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1,363 results for “phenotypic data”
Supporting data and code for: Myzus persicae resistance to neonicotinoids - unravelling the contribution of different mechanisms to phenotype
<p>This is the first release of the final data and code for the article accepted for publication in <em>Pest Management Science</em> journal. It contains the necessary scripts to produce most of the analyses and figures of the manuscript. All the necessary data can be found in the 'data' folder.</p>
Data and scripts used in the paper entitled "Led Color Gradient As A New Screening Tool For Rapid Phenotyping Of Plant Responses To Light Quality" by Pierre LEJEUNE et al.
<p>Images, script and data used in the paper entitled "Led Color Gradient As A New Screening Tool For Rapid Phenotyping Of Plant Responses To Light Quality" by Pierre LEJEUNE, Anthony FRATAMICO, Frédéric BOUCHÉ, Samuel HUERGA-FERNÁNDEZ, Pierre TOCQUIN, Claire PÉRILLEUX</p>
Phenotype, genotype and fitness data related to genetic analysis of praziquantel response in schistosome parasites.
<p>These data are related to the study of the Genetic analysis of praziquantel response in schistosome parasites implicates a Transient Receptor Potential channel.</p> <p>Mass treatment with praziquantel (PZQ) monotherapy is the mainstay for schistosomiasis treatment. This drug shows imperfect cure rates in the field and parasites showing reduced PZQ response can be selected in the laboratory, but the extent of resistance in <em>Schistosoma mansoni</em> populations is unknown. We examined the genetic basis of variation in PZQ response in a <em>S. mansoni</em> population (SmLE-PZQ-R) selected with PZQ in the laboratory: 35% of these worms survive high dose (73 µg/mL) PZQ treatment. We used genome wide association to map loci underlying PZQ response. The major chr. 3 peak contains a transient receptor potential (Sm.TRPM_PZQ) channel (Smp_246790), activated by nanomolar concentrations of PZQ. PZQ response shows recessive inheritance and marker-assisted selection of parasites at a single Sm.TRPM_PZQ SNP enriched populations of PZQ-resistant (PZQ-ER) and sensitive (PZQ-ES) parasites showing >377 fold difference in PZQ response. The PZQ-ER parasites survived treatment in rodents better than PZQ-ES. Resistant parasites show 2.25-fold lower expression of Sm.TRPM_PZQ than sensitive parasites. Specific chemical blockers of Sm.TRPM_PZQ enhanced PZQ resistance, while Sm.TRPM_PZQ activators increased sensitivity. A single SNP in Sm.TRPM_PZQ differentiated PZQ-ER and PZQ-ES lines, but mutagenesis showed this was not involved in PZQ response, suggesting linked regulatory changes. We surveyed Sm.TRPM_PZQ sequence variation in 259 parasites from the New and Old World revealing one nonsense mutation that results in a truncated protein with no PZQ-binding site. Our results demonstrate that Sm.TRPM_PZQ underlies variation in PZQ response in <em>S. mansoni</em> and provides an approach for monitoring emerging PZQ-resistance alleles in schistosome elimination programs.</p> <p>This dataset is divided in 3 folders. Each folder has a readme detailing its content.</p> <p><strong>1-Phenotyping_data</strong></p> <p>This folder includes the data tables related to the phenotyping of the worms performed during this study. The phenotype measured was the viability of worms following PZQ treatment (i.e., PZQ response). This viability was assessed microscopically or using worm lactate production released in culture media.</p> <p>The data correspond to the following experiments:</p> <ul> <li>PZQ response of single adult male worms from SmLE and SmLE-PZQ-R populations to different doses of PZQ. This data was used to determine the PZQ IC50 of each population.</li> <li>Lactate production from single SmLE-PZQ-R adult male worms and correlation with visual observation. This was a proof-of-principle that lactate production can be used to efficiently and unbiasedly phenotype schistosome adult male worms in response to PZQ drug.</li> <li>PZQ response of single SmLE-PZQ-R adult male worms. These worms were then divided in low and high producer in response to PZQ and used to perform a genome-wide association study.</li> <li>PZQ response of single adult male worms from SmLE-PZQ-ER and SmLE-PZQ-ES populations to different doses of PZQ. This data was used to determine the PZQ IC50 of each population.</li> <li>PZQ response of single adult male worms from SmLE-PZQ-ER and SmLE-PZQ-ES populations in presence of Sm.TRPM_PZQ blocker (MB2) and activator (MV1) with and without PZQ drug.