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Supplementary material 1 from: Yi P, Yu P, Liu J, Xu H, Liu X (2018) A DNA barcode reference library of Neuroptera (Insecta, Neuropterida) from Beijing. ZooKeys 807: 127-147. https://doi.org/10.3897/zookeys.807.29430
Figure S1. Photographs of male genitalia of species of Coniopterygidae newly recorded from Beijing : Explanation note: A. Conwentziasinica Yang, 1974; B. Semidalisbicornis Liu & Yang, 1993. Scale bar 0.5 mm.
Supplementary material 2 from: Yi P, Yu P, Liu J, Xu H, Liu X (2018) A DNA barcode reference library of Neuroptera (Insecta, Neuropterida) from Beijing. ZooKeys 807: 127-147. https://doi.org/10.3897/zookeys.807.29430
Figure S2. Photographs of male genitalia of species of Chrysopidae newly recorded from Beijing : Explanation note: A. Nothochrysasinica Yang, 1986; B. Chrysopaintima McLachlan, 1893; C. Chrysoperlafurcifera (Okamoto, 1914); D. Chrysopidiaciliata (Wesmael, 1841); E. Pseudomalladacognatellus (Okamoto, 1914); F. Pseudomalladaqinlingensis (Yang & Yang, 1989); G. Ninetagrandis Navás, 1915. Scale bar: 0.5 mm.
Figure 1 in A new taxonomist-curated reference library of DNA barcodes for Neotropical electric fish (Teleostei: Gymnotiformes)
Figure 1. Percentages of 934 BOLD systems CO1 sequences (A) and 1015 GenBank CO1 sequences (B) categorized with respect to comparisons with reference library sequences. Minimum interspecific pairwise genetic divergence thresholds of 1.0% and 2.0% distinguish conspecifics (≤1.0/2.0%) and heterospecifics (>1.0/2.0%).
Fig. 7 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 7. Levels of major glucosinolates in leaves of first year rosette plants of the Gtype (A) and P-type (B) of Barbarea vulgaris in plants subjected to various challenges or no challenge as control. The contrasting general profile of the types is evident from dominance of BAR in the G-type and EBAR in the P-type. Treatment codes are: Control, un-challenged plants harvested after 7 days; Pieris 3d and Pieris 7d, herbivory by Pieris brassicae larvae until harvest at either day 3 or day 7; Plutella, herbivory by Plutella xylostella for 4 days; CuCl2, spraying of leaves with 10 mM CuCl2 (aq.) followed by recovery for 4 days. Bars represent means, whiskers indicate standard deviation (N = 3 for each group).
Fig. 6 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 6. Lack of the aliphatic glucosinolate (GSL) Pren (107) in B. vulgaris and spiking of pure (intact) Pren for establishing the limit of detection. A. Total ion chromatogram for the three dominating peaks in G-type B. vulgaris (dGSL preparation). B. Extracted ion trace for desulfo Pren in the same extract as A, showing lack of detection. C, D, E. Results of serial spiking of the crude extract with serial 10-fold dilutions of Pren before the desulfation procedure, showing linearity also at low levels and ability to detect trace levels.
Fig. 9 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 9. Extracted ion HPLC-MS chromatograms of desulfoglucosinolates prepared from glucosinolates (GSLs) in seeds (A–F) or leaves (G) of Reseda luteola and seeds of Reseda odorata (H). The three major peaks (A, B, C) represent PE, IM and BAR, much like many Barbarea spp. Focus on minor peaks (D) allowed conclusive identification of EBAR, confirmed by tR and the characteristic MS2 spectrum. A range of putative derivatives were not detected (E, F), but an unidentified hydroxybutylGSL was present (G), as was a known glycoside in R. odorata.
Fig. 5 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 5. MS2 spectra of desulfated glucosinolates (GSLs) confirming the identity of two 2-3homoMet-derived GSLs in C. hirsuta. While the spectrum of desulfo Buen contains only the usual fragments for this type of dGSL (a, [anhydroGlc+Na]+; b, [thioGlc+Na]+), the spectrum of desulfo Peen contains an additional usual fragment (c, [M-anhydroGlc Na]+) and two unusual + fragment ions suggesting a structure-specific cyclization and exchange of O during fragmentation: 201, [gluconolactone Na]+ and 152, [C H NS Na]+. + 6 11 + The unusual fragments can be rationalized as fragment a plus O and fragment c minus O, respectively.
Fig. 3 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 3. HPLC-MS chromatograms of desulfoglucosinolates (dGSLs) prepared from glucosinolates (GSLs) in Planodes virginica (A) and Nasturtium officinale (B–C) seeds, showing qualitative similarities and quantitative contrasts. Major peaks (B) from N. officinale revealed many of the same GSLs as in A, but levels of EBAR (40R) were much lower while levels were much higher for the biosynthetic precursor PE (105). A focus on trace peaks from N. officinale (C) revealed sharp peaks representing a range of minor constituents. Due to the closely eluting peaks, the latter chromatograms (B–C) were made by combining extracted ion chromatograms corresponding to [M+Na]+ of the indicated dGSLs. In C, the m/z 366 signal of d105 was omitted to allow visualization of minor coeluting peaks. An asterisk after a peak number indicates tentative identification. HPLC-MS conditions as in Olsen et al. (2016). TIC, total ion chromatogram, EIC, extracted ion chromatogram.
