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Dataset results
71 results for “simulated microgravity”
T Cell Transcriptome Alterations in Healthy Volunteers Exposed to Three Weeks of Simulated Microgravity
GEO Series GSE301964. Homo sapiens. 50 samples. Type: Expression profiling by high throughput sequencing.
Simulated Microgravity Inhibits Differentiation and Alters Gene Expression Profiles of 2T3 Pre-osteoblasts
GEO Series GSE1367. Mus musculus. 6 samples. Type: Expression profiling by array.
Transcriptional profiling of the probiotic E. coli Nissle 1917 strain under simulated microgravity and normal gravity
GEO Series GSE147272. Escherichia coli. 8 samples. Type: Expression profiling by high throughput sequencing.
Single Cell Analysis Identifies Conserved Features of Immune Dysfunction in Simulated Microgravity and Spaceflight [scRNA-Seq]
GEO Series GSE218936. Homo sapiens. 8 samples. Type: Expression profiling by high throughput sequencing.
Effect of simulated microgravity on E. coli K12 MG1655 growth and gene expression
GEO Series GSE40648. Escherichia coli; Escherichia coli str. K-12 substr. MG1655. 8 samples. Type: Expression profiling by array.
Effects of an aged tissue niche on the immune potency of dendritic cells using simulated microgravity
GEO Series GSE232153. Homo sapiens. 24 samples. Type: Expression profiling by high throughput sequencing.
Enhanced self-renewal of human pluripotent stem cells by simulated microgravity
GEO Series GSE205559. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
A study of alterations in DNA epigenetic modifications (5mC and 5hmC) and gene expression influenced by simulated microgravity in human lymphoblastoid cells
GEO Series GSE65944. Homo sapiens. 8 samples. Type: Methylation profiling by high throughput sequencing; Expression profiling by high throughput sequencing.
A 3D Hydrogel Model to Assess Endothelial Cell-macrophage Interactions in Simulated Microgravity (OR-DRPD-SRI2019)
ClinicalTrials.gov study NCT04028752. IPD Sharing: NO. Countries: 0. Publications: 0.
Comparative Transcriptomic Signature of the Simulated Microgravity Response in C. elegans
GEO Series GSE122125. Caenorhabditis elegans. 15 samples. Type: Expression profiling by high throughput sequencing.
A study of gene expression influenced by simulated microgravity in human lymphoblastoid cells
Here in this study we systematically examined the patterns of DNA methylation and hydroxy-methylation with its functional implications in gene regulation for the cultured TK6 lymphoblastoid cells upon exposure to micro-gravity conditions. The results reported here indicate that simulated microgravity alters methylation patterns in a limited way and subsequently the expression of genes involved in stress response like ATF3 FBXO17 MAP3K13 and VCL in TK6 cells. Overall design: Examination of RNA-seq with 2 replicates each for 1 cell type
A study of alterations in DNA epigenetic modifications (5mC and 5hmC) influenced by simulated microgravity in human lymphoblastoid cells
Here in this study we systematically examined the patterns of DNA methylation and hydroxy-methylation with its functional implications in gene regulation for the cultured TK6 lymphoblastoid cells upon exposure to micro-gravity conditions. The results reported here indicate that simulated microgravity alters methylation patterns in a limited way and subsequently the expression of genes involved in stress response like ATF3 FBXO17 MAP3K13 and VCL in TK6 cells. Overall design: Examination of RNA-seq with 2 replicates each for 1 cell type
The influence of simulated microgravity on the proteome of Daphnia magna
Background/Objectives: The waterflea Daphnia is an interesting candidate for bioregenerative life support systems (BLSS). These animals are particularly promising because of their central role in the limnic food web and its mode of reproduction. However the response of Daphnia to altered gravity conditions has to be investigated especially on the molecular level to evaluate the suitability of Daphnia for BLSS in space. Methods: In this study we applied a proteomic approach to identify key proteins and pathways involved in the response of Daphnia to simulated microgravity generated by a 2D-clinostat. We analysed 5 biological replicates using 2D-DIGE proteomic analysis. Results: We identified 109 protein spots differing in intensity (p < 0.05). Substantial fractions of these proteins are involved in actin microfilament organisation indicating the disruption of cytoskeletal structures during clinorotation. Furthermore proteins involved in protein folding were identified suggesting altered gravity induced breakdown of protein structures in general. In addition simulated microgravity increased the abundance of energy metabolism related proteins indicating an enhanced energy demand of Daphnia. Conclusion: The affected biological processes were also described in other studies using different organisms and systems either aiming to simulate microgravity conditions or providing real microgravity conditions. Moreover most of the Daphnia protein sequences are well conserved throughout taxa indicating that the response to altered gravity conditions in Daphnia follows a general concept.