</li> <li>In vivo PZQ response of schistosome worms from SmLE-PZQ-ER and SmLE-PZQ-ES populations.</li> </ul> <p><strong>2-Genotyping_data</strong></p> <p>This folder includes the data tables related to the genotyping of the worms performed during this study. Worms were genotyping using PCR-RFLP (genotyping of single nucleotide polymorphisms (SNPs) on chr2 and chr3 QTLs) or using qPCR (genotyping of a copy number variation (CNV) on chr3 QTL).</p> <p>The data correspond to the following experiment:</p> <ul> <li>Association between PZQ response of single adult male worms from SmLE-PZQ-R population and their respective genotype on chromosome 2 (SNP) and chromosome 3 (SNP and CNV) loci.</li> </ul> <p><strong>3-Fitness_data</strong></p> <p>This folder includes the data tables related to the fitness of the parasite populations. We collected data regarding:</p> <ul> <li>The number of surviving and infected snails after exposure to SmLE-PZQ-ER or SmLE-PZQ-ES miracidia.</li> <li>The number of adult worms recovered from golden Syrian female hamsters exposed to SmLE-PZQ-ER or SmLE-PZQ-ES cercariae.</li> </ul> <p>All the data were collected during 12 generations of parasites and are used to evaluate a potential impact of PZQ resistance on the parasite fitness.</p>
Data files for manuscript "Prenatal phenotype of PNKP-related primary microcephaly associated with variants in the FHA and Phosphatase domain"
<p>#2021-08-26<br> #Summary<br> This ZIP-file contains the Excel files used for the clinical and variant analyses of the PNKP protein/gene for the manuscript "Prenatal phenotype of PNKP-related primary microcephaly associated with variants in the FHA and Phosphatase domain".</p> <p>#Folder structure<br> ./ (parent directory containing this README file and all subfolders)<br> ./Clinical/ (contains an Excel sheet with complete clinical data of 4 affected individuals)<br> ./Variants/ (contains an Excel sheet with all variant annotation and domain information used in 6 sheets)</p> <p>#Files and checksums<br> 4B5BE914E77EB6511DF3DB94C6918E45 ./Clinical/FileS2_PNKP_Clinical.xlsx<br> 0228C6EF543D597C1C800B8D78E3097D ./Variants/FileS3_PNKP_Variants.xlsx</p>
Data from: Floral scents of a deceptive plant are hyperdiverse and under population-specific phenotypic selection
<p>Floral scent is a key mediator in plant–pollinator interactions; however, little is known to what extent intraspecific scent variation is shaped by phenotypic selection, with no information yet in deceptive plants. We recorded 289 scent compounds in deceptive moth fly-pollinated <i>Arum maculatum </i>from various populations north vs. south of the Alps, the highest number so far reported in a single plant species. Scent and fruit set differed between regions, and some, but not all differences in scent could be explained by differential phenotypic selection in northern vs. southern populations. Our study is the first to provide evidence that phenotypic selection is involved in shaping geographic patterns of floral scent in deceptive plants. The hyperdiverse scent of <i>A. maculatum</i> might result from the plant's imitation of various brood substrates of its pollinators.</p>
Agronomic, rheological and nutritional phenotypic data of 50 spelt varieties grown at 3 locations in Switzerland during 2 growing seasons (2021-2022)
<p>This dataset contains agronomic, rheological, and nutritional parameters of 50 winter spelt varieties tested during 2 growing seasons (2021-2022) at 3 locations in Switzerland. The dataset has been used to investigate the links between genotype and phenotype of spelt varieties, published in https://doi.org/10.1007/s10681-024-03400-8.</p> <p>The field trials were performed under the Swiss Extenso (low input) conditions, conducted by Agroscope and DSP, and under organic conditions, performed by GZPK. </p> <h3>Methods </h3> <p><em>Field trials </em></p> <div>Field trials were set up over the course of two growing seasons – 2020/2021, 2021/2022 – in three sites across the Swiss Central Plateau. The experimental sites were located in Changins (46°19′ N 6°14′ E, 455m a.s.l), Delley (46°55′ N 6°58′ E, 494m a.s.l) and Feldbach (47°14'24.00" N, 8°47'9.60" E, 410m a.s.l.).