers" Data includes: EOS files: <ul> <li>5000 eos files with radius (km), mass (Msun), and tidal deformability as columns stored under eos/eos_data</li> <li>prior probabilities for the EOSs are stored in eos/eos_prior_probability.dat</li> </ul> Posterior samples: <ul> <li>Posterior samples based on the analysis of GW170817 and AT2017gfo stored in posterior_samples/GW170817-AT2017gfo_posterior_samples.dat</li> <li>Posterior samples based on the analysis of GW170817,  AT2017gfo, and the afterglow of GRB170817A are stored in posterior_samples/GW170817-AT2017gfo-GRB170817A_afterglow_posterior_samples.dat</li> </ul>

opencc-by-4.0May 2022View details →

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Standardized nuclear markers improve and homogenize species delimitation in Metazoa

Species are the fundamental units of life and evolution. Their recognition is essential for science and society. Molecular methods have been increasingly employed for the identification of animal species, despite several challenges. Here, we explore with genomic data from nine animal lineages a set of nuclear markers, namely metazoan-level universal single-copy orthologs (metazoan USCOs), for their use in species delimitation. Our data sets include arthropods and vertebrates. We use various data assembly strategies and employ coalescent-based species inference as well as population admixture analyses and phenetic methods. We demonstrate that metazoan USCOs well distinguish closely related morphospecies and consistently outperform classical mitochondrial DNA barcoding in discriminating closely related species in different animal taxa. USCOs overcome the general shortcomings of mitochondrial DNA barcodes, and due to standardization across Metazoa, also those of other approaches. They accurately assign samples not only to lower but also to higher taxonomic levels. Metazoan USCOs provide a powerful and unifying framework for DNA-based species delimitation and taxonomy in animals and their employment could result in a more efficient use of research data and resources.

opencc-zeroNov 2022View details →

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Characterization of the nuclear proteome of Chlamydomonas in response to salt stress

Supplementary Files and Figures for the manuscript "Characterization of the nuclear proteome of Chlamydomonas in response to salt stressCharacterization of the nuclear proteome of Chlamydomonas in response to salt stress"

opencc-by-4.0May 2022View details →

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A higher-level nuclear phylogenomic study of the carrot family (Apiaceae)

Premise The carrot family (Apiaceae) comprises 466 genera, which include many well-known crops (e.g., aniseed, caraway, carrots, celery, coriander, cumin, dill, fennel, parsley, and parsnips). Higher-level phylogenetic relationships among subfamilies, tribes, and other major clades of Apiaceae are not fully resolved. This study aims to address this important knowledge gap. Methods Target sequence capture with the universal Angiosperms353 probe set was used to examine phylogenetic relationships in 234 genera of Apiaceae, representing all four currently recognized subfamilies (Apioideae, Azorelloideae, Mackinlayoideae, and Saniculoideae). Recovered nuclear genes were analyzed using both multispecies coalescent and concatenation approaches. Results We recovered hundreds of nuclear genes even from old and poor-quality herbarium specimens. Of particular note, we placed with strong support three incertae sedis genera (Platysace, Klotzchia, and Hermas); all three occupy isolated positions, with Platysaceresolved as sister to all remaining Apiaceae. We placed nine genera (Apodicarpum, Bonannia, Grafia, Haplosciadium, Microsciadium, Physotrichia, Ptychotis, Tricholaser, Xatardia) that have never previously been included in any molecular phylogenetic study. Conclusions We provide support for the maintenance of the four existing subfamilies of Apiaceae, while recognizing that Hermas, Klotzschia, and the Platysace clade may each need to be accommodated in additional subfamilies (pending improved sampling). The placement of the currently apioid genus Phlyctidocarpa can be accommodated by the expansion of subfamily Saniculoideae, although adequate morphological synapomorphies for this grouping are yet to be defined. This is the first phylogenetic study of the Apiaceae using high-throughput sequencing methods and represents an unprecedented evolutionary framework for the group.

opencc-by-4.0Jun 2022View details →

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Design Principles for the Development of Gd(III) Polarizing Agents for Magic Angle Spinning Dynamic Nuclear Polarization

This is the raw dataset for publication  Design Principles for the Development of Gd(III) Polarizing Agents for Magic Angle Spinning Dynamic Nuclear Polarization. with the DOI of 10.1021/acs.jpcc.2c01721. It contains all NMR, EPR raw data and the MATLAB codes that are used in this paper. For details, please refer to the readme file.