Streptococcus mutans differential gene expression in response to simulated microgravity
Astronauts have been previously shown to exhibit decreased salivary lysozyme and increased dental calculus and gingival inflammation in response to space flight host factors that could contribute to oral diseases such as caries and periodontitis. However the specific physiological response of caries-causing bacteria such as Streptococcus mutans to space flight and/or ground-based simulated microgravity has not been extensively investigated. In this study High Aspect Ratio Vessel (HARV) S. mutans simulated microgravity and normal gravity cultures were assessed for changes in metabolite and transcriptome profiles H2O2 resistance and competence in sucrose-containing biofilm media. Stationary phase S. mutans simulated microgravity cultures displayed increased killing by H2O2 compared to normal gravity control cultures but competence was not affected. RNA-seq analysis revealed that expression of 153 genes was up-regulated >= 2-fold and 94 genes down-regulated >= 2-fold during simulated microgravity HARV growth. These included a number of genes located on extrachromosomal elements as well as genes involved in carbohydrate metabolism translation and stress responses. Collectively these results suggest that growth under microgravity analog conditions promotes changes in S. mutans gene expression and physiology that may translate to an altered cariogenic potential of this organism during space flight missions. Overall design: Differential gene expression was compared between RNA from S. mutans grown in normal gravity HARVs (n=3 independent cultures) and RNA from S. mutans grown in simulated microgravity HARVs (n=3 independent cultures)
Global gene expression profiles of cardiac progenitors differentiated from human pluripotent stem cells in 3D culture under simulated microgravity
Methods: RNA-seq libraries were prepared using the Illumina TruSeq RNA kit and the TrueSeq method was employed for mRNA enrichment. The libraries were quantified and samples were multiplexed in each lane of the flowcell. Cluster generation was performed and then sequenced on the Illumina HiSeq1000 system. Reads were mapped on the Human Genome Reference and normalized expression table was generated. Results: Among differentially expressed genes 53 of them were up-regulated and 75 were down-regulated. Conclusions: Data demonstrate increased expression of genes associated with growth development and pro-survival in cardiac progenitors cultured under simulated microgravity compared with those cultured under standard gravity. RNA-sequencing analysis was performed to compare global gene expression profiles of cells at differentiation day 8 under simulated microgravity vs. standard gravity.
Global gene expression profiles of cardiac progenitors differentiated from human pluripotent stem cells in 3D culture under simulated microgravity
Methods: RNA-seq libraries were prepared using the Illumina TruSeq RNA kit and the TrueSeq method was employed for mRNA enrichment. The libraries were quantified and samples were multiplexed in each lane of the flowcell. Cluster generation was performed and then sequenced on the Illumina HiSeq1000 system. Reads were mapped on the Human Genome Reference and normalized expression table was generated. Results: Among differentially expressed genes 53 of them were up-regulated and 75 were down-regulated. Conclusions: Data demonstrate increased expression of genes associated with growth development and pro-survival in cardiac progenitors cultured under simulated microgravity compared with those cultured under standard gravity. RNA-sequencing analysis was performed to compare global gene expression profiles of cells at differentiation day 8 under simulated microgravity vs. standard gravity.
Transcription profiling of mouse osteoblasts under static vs simulated microgravity
The below table includes a smaller list of data that was analyzed by dChip and filtered by pvalue such that a file with about 4600 genes was obtained which allowed for ease of use from 40,000 genes. Experiment Overall Design: The total RNA was extracted from 2T3 pre-osteoblast cells exposed to static or simulated microgravity (Rotating Wall Vessel) conditions. The RNA was then sent to Affymetrix microarray core facility at Baylor College of Medicine (Houston TX) for microarray analysis.
Transcription profiling of Drosophila after exposure to microgravity in the International Space Station and in a microgravity simulator
Larvae-Pupae transition flies (Drosophila) were recovered and transport for 3 days at 12-14C to arrest development until the launch site then exposed to RT (18-20C) for some hours including the launch and trip to the International Space Station then pupae were exposed to microgravity in the ISS for 4 days and a half at 22C. Finally pupae were fixed on acetone and frozen until recovery on Earth. Four groups of samples: 1 ISS (+ground control) as described 2 RPM (microgravity simulator on Earth) as described 3 RPM without constrains (No MAMBA container and only 5 days exposure without cold transport) and 4 centrifuge 10g without constrains control.
Zebrafish larvae submitted to simulated microgravity on a clinostat (CLINO) at 5dpf for one day
The general objective of the study was to determine modulation of gene expression by environmental factors specifically simulation of microgravity with a special emphasis on bone formation. For this reason the specific period of treatment was chosen between 5-6 days post-fertilization (dpf) when bone formation and calcification are taking place. Zebrafish larvae were placed at 5 dpf into a clinostat (CLINO) for 24 hours which was shown to simulate microgravity by the specific rotational movements it generates. We show that CLINO exposure causes a clear decrease of bone formation as illustrated by cranial skeleton staining of the bone matrix by Alizarin Red by morphometric analysis of the resulting images. Thus a whole genome micro-array experiment was conducted to identify genes that may be involved in the observed effect on bone formation.
Effect of simulated microgravity on E. coli K12 MG1655 growth and gene expression
This study demonstrates simulated microgravity effects on E. coli K 12 MG1655 when grown on LB medium supplemented with glycerol. The results imply that E. coli readily reprograms itself to combat the multiple stresses imposed due to microgravity. Under these conditions it survives by upregulating oxidative stress protecting genes and simultaneously down regulating the membrane transporters and synthases to maintain cell homeostasis. In this study a clinostat that mimics microgravity conditions was used to investigate the effects of microgravity on E. coli grown in LB medium supplemented with glycerol to monitor the effects on growth and global gene expression using Affymetrix DNA microarrays.
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International Brain Laboratory public data
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OpenNeuro
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