</div> <div>Each variety was grown in a plot of 7.1 m<sup>2</sup> (1.5 m*4.7 m) in Changins and Delley, and 4.5 m<sup>2</sup> (1.5 m*3 m) in Feldbach. We replicated the experiment three times per location. At each site, we used a complete randomized block design, with plots being randomized within each block. Density of sowing was 180 spikelets/m<sup>2</sup>. Plots were sowed mechanically each autumn. In Changins and Delley, the plots were mechanically fertilized with 100 kg N/ha (ammonium nitrate), applied in two splits (60 at heading stage—40 at flowering stage). In Feldbach, the fields were treated organically, and therefore no synthetic fertilizer was applied.</div> <div> </div> <div> </div> <div><em>Agronomic and morphological characteristics </em></div> <div> <p>For each plot, we recorded the heading date as the day of the year, in which 50% of the ears of the plot had fully emerged from the flag leaf. Once the plants and ears were fully developed, plant height was measured in each plot, by taking the average height in centimeters from the ground to the top of five random ears, excluding awns.</p> <p>At maturity, we harvested each plot with a combine harvester (Zürn 150, Schontal-Westernhausen, Switzerland). The harvested grains were weighed first, dehusked, sorted and cleaned with a sieve cleaner, and then weighted again. We measured specific weight and water content using a Dickey–John machine (GAC 2100). Grain yield was subsequently standardized to 15% of humidity. Protein content (%) was measured at the plot level with a near-infrared instrument (ProxiMate™, Büchi instruments). Thousand kernel weight (TKW, g), as well as kernel length and width (mm), were measured at the plot level with a Marvin seed analyzer (GTA Sensorik, Neubrandenburg, Germany).</p> <p>Additional measurements in Changins: we computed harvest index for each plot by cutting 30 individual culms just before harvest. Plants were cut just above the ground, oven-dried for 3 days at 80 °C and then weighed. We then threshed, dehusked, sieved and weighed the obtained grains. The harvest index was computed by taking the ratio of grain mass over total mass.</p> <p> </p> <p><em>Rheological characteristics </em></p> </div> <div> <p>At all sites, Zeleny sedimentation value (mL) was assessed based on the International Association for Cereal Science and Technology standard method 116/1.The analyses were performed by the analytical laboratory of DSP, Delley, at the variety level for each site—i.e., grains from the three replicates per site were pooled together and subsequently milled.</p> <p>Additional measurements in Changins were done for each variety, based on a pooled sample of the three replicates. Extensograph properties of the obtained dough were assessed according to ICC standard method 114/1; area under curve (energy, cm2), resistance to extension at 5 cm extension (EE), and extensibility of the dough (mm) were measured. The analyses were performed by the accredited laboratory “Versuchsanstalt für Getreideverarbeitung” based in Austria (<a href="https://www.vfg.or.at/">https://www.vfg.or.at/</a>).</p> <p> </p> <p><em>Nutritional characteristics </em></p> </div> <div> </div> <div>We assessed the structure of starch (amylose content) and the fatty acid composition for each variety in Changins. These analyses were done by pooling grains from the three replicates in Changins and milling them. The amylose and amylopectin contents of starch were determined enzymatically via an assay based on the precipitation of amylopectin complexes with the lectin concanavalin A, according to K-Amy 06/18. The fatty acid composition was analyzed by GC-FAME, via in situ transesterification, according to the method of Ampuero Kragten et al. (<a title="Kragten SA, Collomb M, Dubois S, Stoll P (2014) Determination of fatty acid composition in feed: analytical methods. Agrarforschung Schweiz 5(9):330&ndash;337" href="https://link.springer.com/article/10.1007/s10681-024-03400-8#ref-CR36">2014</a>). These analyses were performed at the accredited analytical laboratory of Agroscope, Posieux.</div> <div> </div> <div>Kragten SA, Collomb M, Dubois S, Stoll P (2014) Determination of fatty acid composition in feed: analytical methods. Agrarforschung Schweiz 5(9):330–337</div> <div> </div> <div> </div> <div><em>DNA extraction & Genotyping </em></div> <div> </div> <div>DNA was extracted from all cultivars, and sent to TraitGenetics (SGS institute Frenius, Gatersleben DE) for SNP genotyping on the 25 K XT Infinium array for wheat.</div> <div> </div> <div> </div>
Proteomics and metabolomics data associated with the end-of-life phenotype Smurf
