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Figure 2 in Endemic and enigmatic: the reproductive biology of Aegla (Crustacea: Anomura: Aeglidae) with observations on sperm structure
Figure 2. Light micrographs of Aegla rostrata Jara, 1977. A, Spermatophoric lobes; B–G, Squash of spermatophoric lobe contents showing spermatozoa (arrowheads) scattered amongst assorted cells (Asterisk in E and G indicate individual spermatozoa with three microtubular arms).
Figure 1 in Endemic and enigmatic: the reproductive biology of Aegla (Crustacea: Anomura: Aeglidae) with observations on sperm structure
Figure 1. Occurrence of ovigerous females of Aegla species obtained from literature sources and USNM specimens. Total USNM specimens examined = 958 (40 ovigerous females). Symbols: 1 Bahamonde and López (1961); 2 Jara (1989); 3 Bueno and Bond-Buckup (2000); 4 Swiech-Ayoub and Masunari (2001a, 2001b); 5 Rodrigues and Hebling (1978); 6 Jara (1977); 7 Lopéz (1965). Shading = range from literature sources indicated. Solid black bar = number from USNM collection for that month.
Figure 3 in Endemic and enigmatic: the reproductive biology of Aegla (Crustacea: Anomura: Aeglidae) with observations on sperm structure
Figure 3. Transmission electron micrograph of spermatozoon of Aegla longirostri Bond-Buckup and Buckup, 1994 in longitudinal section. Abbreviations: av, acrosome vesicle; cy, cytoplasm; m, mitochondrion; ms, membrane system; mt, microtubular bundle; n, nucleus; p, perforatorial column; pm, periacrosomal material.
Fig. 1 in Different staining techniques evaluation for the study of sperm morphology and morphometry in bats (Mammalia: Chiroptera)
Fig. 1. Sperm morphology and morphometric variables. (a) Part of the spermatozoa, (b) morphometric variables measured in this study: head length (HL), head width (HW), middle piece length (MPL) and tail length (TL).
Fig. 2 in Different staining techniques evaluation for the study of sperm morphology and morphometry in bats (Mammalia: Chiroptera)
Fig. 2. Microscopic images of spermatozoa stained with (a) Toluidine Blue (TB), (b) Giemsa (G), (c) May Grünwald-Giemsa (MG-G), (d) GRAM (Gr), (e) Hematoxylin-Eosin (HE), (f) DAPI, (g) Janus Green and (JG) (h) Basic Fuchsin (BF), b=10 µm.
Fig. 3 in Sperm characteristics as additional evidence of close relationship between Lebiasina and Piabucina (Characiformes: Lebiasinidae: Lebiasininae)
Fig. 3. Spermatozoon of Lebiasina melanoguttata (a, b, c), L. aff. uruyensis1 (d, e, f) and L. aff. uruyensis2 (g, h, i). a, d, g: longitudinal sections. b, c, e, f, h, i: transverse sections from top to posterior region. The nucleus (n) of all species is dropshaped and slightly elongated towards the flagellar axis. The flagellum (f) lies lateral to the nucleus. The centriolar complex (p, d) and nuclear fossa (arrow) is superolateral. Note the presence of the striated rootlets (r) on opposite sides of the distal centriole (d). The proximal centriole (p) is slightly oblique relative to the distal centriole (d) (a-inset, d-inset, g-inset). The cytoplasmic canal is present (asterisk). The midpiece (pi) is short, asymmetrical, and contains the oblong mitochondria (m) and vesicles (v). a: axoneme, d: distal centriole, f: flagellum, m: mitochondria, n: nucleus, p: proximal centriole, r: striated rootlet, v: vesicle, asterisk: cytoplasmic canal, arrow: nuclear fossa. Bar = 0.5 µm.
Fig. 5 in Sperm characteristics as additional evidence of close relationship between Lebiasina and Piabucina (Characiformes: Lebiasinidae: Lebiasininae)
Fig. 5. Schematic representation of spermatozoa of Lebiasina and Piabucina. Both spermatozoa are very similar and share primarily the lateral nucleus, superolateral centriolar complex, striated rootlets, oblong mitochondria and some vesicles in the midpiece.
