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209 results for “scanning electron microscopy”
Text-fig. 22. Scanning electron microscope (SEM, a–c) and synchrotron radiation X-ray tomographic microscopy (SRXTM, d) images of Ibericarpus cuneiformis gen. et sp. nov.; Catefica locality, Portugal. a) Fruiting axis bearing an elongated receptacle with numerous diamond-shaped scars from detached fruitlets; note the absence of scars from bracts, tepals or stamens at the transition to the fruitlet scars and the stalk (arrow); b) Group of ten fruitlets detached from fruiting axis in (a) showing apical stigmatic region and distinctive bulging isodiametric epidermal cells; c) Detached fruitlet showing apical stigmatic region; d) Volume rendering of three adhering fruits showing apical stigmatic region and distinctive bulging isodiametric epidermal cells. Specimens, Catefica MM75-P0477 (a, b), Catefica 49-S115852 (c), Catefica 50-S174907 (d). Scale bars = 300 Μm (a–d). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 22. Scanning electron microscope (SEM, a–c) and synchrotron radiation X-ray tomographic microscopy (SRXTM, d) images of Ibericarpus cuneiformis gen. et sp. nov.; Catefica locality, Portugal. a) Fruiting axis bearing an elongated receptacle with numerous diamond-shaped scars from detached fruitlets; note the absence of scars from bracts, tepals or stamens at the transition to the fruitlet scars and the stalk (arrow); b) Group of ten fruitlets detached from fruiting axis in (a) showing apical stigmatic region and distinctive bulging isodiametric epidermal cells; c) Detached fruitlet showing apical stigmatic region; d) Volume rendering of three adhering fruits showing apical stigmatic region and distinctive bulging isodiametric epidermal cells. Specimens, Catefica MM75-P0477 (a, b), Catefica 49-S115852 (c), Catefica 50-S174907 (d). Scale bars = 300 Μm (a–d).
Text-fig. 33. Synchrotron radiation X-ray tomographic microscopy (SRXTM, a, c) and scanning electron microscope (SEM, b, d) images of Paisia pantoporata (a–c) and?Paisia sp. (d); Catefica locality, Portugal. a) Lateral view (volume rendering) of flower showing the carpels (c) and the fleshy tepals (t) that have a slightly bulge near the base; b) Pollen grains in situ from stamen showing scattered pores and spiny supratectal ornamentation; c) Transverse section (orthoslice xz1024) through flower showing the pentamerous organization with five tepals (green) five stamens (yellow) and five carpels (red) all on the same radii; d) Lateral view of floral structure with three free carpels borne on the swollen receptacle that has poorly defined facets at the apex indicating the former presence of perianth parts. Specimens, Catefica 49-S101214 (a, c), Catefica 50-S170188 (b), Catefica MM125-P0292 (d). Scale bars = 300 Μm (a, c, d), 6 Μm (b). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 33. Synchrotron radiation X-ray tomographic microscopy (SRXTM, a, c) and scanning electron microscope (SEM, b, d) images of Paisia pantoporata (a–c) and?Paisia sp. (d); Catefica locality, Portugal. a) Lateral view (volume rendering) of flower showing the carpels (c) and the fleshy tepals (t) that have a slightly bulge near the base; b) Pollen grains in situ from stamen showing scattered pores and spiny supratectal ornamentation; c) Transverse section (orthoslice xz1024) through flower showing the pentamerous organization with five tepals (green) five stamens (yellow) and five carpels (red) all on the same radii; d) Lateral view of floral structure with three free carpels borne on the swollen receptacle that has poorly defined facets at the apex indicating the former presence of perianth parts. Specimens, Catefica 49-S101214 (a, c), Catefica 50-S170188 (b), Catefica MM125-P0292 (d). Scale bars = 300 Μm (a, c, d), 6 Μm (b).