<p>Data obtained from whole bodies of mated females of genotype Drs-GFP at 20 and 40 days for proteomics and 30 days for metabolomics.</p> <p>The data are cited in the following preprint :</p> <p>Smurfness-based two-phase model of ageing helps deconvolve the ageing transcriptional signature</p> <p>Flaminia Zane, Hayet Bouzid, Sofia Sosa Marmol, <a href="http://orcid.org/0000-0003-2448-4022"> View ORCID Profile</a>Savandara Besse, Julia Lisa Molina, <a href="http://orcid.org/0000-0002-9579-5250"> View ORCID Profile</a>Céline Cansell, Fanny Aprahamian, <a href="http://orcid.org/0000-0001-6356-1006"> View ORCID Profile</a>Sylvère Durand, Jessica Ayache, <a href="http://orcid.org/0000-0001-7709-2116"> View ORCID Profile</a>Christophe Antoniewski, <a href="http://orcid.org/0000-0002-6574-6511"> View ORCID Profile</a>Michael Rera</p> <p>doi: https://doi.org/10.1101/2022.11.22.517330</p>
Data for: From individual behaviors to collective outcomes: fruiting body formation in Dictyostelium as a group-level phenotype
<p>Collective phenotypes, which arise from the interactions among individuals, can be important for the evolution of higher levels of biological organization. However, how a group's composition determines its collective phenotype remains poorly understood. When starved, cells of the social amoeba <em>Dictyostelium discoideum</em> cooperate to build a multicellular fruiting body, and the morphology of the fruiting body is likely advantageous to the surviving spores. We assessed how the number of strains, as well as their genetic and geographic relationships to one another, impact the group's morphology and productivity. We find that some strains consistently enhance or detract from the productivity of their groups, regardless of the identity of the other group members. We also detect extensive pairwise and higher-order genotype interactions, which collectively have a large influence on the group phenotype. Whereas previous work in <em>Dictyostelium</em> has focused almost exclusively on whether spore production is equitable when strains cooperate to form multicellular fruiting bodies, our results suggest a previously unrecognized impact of chimeric co-development on the group phenotype. Our results demonstrate how interactions among members of a group influence collective phenotypes and how group phenotypes might in turn impact selection on the individual.</p>
Functional and molecular characterization of suicidality factors using phenotypic and genome-wide data
<p>GWAS summary statistics for Functional and molecular characterization of suicidality factors using phenotypic and genome-wide data published in Molecular Psychiatry by Quintero Reis A, Newton BA, Kessler R, Polimanti R, and Wendt FR.</p>
Data from: The CellPhe toolkit for cell phenotyping using time-lapse imaging and pattern recognition
<p>With phenotypic heterogeneity in whole cell populations widely recognised, the demand for quantitative and temporal analysis approaches to characterise single cell morphology and dynamics has increased. We present CellPhe, a pattern recognition toolkit for the unbiased characterisation of cellular phenotypes within time-lapse videos. CellPhe imports tracking information from multiple segmentation and tracking algorithms to provide automated cell phenotyping from different imaging modalities, including fluorescence. To maximise data quality for downstream analysis, our toolkit includes automated recognition and removal of erroneous cell boundaries induced by inaccurate tracking and segmentation. We provide an extensive list of features extracted from individual cell time series, with custom feature selection to identify variables that provide the greatest discrimination for the analysis in question. Using ensemble classification for accurate prediction of cellular phenotype and clustering algorithms for the characterisation of heterogeneous subsets, we validate and prove adaptability using different cell types and experimental conditions.</p>
Data for: Decoupling of sexual signals and their underlying morphology facilitates rapid phenotypic diversification