opencc-by-4.0Jun 2022View details →

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1H Hyperpolarization of Solutions by Overhauser Dynamic Nuclear Polarization with 13C-1H Polarization Transfer

The dataset here contains the raw data used for the publication 10.1021/acs.jpclett.2c01956 There are two folders and one readme file. NMR data in JCAMP or Topspin are in these two folders and are organised according to the figure or table mentioned in the publication. For details, please refer to the readme file.

opencc-by-4.0Aug 2022View details →

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Molecular Dynamics of Jelly Candies by Means of Nuclear Magnetic Resonance Relaxometry

1H spin-lattice Nuclear Magnetic Resonance relaxation studies have been performed for different kinds of Haribo jelly and Vidal jelly in a very broad frequency range from about 10 kHz to 10 MHz to obtain insight into the dynamic and structural properties of jelly candies on the molecular level. This extensive data set has been thoroughly analyzed revealing three dynamic processes, referred to as slow, intermediate and fast dynamics occurring on the timescale of 10−6 s, 10−7 s and 10−8 s, respectively. The parameters have been compared for different kinds of jelly for the purpose of revealing their characteristic dynamic and structural properties as well as to enquire into how increasing temperature affects these properties. It has been shown that dynamic processes in different kinds of Haribo jelly are similar (this can be treated as a sign of their quality and authenticity) and that the fraction of confined water molecules is reduced with increasing temperature. Two groups of Vidal jelly have been identified. For the first one, the parameters (dipolar relaxation constants and correlation times) match those for Haribo jelly. For the second group including cherry jelly, considerable differences in the parameters characterizing their dynamic properties have been revealed.

opencc-by-4.0Aug 2022View details →

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Global patterns of nuclear and mitochondrial genetic diversity in marine fishes

Genetic diversity is a fundamental component of biodiversity. Examination of global patterns of genetic diversity can help highlight mechanisms underlying species diversity, though a recurring challenge has been that patterns may vary by molecular marker. Here, we compiled 6862 observations of genetic diversity from 492 species of marine fish and tested among hypotheses for diversity gradients: the founder effect hypothesis, the kinetic energy hypothesis, and the productivity-diversity hypothesis. We fit generalized linear mixed effect models (GLMMs) and explored the extent to which various macroecological drivers (latitude, longitude, temperature (SST), and chlorophyll-a concentration) explained variation in genetic diversity. We found that mitochondrial genetic diversity followed geographic gradients similar to those of species diversity, being highest near the Equator, particularly in the Coral Triangle, while nuclear genetic diversity did not follow clear geographic patterns. Despite these differences, all genetic diversity metrics were correlated with chlorophyll-a concentration, while mitochondrial diversity was also positively associated with SST. Our results provide support for the kinetic energy hypothesis, which predicts that elevated mutation rates at higher temperatures increase mitochondrial but not necessarily nuclear diversity, and the productivity-diversity hypothesis, which posits that resource-rich regions support larger populations with greater genetic diversity. Overall, these findings reveal how environmental variables can influence mutation rates and genetic drift in the ocean, caution against using mitochondrial macro-genetic patterns as proxies for whole-genome diversity, and aid in defining global gradients of genetic diversity.

opencc-zeroApr 2024View details →

ScienceDex guides

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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.

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Allen Brain Atlas

Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.

allen-brain-atlas

neuroscienceopenDocumentation, web resources, and API references are available online.

Last verified 2026-04-30Open record

Annotated Behaviour and Observability Dataset (ABODe)

ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.

abode-home-cage

behavioral-neuroscienceopenThe DataShare record exposes download links for annotations, documentation, license text, and the zipped per-snippet data directory.

Last verified 2026-04-30Open record

DANDI Archive for NWB datasets

DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.

dandi-nwb

electrophysiologyopenPublished Dandiset metadata and archive endpoints are available through the production DANDI API.

Last verified 2026-04-30Open record

International Brain Laboratory public data

The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.

ibl

behavioral-neuroscienceopenPublic sessions can be searched and loaded from the IBL public data server through ONE.

Last verified 2026-04-29Open record

OpenNeuro

OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.

openneuro

neuroscienceopenPublished datasets are available on demand over the internet.

Last verified 2026-04-29Open record