Fig. 4 in Sperm characteristics as additional evidence of close relationship between Lebiasina and Piabucina (Characiformes: Lebiasinidae: Lebiasininae)
Fig. 4. Spermatozoon of Piabucina boruca (a, b, c), P. elongata (d, e, f) and P. panamensis (g, h, i). a, d, g: longitudinal sections. b, c, e, f, h, i: transverse sections from top to posterior region. The nucleus (n) of all species is drop-shaped and slightly elongated towards the flagellar axis. The flagellum (f) lies lateral to the nucleus. The centriolar complex (p, d) and nuclear fossa (arrow) is superolateral. Note the presence of the striated rootlets (r) on opposite sides of the distal centriole (d). The proximal centriole (p) is slightly oblique relative to the distal centriole (d) (a-inset, d-inset, g-inset). The cytoplasmic canal is present (asterisk). The midpiece (pi) is short, asymmetrical, and contains the oblong mitochondria (m) and vesicles (v). a: axoneme, d: distal centriole, f: flagellum, m: mitochondria, n: nucleus, p: proximal centriole, r: striated rootlet, v: vesicle, asterisk: cytoplasmic canal, arrow: nuclear fossa. Bar = 0.5 µm.
Fig. 1 in Sperm characteristics as additional evidence of close relationship between Lebiasina and Piabucina (Characiformes: Lebiasinidae: Lebiasininae)
Fig. 1. Spermiogenesis process representative for Lebiasina and Piabucina. Figure subunits are longitudinal sections of spermatids corresponding to (a) Lebiasina aff. uruyensis1, (b) L. aff. uruyensis1, (c) Piabucina boruca, and (d) P. elongata. The flagellum is initially lateral to the nucleus. A slightly movement of the nucleus towards the flagellar axis is present. Consequently the centriolar complex and the nuclear fossa is superolateral. During spermiogenesis, the formation of two striated rootlets occurs on opposite sides of the distal centriole. c: cytoplasm, d: distal centriole, f: flagellum, n: nucleus, p: proximal centriole, pi: midpiece, r: striated rootlet, asterisk: cytoplasmic canal, arrow: nuclear fossa. Bar = 0.5 µm.
Fig. 2 in Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes)
Fig. 2. Transmission electron microscopy photographs of fresh sperm samples: (A) Spermatozoa longitudinal section showing the transitional region of the flagellum and the classic nine doublets of microtubules (9+0), arrow. (B) Longitudinal section of the head and midpiece of a spermatid. (C) Transversal section of the midpiece showing the axoneme surrounded by mitochondria. N: Nucleus; P: Proximal centriole; D: Distal centriole; CC: Citoplasmic Channel; M: Mitochondria; F: Flagellum.
Fig. 4 in Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes)
Fig. 4. Transmission electron microscopy photographs of pejerrey post thawed sperm showing different types of alterations. (A) Head with ruptured membranes (*). (B) Head with swollen and broken membrane (*). (C) Broken and swollen nuclear membrane (arrows) and alteration of the spatial orientation of mitochondria. (D) Ruptured and swollen membrane of the flagellum (arrows) with vesicles. (E) Tail around the head (arrows). N: Nucleus; M: Mitochondria; V: Vesicles.
Fig. 3 in Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes)
Fig. 3. Transmission electron microscopy photographs of the flagellum. (A) Flagellum axoneme showing the typical 9+2 doublets of microtubules. (B) Transversal section of the flagellum showing the lateral extensions like "fins" in both sides (arrows). (C) Longitudinal section of the flagellum. F: Flagellum.
FIGURE 5 in Seminal characteristics and sensitivity of Astyanax lacustris (Characiformes: Characidae) sperm to cryoprotective solutions based on dimethylsufoxide and methylglicol
FIGURE 5 | Compressed cell nucleus found in T4 of cryopreservation process of Astyanax lacustris semen.
FIGURE 1 in Seminal characteristics and sensitivity of Astyanax lacustris (Characiformes: Characidae) sperm to cryoprotective solutions based on dimethylsufoxide and methylglicol
FIGURE 1 | Fluorescence photomicrograph of Astyanax lacustris (100X magnification) of nuclei with different types of damage assessed by the Comet Assay: damage 0 - no apparent damage; damage 1 - little damage; damage 2 - average damage; damage 3 - major damage.