<p>How novel phenotypes evolve is challenging to imagine because traits are often underlain by numerous integrated phenotypic components, and changes to any one form can disrupt the function of the entire module. Yet novel phenotypes do emerge, and research on adaptive phenotypic evolution suggests that complex traits can diverge while either maintaining existing form-function relationships or through innovations that alter form-function relationships. How these alternate routes contribute to sexual signal evolution is poorly understood, despite the role of sexual signals in generating biodiversity. In Hawaiian populations of the Pacific field cricket, male song attracts both female crickets and a deadly acoustically orienting parasitoid fly. In response to this conflict between natural and sexual selection, male crickets have evolved altered wing morphologies multiple times, resulting in loss and dramatic alteration of sexual signals. More recently, we and others have observed a radical increase in sexual signal variation and the underlying morphological structures that produce song. We conducted the first combined analysis of form (wing morphology), function (emergent signal), and receiver responses to characterize novel variation, test alternative hypotheses about form-function relationships (Form-Function Continuity vs. Form-Function Decoupling) and investigate underlying mechanistic changes and fitness consequences of novel signals. We identified three sound-producing male morphs (one previously undescribed, named "rattling") and found that relationships between morphology and signals have been rewired (Form-Function Decoupling), rapidly and repeatedly, through the gain, loss, and alteration of morphological structures, facilitating the production of signals that exist in novel phenotypic space. By integrating across a hierarchy of phenotypes, we uncovered divergent morphs with unique solutions to the challenge of attracting mates while evading fatal parasitism.</p>
Data from: How important are functional and developmental constraints on phenotypic evolution? An empirical test with the stomatal anatomy of flowering plants
<p>Quantifying the relative contribution of functional and developmental constraints on phenotypic variation is a longstanding goal of macroevolution, but it is often difficult to distinguish different types of constraints. Alternatively, selection can limit phenotypic (co)variation if some trait combinations are generally maladaptive. The anatomy of leaves with stomata on both surfaces (amphistomatous) presents a unique opportunity to test the importance of functional and developmental constraints on phenotypyic evolution. The key insight is that stomata on each leaf surface encounter the same functional and developmental constraints, but potentially different selective pressures because of leaf asymmetry in light capture, gas exchange, and other features. Independent evolution of stomatal traits on each surface implies that functional and developmental constraints alone likely do not explain trait covariance. Packing limits on how many stomata can fit into a finite epidermis and cell-size-mediated developmental integration are hypothesized to constrain variation in stomatal anatomy. The simple geometry of the planar leaf surface and knowledge of stomatal development makes it possible to derive equations for phenotypic (co)variance caused by these constraints and compare them with data. We analyzed evolutionary covariance between stomatal density and length in amphistomatous leaves from 236 phylogenetically independent contrasts using a robust Bayesian model. Stomatal anatomy on each surface diverges partially independently, meaning that packing limits and developmental integration are not sufficient to explain phenotypic (co)variation. Hence, (co)variation in ecologically important traits like stomata arises in part because there is a limited range of evolutionary optima. We show how it is possible to evaluate the contribution of different constraints by deriving expected patterns of (co)variance and testing them using similar but separate tissues, organs, or sexes.</p>
Data from: Population divergence in heat and drought responses of a coastal plant: from metabolic phenotypes to plant morphology and growth
<p>This dataset supports the article "Population divergence in heat and drought responses of a coastal plant: from metabolic phenotypes to plant morphology and growth", which is under minor revision in Journal of Experimental Botany. The study addresses the combined effects of and plant population origin, drought and heat stress on plant growth, plant morphology and the leaf metabolome. The data were assessed in Northern and Southern European individuals of <em>Cakile maritma</em> (See Rocket). An R-script containing all statistical analyses that have been implemented with these data is also provided.</p>
Data and Code for Publication "Inferring human neutral genetic variation from craniodental phenotypes"