Fig. 4 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 4. HPLC-MS chromatogram of desulfoglucosinolates (dGSLs) prepared from glucosinolates (GSLs) from leaves (A) and petioles (B) of horseradish (Armoracia rusticana), focusing on trace level GSLs. The chromatograms were made by combining extracted ion chromatograms corresponding to [M+Na]+ of the indicated dGSLs, from analyses that were much overloaded with respect to the dominating dGSL d107 from Pren. In panel A, an insert shows magnification of the chromatogram from 5.2 to 5.8 min. Neither suggested BAR nor EBAR were detectable. In panel B, only extracted ion chromatograms of m/z 382 (BAR/EBAR), 352 (BZ), 366 (PE), 380 (3PP), 394 (4PB), 408 (5PP at high tR and 6mSOh at 5.4 min), 422 (7mSOh) and 436 (8mSOo) are included. Unlabeled trace peaks did not exhibit a combination of tR and m/z suitable for any of the mentioned candidates. HPLC conditions as in Olsen et al. (2016). Panel A depicts analysis of the Copenhagen garden accession; panel B from the naturalized population at Lake Fures¨o. An asterisk after a peak number indicates tentative identification.
Fig. 2 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 2. Detection of thioglucose-acylated glucosinolates (GSLs) by HPLC-MS of desulfated derivatives prepared from the indicated species. (A–C) Analysis of seeds of Arabidopsis thaliana Col-0 used as reference material for characteristic GSLs. Shown are the total ion chromatogram (A) and extracted ion chromatograms for sodium adducts of desulfo 6′Bz 4BzOb (d125) (B) and desulfo 6′Bz 4mSb (d127) (C). (D–F) Analysis of seeds of Barbarea grayi for dominating GSLs. Shown are total ion chromatograms (D), and extracted ion chromatograms for sodium adducts of desulfo 6'iF BAR (d131S) (E) and desulfo 6'iF PE (d129) (F).
Fig. 1 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 1. MS2 spectra of pairs of desulfoglucosinolates with and without a side chain double bond. Four short chain desulfoglucosinolates were investigated, including Na+ adducts of all (A–D) and in addition H+ adducts of the methylthio substituted (E–F), as indicated in each spectrum. The desulfo derivative of the putative 9mSn ([89]), poorly characterized in the literature, was also investigated (G).
Data from: Untangling taxonomy: a DNA barcode reference library for Canadian spiders
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Data from: DNA barcoding fishes from the Congo and the Lower Guinean provinces: assembling a reference library for poorly inventoried fauna
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Data from: Starting a DNA barcode reference library for shallow water polychaetes from the southern European Atlantic coast
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Fig 6 in A checklist of the bats of Peninsular Malaysia and progress towards a DNA barcode reference library
Fig 6. Neighbour-joining tree showing all available DNA barcodes for species in family Vespertilionidae reported from Peninsular Malaysia. The percentage of pseudoreplicate trees (±70%) in which the DNA barcodes clustered together in the bootstrap test (500 pseudoreplicates) are shown above the branches. Abbreviation as follows: PM = Peninsular Malaysia, VN = Vietnam, BN = Borneo (including Sabah & Sarawak of East Malaysia, Brunei and Kalimantan Indonesia), TH = Thailand, LA = Laos, SM = Sumatera Indonesia, JV = Java Indonesia, CH = China, CM = Cambodia. https://doi.org/10.1371/journal.pone.0179555.g006
Fig 1 in A checklist of the bats of Peninsular Malaysia and progress towards a DNA barcode reference library
Fig 1. Bat species with recent (dated during or after the year 2000) and old (dated before year 2000) records from Peninsular Malaysia. https://doi.org/10.1371/journal.pone.0179555.g001
Supplementary material 6 from: Yi P, Yu P, Liu J, Xu H, Liu X (2018) A DNA barcode reference library of Neuroptera (Insecta, Neuropterida) from Beijing. ZooKeys 807: 127-147. https://doi.org/10.3897/zookeys.807.29430
File S2. Checklist of the species of Neuroptera from Beijing :
Supplementary material 5 from: Yi P, Yu P, Liu J, Xu H, Liu X (2018) A DNA barcode reference library of Neuroptera (Insecta, Neuropterida) from Beijing. ZooKeys 807: 127-147. https://doi.org/10.3897/zookeys.807.29430
File S1. List of all specimens used in this study, including GenBank accession numbers :
Figure 1 from: Yi P, Yu P, Liu J, Xu H, Liu X (2018) A DNA barcode reference library of Neuroptera (Insecta, Neuropterida) from Beijing. ZooKeys 807: 127-147. https://doi.org/10.3897/zookeys.807.29430
Figure 1 Neighbor-joining tree based on the COI sequence dataset of the lacewing species from Beijing. Different color of clades represents different species.
Figure 4 from: Yi P, Yu P, Liu J, Xu H, Liu X (2018) A DNA barcode reference library of Neuroptera (Insecta, Neuropterida) from Beijing. ZooKeys 807: 127-147. https://doi.org/10.3897/zookeys.807.29430
Figure 4 Habitus photographs of species of Hemerobiidae newly recorded from Beijing. ADrepanepteryxalgida (Erichson, 1851) BHemerobiusbispinus Banks, 1940 CHemerobiusexoterus Navás, 1936 DHemerobiushumulinus Linnaeus, 1758 EHemerobiusjaponicus Nakahara, 1915 FHemerobiusmarginatus (Stephens, 1836) GHemerobiussubtriangulus Yang, 1987 HSympherobiusmanchuricus Nakahara, 1960. Scale bar: 1 mm.
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