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48 results for “soil bacterial community”
Data from: Life history determines biogeographical patterns of soil bacterial communities over multiple spatial scales
The extent to which the distribution of soil bacteria is controlled by local environment versus spatial factors (e.g., dispersal, colonisation limitation, evolutionary events) is poorly understood and widely debated. Our understanding of biogeographic controls in microbial communities is likely hampered by the enormous environmental variability encountered across spatial scales and the broad diversity of microbial life histories. Here we constrained environmental factors (soil chemistry, climate, above-ground plant community) to investigate the specific influence of space, by fitting all other variables first, on bacterial communities in soils over distances from m to 102 km. We found strong evidence for a spatial component to bacterial community structure that varies with scale and organism life history (dispersal and survival ability). Geographic distance had no influence over community structure for organisms known to have survival stages, but the converse was true for organisms thought to be less hardy. Community function (substrate utilisation) was also shown to be highly correlated to community structure, but not to abiotic factors, suggesting non-stochastic determinants of community structure are important Our results support the view that bacterial soil communities are constrained by both edaphic factors and geographic distance, and further show that the relative importance of such constraints depends critically on the taxonomic resolution used to evaluate spatio-temporal patterns of microbial diversity, as well as life-history of the groups being investigated, much as is the case for macro-organisms.
Fig. 1 in Review paper The Role of Bacterial-based Protist Communities in Aquatic and Soil Ecosystems and the Carbon Biogeochemical Cycle, with Emphasis on Naked Amoebae
Fig. 1. Protocol for respiration and microbial biomass experiments. Respiration was measured with an infrared CO gas analyzer, glu2 cose (444 µg/g soil) in solution was added as a supplement. Microbial assays for naked amoebae used a culture observation method (COM) for living amoebae routinely employed in our laboratory (e.g. Anderson 2000), glutaraldehyde-fixed portions of the soil sample were examined microscopically for bacteria and heterotrophic nanoflagellates using a fluorescent staining technique (Anderson et al. 2001). For experiments of one-week duration, sampling was done on Days 1, 4, and 8. For two-week duration experiments, sampling was done on Days 1, 8, and 15.
Data from: Life history determines biogeographical patterns of soil bacterial communities over multiple spatial scales
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Data from: Source-sink plasmid transfer dynamics maintain gene mobility in soil bacterial communities
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Data from: Positive selection inhibits gene mobilization and transfer in soil bacterial communities
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Data from: Secondary successional trajectories of structural and catabolic bacterial communities in oil polluted soil planted with hybrid poplar
Poplars have widely been used for rhizoremediation of a broad range of organic contaminants for the past two decades. Still, there is a knowledge gap regarding the rhizosphere associated bacterial communities of poplars and their dynamics during the remediation process. It is envisaged that a detailed understanding of rhizosphere associated microbial populations will greatly contribute to a better design and implementation of rhizoremediation. In order to investigate the long-term succession of structural and catabolic bacterial communities in oil polluted soil planted with hybrid poplar, we carried out a 2-year field study. Hybrid aspen (Populus tremula x tremuloides) seedlings were planted in polluted soil excavated from an accidental oil-spill site. Vegetated and un-vegetated soil samples were collected for microbial community analyses at 7 different time-points during the course of 2 years and sampling time-points were chosen to cover the seasonal variation in the boreal climate zone. Bacterial community structure was accessed by means of 16S rRNA gene amplicon pyrosequencing whereas catabolic diversity was monitored by pyrosequencing of alkane hydroxylase and extradiol dioxygenase genes. We observed a clear succession of bacterial communities on both structural and functional levels from early to late phase communities. Sphingomonas type extradiol dioxygenases and alkane hydroxylase homologs of Rhodococcus clearly dominated the early phase communities. The high dominance-low diversity functional gene communities underwent a transition to low-dominance-high diversity communities in the late phase. These results pointed towards increased catabolic capacities and a change from specialist to generalist strategy of bacterial communities during the course of secondary succession.
Data from: Secondary successional trajectories of structural and catabolic bacterial communities in oil polluted soil planted with hybrid poplar
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Impacts of invasive Australian acacias on soil bacterial community composition, microbial enzymatic activities, and nutrient availability in fynbos soils
<p>Invasive nitrogen-fixing plants often impact the soils they invade, notably through changes in soil chemistry and microbial community composition, which in turn could lead to alterations in soil functionality. We aimed to determine the impacts that invasive Australian <i>Acacia </i>trees have on soil chemistry and function (carbon, nitrogen, and phosphorus cycling) in South Africa's Core Cape Subregion, and whether any differences in soil function are linked to differences in soil chemical properties and bacterial community composition between neighbouring acacia-invaded and uninvaded sites. We do so by using Next Generation Sequencing (NGS) methods together with soil chemistry and soil enzyme functioning profiles. Acacias significantly increased levels of soil nitrogenous compounds (NO<sub>3</sub><sup>-</sup>, NH<sub>4</sub><sup>+</sup>, and total N), C, and pH. Acacias also significantly elevated microbial phosphatase activity, but not β-glucosidase, while having contrasting effects on urease. Acacia-induced changes in soil chemical properties were correlated with changes in enzyme activities for both urease and phosphatase. Changes in soil bacterial community composition due to the presence of invasive acacias were also correlated with phosphatase enzymatic activity. While we found evidence for acacias altering soil function by changing soil chemical properties and bacterial community composition, the legume-soil feedbacks underlying these impacts appear to be context specific.</p>
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Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.