Text-fig. 2. Scanning electron microscope (SEM, a, c–f) and synchrotron radiation X-ray tomographic microscopy (SRXTM, b, g) images of the flower of Mugideiriflora portugallica (a, b) and fruits, seeds and pollen of Canrightia resinifera (c–g); Catefica locality, Portugal. a) Oblique, apical view of flower showing multiparted organization with numerous laminar tepals, stamens that are rhomboidal and flattened in transverse section and carpels that are borne on the short conical apex of the receptacle; b) Transverse section (orthoslice xy0800) through basal part of flower showing the elongate bases of the laminar tepals and the flattened rhomboidal bases of the stamens; c) Fruit in lateral view showing irregular surface resulting from the abundant resin bodies in the fruit and hypanthium wall, scars from stamens on the rim of the hypanthium (arrowheads) and the lobed apical stigmatic region (st); d) Broken fruit with one or two seeds missing but showing three pendant, orthotropous seeds with pointed micropylar regions (mi) and a finely pitted crystalliferous endotesta; note the remains of the apical vascular bundles (vb); e) Single seed isolated from a fruit showing two distinct bundles (vb) still attached apically to the chalazal region of the seed, the pointed micropyle (mi) and the finely pitted surface of the crystalliferous endotesta; f) Monocolpate pollen from stigmatic region of fruit showing the long colpus and coarse reticulum; g) Transverse section (orthoslice xy0705) through a fruit showing four seeds all with radially elongated endothelium cells formed from the inner epidermis of the tegmen (asterisks). Specimens, Catefica 150-S174254 (a, b), Catefica 49-S170377 (c), Catefica 49-S170372 (d), Catefica 50-S170401 (e), Catefica 50-S170404 (f), Catefica 50-S174906 (g). Scale bars = 300 Μm (a–e, g), 6 Μm (f). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 2. Scanning electron microscope (SEM, a, c–f) and synchrotron radiation X-ray tomographic microscopy (SRXTM, b, g) images of the flower of Mugideiriflora portugallica (a, b) and fruits, seeds and pollen of Canrightia resinifera (c–g); Catefica locality, Portugal. a) Oblique, apical view of flower showing multiparted organization with numerous laminar tepals, stamens that are rhomboidal and flattened in transverse section and carpels that are borne on the short conical apex of the receptacle; b) Transverse section (orthoslice xy0800) through basal part of flower showing the elongate bases of the laminar tepals and the flattened rhomboidal bases of the stamens; c) Fruit in lateral view showing irregular surface resulting from the abundant resin bodies in the fruit and hypanthium wall, scars from stamens on the rim of the hypanthium (arrowheads) and the lobed apical stigmatic region (st); d) Broken fruit with one or two seeds missing but showing three pendant, orthotropous seeds with pointed micropylar regions (mi) and a finely pitted crystalliferous endotesta; note the remains of the apical vascular bundles (vb); e) Single seed isolated from a fruit showing two distinct bundles (vb) still attached apically to the chalazal region of the seed, the pointed micropyle (mi) and the finely pitted surface of the crystalliferous endotesta; f) Monocolpate pollen from stigmatic region of fruit showing the long colpus and coarse reticulum; g) Transverse section (orthoslice xy0705) through a fruit showing four seeds all with radially elongated endothelium cells formed from the inner epidermis of the tegmen (asterisks). Specimens, Catefica 150-S174254 (a, b), Catefica 49-S170377 (c), Catefica 49-S170372 (d), Catefica 50-S170401 (e), Catefica 50-S170404 (f), Catefica 50-S174906 (g). Scale bars = 300 Μm (a–e, g), 6 Μm (f).
Text-fig. 20. Synchrotron radiation X-ray tomographic microscopy (SRXTM, a) and scanning electron microscope (SEM, b–h) images of fruits of Appomattoxia sp. (a–d) and anther and pollen of Goczania rugosa (e–h); Catefica locality, Portugal. a) Surface rendering of fruit in lateral view showing densely spaced hairs, some with delicate coiled tips; b) Fruit in lateral view showing short, densely spaced hairs and apical stigmatic region; c, d) Detail of fruit surface and hairs from fruit in (b); e) Fragmentary anther showing four pollen sacs; f) Proximal view of pollen grains from an abraded anther showing microechinate surface of pollen wall and clusters of small, spiny orbicules; g, h) Proximal (g) and distal (h) views of pollen grains from an isolated pollen sac, showing short colpus (h), tectate pollen wall and microechinate surface ornamentation. Specimens, Catefica 49-S174913 (a), Catefica 49-S107794 (b–d), Catefica 50-S170391 (e), Catefica 49-S170138 (f), Catefica 49-S170143 (g, h). Scale bars = 300 Μm (a, b, e), 100 Μm (c), 50 Μm (d), 6 Μm (f–h). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 20. Synchrotron radiation X-ray tomographic microscopy (SRXTM, a) and scanning electron microscope (SEM, b–h) images of fruits of Appomattoxia sp. (a–d) and anther and pollen of Goczania rugosa (e–h); Catefica locality, Portugal. a) Surface rendering of fruit in lateral view showing densely spaced hairs, some with delicate coiled tips; b) Fruit in lateral view showing short, densely spaced hairs and apical stigmatic region; c, d) Detail of fruit surface and hairs from fruit in (b); e) Fragmentary anther showing four pollen sacs; f) Proximal view of pollen grains from an abraded anther showing microechinate surface of pollen wall and clusters of small, spiny orbicules; g, h) Proximal (g) and distal (h) views of pollen grains from an isolated pollen sac, showing short colpus (h), tectate pollen wall and microechinate surface ornamentation. Specimens, Catefica 49-S174913 (a), Catefica 49-S107794 (b–d), Catefica 50-S170391 (e), Catefica 49-S170138 (f), Catefica 49-S170143 (g, h). Scale bars = 300 Μm (a, b, e), 100 Μm (c), 50 Μm (d), 6 Μm (f–h).