Fig. 7 in Sperm evolution in the family Alestidae with comparative data for the genus Chalceus (Ostariophysi: Characiformes)
Fig. 7. Spermatozoon of Micralestes acutidens. a, a-inset: longitudinal sections. b, c, d: transverse sections from top to posterior region. The nucleus (n) is spherical and lies strongly eccentric to the flagellum (f). The centriolar complex (p, d) and nuclear fossa (arrow) is strongly eccentric. The proximal centriole (p) is lateral and perpendicular relative to the distal centriole (d). The cytoplasmic canal is present (asterisk). The midpiece (pi) is strongly asymmetrical, and contains the spherical mitochondria (m) and vesicles (v). The membrane of the midpiece (me) has a striated aspect along its length (a, a- inset). Legends: cr: concentric membranous rings, d: distal centriole, f: flagellum, m: mitochondria, me: membrane of the midpiece, n: nucleus, p: proximal centriole, v: vesicle, asterisk: cytoplasmic canal, arrow: nuclear fossa.
Fig. 1 in Sperm evolution in the family Alestidae with comparative data for the genus Chalceus (Ostariophysi: Characiformes)
Fig. 1. Spermiogenesis process of the Alestidae. Figure subunits are longitudinal sections of spermatids corresponding to (a, b) Brycinus lateralis, (c) Brycinus imberi, (d) Brachypetersius altus, (e) Alestopetersius compressus, (f) Micralestes acutidens. The flagellum is initially lateral to the nucleus. The nuclear rotation towards the flagellar axis is present. The nuclear rotation can be complete reaching 90 degrees as represented by the late spermatid of Alestopetersius compressus (e), or incomplete reaching about 20 degrees as occurs in the late spermatid of Micralestes acutidens (f). Legends: c: cytoplasm, d: distal centriole, f: flagellum, m: mitochondria, n: nucleus, p: proximal centriole, pi: midpiece, v: vesicle, asterisk: cytoplasmic canal, arrow: nuclear fossa.
Fig. 1 in Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm
Fig. 1. Motility rate (a); curvilinear (VCL; b), straight-line (VSL; c) and average path (VAP; d) velocities of Prochilodus lineatus sperm diluted in two extender compositions (ACP = closed circles; BTS = open circles) and stored at 6-8°C for six days. Undiluted sperm served as control (closed triangle). Each dot and error bar represents mean ± SD; n=15 males. ACP: Powdered Coconut Water™; BTS: Beltsville Thawing Solution™. *Means followed by this symbol, within the same day of analysis, are significantly higher (P<0.05; Tukey).
Fig. 1 in Fresh, equilibrated and post-thaw sperm quality of Brycon orbignyanus (Valenciennes, 1850) and Prochilodus lineatus (Valenciennes, 1837) treated with either salmon GnRHa and domperidone or pituitary extract
Fig. 1. Motility rate of fresh, equilibrated and post-thaw sperm of Brycon orbignyanus (A; n = 18 males) and Prochilodus lineatus (B; n = 21 males). Motility rate was evaluated after 0, 20, 40 and 60s (fresh and equilibrated sperm) or after 10, 30, 50 and 70s of activation (post-thaw sperm). *Motility evaluated at 60s was lower than that at 0s post-activation (Scott-Knott, P <0.05). § Motility evaluated at 10s post-activation was the highest (Scott-Knott, P <0.05).
Fig. 2 in Fresh, equilibrated and post-thaw sperm quality of Brycon orbignyanus (Valenciennes, 1850) and Prochilodus lineatus (Valenciennes, 1837) treated with either salmon GnRHa and domperidone or pituitary extract
Fig. 2. Post-thaw sperm velocities (curvilinear = VCL; straight-line = VSL; average path = VAP) of Brycon orbignyanus (A; n = 18 males) and Prochilodus lineatus (B; n = 21 males) evaluated after 10, 30, 50 and 70s after activation. * Mean at 10s was the highest (Scott-Knott, P <0.05).
Рис. 2. Морфотипы Зрелых сперматоЗоидов (СЭМ) у Mytilus trossulus (A–G), Crenomytilus grayanus (H–L) и Mytilus coruscus (M–R): a – акросома; br – basal ring; n – Ядро; m – митохондриЯ; fl – Жгутик. Масштаб линейки – 1 мкм. in Study on sperm heteromorphism in some mussels (Bivalvia: Mytilidae) from the Sea of Japan
Рис. 2. Морфотипы Зрелых сперматоЗоидов (СЭМ) у Mytilus trossulus (A–G), Crenomytilus grayanus (H–L) и Mytilus coruscus (M–R): a – акросома; br – basal ring; n – Ядро; m – митохондриЯ; fl – Жгутик. Масштаб линейки – 1 мкм.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.