<p>Data and code for publication: H. Rathmann et al., Inferring human neutral genetic variation from craniodental phenotypes. PNAS Nexus.</p> <p>The repository contains:</p> <ul> <li>“<em>R code for DP-DG analysis.txt</em>”: R code for testing levels of neutral evolutionary signals preserved in five craniodental data types: cranial metrics, dental metrics, cranial non-metric traits, dental non-metric traits, and craniodental metrics and non-metric traits combined.</li> </ul> <ul> <li>“<em>Cranial metric data.csv</em>”: Dataset consisting of 37 cranial metric variables for 26 worldwide modern populations, provided in a comma-separated values file format. The data were collected by T. Hanihara and originally presented in the publication titled: T. Hanihara, Comparison of craniofacial features of major human groups. <em>Am. J. Phys. Anthropol.</em> 99, 389–412 (1996) (<a href="https://doi.org/10.1002/(SICI)1096-8644(199603)99:3%3c389::AID-AJPA3%3e3.0.CO;2-S">https://doi.org/10.1002/(SICI)1096-8644(199603)99:3<389::AID-AJPA3>3.0.CO;2-S</a>).</li> </ul> <ul> <li>“<em>Dental metric data.csv</em>”: Dataset comprising 28 dental metric variables for 26 worldwide modern populations, provided in a comma-separated values file format. The data were collected by T. Hanihara and originally presented in the publication titled: T. Hanihara, H. Ishida, Metric dental variation of major human populations. <em>Am. J. Phys. Anthropol.</em> 128, 287–298 (2005) (<a href="https://doi.org/10.1002/ajpa.20080">https://doi.org/10.1002/ajpa.20080</a>).</li> </ul> <ul> <li>“<em>Cranial non-metric trait data.csv</em>”: Dataset consisting of 24 cranial non-metric trait variables for 26 worldwide modern populations, provided in a comma-separated values file format. The data were collected for the most part by T. Hanihara and presented in the publication titled: T. Hanihara, H. Ishida, Y. Dodo, Characterization of biological diversity through analysis of discrete cranial traits. <em>Am. J. Phys. Anthropol.</em> 121, 241–251 (2003) (<a href="https://doi.org/10.1002/ajpa.10233">https://doi.org/10.1002/ajpa.10233</a>).</li> </ul> <ul> <li>“<em>Dental non-metric trait data.csv</em>”: Dataset comprising 25 dental non-metric trait variables for 26 worldwide modern populations, provided in a comma-separated values file format. The data were collected by C. G. Turner II, G. R. Scott, and J. D. Irish. This individual-level dataset was artificially created from population-level trait frequency information presented in the publications: G. R. Scott, J. D. Irish, <em>Human Tooth Crown and Root Morphology </em>(Cambridge University Press, 2017) (<a href="https://doi.org/10.1017/9781316156629">https://doi.org/10.1017/9781316156629</a>); and: J. D. Irish, A. Morez, L. Girdland Flink, E. L. W. Phillips, G. R. Scott, Do dental nonmetric traits actually work as proxies for neutral genomic data? Some answers from continental- and global-level analyses. <em>Am. J. Phys. Anthropol. </em>172, 347–375 (2020) (<a href="https://doi.org/10.1002/ajpa.24052">https://doi.org/10.1002/ajpa.24052</a>).</li> </ul> <ul> <li>“<em>SNP data.txt</em>”: Dataset comprising 8,821 SNP markers for 26 worldwide modern populations, provided in a genepop file format. The data were obtained from various published sources: I. Lazaridis et al., Ancient human genomes suggest three ancestral populations for present-day Europeans. <em>Nature </em>513, 409–413 (2014) (<a href="https://doi.org/10.1038/nature13673">https://doi.org/10.1038/nature13673</a>); P. Qin, M. Stoneking, Denisovan ancestry in east Eurasian and native American populations. <em>Mol. Biol. Evol. </em>32, 2665–2674 (2015) (<a href="https://doi.org/10.1093/molbev/msv141">https://doi.org/10.1093/molbev/msv141</a>); P. Skoglund et al., Genomic insights into the peopling of the Southwest Pacific. <em>Nature </em>538, 510–513 (2016) (<a href="https://doi.org/10.1038/nature19844">https://doi.org/10.1038/nature19844</a>); M. R. Nelson et al., The Population Reference Sample, POPRES: a resource for population, disease, and pharmacological genetics research. <em>Am. J. Hum. Genet. </em>83, 347–358 (2008) (<a href="https://doi.org/10.1016/j.ajhg.2008.08.005">https://doi.org/10.1016/j.ajhg.2008.08.005</a>); J. K. Pickrell, J. K. Pritchard, Inference of population splits and mixtures from genome-wide allele frequency data. <em>PLoS Genet. </em>8, e1002967 (2012) (<a href="https://doi.org/10.1371/journal.pgen.1002967">https://doi.org/10.1371/journal.pgen.1002967</a>); A. Bergström et al., Insights into human genetic variation and population history from 929 diverse genomes. <em>Science </em>367 (2020) (<a