Text-fig. 19. Synchrotron radiation X-ray tomographic microscopy (SRXTM, a–c) of Aristospermum huberi and scanning electron microscope (SEM, d, e) images of Choffaticarpus compactus; Catefica locality, Portugal. a) Volume rendering of strongly flattened, triangular seed with pointed micropylar region; note thin-walled cells of outer integument preserved along the margins of the seed and pitted surface of the crystalliferous inner cells of outer integument where the outer cells are abraded and the narrow, lateral funicle/raphe; b) Volume rendering of seed showing surface of inner integument (endotesta) with cells showing clear imprints of crystals (arrows); c) Longitudinal section (orthoslice yz0241) of seed showing crystalliferous cells of endotesta (white arrows) and the two fiber layers of the tegmen that are perpendicular to each other (inner integument, ii-f, black arrows); d) Fragment of multiparted, apocarpous fruiting structure showing several helically-arranged, laterally flattened, fruitlets; e) Fruitlet in lateral view showing the prominent ventral face with its lateral groove, short attachment scar, and sunken regions of the fruit wall that indicate the probable presence of oil cells. Specimens, Catefica 49-S266049 (a–c), Catefica 49-S172558 (d), Catefica 49-S118675 (e). Scale bars = 300 Μm (a, c–e), 100 Μm (b). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 19. Synchrotron radiation X-ray tomographic microscopy (SRXTM, a–c) of Aristospermum huberi and scanning electron microscope (SEM, d, e) images of Choffaticarpus compactus; Catefica locality, Portugal. a) Volume rendering of strongly flattened, triangular seed with pointed micropylar region; note thin-walled cells of outer integument preserved along the margins of the seed and pitted surface of the crystalliferous inner cells of outer integument where the outer cells are abraded and the narrow, lateral funicle/raphe; b) Volume rendering of seed showing surface of inner integument (endotesta) with cells showing clear imprints of crystals (arrows); c) Longitudinal section (orthoslice yz0241) of seed showing crystalliferous cells of endotesta (white arrows) and the two fiber layers of the tegmen that are perpendicular to each other (inner integument, ii-f, black arrows); d) Fragment of multiparted, apocarpous fruiting structure showing several helically-arranged, laterally flattened, fruitlets; e) Fruitlet in lateral view showing the prominent ventral face with its lateral groove, short attachment scar, and sunken regions of the fruit wall that indicate the probable presence of oil cells. Specimens, Catefica 49-S266049 (a–c), Catefica 49-S172558 (d), Catefica 49-S118675 (e). Scale bars = 300 Μm (a, c–e), 100 Μm (b).
Dataset related to article "Imaging the kidney with an unconventional scanning electron microscopy technique: analysis of the subpodocyte space in diabetic mice".
