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1,149 results for “transgenic”
Targeted sequencing of T-DNA borders in OCP1xOGC transgenic lines of Camelina
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Computationally defined and in vitro validated putative genomic safe harbour loci for transgene expression in human cells
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GluN2B-specific NMDAR positive allosteric modulation reverses cognitive and behavioral abnormalities in Mecp2 and Disc1 transgenic mice
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Abiotic environments can modify the penetrance of transgene-based lethality systems for insect population control
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Data from: The genetic structure of Asian corn borer, Ostrinia furnacalis, populations in China: haplotype variance in Northern populations and potential impact on management of resistance to transgenic maize
Asian corn borer, Ostrinia furnacalis (Guenée), is a severe pest that infests cultivated maize in the major production regions of China. Populations show genotype-by-environment variation in voltinism, such that populations with a single generation (univoltine) are fixed in Northern China where growing seasons are short. Low genetic differentiation was found among samples from 33 collection sites across China and one site from North Korea (n = 1,673) using variation at 6 nuclear microsatellite loci (ENA corrected global FST = 0.020; P-value < 0.05). Analysis of molecular variance (AMOVA) indicated that geographic region, number of generations or voltinism accounted for < 0.38% of the total genetic variation at nuclear loci and was corroborated by clustering of co-ancestries among genotypes using the program STRUCTURE. In contrast, a mitochondrial haplotype network identified four distinct clusters, where 70.5% of samples from univoltine populations were within a single group. Univoltine populations were also placed into a unique cluster using Population Graph and Principal Component analyses, which showed significant differentiation with multivoltine populations (ST = 0.400; P-value < 0.01). This study suggests that gene flow among O. furnacalis in China may be high among regions, with the exception of northeastern localities. Haplotype variation may be due to random genetic drift resulting from partial reproductive isolation between univoltine and multivoltine O. furnacalis populations. Such reproductive isolation might impact the potential spread of alleles that confer resistance to transgenic maize in China.
Data from: Rapid growth accelerates telomere attrition in a transgenic fish
Background: Individuals rarely grow as fast as their physiologies permit despite the fitness advantages of being large. One reason may be that rapid growth is costly, resulting for example in somatic damage. The chromosomal ends, the telomeres, are particularly vulnerable to such damage, and telomere attrition thus influences the rate of ageing. Here, we used a transgenic salmon model with an artificially increased growth rate to test the hypothesis that rapid growth is traded off against the ability to maintain somatic health, assessed as telomere attrition. Results: We found substantial telomere attrition in transgenic fish, while maternal half-sibs growing at a lower, wild-type rate seemed better able to maintain the length of their telomeres during the same time period. Conclusions: Our results are consistent with a trade-off between rapid growth and somatic (telomere) maintenance in growth-manipulated fish. Since telomere erosion reflects cellular ageing, our findings also support theories of ageing postulating that unrepaired somatic damage is associated with senescence.
CRISPR-Cas12a-integrated transgenes in genomic safe harbors retain high expression in human hematopoietic iPSC-derived lineages and primary cells
<p>We identified and characterized potential integration safe harbor sites (SHS) in human cells. Using the CRISPR-MAD7 system, we integrated transgenes at these genomic sites in iPSC, primary T and NK cells, and Jurkat cell line, and demonstrated efficient and stable expression at these loci. Subsequently, we validated the differentiation capabilities of engineered iPSC towards CD34+ hematopoietic stem and progenitor cells (HSPC), lymphoid progenitors (LPC), and natural killer (NK) cells, and showed that transgene expression was retained in these lineages. </p>
Confocal stacks of Cii_PC2_hM4D(Gi)_mCherry transgenic Ciona Larvae
<p>Data related to Supplementary Figure 5 of the manuscript titled: <strong>Polymodal sensory perception drives robust attachment and metamorphosis of a pre-vertebrate zooplanktonic larva. </strong></p>
Data and code for: The complete Kaposi Sarcoma-associated herpesvirus genome induces early-onset, metastatic angiosarcoma in transgenic mice
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Characterisation of large transgene integrations in Chinese hamster ovary cells using a bioengineered mammalian transposase
<p>Supporting information for "Characterisation of large transgene integrations in Chinese hamster ovary cells using a bioengineered mammalian transposase"</p>
Genes Downregulated in the ABA+DEX group in the 35S:AtbZIP62-GR transgenic plants
<p>Genes down regulated in ABA+DEX treated seedlings compared with ABA treated seedlings in the 35S:AtZIP62-GR transgenic plants.</p>
Data from: Predation, metabolic priming and early life-history rearing environment affect the swimming capabilities of growth hormone transgenic rainbow trout
The period of first feeding, when young salmonid fishes emerge from natal stream beds, is one fraught with predation risk. Experiments conducted in semi-natural stream mesocosms have shown that growth hormone transgenic salmonids are at greater risk of predation than their non-transgenic siblings, due partly to the higher metabolic demands associated with transgenesis, which force risky foraging behaviours. This raises questions as to whether there are differences in the swim-performance of transgenic and non-transgenic fishes surviving predation experiments. We tested this hypothesis in wild-origin rainbow trout (Oncorhynchus mykiss) that were reared from first feeding in semi-natural stream mesocosms characterized by complex hydrodynamics, the presence of predators and oligotrophic conditions. Using an open-flume raceway, we swam fish and measured their capacity for burst-swimming against a sustained flow. We found a significant genotype effect on burst-performance, with transgenic fish sustaining performance longer than their wild-type siblings, both in predator and predator-free stream segments. Importantly, this effect occurred before differences in growth were discernable. We also found that mesocosm-reared fish had greater burst-performance than fish reared in the controlled hatchery environment, despite the latter being unexposed to predators and having abundant food. Our results suggest a potential interaction between predation and metabolic priming, which leads to greater burst capacity in transgenic trout.