href="https://doi.org/10.1126/science.aay5012">https://doi.org/10.1126/science.aay5012</a>); B. M. Henn et al., Genomic ancestry of North Africans supports back-to-Africa migrations. <em>PLoS Genet. </em>8, e1002397 (2012) (<a href="https://doi.org/10.1371/journal.pgen.1002397">https://doi.org/10.1371/journal.pgen.1002397</a>); S. Mallick et al., The Simons Genome Diversity Project: 300 genomes from 142 diverse populations. <em>Nature </em>538, 201–206 (2016) (<a href="https://doi.org/10.1038/nature18964">https://doi.org/10.1038/nature18964</a>); Lao et al., Correlation between genetic and geographic structure in Europe. <em>Curr. Biol. </em>18, 1241–1248 (2008) (<a href="https://doi.org/10.1016/j.cub.2008.07.049">https://doi.org/10.1016/j.cub.2008.07.049</a>); and M. Lipson et al., Population Turnover in Remote Oceania Shortly after Initial Settlement. <em>Curr. Biol. </em>28, 1157-1165.e7 (2018) (<a href="https://doi.org/10.1016/j.cub.2018.02.051">https://doi.org/10.1016/j.cub.2018.02.051</a>).</li> </ul> <p>For population and variable names and abbreviations, see Supplementary Information in: H. Rathmann et al., Inferring human neutral genetic variation from craniodental phenotypes. PNAS Nexus.</p>
Data from: Accelerated high-throughput imaging and phenotyping system for small organisms
<p>Studying the complex web of interactions in biological communities requires large multifactorial experiments with sufficient statistical power. Automation tools reduce the time and labor associated with setup, data collection, and analysis in experiments that untangle these webs. We developed tools for high-throughput experimentation (HTE) in duckweeds, small aquatic plants that are amenable to autonomous experimental preparation and image-based phenotyping. We showcase the abilities of our HTE system in a study with 6,000 experimental units grown across 2,000 treatments. These automated tools facilitated the collection and analysis of time-resolved growth data, which revealed finer dynamics of plant-microbe interactions across environmental gradients. Altogether, our HTE system can run experiments with up to 11,520 experimental units and can be adapted for other small organisms.</p>
Data from: Maximum mutational robustness in genotype-phenotype maps follows a self-similar blancmange-like curve
<div class="section abstract"> <p>Phenotype robustness, defined as the average mutational robustness of all the genotypes that map to a given phenotype, plays a key role in facilitating neutral exploration of novel phenotypic variation by an evolving population. By applying results from coding theory, we prove that the maximum phenotype robustness occurs when genotypes are organised as bricklayer's graphs, so called because they resemble the way in which a bricklayer would fill in a Hamming graph. The value of the maximal robustness is given by a fractal continuous everywhere but differentiable nowhere sums-of-digits function from number theory. Interestingly, genotype-phenotype (GP) maps for RNA secondary structure and the HP model for protein folding can exhibit phenotype robustness that exactly attains this upper bound. By exploiting properties of the sums-of-digits function, we prove a lower bound on the deviation of the maximum robustness of phenotypes with multiple neutral components from the bricklayer's graph bound, and show that RNA secondary structure phenotypes obey this bound. Finally, we show how robustness changes when phenotypes are coarse-grained and derive a formula and associated bounds for the transition probabilities between such phenotypes.</p> </div>
Genotype, phenotype and linkage data for Mimulus parishii x M. cardinalis hybrid incompatibility study
<p>The evolution of genomic incompatibilities causing postzygotic barriers to hybridization is a key step in species divergence. Incompatibilities take two general forms – structural divergence between chromosomes leading to severe hybrid sterility in F<sub>1</sub> hybrids and epistatic interactions between genes causing reduced fitness of hybrid gametes or zygotes (Dobzhansky-Muller incompatibilities). Despite substantial recent progress in understanding the molecular mechanisms and evolutionary origins of both types of incompatibility, how each behaves across multiple generations of hybridization remains relatively unexplored. Here, we use genetic mapping in F<sub>2</sub> and RIL hybrid populations between the phenotypically divergent but naturally hybridizing monkeyflowers <em>Mimulus cardinalis</em> and <em>M. parishii</em> to characterize the