<p><strong>Datasets:</strong></p> <p><strong>Table 1_Systemic parameters.xlsx </strong>- dataset related to the systemic parameters. These data are presented in Table 1. </p> <p><strong>Figure 6_Morphometric analysis.xlsx </strong>- dataset related to the morphometric characterization of the subpodocyte space and podocytes. These data are presented in Figure 6. </p> <p><strong>Abstract of the manuscript</strong></p> <p>Transmission electron microscopy (TEM) remains the gold standard for renal histopathological diagnoses, given its higher resolving power compared to light microscopy. However, it imposes several limitations on pathologists, including longer sample preparation time and a small observation area. To overcome these, we introduced a scanning electron microscopy (SEM) technique for imaging resin-embedded semi-thin sections of renal tissue. We developed a rapid tissue preparation protocol for experimental models and human biopsies which, alongside SEM digital imaging acquisition of secondary electrons (SE-SEM), enables fast electron microscopy examination, with a resolution similar to that achieved by TEM. We used this unconventional SEM imaging approach to investigate the subpodocyte space (SPS) in BTBR <em>ob/ob</em> mice with type 2 diabetes. Analysis of semi-thin sections with secondary electrons revealed that the SPS had expanded in volume and covered large areas of the glomerular basement membrane, forming wide spaces between the podocyte body and underlying filtering membrane. Our results show that SE-SEM is a valuable tool for imaging the kidney at the ultrastructural level, filling the magnification gap between light microscopy and TEM, and reveal that in diabetic mice the SPS is larger than in normal controls, which is associated with podocyte damage and impaired kidney function.</p>
Dataset of "Challenging Point Scanning across Electron Microscopy and Optical Imaging using Computational Imaging"
<p>Dataset containing the jupyter notebook with codes for the simulation of the structured illumination patterns used for image reconstruction (the simulation parameters have been optimized to make sure that the patterns were almost identical to the experimental ones), the reconstruction algorithms. Moreover, there are three experimental dataset saved as txxt file, where each line contains the six biases applied to the electron modulator and the intensity measured by the single pixel detector that we used.</p>
Dataset of "Near-real-time diagnosis of electron optical phase aberrations in scanning transmission electron microscopy using an artificial neural network"
<p>Dataset containing the jupyter notebook used to construct the database of image, to model and train ANN and to analyze the experimental data. Furthermore there are also a reduced database of 100 images that can be utilized to test the ANN, the h5 file containing the ANN weigths and other supporting files.</p>
Figs 11–22 in Palp sensory structures in adult caddisflies of the suborder Annulipalpia (Trichoptera): a scanning electron microscopy study
Figs 11–22. Palp sensilla of caddisflies of the suborder Annulipalpia: 11 – N. bimaculata
Figs 7–10 in Palp sensory structures in adult caddisflies of the suborder Annulipalpia (Trichoptera): a scanning electron microscopy study
Figs 7–10. Labial palps of P. apicalis (7–8) and N. bimaculata (9–10) females. 7 – first
Figs 1–6 in Palp sensory structures in adult caddisflies of the suborder Annulipalpia (Trichoptera): a scanning electron microscopy study
Figs 1–6. Medial (1–5) and ventrolateral (6) surfaces of maxillary palp of D. robusta
Data from: Crustacean photoreceptor damage and recovery: Applying a novel scanning electronic microscopy protocol in artificial light at night studies
<p>As sources of artificial light at night (ALAN) expand worldwide, research on their impacts has also increased. Most of these studies, including those in coastal habitats, have focused on behavioral and ecological responses to ALAN, overlooking impacts on the photoreceptor, the basic functional structure of animals to absorb light. Examining structural changes in the photoreceptor is essential to understand the mechanisms by which ALAN may be impacting species, particularly those adapted to different light backgrounds. This study examined the photoreceptor (rhabdom) of two sandy beach crustaceans exhibiting different light tolerances at night: the amphipod <em>Orchestoidea tuberculata</em> and the isopod <em>Tylos spinulosus</em>. We developed a novel protocol to measure these species' photoreceptor areas and quantify the damage caused by ALAN using histological sections and scanning electron microscopy (SEM). Our results showed that in the isopod, a species naturally adapted to lower light intensities at night than the amphipod, the rhabdom surface was 20-times larger, and presented