Ozone modified hypothalamic signaling enhancing thermogenesis in the TDP-43A315T transgenic model of Amyotrophic Lateral Sclerosis
<p><strong>Illumina sequencing and analysis</strong></p>
Impact of transgenic maize Ruifeng125 on diversity and dy-namics of bacterial community in rhizosphere soil
<p><span>With the development of commercialized planting of genetically modified crops, their ecological security risks remain a hot topic of public concern. Insect-resistant genetically modified maize Ruifeng125 which expressing a fusion Bt protein (Cry1Ab-Cry2Aj) has obtained the application safety certificate issued by the Chinese government. To determine the effects of Ruifeng125 on the diversity and dynamics of bacterial communities, the accumulation and degradation pattern of the fusion Bt protein in the rhizosphere soil of transgenic maize were detected firstly. Results showed that the contents of Bt protein varied significantly at different developmental stages, but after straw returning to the field, over 97% of Bt proteins has been degraded quickly at the early stages (≤ 10 d) and then they were degraded at a relative slow rate. In addition, the variations of bacterial community diversity in the rhizosphere soil were detected by 16S ribosomal RNA (Rrna) high-throughput sequencing technology. A total of 44 phyla, 435 families and 842 genera were obtained by 16S rRNA sequencing, among which <em>Proteobacteria</em>, <em>Actinobacia</em>, <em>Acidobacter</em> <em>Acidobacterium</em> and <em>Chloroflexi</em> were the dominant taxa. At the same developmental stage, no significant differences in soil bacterial diversity were detected between Ruifeng125 and its non-transgenic control variety. Further analysis revealed that developmental stage, rather than the transgenic event, made the greatest contribution for the changes in soil microbial diversity. This research provides important information for evaluating the impacts of Bt crops on the soil microbiome and establish a theoretical foundation for their environmental safety assessment.</span></p>
Data: Limiting hearing loss in transgenic mouse models
<p>A collection of post-processing data chunks used for generating figures for the publication <em>Limiting hearing loss in transgenic mouse models. </em>Each folder corresponds to an individual mouse involved in the study and includes datum generated from widefield and two-photon imaging (ROIs, flourescence values, etc.).</p>
Smart-Watch-Programmed Green-Light-Operated Percutaneous Control of Therapeutic Transgenes
<p>Data underlying the figures in the publication “Smart-watch-programmed green-light-operated percutaneous control of therapeutic transgenes”, published in <em>Nat. Commun.</em>, <strong>2021</strong>, 12, 3388.</p> <p><a href="https://doi.org/10.1038/s41467-021-23572-4">https://doi.org/10.1038/s41467-021-23572-4</a></p> <p>Table of contents:</p> <p><strong>1. Dataset</strong>; Excel file containing the numerical data for <em>Figures 1c, 2b, 2c, 3a-f, 4a, 4c, 5b, 5d, 6b-h </em>as well as for<em> Figures S1C, S3a-c, S4, S5b, S6b, S7c, S8b, S12c, S13b, S14 </em>and <em>S15b-d.</em></p> <p> </p> <p> </p>
The common bean aquaporin gene PvXIP1;2 identified by GWAS at seedling stage confers drought and osmotic resistance in transgenic plants
<p>This is the trait data of survival rate used to conduct a GWAS</p>
Fig. 6 in The effect of β-cyclocitral treatment on the carotenoid content of transgenic Marsh grapefruit (Citrus paradisi Macf.) suspension-cultured cells
Fig. 6. Changes in metabolite pools of suspension-cultured cells resulting from fourteen days β-cyclocitral (0.5 mM) treatment. Asterisk indicate significance of difference at *P value <0.05, **P <0.01 and ***P <0.001, respectively. Red coloured asterisks indicate the relative metabolite contents increased by β-cc treatment whilst green represent a decrease. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 3 in The effect of β-cyclocitral treatment on the carotenoid content of transgenic Marsh grapefruit (Citrus paradisi Macf.) suspension-cultured cells
Fig. 3. Effect of twelve days treatment with different concentrations of β-cyclocitral on growth index of citrus suspension-cultured cells. The letters represent significantly different groups following ANOVA.
Fig. 1 in The effect of β-cyclocitral treatment on the carotenoid content of transgenic Marsh grapefruit (Citrus paradisi Macf.) suspension-cultured cells
Fig. 1. Carotenoid biosynthesis pathway and the precursors for β-cyclocitral synthesis in plants. GGPP, geranylgeranyl diphosphate; PSY, phytoene synthase; PDS, phytoene desaturase; Z-ISO, ζ-carotene isomerase; ZDS, ζ-carotene desaturase; CRTISO, carotenoid isomerase; LCYe, lycopene ε-cyclase; LCYb, lycopene β-cyclase; BCH, carotene β-hydroxylase; CYP97C, cytochrome P450-type monooxygenase 97C (carotene ε-hydroxylase); ZEP, zeaxanthin epoxidase; VDE, violaxanthin de-epoxidase.
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.