genetic basis of hybrid incompatibility and examine its changing effects over multiple generations of experimental hybridization. In F<sub>2</sub>s, we found severe hybrid pollen inviability (< 50% reduction vs. parental genotypes) and pseudolinkage caused by a reciprocal translocation between Chromosomes 6 and 7 in the parental species. RILs retained excess heterozygosity around the translocation breakpoints, which caused substantial pollen inviability when interstitial crossovers had not created compatible heterokaryotypic configurations. Strong transmission ratio distortion and inter-chromosomal linkage disequilibrium in both F<sub>2</sub>s and RILs identified a novel two-locus genic incompatibility causing sex-independent gametophytic (haploid) lethality. The latter interaction eliminated three of the expected nine F<sub>2</sub> genotypic classes via F<sub>1</sub> gamete loss without detectable effects on the pollen number or viability of F<sub>2</sub> double heterozygotes. Along with the mapping of numerous milder incompatibilities, these key findings illuminate the complex genetics of plant hybrid breakdown and are an important step toward understanding the genomic consequences of natural hybridization in this model system.</p>
Supplement data for : Intratumoral drug-releasing microdevices allow in situ high throughput pharmaco phenotyping in patients with gliomas
<p>Transcriptomic and metabolomic data associated with the manuscript: Intratumoral drug-releasing microdevices allow in situ high throughput pharmaco phenotyping in patients with gliomas.</p> <p> </p>
Data from: Phenotypic plasticity and genetic diversity shed light on endemism of rare Boechera perstellata and its potential vulnerability to climate warming
<p>Premise of the study: The rapid pace of contemporary environmental change puts many species at risk, especially rare species constrained by limited capacity to adapt or migrate due to low genetic diversity and/or fitness. But the ability to acclimate can provide another way to persist through change. We compared the capacity of rare <em>Boechera perstellata</em> (Braun's rockcress) and widespread <em>B. laevigata</em> to acclimate to change.</p> <p>Methods: We investigated the phenotypic plasticity of growth, biomass allocation, and leaf morphology of individuals of <em>B. perstellata</em> and <em>B. laevigata</em> propagated from seed collected from several populations throughout their ranges in a growth chamber experiment to assess their capacity to acclimate. Concurrently, we assessed the genetic diversity of sampled populations using 17 microsatellite loci to assess evolutionary potential.</p> <p>Key results: Plasticity was limited in both rare <em>B. perstellata</em> and widespread <em>B. laevigata</em>, but differences in the plasticity of root traits between species suggest that <em>B. perstellata</em> may have less capacity to acclimate to change. In contrast to its widespread congener, <em>B. perstellata</em> exhibited no plasticity in response to temperature and weaker plastic responses to water availability. As expected, <em>B. perstellata</em> also had lower levels of observed heterozygosity than <em>B. laevigata</em> at the species level, but population-level trends in diversity measures were inconsistent due to high heterogeneity among <em>B. laevigata</em> populations.</p> <p>Conclusions: Overall, the ability of phenotypic plasticity to broadly explain the rarity of <em>B. perstellata</em> vs. commonness of <em>B. laevigata </em>is limited. However, some contextual aspects of our plasticity findings compared with its relatively low genetic variability may shed light on the narrow range and habitat associations of <em>B. perstellata</em> and suggest its vulnerability to climate warming due to acclimatory and evolutionary constraints.</p>
Data and R scripts associated with Clark, Moles, Fazlioglu, Brandenburger & Hartley, "Rapid loss of phenotypic plasticity in the introduced range of the beach daisy, Arctotheca populifolia."
<p>Data to accompany article accepted for publication in Journal of Ecology.</p> <p><strong>"Rapid loss of phenotypic plasticity in the introduced range of the beach daisy, <em>Arctotheca populifolia"</em></strong></p> <p>By Charlie D. Clark, Angela T. Moles, Fatih Fazlioglu, Claire R. Brandenburger, & Stephen Hartley</p> <p>1 zip file that contains the following:</p> <p> 2 datasets (xlsx format)</p> <p> 9 R scripts to run the analyses and produce figures</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.