a tapetum, an adaptive feature found in species living in low light conditions. This confirmed that this species is potentially more sensitive to ALAN than the amphipod. Consistently, a brief period of exposure to ALAN (1 h, 20 lux) caused 3-6 times more damage in the isopod' rhabdom. In fact, ALAN caused structural damage in the isopod' but not in the amphipod' rhabdom, a damage that did not show signs of recovery from ALAN after 1 and 24 h. Thus, the damage caused by ALAN on an organism's photoreceptors is likely to be more severe and persistent in species naturally adapted to lower light levels at night. Installation of permanent ALAN sources nearby the burrowing area of these light sensitive species may have differential effects on their activity and interactions at night. ALAN may also become a new selection pressure on these species, a concern with wide implications given the ubiquity among animals of the photoreceptor structure and its response to light.</p>
Correlative Raman Imaging and Scanning Electron Microscopy: The Role of Single Ga Islands in Surface-Enhanced Raman Spectroscopy of Graphene_experimental dataset
<p>This dataset contains the raw unprocessed data for Piastek et al., Correlative Raman Imaging and Scanning Electron Microscopy: The Role of Single Ga Islands in Surface-Enhanced Raman Spectroscopy of Graphene, <em>J. Phys. Chem. C</em> 2022, 126, 9, 4508–4514. </p>
Supplemental Material to Journal Article "Determination of as-built properties of fiber reinforced polymers in a wind turbine blade using scanning electron and high-resolution X-ray microscopy"
<p>This set supplements the figure data to the article "Determination of as-built properties of fiber reinforced polymers in a wind turbine blade using scanning electron and high-resolution X-ray microscopy", DOI: <a href="https://doi.org/10.1016/j.jcomc.2022.100310">https://doi.org/10.1016/j.jcomc.2022.100310</a></p>
Supplementary material for: Sparse Arrays for Four-Dimensional Scanning Transmission Electron Microscopy
<p>Supplementary material for: Sparse Arrays for Four-Dimensional Scanning Transmission Electron Microscopy</p> <p>The link to the main publication with detailed information will be added later.</p> <p>The three videos show liv eand offline processing with the CheeTah T3, ASI Serval, ASI Accos, LiberTEM and CEOS Panta Rhei. They are also available at https://www.youtube.com/playlist?list=PLZCH_qD2RkB5oFdBc12xcYUcbmWZpJQHh</p> <p>benchmark.ipynb is a Jupyter notebook that was used to test the performance, as reported in the paper.</p> <p>raw_csr.zip is a test dataset recorded on a gold grid in the CSR format that can be opened by LiberTEM</p> <p>benchmark.ipynb is a Jupyter notebook that shows how the test dataset can be opened and processed with common Python packages and with LiberTEM.</p>
Scanning electron microscopy (SEM) images of single bacterial-Pb tolerant isolates
<p><strong>In-Vitro Trace Element Uptake by Bacteria in Liquid Cultures-Scanning Electron Microscopy </strong><strong>(SEM-EDX) Analysis</strong><br>Batch studies were conducted using 50 mL Falcon tubes that contained 25 mL GY rich medium supplemented with 100 mg L-1 of Pb (Pb(NO3)2). All tubes were inoculated with 1 mL of a bacterial cell suspension (optical density at 600 nm = 1), which was previously grown in GY medium at 28 °C for 24 h. Samples were incubated at 28 °C and 100 rpm on an orbital shaker.<br>In order to estimate Pb sequestration by bacterial cells, cultures were first centrifuged 15 min at 4800 rpm, bacterial pellets were washed 3 times with 0.01 M phosphate-buffer (PBS, pH 7.0) to remove unbound metals, sugars and proteins. The samples were dehydrated with ethanol/water mixtures, until absolute ethanol. Then 100% acetone was used. Pellets were resuspended in 2% glutaraldehyde for 1h at room temperature. Afterwards, bacterial samples were centrifuged for 3 min at 3000 rpm and pellets were washed 3 times with milli-Q water. One uL of sample was placed on a sample holder, in carbon conductive tape. Then, samples were coated 30 s with a 15 nm gold layer and analysed using a Scanning Electron Microscope (<span>Phenom™ ProX Desktop, Thermofisher</span>). Images were taken using an accelerating voltage of 15 kV.</p> <p><em>Bacillus paramycoides</em> ST4, <em>Bacillus wiedmannii</em> ST29 and <em>Bacillus proteolyticus </em>ST9 <span>images obtained by SEM-EDX are presented in this dataset. Pb was found present in/on the bacterial cell wall of these bacteria cultures and detected by EDX spectra. </span></p>
Dataset accompanying the publication "Towards 3D determination of the surface roughness of core-shell microparticles as a routine quality control procedure by scanning electron microscopy"
<p>This dataset accompanies the following publication:</p> <p>Hülagü, D., Tobias, C., Dao, R., Komarov, P., Rurack, K., Hodoroaba, V.-D., Towards 3D determination of the surface roughness of core-shell microparticles as a routine quality control procedure by scanning electron microscopy. Sci.Rep, <span>14<span>, 17936 (2024), https://doi.org/10.1038/s41598-024-68797-7.</span></span></p> <p>It contains SEM and AFM-in-SEM images of polystyrene (PS) core particles, polystyrene-iron oxide (PS/Fe3O4) core-shell particles, and polystyrene-iron oxide-silica (PS/Fe3O4/SiO2) core-shell-shell particles. Please refer to the publication and its supporting information for more details on the acquisition and contents of the dataset, as well as the GitHub repository at https://github.Com/denizhulagu/roughness-analysis-by-electron-microscopy.</p> <p> </p> <p>The investigated particles were produced at BAM laboratories as previously described in:</p> <p>Hülagü, D. et al. Generalized analysis approach of the profile roughness by electron microscopy with the example of hierarchically grown polystyrene–iron oxide–silica core–shell–shell particles. Adv. Eng. Mater. 24, 2101344, https://doi.org/10.1002/adem.202101344 (2022).</p> <p>Tobias, C., Climent, E., Gawlitza, K. & Rurack, K. Polystyrene microparticles with convergently grown mesoporous silica shells as a promising tool for multiplexed bioanalytical assays. ACS Appl. Mater. Interfaces 13, 207, https://dx.doi.org/10.1021/acsami.0c17940 (2020).</p>
Scanning electron microscopy (SEM) images of particulate matter collected on air filters
<p>Airborne PM sampling was conducted within a larger study on the PM composition of different areas in Santa Rosa, La Pampa, Argentina by Prof. Dr. Mendez Mariano. Airborne PM10 samples were collected on commercial 47mm diameter PTFE membrane filters (Image 1-blank) and Nylon filters (Image 2-blank). The PM10 was collected using an electrostatic precipitator coupled with the Easy Dust Generator (EDG). Filters were analysed using a Scanning Electron Microscope (Phenom™ ProX Desktop, Thermofisher). Images were taken using an accelerating voltage of 15 kV. SEM-EDX results are presented in this dataset.</p>
Serial block-face scanning electron microscopy of adherent cells on thin plastic substrate – Data set 10
<p>Serial block-face (SBF) scanning electron microscopy (SEM) is used for imaging the entire internal ultrastructure of cells, tissue samples or small organisms. We developed a workflow for SBF SEM of adherent cells, such as <em>Giardia</em> parasites and HeLa cells, attached to the surface of a plastic culture dish, which preserves the interface between cells and plastic substrate. Cells were embedded <em>in situ</em> on their substrate using silicone microwells and were mounted for cross-sectioning which allowed SBF imaging of large volumes and many cells. In total we provide 10 data sets with image series from SBF SEM of <em>Giardia</em> and HeLa cells prepared with protocol variants to improve the workflow. A detailed description of the methods and the data set is provided in the download container.</p> <p>Data set 10 comprises an image series of 255 images recorded of a HeLa cell adhered to the plastic substrate of a culture dish. SBF SEM was done at a section interval of 50 nm using the T1 detector of the SEM at high vacuum. Original pixel size was 10 nm. The data folder contains the raw image files, processed image files (see data set description for details of the processing), a video file of a processed image file series.</p>
Serial block-face scanning electron microscopy of adherent cells on thin plastic substrate – Data set 05
<p>Serial block-face (SBF) scanning electron microscopy (SEM) is used for imaging the entire internal ultrastructure of cells, tissue samples or small organisms. We developed a workflow for SBF SEM of adherent cells, such as <em>Giardia</em> parasites and HeLa cells, attached to the surface of a plastic culture dish, which preserves the interface between cells and plastic substrate. Cells were embedded <em>in situ</em> on their substrate using silicone microwells and were mounted for cross-sectioning which allowed SBF imaging of large volumes and many cells. In total we provide 10 data sets with image series from SBF SEM of <em>Giardia</em> and HeLa cells prepared with protocol variants to improve the workflow. A detailed description of the methods and the data set is provided in the download container.</p> <p>Data set 05 comprises an image series of 500 images recorded of a HeLa cell adhered to the plastic substrate of a culture dish. SBF SEM was done at a section interval of 50 nm using the DBS detector of the SEM at low vacuum (0.4 mbar). Original pixel size was 6 nm. The data folder contains the raw image files, processed image files (see data set description for details of the processing), a video file of a processed image file series.</p>
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.